1,777 research outputs found
Lateral flow serodiagnosis in the double‐antigen sandwich format: Theoretical consideration and confirmation of advantages
No full textDetermination of the presence in the blood of antibodies specific to the causative agent of a particular disease (serodiagnosis) is an effective approach in medical analytical chemistry. Serodi-agnostics performed in the lateral flow immunoassay format (immunochromatography) meet the modern requirements for point‐of‐care testing and are supported by existing technologies of large-scale diagnostic tests production, thus increasing the amount of attention in a tense epidemiological situation. For traditional lateral flow serodiagnostics formats, a large number of nonspecific immu-noglobulins in the sample significantly reduces the degree of detectable binding. To overcome these limitations, an assay based on the formation of immobilized antigen‐specific antibody‐labeled antigen complexes detection was proposed. However, the requirements for its implementation, provid-ing maximum sensitivity, have not been established. This article describes the mathematical model for the above assay. The influence of the ratio of reagent concentrations on the analysis results is considered. It is noted that the formation of specific antibody complexes with several labeled anti-gens is the main limiting factor in reducing the detection limit, and methods are proposed to minimize this factor. Recommendations for the choice of the assay conditions, following from the analysis of the model, are confirmed experimentally. © 2020 by the authors. Licensee MDPI, Basel, Switzerland
Methods and applications of in silico aptamer design and modeling
No full textAptamers are nucleic acid analogues of antibodies with high affinity to different targets, such as cells, viruses, proteins, inorganic materials, and coenzymes. Empirical approaches allow the design of in vitro aptamers that bind particularly to a target molecule with high affinity and selectivity. Theoretical methods allow significant expansion of the possibilities of aptamer design. In this study, we review theoretical and joint theoretical-experimental studies dedicated to aptamer design and modeling. We consider aptamers with different targets, such as proteins, antibiotics, organophosphates, nucleobases, amino acids, and drugs. During nucleic acid modeling and in silico design, a full set of in silico methods can be applied, such as docking, molecular dynamics (MD), and statistical analysis. The typical modeling workflow starts with structure prediction. Then, docking of target and aptamer is performed. Next, MD simulations are performed, which allows for an evaluation of the stability of aptamer/ligand complexes and determination of the binding energies with higher accuracy. Then, aptamer/ligand interactions are analyzed, and mutations of studied aptamers made. Subsequently, the whole procedure of molecular modeling can be reiterated. Thus, the interactions between aptamers and their ligands are complex and difficult to understand using only experimental approaches. Docking and MD are irreplaceable when aptamers are studied in silico. © 2020 by the authors. Licensee MDPI, Basel, Switzerland
Addendum: Urusov, A.E.; Zherdev, A.V.; Petrakova, A.V.; Sadykhov, E.G.; Koroleva, O.V.; Dzantiev, B.B. Rapid Multiple Immunoenzyme Assay of Mycotoxins. Toxins, 2015, 7, 238-254.
No full textThe authors would like to replace the Acknowledgements section with the following: [...
The Life and Vegetating
No full textFor several years, A.V. Suvorov has been doing mailing lists for like-minded colleagues and friends. Each of the letters is a scientific and journalistic text on philosophical, psychological, pedagogical, and life topics. A.V. Suvorov signed his letters "Hedgehog" - that's how he called himself, meaning not only prickly, but also the desire to isolate himself for a while, shrink, withdraw into himself from the world, so that he could then turn to him with something important. We bring to your attention his latest essay, or rather, the last revision of the text that he wrote almost a quarter of a century ago. The author reflects on how to overcome universal, collective and individual, personal nonsense as a way of existence. In his opinion, didactics is intended to provide a means of understanding existence.</p
Teaching foreign students argumentation on the example of A.V. Vampilov’s “The elder son”
No full textThe article concerns ways of teaching argumentation to foreign students learning Russian as a foreign language. The author characterizes extracts of A.V. Vampilov's “The Elder Son” where different verbal persuasive means showing menthality, specifiс way of thinking and world perception of the Russians are presented
Large-eddy simulation of turbulent channel flow using the explicit algebraic subgrid-scale model
No full textLarge-eddy simulation (LES) of turbulent channel flow are performed with a new subgrid-scale (SGS) stress model. The simulations show that with this model we can well predict turbulent wall flows at coarse resolutions and moderately high Reynolds numbers. The commonly used dynamic Smagorinsky model fails at coarser resolutions
