52 research outputs found
Argon adsorption on MCM-41 mesoporous crystal studied by in situ synchrotron powder X-ray diffraction
No full textEquilibrium argon adsorption from the gas phase on mesoporous MCM-41 silica of hexagonal structure is directly studied by in situ synchrotron powder X-ray diffraction (XRD) measurements at SPring-8. The diffraction intensity data is analyzed by extending the previously developed analytical formula for the crystal structure factors of MCM-41 to account for argon adsorbed in the pores. It is clearly observed that argon adsorbs in layers on the pore walls at low gas pressures and exhibits sudden capillary condensation as the gas pressure increased. The proposed method of interpretation of XRD data allows one to calculate the density ratio between the silica wall and condensed argon, the pore size, and the pore wall fluctuation/roughness, together with the thickness of the adsorbed layer as a function of the gas pressure. The results of in situ XRD experiments are compared with the results of argon adsorption volumetric experiments. The adsorption data are interpreted with the quench solid density functional theory (QSDFT), which takes into account the pore wall roughness. The perfect agreement of the QSDFT isotherm predicted from the adsorption data and the XRD recalculated isotherm suggests that the adsorption porosimetry and XRD measurements can be reconciled provided a proper interpretation of the experimental data.</p
Genome annotation of Hibiscus trionum
No full textGenome annotation of Hibiscus trionum
Author information
Shizuka Koshimizu1,2, Sachiko Masuda3, Arisa Shibata3, Takayoshi Ishii4, Ken Shirasu3, Atsushi Hoshino5,6, Masanori Arita1,2
1 Bioinformation and DDBJ Center, National Institute of Genetics, Mishima 411-8540, Japan
2 Graduate Institute for Advanced Studies, SOKENDAI, Mishima 411-8540, Japan
3 Center for Sustainable Resource Science, RIKEN, Yokohama 230-0045, Japan
4 Arid Land Research Center, Tottori University, Tottori, 680-001, Japan
5 National Institute for Basic Biology, Okazaki 444-8585, Japan
6 Graduate Institute for Advanced Studies, SOKENDAI, Okazaki 444-8585, Japan
Contents
htrionum_v1.0_genome.fasta: Genome sequence
htrionum_v1.0_transcripts.gff3: Gene annotation
htrionum_v1.0_transcripts.gtf: Gene annotation
htrionum_v1.0_transcripts.fna: Transcript sequences
htrionum_v1.0_pep.faa: Protein sequences
Description.txt: Functional descriptions
InterProScan.txt: The results of InterProScan for all proteins
Reference
Genome and transcriptome analyses reveal genes involved in the formation of fine ridges on petal epidermal cells in Hibiscus trionum
DNA Research, 2023;, dsad019, https://doi.org/10.1093/dnares/dsad01
Automated Ribosomal Intergenic Spacer Analysis to Detect Pathogenic Fungi in Dark-Colored Spots on Apple Fruits
No full textDark-colored spots (DCSs) found on apples at harvest time worldwide significantly reduce the commercial value of the fruits. In this study, we conducted an automated ribosomal intergenic spacer analysis (ARISA) to reliably detect fungal pathogens in these lesions. The fragment sizes of peaks for eight species and two species complexes that might cause DCSs on apples in northern Honshu Island, Japan, were determined on the basis of the ARISA. The fragment sizes for six species (Botryosphaeria kuwatsukai, Diplocarpon mali, Mycosphaerella pomi, Neonectria galligena, Phlyctema vagabunda, and Venturia inaequalis) were unique, enabling their detection in a single ARISA trial. However, two other species and two species complexes had similar fragment sizes. To identify these fungi, PCR amplifications using species- or genus-specific primers were needed. Using this method to examine the fresh tissue of DCSs (i.e., after removing the epidermis) on commercially produced apples resulted in the detection of six fungal species and two Colletotrichum species complexes. Because the pathogenic fungi in the tissue underlying lesions are most likely the causal agents, these fungi could be responsible for the DCSs on apple fruits. An automated analysis of the PCR products amplified for the ARISA enables the rapid examination of many samples. These results indicate that the ARISA-based technique developed in the present study is useful for the accurate, reliable, and comprehensive detection of the pathogens causing DCSs on apple fruits. [Figure: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license
Applying the FAO Surveillance Evaluation Tool (SET) to assess the fish farming disease surveillance system in Spain
