1,721,027 research outputs found
Attivazione dei recettori per formil-peptidi e regolazione della NADPH ossidasi in lineee cellulari tumorali umane non fagocitiche
No full textL’obiettivo del presente progetto è quello di identificare, in linee cellulari tumorali stimolate con ligandi per i recettori dei formil-peptidi, i pathway di segnale coinvolti nella fosforilazione di p47phox e quindi del controllo della NADPH ossidasi non fagocitica, nonchè la cascata di segnalazione capace di promuovere la proliferazione cellulare.
In particolare ci proponiamo di: i) identificare il recettore per formil-peptidi e le componenti della ossidasi espressi nelle cellule HCT-116 (carcinoma umano del colon), HepG2 (epatoma umano), Saos-2 (osteosarcoma umano) e Calu-6 (carcinoma anaplastico umano del polmone), ii) valutare il ruolo dell’espressione e dell’attivazione di Nox, dopo stimolazione con gli opportuni ligandi, nella patogenesi dei tumori; iii) studiare il pathway di segnalazione intracellulare innescato dal legame agonista/recettore (FPR e/o FPRL1) che conduce all’attivazione di Nox e responsabile della proliferazione cellulare
Suicidio tra umanesimo e scienza
No full textSuicide is among the first 10 causes of death in industrialized countries,
even if its incidence is declining. Familiarity frequently occurs in suicide and a myriad of polymorphisms in candidate
genes has been studied as risk factors. Among these, genes encoding enzymes, transporters or receptors of the
serotoninergic and dopaminergic systems have been widely studied with conflicting results. Also the metabolism of
cholesterol (and serum cholesterol concentrations) seems to have a role in the pathogenesis of suicide. In the last
years, our group contributed to study brain derived neurotrophic factor (BDNF) and its TrkB receptor genes in the
DNA from Wernicke area from a large cohort of suicide subjects and controls. In particular, we excluded that the
expression of BDNF and its receptor may be modulated by gene mutations. However, the levels of BDNF gene
expression were significantly lower in the brain tissue from suicide subjects. We demonstrated that the altered BDNF
expression was due to the enhanced methylation of BDNF promoter. These studies first revealed the association
between epigenetics and suicide suggesting a novel model of interaction between environment and genes in the pathogenesis of suicide
Identificazionee caratterizzazione di mutazioni in regioni regolatorie del gene malattia della fibrosi cistica
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Genetic Diseases That Predispose to Early Liver Cirrhosis
Full text linkInherited liver diseases are a group of metabolic and genetic defects that typically cause early chronic liver involvement. Most are due to a defect of an enzyme/transport protein that alters a metabolic pathway and exerts a pathogenic role mainly in the liver. The prevalence is variable, but most are rare pathologies. We review the pathophysiology of such diseases and the diagnostic contribution of laboratory tests, focusing on the role of molecular genetics. In fact, thanks to recent advances in genetics, molecular analysis permits early and specific diagnosis for most disorders and helps to reduce the invasive approach of liver biopsy
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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