4,647 research outputs found

    Screening and Analysis of Antifungal Strains Bacillus subtilis JF-4 and B. amylum JF-5 for the Biological Control of Fusarium Wilt of Banana

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    Purpose: This study aimed to identify the antagonistic bacteria from the rhizosphere of healthy bananas that can effectively suppress the Fusarium wilt of banana, and to further investigate the inhibitory mechanism. Method: The primary and secondary screening techniques were implemented using the double-plate and fermentation antagonism methods. The strain was identified based on physiological and biochemical tests, 16S rRNA gene sequencing, and specific gene amplification. The effects of crude extract on the protein content, lipid peroxidation, and pectinase activity of mycelia were determined from the identified isolates. Results: Two antagonistic bacteria, JF-4 and JF-5, were screened and initially identified as Bacillus subtilis (GenBank: OR125631) and B. amylum (GenBank: OR125632). The greenhouse experiment showed that the biological control efficiency of the two antagonists against the Fusarium wilt of banana was 48.3% and 40.3%, respectively. The catalase content produced by lipid peroxidation increased significantly after treatment with the crude extracts of JF-4 and JF-5 at concentrations of 0.69 μmol/L and 0.59 μmol/L, respectively. The protein and ergosterol content and pectinase activity decreased significantly. The two antagonistic bacteria might inhibit the growth of pathogens by enhancing lipid peroxidation and decreasing the synthesis of cell metabolites. Twenty compounds were identified by gas chromatography–mass spectrometry (GC-MS). B. subtilis JF-4 was further sequenced and assembled to obtain a complete circular chromosome genome of 681,804,824 bp. The genome consisted of a 4,310,825-bp-long scaffold. Conclusion: The findings of this study may help elucidate the mechanism behind this biocontrol isolate

    Nonreciprocal ground-state cooling of multiple mechanical resonators

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    The simultaneous ground-state cooling of multiple degenerate or near-degenerate mechanical modes coupled to a common cavity-field mode has become an outstanding challenge in cavity optomechanics. This is because the dark modes formed by these mechanical modes decouple from the cavity mode and prevent extracting energy from the dark modes through the cooling channel of the cavity mode. Here we propose a universal and reliable dark-mode-breaking method to realize the simultaneous ground-state cooling of two degenerate or nondegenerate mechanical modes by introducing a phase-dependent phonon-exchange interaction, which is used to form a loop-coupled configuration.We find an asymmetrical cooling performance for the two mechanical modes and expound this phenomenon based on the nonreciprocal energy transfer mechanism, which leads to the directional flow of phonons between the two mechanical modes.We also generalize this method to cool multiple mechanical modes. The physical mechanism in this cooling scheme has general validity and this method can be extended to break other dark-mode and dark-state effects in physics

    Business Model Innovation of JF Logistics Company

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    摘要 随着全球化经济的发展,市场竞争变得越来越复杂。信息时代使得物流供应链管理已上升到企业的战略管理高度。在这样的背景下,本文应用翁君奕老师的介观商务模式创新观点,对JF物流公司所处行业现状进行剖析,重新审视了外部客户市场以及内部自身情况,找出了JF物流公司自身的优势,并结合外部市场客户的需求,提出了“为客户提供个性化的集物流、资金流、信息流于一体的供应链物流服务”这一价值主张,并在此基础上,重新定位客户市场,创新服务产品,理顺内部管理架构和业务流程以支撑和保持这一价值主张。文中同时以例证来说明依据新价值主张所创新的服务产品给JF物流公司所带来的变化,以此说明通过商务模式创新来实行自身的战略...Abstract With the development of the global economy, the competition in market becomes more complicated. In the era of information, logistics and supply chain management is regarded as important as part of the company strategy. Under such background , the author of this essay uses the concept of “JieGuan Business Model Innovation” proposed by Professor Weng Junyi of Xiamen University, and analy...学位:管理学硕士院系专业:管理学院高级经理教育中心(EMBA项目)_管理经济学学号:X200615614

    Temporal and spatial variability in speakers with Parkinson's Disease and Friedreich's Ataxia

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    Speech variability in groups of speakers with Parkinson's disease (PD) and with Friedreich's ataxia was compared with healthy controls. Speakers repeated the same phrase 20 times at one of two rates (fast or habitual). A non-linear analysis of variability was performed which used some of the principles behind the spatio-temporal index (STI). The STI usually employs variation in lip displacement over repetitions of the same utterance and a linear analysis of such signals is conducted to represent the combined variation in spatial and temporal control. When working with patients, audio measures (here we used speech energy) are preferred over kinematics ones as they are minimally disruptive to speech. Non-linear methods allow spatial variability to be estimated separately from temporal variability. The results are tentatively interpreted as showing that PD speakers were distinguished from healthy control speakers in spatial variability and ataxic speakers were distinguished from controls in temporal variability. These findings are consistent with the speech symptoms reported for these disorders. We conclude that the non-linear analysis using the speech energy measure is worth investigating further as it is potentially revealing of the differences underlying these two pathologies

    Chip-Level Channel Estimation for the Downlink of a WCDMA System in Very High Mobility Environment 