No full textEU Member States should ensure that they implement adequate health surveillance schemes in all aquaculture farming areas, as appropriate for the type of production. This study presents the results of applying the FAO's Surveillance Evaluation Tool (SET) to assess the Spanish disease surveillance system for farmed fish species, which although applied previously in livestock production, is applied here to aquaculture for the first time. Overall, there were important score differences between trout and marine fish (seabass and seabream) surveillance, which were higher for trout in the following areas: Institutional (70.8% versus 50.0%), Laboratory (91.7% versus 47.2%), and Surveillance activities (75.3% versus 61.3%). For other categories, the values were lower and no significant differences were found. However, most surveillance efforts focused only on trout, for which there are EU and WOAH listed (notifiable) diseases. In contrast, for seabream and seabass, for which there are no listed diseases, it was considered that surveillance efforts should, nevertheless, be in place and should focus on the identification of abnormal mortalities and emerging diseases, for which there are as yet no standardized harmonised methodologies.por la que se aprueban las normas reguladoras para la concesión de ayudas a las Agrupaciones de DefensaThe author(s) declare financial support was received for the research, authorship, and/or publication of this article. BB reports financial support was provided by Horizon 2020. This study received funding from the European Commission Horizon 2020 (H2020) Framework Programme through grant agreement no 727315 MedAID project (Mediterranean Aquaculture Integrated Development), as well as from the Project ARISA, funded by the Biodiversity Foundation, of the Ministry for Ecological Transition and Demographic Challenge, through the Pleamar Programme, co-financed by the European Maritime and Fisheries Fund 2014–2020.info:eu-repo/semantics/publishedVersio
Study on Physicochemical Properties and its Effective Use of Asphalt Pavement Cutting Waste Water
No full textAbstractWhile cutting asphalt pavement surfaces, cooling water is continuously given to the cutting machine blades to prevent the heat increase and to control the scatter of cutting dusts. As the results, asphalt pavement cutting waste water is generated. The amount of waste water generated at one construction site is by no means a lot, however, the water quality is not environmentally friendly. In view of above, the physical and chemical properties of cutting waste water were first investigated. Then the possibility of effective use of the cutting waste water was investigated using flocculants and granulation technique. The results show that the waste water quality can be improved by the effects of flocculants. Mud sediments after flocculated in the waste water can be granulated and the granules may be used as recycled sands or recycle crusher-runs aggregates
Total synthesis and biological activity of dolastatin 16
No full textThe total synthesis of dolastatin 16, a macrocyclic depsipeptide first isolated from the sea hare Dolabella auricularia as a potential antineoplastic metabolite by Pettit et al., was achieved in a convergent manner. Dolastatin 16 was reported by Tan to exhibit strong antifouling activity, and thus shows promise for inhibiting the attachment of marine benthic organisms such as Amphibalanus amphitrite to ships and submerged artificial structures. Therefore, dolastatin 16 is a potential compound for a new, environmentally friendly antifouling material to replace banned tributyltin-based antifouling paints. The synthesis of dolastatin 16 involved the use of prolinol to prevent formation of a diketopiperazine composed of L-proline and N-methyl-D-valine during peptide coupling. This strategy for the elongation of peptide chains allowed the efficient and scalable synthesis of one segment, which was subsequently coupled with a second segment and cyclized to form the macrocyclic framework of dolastatin 16. The synthetic dolastatin 16 exhibited potent antifouling activity similar to that of natural dolastatin 16 toward cypris larvae of Amphibalanus amphitrite
Major Willie Redmond and Irish Home Rule