Explicit algebraic and differential Reynolds stress model application to homogeneously sheared and compressed turbulence
No full textAn explicit algebraic and differential Reynolds stress models (EARSM and DRSM) are used to investigate the influence of homogeneous shear and compression on the behaviour of turbulence in the limit of rapid distortion theory (RDT). EARSM is shown to give realizable results and to preserve RDT regime, unlike the eddy-viscosity model (EVM). The DRSM version of our model is in reasonable agreement with RDT theory
Generalissimo A.V. Suvorov: General’s Persona in Memory Politics of Early 20th Century Russian Empire
Get PDFThis article addresses the utilization of heroic imagery from the past within the memory politics of the early 20th century Russian Empire. The author provides a detailed analysis of the commemoration of A.V. Suvorov’s feats during the reign of Nicholas II. It is demonstrated that the centenary of Suvorov’s death catalyzed a resurgence of interest in this heroic figure. Based on archival documents and periodical press, the study shows that efforts to immortalize the general continued after the centennial anniversary. The research establishes that Victorian themes occupied a central position in the symbolic politics of autocratic power throughout this period. The author concludes that the state actively employed the heroic image of A.V. Suvorov to legitimize the existing order and unify the empire’s populace. It is emphasized that, in the early 20th century, key instruments for shaping the memory landscape included the construction of a pantheon of national heroes, the establishment of museums, the organization of celebratory ceremonies, and the execution of educational initiatives, alongside monumental commemoration. The author asserts that narratives from military history were instrumental in creating cultural infrastructure both within and beyond the state’s borders. This article is of particular interest to researchers exploring issues related to historical memory and memory politics in the early 20th century
Lutein and zeaxanthin complex: ophthalmologists’ choice
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A.V. Korneeva
LLC “Three-Z-Moscow”, Moscow, Russian Federation
This article summarizes the data on carotenoids and their role in retinal antioxidant protection. The relevance of lutein and zeaxanthin complexes and criteria for their choice are discussed. Data on the relationship between low carotenoid levels in the fovea and age-related macular degeneration (AMD) are available. There are several lutein and zeaxanthin complexes which formulations were developed according to the AREDS2. Clinical trials demonstrate that lutein and zeaxanthin complexes improve both retinal functional activity and morphological parameters and are effective to prevent AMD development and progression. They are recommended for unbalanced diet, gastrointestinal disorders, psychoemotional stresses (e.g., drivers or professional athletes), intensive ultraviolet (e.g., welders, lighters or mountain tourists), myopia, accommodation cramp, inflammatory and degenerative ocular disorders. Optimal dosing is 1 tablet per day for at least 3 months twice a year.
Key words: lutein, zeaxanthin, carotenoids, antioxidants, age-related macular degeneration.
For citation: Korneeva A.V. Lutein and zeaxanthin complex: ophthalmologists’ choice. Russian Journal of Clinical Ophthalmology. 2019;19(1):54–58.
About the author:
Alina V. Korneeva — MD, PhD, ophthalmologist.
“Three-Z-Moscow”. 3, Boris Galushkin Str., Moscow, 129301, Russian Federation.
Contact information: Alina V. Korneeva, e-mail: [email protected]. Financial Disclosure: author has no a financial or property interest in any material or method mentioned. There is no conflict of interests. Received 17.10.2018.<br
Author Correction:A 41,500 year-old decorated ivory pendant from Stajnia Cave (Poland)
No full textCorrection to: Scientific Reports https://doi.org/10.1038/s41598-021-01221-6, published online 25 November 2021The original version of this Article contained errors in the author list where Marjolein D. Bosch was omitted from the author list, and Mikołaj Urbanowski was incorrectly listed as an author of the original Article, and has subsequently been removed.The Author contributions section now reads:“S.T. W.N. and A.N. conceived the project; S.T., W.N., A.P., M.B., S.C., M.D., H.F., A.M., M.D. B., D.P., M.P.R., C.M.R., V.S-M., G.M.S., P.S., M.S., K.S., A.V., F.W., H.W., A.W., M.Z., S.B., A.N., J-J. H., performed research; S.T., A.P., W.N., M.B., M.D.B., S.C., M.D., H.F., A.M., D.P., M.P.R., C.M.R., V.S-M., G.M.S., P.S., M.S., K.S., A.V., F.W., H.W., A.W., M.Z., S.B., A.N., J-J. H. analysed all archaeological data; S.T. and A.P. wrote the paper with the collaboration of all the co-authors.”The original Article and its accompanying Supplementary Information file have been corrected
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