245 research outputs found
Topical ALA-PDT with novel approach successfully treated refractory deep male urethral condyloma acuminata: A retrospective case series
No full textThese figures are supplementary to "Zhou, Zhongxia;Yang, Yutong; ;Zhang, linglin; Zhang, Haiyan; Sun, Xiaofei; Zhao, Yan; Zeng, Kang; Zhang, Guolong; Wang, Xiuli Topical ALA-PDT with novel approach successfully treated refractory deep male urethral condyloma acuminata: A retrospective case series."THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV
Characterization of the Small RNA Transcriptomes of Androgen Dependent and Independent Prostate Cancer Cell Line by Deep Sequencing
No full textGiven the important roles of miRNA in post-transcriptional regulation and its implications for cancer, characterization of miRNA facilitates us to uncover molecular mechanisms underlying the progression of androgen-independent prostate cancer (PCa). The emergence of next-generation sequencing technologies has dramatically changed the speed of all aspects of sequencing in a rapid and cost-effective fashion, which can permit an unbiased, quantitive and in-depth investigation of small RNA transcriptome. In this study, we used high-throughput Illumina sequencing to comprehensively represent the full complement of individual small RNA and to characterize miRNA expression profiles in both the androgen dependent and independent Pca cell line. At least 83 miRNAs are significantly differentially expressed, of which 41 are up-regulated and 42 are down-regulated, indicating these miRNAs may be involved in the transition of LNCaP to an androgen-independent phenotype. In addition, we have identified 43 novel miRNAs from the androgen dependent and independent PCa library and 3 of them are specific to the androgen-independent PCa. Function annotation of target genes indicated that most of these differentially expressed miRNAs tend to target genes involved in signal transduction and cell communication, epically the MAPK signaling pathway. The small RNA transcriptomes obtained in this study provide considerable insights into a better understanding of the expression and function of small RNAs in the development of androgen-independent prostate cancer
Evolutionary Mode and Functional Divergence of Vertebrate NMDA Receptor Subunit 2 Genes
No full textBackground: Ionotropic glutamate receptors in the central nervous system play a major role in numerous brain functions including learning and memory in many vertebrate species. NR2 subunits have been regarded as rate-limiting molecules in controlling the optimal N-methyl-D-aspartate (NMDA) receptor's coincidence-detection property and subsequent learning and memory function across multi-species. However, its evolutionary mode among vertebrate species remains unclear
Production, Characterization, and Antioxidant Activity of Fucoxanthin from the Marine Diatom Odontella aurita
No full textThe production, characterization, and antioxidant capacity of the carotenoid fucoxanthin from the marine diatom Odontella aurita were investigated. The results showed that low light and nitrogen-replete culture medium enhanced the biosynthesis of fucoxanthin. The maximum biomass concentration of 6.36 g L−1 and maximum fucoxanthin concentration of 18.47 mg g−1 were obtained in cultures grown in a bubble column photobioreactor (Ø 3.0 cm inner diameter), resulting in a fucoxanthin volumetric productivity of 7.96 mg L−1 day−1. A slight reduction in biomass production was observed in the scaling up of O. aurita culture in a flat plate photobioreactor, yet yielded a comparable fucoxanthin volumetric productivity. A rapid method was developed for extraction and purification of fucoxanthin. The purified fucoxanthin was identified as all-trans-fucoxanthin, which exhibited strong antioxidant properties, with the effective concentration for 50% scavenging (EC50) of 1,1-dihpenyl-2-picrylhydrazyl (DPPH) radical and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical being 0.14 and 0.03 mg mL−1, respectively. Our results suggested that O. aurita can be a natural source of fucoxanthin for human health and nutrition
MagicViewer: integrated solution for next-generation sequencing data visualization and genetic variation detection and annotation
No full textNew sequencing technologies, such as Roche 454, ABI SOLiD and Illumina, have been increasingly developed at an astounding pace with the advantages of high throughput, reduced time and cost. To satisfy the impending need for deciphering the large-scale data generated from next-generation sequencing, an integrated software MagicViewer is developed to easily visualize short read mapping, identify and annotate genetic variation based on the reference genome. MagicViewer provides a user-friendly environment in which large-scale short reads can be displayed in a zoomable interface under user-defined color scheme through an operating system-independent manner. Meanwhile, it also holds a versatile computational pipeline for genetic variation detection, filtration, annotation and visualization, providing details of search option, functional classification, subset selection, sequence association and primer design. In conclusion, MagicViewer is a sophisticated assembly visualization and genetic variation annotation tool for next-generation sequencing data, which can be widely used in a variety of sequencing-based researches, including genome re-sequencing and transcriptome studies. MagicViewer is freely available at http://bioinformatics.zj.cn/magicviewer/
Adenosine Kinase: Nutritional Regulation and Role in Diet-Induced NAFLD
No full textAdenosine is at the crossroad of transmethylation reactions and adenosine signaling. Therefore, by regulating adenosine concentration, adenosine kinase (ADK) has been identified as an upstream regulator of a complex metabolic network. However, ADK-based therapeutics has been largely limited to neural diseases, and the relationship between ADK dysfunction and non-alcoholic fatty liver disease (NAFLD) has never been investigated to date. Since the liver is the major site for methylation reactions, and ADK widely expresses throughout the body with the highest abundance in the liver, we hypothesize that hepatic ADK dysfunction might be a novel contributor to diet-induced NAFLD. By employing liver-specific ADK knockout and overexpression mouse models, in the present study, we first confirmed the upregulation of hepatic ADK in response to High-fat diet (HFD) feeding. We then proved a protective role of hepatic ADK knockout in aspects of HFD-induced NAFLD, including insulin resistance, excessive fat deposition, and low-grade metabolic inflammation in the liver. Additionally, we observed that hepatic ADK overexpression predisposed mice to NAFLD without an HFD challenge. Lastly, we touched on the mechanisms of action and determined that following upregulated ADK expression upon HFD feeding, the global DNA hypermethylation and subsequent silencing of genes of target, peroxisome proliferator-activated receptor �� (PPAR��) in specific, could be the primary mechanism underlying ADK action. Besides, the reduced adenosine signaling mediated by adenosine 2A receptor (A���AR) took a limited effect in exacerbating the aforementioned pathological process. Taken together, our research indicated the causal effects of hepatic ADK overexpression on diet-induced NAFLD. Even though more studies are warranted to further extend the mechanisms, ADK-oriented strategies hold huge promise in terms of effectively treating NAFLD
