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SOVRANE ORIENTALI re. ad Angela Vignotto, Imperatrici dell’Impero Romano d'Oriente (324-1453), Roma 2023.
No full textSguardo su Costantinopoli attraverso gli occhi delle donne, specificamente delle imperatrici. Un contributo che fornisce una chiave di lettura che non solo colma una lacuna della storiografica, ma mostra quando, come e perché l’elemento femminile del potere va a corroborare l’azione maschile e persino a indirizzarla o addirittura a sostituirla
Nuovi dilemmi comunicativi. Le sfide della professione giornalistica alla prova con l’IA
No full text3D Electrospun Scaffolds Functionalized with Amniotic Epithelial Stem Cell Secretome as Immune-Responsive Platform for Cell-Free Tendon Regeneration
No full textTendon repair is often compromised by chronic inflammation and disrupted immune balance, which limit current treatment outcomes and call for advanced regenerative approaches [1]. To address this, an immune-informed system was developed by functionalizing validated 3D tendon-mimetic PLGA scaffolds [2] with immunomodulatory conditioned media (CMINF ) derived from ovine amniotic epithelial stem cells (AECs), which are well recognized for their paracrine and immunoregulatory properties [3]. This cell-free approach was explored through three functionalization techniques: physical adsorption, HCl and NaOH pre-treatments. FT-IR spectroscopic and protein adsorption analyses identified NaOH treatment as the most effective method, promoting enhanced scaffold reactivity and improved retention and release of Amphiregulin (AREG), a key immunomodulatory factor a molecule implicated in tissue repair and M2 macrophage polarization [4]. Release studies demonstrated that 3D-CM INF scaffolds ensured a bioactive, sustained delivery of AREG over a 7-day period. Functionally, 3D-CM INF scaffolds inhibited significantly T-cell activation and suppressed PBMC proliferation. Moreover, conditioned media released from 3D-CM INF (CMR ) displayed timedependent immunomodulatory profiles, with early inhibition of T cells (6–72 h) and delayed suppression of PBMC proliferation (48 h–7 d). Importantly, macrophage polarization assays revealed a shift toward pro-regenerative M2 phenotype, with increased expression of M2 over M1 markers in 3D-CMINF -adherent cells. Flow cytometry showed a preferential induction of regulatory M2b macrophages, accompanied by reduced proportions of pro-inflammatory M1 and pro-fibrotic M2a subsets. Overall, these findings show that 3D-CM INF scaffolds can actively regulate immune responses by orchestrating both inflammatory resolution and tissue-regenerative signals. This integrative platform, merging structural and paracrine immunomodulation, offers a promising, cellfree, and clinically relevant immunoengineering strategy for tendon regeneration.
Keywords: Amniotic Epithelial Stem Cells; Conditioned Media; Scaffold Functionalization; Immunomodulation; Macrophages Polarization; Tendon regeneration.
Funding Acknowledgment: This work was supported by Progetti di Rilevante Interesse Nazionale (PRIN) – Smart Biomimetic Device for Tendon Tissue Engineering – CUP C53D23005440006
sentiwordnet_it 1.0 (v1.0.1) [Data set]
No full textThis repository contains a sentiment lexicon for Italian, based on SentiWordNet 3.0 (Baccianella, Esuli, and Sebastiani 2010; Esuli [2019] 2025) and MultiWordNet (Pianta, Bentivogli, and Girardi 2002)
Cystoisospora spp. infections and other intestinal parasitoses in dogs and cats with diarrhea in Italy and Greece
No full textOn Group Secrets and the Metacommunicative Aspects of Revealing a True Secret
No full textThis research paper explores the logical foundations of group secrets, focusing on their preservation and the interplay of belief, knowledge, and intention inherent in secrecy among agents. Key findings establish the logical conditions under which group secrets are maintained and examine how various logical operations influence these secrets. Additionally, the paper offers insights into certain metacommunicative aspects of secret revelation, specifically when the goal is leaving the secret unknown to a targeted nescient. In particular, we provide a formal analysis of intentions crucial in communicative acts
Impact of urea-molasses treated wheat straw levels in total mixed rations on growth and nutrient digestibility in Azikheli buffalo calves
No full textThis study investigates the impact of urea molasses-treated wheat straw on growth performance and nutrient digestibility in Azikheli buffalo male calves. A longitudinal experiment was conducted on sixteen calves, randomly assigned to four experimental dietary treatments (n = 4 per group). The experimental diets included a control group (U0) with untreated wheat straw and three groups where urea molasses-treated wheat straw replaced 33% (U33), 66% (U66), or 100% (U100) of the straw in the total mixed ration. Results indicated that increasing the proportion of urea-treated wheat straw from 0 to 100% led to significant improvements in dry matter intake (from 2931 to 4034 g/day) and organic matter intake (from 2596 to 3623 g/day). Digestibility of dry matter, organic matter, crude protein, and crude fiber also followed an increasing trend, reaching 77.42%, 81.21%, 87.10%, and 60.22%, respectively, at the highest urea-treated wheat straw level. Furthermore, weight gain was significantly greater in calves fed 100% urea-treated wheat straw, followed by those in the U66, U33, and U0 groups. Feed conversion efficiency improved substantially in the U100 and U66 groups than U33 and U0. These findings suggest that incorporating higher levels of UMTWS in total mixed rations enhances nutrient digestibility, promoting superior growth performance and feed efficiency in Azikheli buffalo calves
Novel sustainable strategies to mitigate toxicity of emerging contaminants: cellular and physiological insights from CMIT-exposed mussels treated with insect-based protein hydrolysates
No full textThe present interest in developing sustainable strategies to mitigate or prevent toxicity of emerging contaminants is attributable to their increasing occurrence in natural ecosystems. Beyond their established roles in the agri-food, feed, and biotechnology sectors, insects are emerging as valuable sources of bioactive compounds, highlighting their recognition as sustainable solutions in nutraceutical and pharmaceutical research. Therefore, the effects of protein hydrolysates from the black soldier fly, Hermetia illucens (BPHs) were evaluated against the toxicity induced by the methylchloroisothiazolinone (CMIT), a widely used biocides belonging to the isothiazolinones class of the biocides. Analyses focused on the modulation of key cellular and physiological parameters in Mytilus galloprovincialis exposed to CMIT (0.01 mg/L), BPHs (0.5 mg/mL), and their mix. Changes in haemocyte functions were assessed through cell viability assays and phagocytosis assay along with the expression of γ-actin; investigation into digestive gland (DG) functionality was carried out by measuring the viability of DG cells and their ability to perform osmoregulation after a hypotonic shock through the Regulatory Volume Decrease video-metric assay (RVD); antioxidant and cytoprotective responses were evaluated through the Cu/ZnSOD, MnSOD, Hsp70, and CYP4Y1 gene expression analysis. Our findings showed that BPHs play a significant protective role against toxicity induced by CMIT in exposed mussels. Significant differences emerged between the control and the CMIT-treated groups, whereas a similar trend was observed between the CTRL and BSPHs-exposed groups. Groups exposed to the combinations (BPHs + CMIT) showed a recovery, suggesting the protective effect of this compound