35 research outputs found
Yulia Rosa Saharman's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
Yulia Rosa Saharman's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
Yulia Rosa Saharman's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
Analysis of Klebsiella Pneumonia and Resistance Genes Coding and Carbapenemase Production in National Referral Hospital Wastewater
No full textHospitals, as a source of transmission and spread of bacteria carrying antibiotic-resistant genes (ARGs) as well as a high source of antibiotic compounds, are a significant reservoir of antibiotic-resistant gene transfer that causes bacteria to develop multidrug resistance (MDR). Klebsiella pneumonia is a bacterium often found in wastewater with a high level of Extended Spectrum β-lactamase (ESBL) and carbapenemase resistance. This study aimed to determine Klebsiella pneumonia and resistance genes encoding ESBL and carbapenemase in hospital wastewater to obtain primary Antimicrobial Resistance (AMR) data in the environment, which was first conducted in Indonesia. The resistance gene detection method was developed using High Throughput Real-Time and conventional Real-Time (HT-RT PCR). The data obtained were relative abundance and copy number. The results showed that blaTEM and blaNDM were the highest ARGs. Conventional Real-Time PCR results showed better sensitivity in detecting K. pneumoniae and ARGs than HT-RT PCR. The detection of K. pneumoniae and ARGs in RSCM wastewater in Indonesia indicates the need to improve the handling of RSCM WWTP to monitor the number of microbial resistances to antibiotics
Mobile Genetic Elements Contributing to Carbapenem Resistance in Acinetobacter baumannii: Current Insights
Get PDFAcinetobacter baumannii has become a major cause of hospital-acquired infections with the rapid development of resistance to multiple antibiotics, including critical carbapenems. This resistance challenge limits treatment options and increases morbidity and mortality. The genetic plasticity of A. baumannii facilitates the mobilization of resistance genes via mobile genetic elements (MGE). Addressing this crisis requires a deeper understanding of the mechanisms by which MGE propagates carbapenem resistance. This paper provides a solution by systematically reviewing recent research on the role of MGE in disseminating resistance genes. Following PRISMA guidelines, a comprehensive literature review was conducted across various databases. The review revealed that resistance mechanisms primarily involve carbapenem-hydrolyzing enzymes and MGE, such as integrons, transposons, insertion sequences, and plasmids. Notably, genes like blaOXA-23 and blaNDM are frequently mobilized by these elements, facilitating horizontal gene transfer and persistence. Understanding the mechanisms of MGE-mediated gene transfer is crucial for developing strategies to control the spread of antibiotic resistance in A. baumannii
Multidrug-Resistant Bacteria Colonization in Patients Admitted to Dr. Cipto Mangunkusumo Hospital Jakarta, Indonesia
Get PDFBackground: Antibiotic resistance is the main problem in infectious disease management. Multidrug-resistant (MDR) bacteria could be carried by admitted patients and become a source of spread in the hospital, causing infections in other patients or the patients themselves. However, the screening of MDR bacteria has not been a standard in developing countries. This study aimed to get the prevalence of MDR bacteria colonization in patients on admission to Dr. Cipto Mangunkusumo Hospital. Methods: Selective liquid media with added antibiotics were used for culturing the MDR bacteria. While admitted to the hospital, subjects were sampled and interviewed to fill out a questionnaire. The screening specimens used for this study were throat, navel, rectal, nasal, and armpit swabs. During hospitalization, hospital-acquired infections (HAIs) were recorded. Results: Of 100 patients included in the study, the prevalence of MDR bacteria colonization on admission was 63% (n=63) with the prevalence of CR-GNB, ESBL-PE, and MRSA were 11%, 54%, and 11%, respectively. Two-thirds of the patients with HAIs (n=8/12) were colonized with MDR bacteria. Factors associated with MDR bacteria colonization were the recent use of invasive medical devices and comorbidity, while a factor associated with CR-GNB colonization was the recent use of antibiotics. Conclusion: The prevalence of MDR bacteria colonization in patients on admission to Dr. Cipto Mangunkusumo Hospital in 2022 was 63% (n=63), of which 12.68% (n=8) experienced HAIs during hospitalization. MDR bacteria colonization was associated with the recent use of invasive medical devices and comorbidity. History of antibiotic use was associated with CR-GNB colonization
Optimizing Gram-Negative Bacteria Isolation from Children Diarrheal Stool Samples in Jakarta: A Comparative Analysis of Six Culture Media
Get PDFThe prevalence of diarrheal illnesses in Indonesia remains a significant concern among children under the age of five, evident through diagnostic findings and symptomatic manifestations, leading to increased rates of mortality and morbidity. This study aimed to identify, isolate, and culture gram-negative bacteria responsible for childhood diarrhea. Twenty stool samples were collected from children under the age of 5 with acute diarrhea in Jakarta health centres and hospitals between October 2023 to January 2024. These samples were inoculated onto selective agars to facilitate the growth of gram-negative bacteria. After a 24-hour incubation period, colonies suspected to be E. coli, K. pneumoniae, Salmonella, Shigella, or Vibrio were identified using the Vitek-2 compact system. The results revealed a notable presence of Gram-negative bacteria in all the fecal samples from diarrheal children, with Escherichia coli (E. Coli) and Klebsiella pneumoniae (Klebsiella p) as the predominant species. In conclusion, our study, utilizing various cultures and the Vitek-2 compact system, elucidates the microbial landscape driving diarrheal morbidity in Indonesian children under five, with E. Coli and klebsiella pneumoniae emerging as prevalent contributors to childhood diarrhea in this population
Epidemiology and Management of Multi Drug Resistance Bacterial Pathogens in Adult Intensive Care Units in Lower Middle-Income Countries
No full textEpidemiology and Management of Multi Drug Resistance Bacterial Pathogens in Adult Intensive Care Units in Lower Middle-Income Countries
No full textEpidemiology and Management of Multi Drug Resistance Bacterial Pathogens in Adult Intensive Care Units in Lower Middle-Income Countries
No full text
