24663 research outputs found
Sort by
Apparenza e Rivoluzione. La politica della moda nella Francia rivoluzionaria
No full textObiettivo di questo contributo è riflettere su quei momenti della Rivoluzione francese durante
i quali abiti e simboli furono usati come strumenti di lotta politica. Gli eventi rivoluzionari si svolsero
anche attraverso l’atto di indossare o imporre un abito o un simbolo. Travestirsi o accusare
qualcuno di essersi appropriato dell’identità altrui era una parte importante del confronto politico,
così come la pratica di denudare il proprio avversario
New insights into the crosstalk between endocannabinoids and sphingosine-1-phosphate
No full textThis review aims at highlighting the interplay between the endocannabinoids (eCBs) anandamide and 2-arachidonoylglycerol and sphingosine-1-phosphate (S1P) signaling. The eCBs and S1P are bioactive compounds that exemplify a paradigm of crosstalk among lipid signals, with profound implications for physiological processes and disease pathogenesis. Crosscommunication between eCBs and S1P occurs through multiple mechanisms: (i) receptor heterodimerization and coregulation, (ii) mutual metabolic modulation, and (iii) integrated regulation of downstream effectors. The latter emerged as a key mechanism underlying the bidirectional interactions between eCBs and S1P, with functional overlaps that regulate several processes, including inflammation, vascular function, and neuronal activity. In addition, cannabis-derived compounds (such as cannabidiol) can influence eCBs and S1P signaling, calling for further research into their therapeutic exploitation. Overall, the dynamic interplay between endogenous eCBs and S1P—as well as with exogenous cannabidiol—described here offers a compelling example of the complexity of interactions among bioactive lipids. A deeper mechanistic understanding of these relationships could pave the way to novel strategies in drug design and development, emphasizing the importance of integrated approaches in the study of bioactive lipid biochemistry
Recensione a Paolo Ferretti, Racconti romanistici, Il diritto romano in dodici casi, Editore Giappichelli, Torino 2024
No full textIl volume sollecita ancora una riflessione sull'importanza dell'esperienza giuridica romana che non è solo un'eredità storica di incomparabile valore ma è altresì un modello di pensiero e di astrazione che troviamo permutato nelle codificazioni contemporanee e che costituisce ancora oggi un elemento formativo irrinunciabile per l’avvocato e per il giurista. Una continuità storica e metodologica che, passando dall’esercizio logico sui casi e sulle discussioni, esplica tutt’ora la sua validità e la sua profondità di analisi e di ragionamento
Opposing Roles of KLHL14 and X1 Isoforms in the Regulation of E-Cadherin-mediated Epithelial-Mesenchymal Transition
No full textKLHL14 belongs to the Kelch-like (KLHL) family of E3 ubiquitin ligases. Proteins of this family share a conserved dimerization domain (BTB), a linker domain (BACK), and a C-terminal Kelch domain responsible for substrate recognition [1]. KLHL14 has recently emerged as a tumor suppressor in several cancers [2, 3]. Although it was originally implicated in the inhibition of epithelial-mesenchymal transition (EMT) in amniotic epithelial stem cells (AECs), its precise role and molecular mechanisms remain unclear [4]. Understanding KLHL14 function is highly relevant, as EMT is involved not only in cancer progression but also in stem cell development and tissue regeneration. Moreover, KLHL14 is expressed as two isoforms: the full-length form (KLHL14) and a short splice variant known as X1, which lacks the substrate-binding domain. However, the role of X1 has remained completely unexplored to date.
To elucidate the roles of KLHL14 and X1 in EMT, we employed two complementary models: AECs, a physiological EMT model, and HepG2, an epithelial hepatocarcinoma cell line with high endogenous KLHL14 expression. We investigated the contribution of both isoforms through gain- and loss-of-function approaches using c-Myc-tagged overexpression constructs and isoform-specific siRNA-mediated silencing.
Our results show that both isoforms contribute to the maintenance of epithelial identity through modulation of E-Cadherin, via two distinct mechanisms. Full-length KLHL14, predominantly localized at the plasma membrane in AECs and HepG2 cells, co-localized with E-Cadherin at adherens junctions. This result was further confirmed in situ in the whole amniotic membrane (AM). In contrast, X1 exhibited an endoplasmic reticulum (ER)-like pattern. Co-immunoprecipitation confirmed the specific interaction of both isoforms with E-Cadherin. This interaction was also observed in the AM, supporting the physiological relevance of our findings. Mechanistically, KLHL14 promotes E-Cadherin degradation via the ubiquitin-proteasome and lysosomal pathways, whereas X1 enhances its de novo synthesis. Functionally, silencing of both isoforms leads to overall E-Cadherin downregulation, increased cell proliferation, and EMT induction. Remarkably, these effects are conserved in the native AM, where silencing reduces E-Cadherin expression, disrupts adherens junctions, and increases Vimentin levels.
Overall, this study reveals the opposing functions of KLHL14 and X1 in balancing E-Cadherin-mediated EMT regulation. While KLHL14 promotes E-Cadherin degradation, X1 supports its expression, together modulating epithelial stability. These findings uncover a previously unrecognized regulatory axis and underscore KLHL14 as a crucial pivot for epithelial integrity and plasticity. Elucidating this mechanism opens new perspectives for targeting EMT in regenerative strategies and anti-cancer therapies, where reprogramming cell fate and preventing aberrant transitions remain major clinical challenges
Extracellular Vesicles Profiling in Acute Myeloid Leukemia Cell Lines: A Proteomic Characterization
No full textUltrasonographic and elastographic assessment of cervical changes throughout the canine oestrous cycle
No full textGoverning Consensus The Political Use of Knowledge in Italy
No full textHow were political scenarios opened up by the revolutionary era navigated? This volume investigates how expertise emerged as a vital political resource in the Italian peninsula between 1789 and 1870, a time of revolution and institutional upheaval. Moving beyond the familiar categories of charisma and celebrity, it explores the political role of knowledge by focusing on places of education, media events, agents and texts. Framing Italy as a laboratory of early modern politics, this volume examines how the reworking of traditional knowledge and the rise of new forms of expertise enabled individuals to act effectively in the public sphere, influence society and navigate the shifting landscape of power, ultimately governing consensus and securing lasting authority
