University of Udine
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Predictive Factors for Failed Sentinel Lymph Node Mapping in Endometrial Cancer: A Retrospective Multicenter Study
No full textBackground and Objectives: This study aims to evaluate the predictive factors associated with failed sentinel lymph node (SLN) mapping in a large, retrospective cohort of patients with early-stage endometrial cancer (EC). Methods: We retrospectively evaluated a series of EC patients who underwent laparoscopic SLN mapping with intracervical indocyanine green (ICG) injection in five referred oncological centers from January 2019 to March 2024. We compared the clinical and pathological features of bilateral and failed SLN mapping, which was defined as either unilateral mapping or no SLN mapping. Logistic regression was used to identify predictors of failure. Results: Among 623 analyzed patients, 437 (70.14%) had a successful bilateral procedure. On univariate analysis, age (p = 0.03), non-endometrioid histology (p = 0.02) and previous vaginal delivery (p = 0.015) were significant associated with failed SLN mapping. On multivariable analysis, only increasing age (OR 1.03; 95% CI, 1.01–1.04, p = 0.03) and non-endometrioid histology (OR 1.81; 95% CI, 1.01–3.19) were independently associated with unsuccessful procedure. No significant differences were observed for BMI, enlarged lymph nodes, intraoperative lysis of adhesion, LVSI, grade 3, and FIGO stage. Conclusions: Increasing age and non-endometrioid histology are independent predictors of bilateral SLN mapping failure in EC patients undergoing SLN mapping with cervical ICG injection
The Gut Microbiome as a Possible Mediator in Autoimmunity and Cardiovascular Disease: Shared Pathways and Therapeutic Implications
No full textThis review explores the emerging role of the gut microbiome in bridging autoimmunity and cardiovascular diseases. Dysbiosis, an imbalance in gut microbial composition, disrupts immune regulation, metabolic pathways, and vascular health, likely contributing to both autoimmune disorders and cardiovascular diseases. Microbial metabolites, such as short-chain fatty acids, trimethylamine N-oxide, tryptophan derivatives, and bile acids, play critical roles in modulating inflammation, lipid metabolism, and endothelial function. Specific bacterial species, including Faecalibacterium prausnitzii, Akkermansia muciniphila, and Bifidobacterium species., exhibit dual protective effects against autoimmune and cardiovascular pathologies. By elucidating these interconnected mechanisms, this work highlights the potential of microbiome-targeted therapies, such as probiotics, prebiotics, and dietary interventions, to concurrently address autoimmune diseases and reduce cardiovascular risk. Understanding the complex interactions between the gut microbiota, immune system, and cardiovascular health opens new avenues for developing innovative therapeutic strategies aimed at restoring microbial balance and improving patient outcomes
«Dipinti e cari ricordi». La collezione di pittura di una villa nel Friuli occupato della grande guerra.
No full textIl caso di una collezione privata di dipinti depredata durante la guerra guerra è occasione di considerare i diversi piani personali e collettivi sui quali si registrano le drammatiche conseguenza del conflitto in Friuli
Influence of Coffee Roasting Degree from Four Mexican Regions on In Vitro Antioxidant Activity and Digestive Enzyme Inhibition and Its In Vivo Effects on Carbohydrate and Lipid Absorption
No full textCoffee is among the most consumed beverages worldwide and is recognized for its bioactive compounds, which exert diverse physiological effects. This study evaluated the impact of roasting degree on the in vitro antioxidant activity and digestive enzyme inhibition of brews from four Mexican regions, as well as their in vivo effects on carbohydrate and lipid absorption. Antioxidant capacity was assessed through radical scavenging and lipid peroxidation inhibition, while inhibition of lipase, α-amylase, and α-glucosidase was also determined. Oral starch (OSTT) and lipid (OLTT) tolerance tests were conducted in healthy Wistar rats. Antioxidant activity was strongly influenced by region and roasting degree. All coffee samples exhibited radical scavenging activity and lipid peroxidation inhibition. With respect to enzyme inhibition, all coffees showed ~67–70% inhibition of lipase activity. For amylase, unroasted coffee from Oaxaca displayed the highest inhibition (34%, p < 0.001). For glucosidase, unroasted samples showed low inhibition (~6–19%), which increased substantially at the medium roast degree (~55% across all samples) but decreased again at the high roast degree (~27%). In OSTT, serum glucose levels were reduced after 120 min by ~20%, 21%, and 18% in rats treated with unroasted, medium-roast, and high-roast coffee, respectively, compared with the negative control. In OLTT, serum triglycerides decreased by ~26% (Chiapas), ~58% (Colima), ~32% (Oaxaca), and ~54% (Hidalgo). Crop region and roasting degree influence the phytochemical profile and bioactivity of Mexican coffee. Although unroasted coffees had the highest concentration of bioactive compounds, roasting enhanced specific bioactivities, particularly enzyme inhibition and lipid-lowering effects in vivo
EcDNA replication is disorganized and vulnerable to replication stress
