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Synergic use of essential oil and sous-vide packaging for ready-to-cook organic vs. conventional globe artichoke heads
No full textLA COSTRUZIONE DEL TERRITORIO FIORENTINO: POLITICHE EDILIZIE E CONTROLLO DELLO SPAZIO (1355-1415)
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Development of Amniotic Epithelial Stem Cells Secretome-Loaded In Situ Inverse Electron Demand Diels–Alder-Cross-Linked Hydrogel as a Potential Immunomodulatory Therapeutical Tool
No full textAmniotic epithelial stem cells (AEC) hold potential for tissue regeneration, especially through their conditioned medium (AEC-CM) due to their immunomodulatory and regenerative effects. Nevertheless, advanced drug delivery systems such as hydrogels are needed to enable clinical applications. Herein, an in situ gellable hyaluronic acid and polyethylene glycol-based iEDDA-cross-linked hydrogel was developed for the encapsulation and controlled release of AEC-CM. The developed system was formed by norbornene-modified hyaluronic acid and tetrazine-modified polyethylene glycol functionalized with heparin. The hydrogel was formed by mixing both precursor polymers, displaying fast cross-linking kinetics and showcasing a highly porous inner structure and low swelling properties. Moreover, the heparin-functionalized system allowed the sustained release of predominant growth factors from AEC-CM over 14 days. In vitro studies in peripheral blood mononuclear cells (PBMCs) showed an enhanced suppression efficacy and a significant shift toward the M2 macrophage phenotype in comparison with nonencapsulated AEC-CM. Therefore, this work provides a suitable alternative for the encapsulation of AEC-CM in a hydrogel formulation, highlighting its potential as an alternative immunomodulatory therapeutic tool for tissue regeneration
Interlaboratory validation trial report on multiplex real-time PCR method for molecular serotyping and identification of the 30 major clonal complexes of Listeria monocytogenes circulating in food in Europe
No full textThe performance of a new method developed in 2021 by the European Union Reference Laboratory (EURL) for Listeria monocytogenes based on 12 multiplex real-time PCR, allowing the identification of the molecular serotype and the 30 major L. monocytogenes multilocus sequence typing clonal complexes (CC), was assessed through a European interlaboratory validation trial (ILVT). This ILVT was adapted from ISO standard 16140 part 6. Overall, 98 blinded pure strains of Listeria (monocytogenes or spp.), previously characterized by the EURL, were sent to 15 laboratories distributed in 11 countries. The molecular serotype had to be identified for 20 strains of the ILVT panel, while CC identification had to be performed for the whole panel. The results of the 12 multiplex real-time PCR were reproducible between the participating laboratories with high individual concordance values for molecular serotyping (100%) and CC identification (90.8%-100%) irrespective of DNA extraction protocols, PCR master mixes, and thermocycler diversity. Master mixes identified as incompatible with some of the multiplex real-time PCR were excluded from the method. The overall concordance of the results was sufficient for the method to be confidently applied in other laboratories involved in L. monocytogenes typing.IMPORTANCEThis interlaboratory validation trial, coordinated by the European Union Reference Laboratory for Listeria monocytogenes, was the final step to assess the performance of the multiplex real-time PCR method developed and published by B. F & eacute;lix, K. Capitaine, S. Te, A. Felten, et al. (Microbiol Spectr 11:e0395422, 2023, https://doi.org/10.1128/spectrum.03954-22). Different combinations of parameter settings were applied in 15 French and European laboratories involved in L. monocytogenes typing. It was a prerequisite to establish this new real-time PCR method as a standard for rapid molecular serotyping and clonal complex identification. The accuracy and reproducibility of the results obtained on the panel of 98 strains of L. monocytogenes sent to the participants proved that the real-time PCR was suitable for use in their conditions. Rapid screening of strains is therefore now possible, and the method provides a valuable tool for epidemiological investigations to identify food-associated strains during listeriosis outbreaks
Transcriptomic Profile of Early Antral Follicles: Predictive Somatic Gene Markers of Oocyte Maturation Outcome
No full textEquine Distal Limb Wounds: Economic Impact and Short-Term Prognosis of Non-Synovial Versus Synovial Lesions in Southern Germany
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Two years of precision livestock management: harnessing ear tag device behavioral data for pregnancy detection in free-range dairy cattle on silage/hay-mix ration
No full textIntroduction: The aim of this study was to evaluate the transformative potential of precision livestock farming (PLF) in dairy production, nutrition, and reproductive efficiency by implementing proactive management strategies that utilize real-time data. Methods: Data were gathered from a commercial dairy farm, employing a 3D accelerometer system attached to ear-tags to monitor activity and rumination behavior. Additionally, information on animal-related events such as oestrus, artificial insemination, clinical diseases, and treatments was recorded. Results: It was observed that there were differences in rumination between positive and negative cows in the days following artificial insemination (AI). Specifically, from day 0 to day 16, negative cows exhibited a higher rumination time compared to positive cows, with this contrast being particularly significant on days 9 and 10. Over the duration of the study, cows that tested negative produced, on average, 0.78 kg more milk per day. Discussion: This suggests that animals with higher milk production were less likely to be pregnant, potentially due to increased nutrient allocation to the mammary gland, resulting in a more pronounced negative energy balance and consequently lower conception rates. Furthermore, the accelerometer utilized in this study was capable of distinguishing differences in daily lying time between pregnant and non-pregnant animals on the days of oestrus return. This finding highlights the potential for developing a system alert to identify “probable pregnant” and “probable non-pregnant” animals