1,720,984 research outputs found
Elettroferotipi di rotavirus umani riscontrati in neonati e bambini a Parma da Gennaio 1987 a Giugno 1994
No full textValutazione di alcuni aspetti della nuova tecnologia VITEK2 effettuata presso gli Istituti di Microbiologia dell'Università di Parma e dell'Università Cattolica di Roma. Workshop: VITEK2: un mondo d'innovazione.
No full textRIWB in comparison with conventional WB, second and third generation ELISA assays for the serodiagnosis of HIV infection
No full textStudio epidemiologico di rotavirus umani responsabili di enterite acuta infantile a Parma negli anni 1987-1994. Caratterizzazione elettroferotipica dei ceppi virali
No full textEight year study of rotavirus electropheotypes from cases of infantile gastroenteritis in Parma, Italy
No full textRadioimmune western blotting in comparison with conventional western blotting, second and third generation ELISA assays for the serodiagnosis of HIV-1 infection.
No full textAbstract
We compared the performance of second and third generation ELISA assays to detect antibodies to HIV-1 virus with conventional Western blotting (WB) and radioimmune Western blotting (RIWB). Both sera from commercial seroconversion panels and serial dilutions of a serum for HIV-1 antibodies were tested with Murex HIV Recombinant, Vidas bioMérieux HIV 1/2 (2nd generation ELISA) Murex HIV 1-2 (3rd generation ELISA), as well as with WB and RIWB. In seroconversion panels all ELISA assays were positive for the same serum with the exception of the first serum of Panel D which was negative with both sample Murex assays and borderline with Vidas assay. This serum was negative with WB but evidenced antibodies to gp160 p66, p51, p24 HIV-1 proteins when assayed by RIWB. In only two cases did WB reveal antibodies to HIV-1 proteins before ELISA assays (Panel A and E); not only did RIWB show the same sensitivity as WB in the two last panels, but it also detected antibodies to HIV-1 proteins earlier than WB, ranging from a few days (Panel C) to approximately 12 weeks (Panel D). The results obtained by testing the dilutions of the serum positive for anti HIV-1 antibodies showed the following degrees of sensitivity: Murex HIV 1-2 (the most sensitive), Murex HIV Recombinant and Vidas bioMérieux HIV 1/2. Although WB was more sensitive than the ELISA assays and picked out antibodies to gp160, gp120 and p24 HIV proteins at 1/4000 serum dilution, the most sensitive test was RIWB which at 1/20,000 serum dilution enabled detection of antibodies to gp160, p66 and p24 HIV proteins.
PMID: 7968651 [PubMed - indexed for MEDLINE
La reazione polimerasica a catena: studio preliminare di valutazione per un suo impiego nella diagnosi di infezione da citomegalovirus
No full textPrevalence of antibodies to HIV-1 and HIV-2 in women attending a sexually transmitted disease clinic in Luanda, Angola
No full text
La diagnosi di laboratorio di infezione da citomegalovirus attraverso l’impiego di “nested PCR”. Risultati della valutazione di un saggio commerciale
No full text- …