Carrier-Based Modulated Model Predictive Control for Vienna Rectifiers
No full textThe implementation of traditional finite-control-set model predictive control (FCS-MPC) with variable switching frequency in voltage source rectifiers (VSRs) can make the system suffer from poor current harmonics performance. In fact, the resulting wide-spread voltage harmonic generated at the AC terminals makes the design of the typical multi-order AC filtering bulky and prone to control instabilities. This paper proposed a fixed frequency carrier-based modulated model predictive control (CB-MMPC) which is able to overcome these issues. This control strategy aims to improve the total harmonic distortion (THD) of the AC current waveform without introducing any additional weight factor in the cost function of the optimization routine, while maintaining the typical performance of fast current dynamic response of the FCS-MPC. Herein, the detailed implementation of the proposed CB-MMPC is given, while considering its application to the current feedback control loop of a three-phase three-level Vienna rectifier. Finally, PLECS based simulation results are used to verify the feasibility and the effectiveness of the proposed control strategy and to benchmark its performance to the classical FCS-MPC strategy and the conventional application of a current closed loop implementing a proportional-integral(PI)-controller.Accepted author manuscriptDC systems, Energy conversion & Storag
Model Predictive Control for the Reduction of DC-link Current Ripple in Two-level Three-phase Voltage Source Inverters
No full textIn the applications of three-phase two-level voltage source inverters (VSIs) relatively large energy storage capacitors are used to absorb the high DC-link current ripples mainly caused by the circulating reactive power, the switched AC phase current flowing to the DC-link, and other dynamic and/or asymmetric operating conditions. Especially for electrolytic capacitor technology the typically high current stress and consequent losses is known to limit the power electronics lifetime, thus the design and selection of this component is critical for the whole system. To alleviate this problem, a new model predictive control (MPC) cost function which enables DC-link capacitor current ripple reduction is proposed in this paper. Based on the DC-link current mathematical model and the available VSI switching states, the future DC current ripple can be predicted, and then the optimized space vectors that best tracks the sinusoidal output current and minimizes the DC-link current ripple are chosen. Compared with conventional DC-link capacitor current reduction methods, the proposed approach has the advantage to incorporate an outstanding fast current control dynamics as well as being of relatively simple implementation because there is no need to adjust the switching signals or space vectors in the modulation as function of operational conditions of the system. Simulation and experimental results are presented verifying the effectiveness of the proposed MPC method.Accepted author manuscriptDC systems, Energy conversion & Storag
Characterization of In-Use Light-Duty Gasoline Vehicle Emissions by Remote Sensing in Beijing: Impact of Recent Control Measures
No full textResearch on the influence mechanism of food packaging on consumers' behavioral intention to refuse food waste based on behavioral reasoning theory
No full textGiven the escalating global concern regarding food waste, food packaging emerges as a critical area for intervention. Existing research, however, predominantly concentrates on consumer attitudes, offering limited insight into the role of food packaging in mitigating food waste. To address this gap, this study employs the BRT model, incorporating the variable of behavioral rationality into traditional theory and integrating values as a preceding variable. It integrates consumer values, behavioral rationality, attitudes, and behavioral intentions to explore the influence mechanism of food packaging on consumer behavior, explaining behavioral intentions through supporting/rejecting reasons. The study investigates how visual attributes, functionality, environmental sustainability, and social attributes within supporting reasons positively influence consumers' attitudes and behavioral intentions to reject food waste. Conversely, it investigates how information overload, functional deficiencies, inappropriate sizing, and high cognitive load within rejection reasons negatively impact these attitudes and intentions. By dissecting the positive and negative impacts of food packaging attributes on consumer attitudes and behavioral intentions across supporting and rejecting reasons, this research aims to identify key design factors for food packaging. This will fill existing research gaps, provide a theoretical foundation for food packaging design, and encourage consumers to adopt positive behaviors that reduce food waste. The findings indicate that: (1) consumer values significantly influence different behavioral attitudes; (2) supporting reasons related to food packaging positively impact the rejection of food waste behavior, while rejecting reasons have the opposite effect; (3) both positive and negative reasons significantly influence consumer attitudes and behavioral intentions, highlighting the need to optimize design elements in food packaging to positively influence consumer behavior; (4) packaging design factors have a more significant impact on intentions to conserve food than consumer attitudes themselves. This study extends the Behavioral Reasoning Theory (BRT) to understand food packaging's influence on consumer behavior, addressing contextual factors within BRT. Furthermore, it provides a scientific basis for food companies to optimize packaging design, which has practical implications for mitigating global food waste
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