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    [[abstract]]This article proposes a channel estimation method for the downlink channels of a WCDMA system in a high-speed railroad setting. High mobility may cause conventional symbol-level channel estimation to yield severe errors because in conventional methods channel state has to maintain constant within one to several symbol durations. However. in high mobility environment, this assumption may not hold. Errors are particularly more dangerous when using very high spreading factors. In order to counteract the adverse effect of high mobility on channel estimation, we shorten the observation window to that of an N-chip block so that channel conditions or characteristics remain approximately unchanged. We consider channel estimation prior to dispreading the received signal. In other words, channel estimation is done Lit the chip level rather than the conventional symbol level. The least squares (LS) criterion is employed to acquire channel characteristics for each block of N pilot chips, and the linear interpolation method is used to determine the channel characteristics for each data chip. The LS-based estimator is selected due to its simplicity since it does not need to know channel or noise statistics. An LS-based estimator at the chip level has the further advantage that it is robust against interpath interference (IPI). The uncoded bit error rate (BER) performance of a RAKE receiver using different channel estimation schemes is evaluated and compared through simulations. The proposed scheme is found to be suitable for a high-speed railroad setting.[[note]]SC

    Ethanol ablation of hepatocellular carcinoma Up to 5.0 cm by using a multipronged injection needle with high-dose strategy

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    PURPOSE: To investigate whether ethanol ablation by using a multipronged needle delivery system (multipronged ethanol ablation) could eradicate hepatocellular carcinoma (HCC) up to 5.0 cm in diameter with a single-session high-dose strategy. MATERIALS AND METHODS: The hospital ethics committee approved the prospective study, and each patient provided written informed consent. One hundred forty-one patients (125 men, 16 women; mean age, 53 years; range, 27-76 years) with 164 primary or recurrent HCC ranging from 1.3 to 5.0 cm in diameter (mean, 2.9 cm +/- 0.9) were treated with high-dose multipronged ethanol ablation. Patients were unsuitable for surgery, declined surgery and radiofrequency ablation, or had tumors located at unfavorable sites. Primary technique effectiveness (PTE) (complete ablation within two sessions), local tumor progression (LTP), and complications after the treatment were observed. Twenty risk factors of local effectiveness and complications were analyzed by means of univariate and multivariate analysis. RESULTS: Mean number of treatment sessions was 1.1. The mean volume of ethanol per tumor was 31 mL (range, 8-68 mL). PTE was achieved in 134 (95%) of 141 patients and was significantly associated with tumor pattern (capsulated vs noncapsulated, P = .018). After a mean follow-up period of 25 months, LTP was observed in 16 (12%) of 134 patients, and in nine (56%) patients, LTP occurred in tumors 3.1-5.0 cm in diameter. Alanine aminotransferase level (P = .023) was the independent risk factor for LTP. Three (2%) of 141 patients had major complications. CONCLUSION: Multipronged ethanol ablation with a high-dose strategy can be used to treat HCC up to 5.0 cm in diameter effectively and safely, often in a single sessio

    Additional file 6 of Prevalence of chronic cough in China: a systematic review and meta-analysis

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    Additional file 6. Fig. S1. Distribution of children with chronic cough across Mainland China. NOTE: Red star in the map represents Beijing City. The map was developed in XL Toolbox NG by ourselves, without the conflict of copyright. Fig. S2. Pooled chronic cough prevalence of adults stratified by region. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S3. Pooled chronic cough prevalence of adults stratified by diagnostic criteria. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S4. Pooled chronic cough prevalence of adults stratified by year of publication. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S5. Pooled chronic cough prevalence of adults stratified by age. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S6. Pooled chronic cough prevalence of adults stratified by sampling methods. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S7. Pooled chronic cough prevalence of adults stratified by sample size. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S8. Pooled chronic cough prevalence of adults stratified by prevalence definitions. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S9. Pooled chronic cough prevalence of adults stratified by chronic cough definitions. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S10. Pooled chronic cough prevalence of adults stratified by quality of articles assessed by AHRQ. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S11. Pooled chronic cough prevalence of children stratified by region. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S12. Pooled chronic cough prevalence of children stratified by diagnostic criteria. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S13. Pooled chronic cough prevalence of children stratified by year of publication. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S14. Pooled chronic cough prevalence of children stratified by sample size. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S15. Pooled chronic cough prevalence of children stratified by chronic cough definitions. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S16. Pooled chronic cough prevalence of children stratified by quality of articles assessed by AHRQ. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S17. Pooled chronic cough prevalence of children stratified by prevalence definitions. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S18. Funnel plot for prevalence in studies of adults for chronic cough. Fig. S19. Sensitivity analysis for prevalence in studies of adults for chronic cough. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S20. The prevalence of chronic cough in adults after exclusion of the nationwide study (Li JC 2018). Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S21. The prevalence of chronic cough in adults after exclusion of the low prevalence study (ZHANG JF 1999). Abbreviations: CI, confidence intervals. NOTE: The two author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S22. Funnel plot for prevalence in studies of children for chronic cough. Fig. S23. Sensitivity analysis for prevalence in studies of children for chronic cough. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S24. Pooled prevalence of chronic cough in China (including adults and children). Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, the two author labels of Venners 2001 are from the same literature, and the four author labels of ZHANG JF 2002 are from the same literature

    Joint faulting behaviour of innovative short concrete slabs

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    Pavements are one of the largest assets of a city and their functional condition (ride quality) is priority for their clients. In jointed plain concrete pavements (JPCPs), the presence of joint faulting (JF) reduces the ride quality. Today, short slabs are available as a cost-effective JPCP innovation. The objective of this paper is to analyse the JF behaviour of JPCPs with short slabs. For this, a deterioration model to predict it and trends of JF observed in short slabs of Chile and the United States are considered. The HDM-4 model always yields lower JF per joint in short slabs than in traditional ones. However, real-world short slabs show not only lower JF per joint (that the modelled JF), but also that more joints do not necessarily mean more JF per length of pavement that affect the ride quality. One of the relevant explanatory factors for it is the radical reduction of crack width at joints, which produces a fundamental increase of the load transfer efficiency. To maintain favourable behaviour observed in the field it is recommended to assure joint activation and to provide adequate stiffness of the layers below the short slabs.Pavement Engineerin
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