No full textExtrachromosomal DNA (ecDNA) is a critical driver of cancer progression, contributing to tumour growth, evolution, and therapeutic resistance through oncogene amplification. Despite its significance, the replication of ecDNA remains poorly understood. In this study, we investigated the replication dynamics of ecDNA using high-resolution replication timing analysis (Repli-seq) and DNAscent, a method for measuring origin firing and replication fork movement, that we applied to both bulk DNA and to ecDNA isolated with FINE (Fluorescence-activated cell sorting-based Isolation of Native ecDNA), a new method for isolating, chromatinized ecDNA without DNA or protein digestion. We demonstrate that ecDNA in the COLO 320DM colorectal cancer cell line exhibits largely asynchronous replication throughout the S phase, contrasting with the conserved replication timing of the corresponding chromosomal DNA in RPE-1 cells and the chromosomally reintegrated ecDNA in COLO 320HSR. Replication origins on ecDNA are redistributed, and replication forks exhibit reduced velocity and increased stalling. Under replication stress induced by hydroxyurea treatment, ecDNA replication is further compromised, leading to altered origin activation, reduced fork velocity and eventual ecDNA depletion from cells. Our findings reveal fundamental differences in the replication dynamics of ecDNA, providing insights that could inform the development of therapies targeting ecDNA-associated oncogene amplification in cancer
Impact of a High-Fat High-Carbohydrate (HFHC) Diet at a Young Age on Steroid Hormone Hair Concentrations in Mice: A Comparison with a Control Diet and Nutraceutical Supplementation
No full textAn unhealthy prepubertal diet can have long-lasting effects throughout life. This study
investigated hair concentrations of adrenal and sex steroids, in an in vivo mouse model
of juvenile obesity subjected to control (CTRL), obesogenic (HFHC) diet, or nutraceutical
supplementation (silymarin or coconut oil) diets. 87 3-week-old C57BL/6 mice
(42 females, 45 males) were fed CTRL or HFHC diets for 8 weeks. Afterward, the
CTRL group continued on CTRL diet while the HFHC diet group was divided into five
groups: HFHC, HFHC→CTRL, HFHC→CTRL + silymarin (SIL), HFHC→HFHC + SIL
and HFHC→HFHC + Coconut oil. At 4 weeks, the HFHC group showed increased cortisol/
dehydroepiandrosterone (DHEA) ratio compared to CTRL group. At 20 weeks, the
HFHC→HFHC group showed higher levels of progesterone (P4) and dehydroepiandrosterone
sulfate (DHEA-S) and lower levels of estradiol (E2) compared to the CTRL→CTRL
group. The switch from HFHC→CTRL was the optimal therapy because the body weight
and almost all the hormones were close to those observed for the CTRL diet group. Supplement
with SIL or Coconut oil reduced DHEA-S and increased in E2 compared with the
endocrine setting seen with the HFHC diet. These interventions should be considered as
supportive measures rather than substitutes for dietary correction
Reconsidering Integrated Reporting in Public Sector Organisations: Challenges for Territorial Governments
No full textThe past three decades have seen the development and implementation of new reporting formats, including social, sustainability, and integrated reports to respond to the increasing demand for accountability within public sector organisations (PSOs). This chapter examines the case of integrated reporting (IR), which has emerged as a widely recognised standard for assessing value creation. Despite this, evidence suggests that its adoption remains sluggish within the public sector, particularly among territorial governments, that is, administrative bodies responsible for political direction and service provision within a defined geographical area, such as local, provincial, or regional authorities. The research focuses on how the characteristics of PSOs (with a focus on territorial governments) influence IR and what are the challenges it faces within this type of organisations. The key components of the IR framework are assessed through a deductive analysis in relation to the distinctive features of traditional public administration settings. The implications of the findings are discussed to provide suggestions for future research and practice
Urinary Tumor DNA–based Liquid Biopsy in Bladder Cancer Management: A Systematic Review
No full textBackground and objective: Urinary tumor DNA (utDNA) has emerged as a promising biomarker in the care, diagnosis, early detection, recurrence monitoring, and prognosis of bladder cancer (BCa). Its noninvasive nature, ease of access, and cost effectiveness make it an attractive option for both patients and health care providers. This review describes the current state of utDNA as a marker of BCa. Methods: Articles published between 2015 and 2025 on current utDNA-based techniques in BCa were identified and analyzed for relevance and insight into utDNA research and usage. Key findings and limitations: Recent investigations underscore the noninvasiveness and superior tumor detection capabilities of utDNA, particularly in the detection of minimal residual disease. Moreover, utDNA provides actionable information, such as tumor grade and staging information, to support precise treatment decisions, including targeted immunotherapy regimens and bladder preservation strategies. Although utDNA has shown promising results in small studies, larger studies must be performed before it can be considered as a standard procedure in clinical practice. Conclusions and clinical implications: Urinary tumor DNA has demonstrated great potential to improve on most, if not all, stages of detection, treatment, and monitoring of BCa. By preserving the low cost and noninvasiveness of urine cytology, and by replacing its suboptimal accuracy with a precision rivaling and often exceeding cystoscopy and circulating tumor DNA–based methods, utDNA offers patients a more comfortable, repeatable, and accurate way of detecting BCa. With increased sensitivity and accuracy, everything from low-grade tumors to the earliest signs of recurrence can be detected more effectively, optimizing patient treatment courses and improving outcomes
DIRITTO PERDUTO
No full textIntervista di Marco Lincetto, titolare del Canale di Informazione su You Tube "Il Punto", su tematiche giuridiche varie di attualità.
Minutaggio: 00':00'' - 36':02'