No full textAfter the 1880s, the Irish Nationalist Party demanded Irish Home Rule. In 1914, the Third Home Rule Bill was passed in the House of Commons. It then only awaited Royal Assent, but soon after the outbreak of the Great War, the British government decided to postpone the enactment of Irish Home Rule. Willie Redmond, MP, the brother of John Redmond, enlisted for the Irish Division in the British Expeditionary Forces and was killed in the Battle of Messines in 1917. Willie’s death caused a vacancy in the East Clare constituency and the Sinn Fein candidate, Eamon de Valera, won the seat. This was a significant turning point in Irish Nationalism from Home Rule to Republicanism. This article will examine Willie Redmond’s motives and discuss the cause of Irish Home Rule in the context of the Great War. To distinguish from John Redmond, MP, Willie’s elder brother, and William Archer Redmond, MP, Willie’s nephew, the author will use the popular name of Willie for William Redmond in this article
Major Willie Redmond and Irish Home Rule
No full textAfter the 1880s, the Irish Nationalist Party demanded Irish Home Rule. In 1914, the Third Home Rule Bill was passed in the House of Commons. It then only awaited Royal Assent, but soon after the outbreak of the Great War, the British government decided to postpone the enactment of Irish Home Rule. Willie Redmond, MP, the brother of John Redmond, enlisted for the Irish Division
in the British Expeditionary Forces and was killed in the Battle of Messines in 1917. Willie’s death caused a vacancy in the East Clare constituency and the Sinn Fein candidate, Eamon de Valera, won the seat. This was a significant turning point in Irish Nationalism from Home Rule to Republicanism. This article will examine Willie Redmond’s motives and discuss the cause of Irish Home Rule in the context of the Great War. To distinguish from John Redmond, MP, Willie’s elder brother, and William Archer Redmond, MP, Willie’s nephew, the author will use the popular name of Willie for William Redmond in this
article
Sulfurifustis variabilis gen. nov., sp nov., a sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov and Acidiferrobacterales ord. nov.
No full textA novel autotrophic bacterium, strain skN76(T), was isolated from sediment of a lake in Japan. As sole electron donor to support chemolithoautotrophic growth, the strain oxidized thiosulfate, tetrathionate and elemental sulfur. For growth, the optimum temperature was 42-45 degrees C and the optimum pH was 6.8-8.2. The cells were Gram-stain-negative, catalase-positive and oxidase-positive. The strain exhibited changes in morphology depending on growth temperature. Cells grown at the optimum temperature were rod-shaped (0.9-3.0 mu m long and 0.3-0.5 mu m wide), whereas a filamentous form was observed when the strain was cultured at the lowest permissive growth temperatures. The G+C content of genomic DNA was 69 mol%. The major components in the fatty acid profile were C-16 : 0, summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) and summed feature 9 (iso-C-17 : 1 omega 9c and/or 10-methyl C-16 : 0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest cultivated relative of strain skN76(T) was Acidiferrobacter thiooxydans m-1(T), with sequence similarity of 93 %. On the basis of its phylogenetic and phenotypic properties, strain skN76(T) (=DSM 100313(T)= NBRC 110942(T)) is proposed as the type strain of a novel species of a novel genus, Sulfurifustis variabilis gen. nov., sp. nov. Novel taxa, Acidiferrobacteraceae fam. nov. and Acidiferrobacterales ord. nov., are also proposed to accommodate the genera Acidiferrobacter and Sulfurifustis gen. nov
Discursive othering of Asian Americans: A preliminary reflection of a foreshadowing COVID-19’ related hate
No full textThis study aims to examine the underlying causes of the hate against Asian Americans in relation to coronavirus disease 2019 (COVID-19). Using discourse analysis, the author analyzes interview data collected before the outbreak of the pandemic in the Bay Area of the United States. Anti-Asian sentiment did not start after the beginning of the outbreak. Rather, it has existed in American society, and people in the Asian community have faced prejudice and hate incidents before the outbreak as well. This study examines the discourse in two ways: discourse as practices and discourse as language in use. Through the analysis of interview data, this study aims to 1) shed light on Asian Americans’ experiences and reveal the racism Asians are subjected to; 2) examine how the discourse affects the identity and situation of Asians under a certain dominance; 3) analyze how discourse reproduces systemic violence against Asian Americans. The analysis shows that the same discourse which has positioned Asians as the “other” is reproduced both socially and politically and that is a reason for violence against Asian Americans. However, it is further revealed that Asian people construct their identity through these discourses, by either internalizing or opposing them
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