1,901 research outputs found

    An ecophysiological approach to crop losses exemplified in the system wheat, leaf rust, and glume blotch

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    The motive of the author to embark upon the research reported here was the need to develop new concepts to approach the problem of crop losses, and eventually the problem of their prevention by means of disease control and plant breeding. The introduction of ecophysiology as an approach, explained above, was the first result. An ecophysiological treatment of processes like growth and development of plants in health and disease necessitates adjustments of current thoughts on experimental techniques and on organization in research (Van der Wal and Cowan, 1974).In order to measure plant or aegricorpus responses concurrently with environmental factors in climate chamber and field experiments during periods Of months, a great variety of instruments had to be bought, modified, or devised. The climate chamber had to be adjusted to allow for high light intensities with a view to growing wheat under conditions approximating those of early summer in the Netherlands; the yield of the spring wheat 'Kolibri' was c. 0.5 kg.m -2, which is nearly equivalent to the average field production of the country. Regulations for the prevention and control of contamination by aphids and mildew without any use of chemicals were issued after detailed experimentation (Van der Wal, unpubl.). Several instruments have been developed, usually with the help of others; this has led to two publications (Schurer and Van der Wal, 1972; Tegelaar and Van der Wal, 1974). These efforts resulted in the experimental designs described in the appendix. Field experiments, conducted in the same period as the climate chamber experiments reported here, will be published later. It is felt that the techniques to record growth of pathogens are still inadequate.The results reported in the appendix show that the 'state' of the plant at the time of infection is a major determinant of the plant's future behaviour, which plant breeders and pathologists can express in terms of resistance and tolerance. The conceptual framework presented above may contribute to a future revision of crop husbandry and crop protection practices

    P.P. van S., De Seldsaame en Noit Gehoorde Wal-Vis-Vangst, Voorgevallen by St. Anna-Land in't jaar 1682 (1684)

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    Diplomatische weergave van: De Seldsaame en Noit Gehoorde Wal-Vis-Vangst, Voorgevallen by St. Anna-Land in’t jaar 1682. den 7. October. Midsgaders, Een Pertinente Beschrijvinge, Van de geheele Groen-Landse-Vaart. Verhandeld in Prose, en Versen. (...). Door P: P: v: S. Med schoone Kop're Prentverbeeldingen vercierd. Dese 2de. Druk, merkelijk verbetert, en, bij na de helft, vermeerdert. Tot Leiden. in't Jaar 1684

    Data with "Shellfish reefs increase water storage capacity on intertidal flats over extensive spatial scales"

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    Dataset in support of the publication "Shellfish reefs increase water storage capacity on intertidal flats over extensive spatial scales" published in Ecosystems, bij Nieuwhof, Van Belzen, Oteman, van de Koppel, Herman and van der Wal. It includes the data and scripts (R and matlab) for the analyses and figures in this publication. This includes aerial images and elevation maps of the 3 shellfish reefs studied and calculated water storage capacity of the reefs. It also includes a map of water storage capacity of the Dutch Wadden Sea south of the island of Schiermonnikoog based on a bathymetry map

    Antarctic ice sheet and oceanographic response to eccentricity forcing during the early Miocene

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    Stable isotope records of benthic foraminifera from ODP Site 1264 in the southeastern Atlantic Ocean are presented which resolve the latest Oligocene to early Miocene (~24–19 Ma) climate changes at high temporal resolution (<3 kyr). Using an inverse modelling technique, we decomposed the oxygen isotope record into temperature and ice volume and found that the Antarctic ice sheet expanded episodically during the declining phase of the long-term (~400 kyr) eccentricity cycle and subsequent low short-term (~100 kyr) eccentricity cycle. The largest glaciations are separated by multiple long-term eccentricity cycles, indicating the involvement of a non-linear response mechanism. Our modelling results suggest that during the largest (Mi-1) event, Antarctic ice sheet volume expanded up to its present-day configuration. In addition, we found that distinct ~100 kyr variability occurs during the termination phases of the major Antarctic glaciations, suggesting that climate and ice-sheet response was more susceptible to short-term eccentricity forcing at these times. During two of these termination-phases, ?18O bottom water gradients in the Atlantic ceased to exist, indicating a direct link between global climate, enhanced ice-sheet instability and major oceanographic reorganisations

    The influence of the renewed supervising system of the Dutch Inspectorate of Education on teacher autonomy, protocolization and perceived educational quality

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    The Dutch Inspectorate of Education has recently (2017) launched a new supervising system to evaluate the educational quality of secondary schools in the Netherlands. In this new system, a division is made between principle-based regulation and rule-based regulation. The aim of this study was to examine 1) whether the type of regulation has an effect on teacher autonomy, 2) whether protocolization has a negative effect on teacher autonomy, 3) whether the type of regulation has an effect on protocolization, 4) whether protocolization is a mediator in the relationship between the type of regulation and teacher autonomy, and 5) whether teacher autonomy has a positive effect on perceived educational quality. Survey analyses demonstrated that a) the type of regulation has an effect on teacher autonomy, b) protocolization has a negative effect on teacher autonomy, c) the type of regulation does not have an effect on protocolization, d) protocolization is not a mediator in the relationship between the type of regulation and teacher autonomy, and e) teacher autonomy does not have an effect on perceived educational quality (although a positive effect for the principle-based regulated schools was found). Practical implications and suggestions for future research are discussed

    Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli

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    The use of carbon nanoparticles is shown for the detection and identification of different Shiga toxin-producing Escherichia coli virulence factors (vt1, vt2, eae and ehxA) and a 16S control (specific for E. coli) based on the use of lateral flow strips (nucleic acid lateral flow immunoassay, NALFIA). Prior to the detection with NALFIA, a rapid amplification method with tagged primers was applied. In the evaluation of the optimised NALFIA strips, no cross-reactivity was found for any of the antibodies used. The limit of detection was higher than for quantitative PCR (q-PCR), in most cases between 10 4 and 10 5 colony forming units/mL or 0.1-0.9 ng/¿L DNA. NALFIA strips were applied to 48 isolates from cattle faeces, and results were compared to those achieved by q-PCR. E. coli virulence factors identified by NALFIA were in very good agreement with those observed in q-PCR, showing in most cases sensitivity and specificity values of 1.0 and an almost perfect agreement between both methods (kappa coefficient larger than 0.9). The results demonstrate that the screening method developed is reliable, cost-effective and user-friendly, and that the procedure is fast as the total time required is <1 h, which includes amplification. © 2010 The Author(s).This work was partially supported by the Generalitat Valenciana (BEST/2009/026), the Universidad Politecnica de Valencia (PAID-00-09-2837), and by the Dutch Ministry of Agriculture, Nature and Food Quality (KennisBasis 6 programme). The authors would like to thank Dr. Eva Moller Nielsen at the Danish Veterinary Institute (Copenhagen, Denmark) for providing E. coli control strains and Dr. Lutz Geue (Friedrich-Loeffler-Institut, Wusterhausen, Germany) and Dr. Dorte Dopfer (School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA) for field isolates.Noguera Murray, PS.; Posthuma-Trumpie, G.; Van Tuil, M.; Van Der Wal, F.; De Boer, A.; Moers, A.; Van Amerongen, A. (2011). Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli. 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