9,776 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Get PDFThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Current concepts on oxidative/carbonyl stress, inflammation and epigenetics in pathogenesis of chronic obstructive pulmonary disease
Get PDFChronic obstructive pulmonary disease (COPD) is a global health problem. The current therapies for COPD are poorly effective and the mainstays of pharmacotherapy are bronchodilators. A better understanding of the pathobiology of COPD is critical for the development of novel therapies. In the present review, we have discussed the roles of oxidative/aldehyde stress, inflammation/immunity, and chromatin remodeling in the pathogenesis of COPD. An imbalance of oxidants/antioxidants caused by cigarette smoke and other pollutants/biomass fuels plays an important role in the pathogenesis of COPD by regulating redox-sensitive transcription factors (e.g., NF-κB), autophagy and unfolded protein response leading to chronic lung inflammatory response. Cigarette smoke also activates canonical/alternative NF-κB pathways and their upstream kinases leading to sustained inflammatory response in lungs. Recently, epigenetic regulation has been shown to be critical for the development of COPD because the expression/activity of enzymes that regulate these epigenetic modifications have been reported to be abnormal in airways of COPD patients. Hence, the significant advances made in understanding the pathophysiology of COPD as described herein will identify novel therapeutic targets for intervention in COPD
A triazole derivative elicits autophagic clearance of polyglutamine aggregation in neuronal cells [Corrigendum]
No full textHsieh CH, Lee LC, Leong WY, Yang TC, Yao CF, Fang K. Drug Des Devel Ther. 2016;10:2947–2957.On page 2950, Figure 1, part B has been updated.Read the original article 
Quality control method for Yao medicine Hongniuxi
No full textYao medicine Hongniuxi is the root and rhizome of Achyranthes longifolia, which is a commonly used medicinal herb in Yao ethnic areas. Currently, only a few provinces and regions have recorded the method for quality control of Hongniuxi. Microscopic method and thin layer chromatography (TLC) were qualitatively identified on the roots and rhizomes of 14 batches of Yao Medicine Hongniuxi collected in Guangxi Zhuang Autonomous Region in the study. The content of β-ecdysterone was determined by high performance liquid chromatography (HPLC). The Phenomenex column (250 mm × 4.6 mm, 5 μm) was used, the mobile phase was acetonitrile-water (15∶85), the column temperature was 25 ℃, the flow rate was 1.0 mL·min-1, and the detection wavelength was at 243 nm. The results were as follows: (1) The character and microscopic identification methods of Hongniuxi were obvious. Microscopic identification showed the inner layer of thrombus, vascular bundle, abnormal vascular bundle, duct, ray cell, calcium oxalate sand crystal or square crystal, etc. (2) The TLC identification spots of β-ecdysterone and ginsenoside RO were clear, and the separation effect was good. (3) The β-ecdysterone showed a good linear relationship within the range of 5.606-224.224 μg (r＝0.999 9), whose average recovery (n=6) was 97.68%, with the RSD of 1.37%. (4) Among 14 batches of samples, the contents of β-ecdysterone ranged from 0.005% to 0.158%, with an average content of 0.095%. This study establishes a complete method for microscopic identification, thin layer identification, and content determination, providing a scientific basis for rational evaluation of the quality of Hongniuxi and clinical rational drug use
Psychometric Properties of Two Simplified 3-point Balance Scales in Patients with Stroke.
No full textSynthesis of indolo[3,2-b]carbazole-based random copolymers for polymer solar cell applications
No full text[[abstract]]In addition to preparing two indolocarbazole-based random copolymers (named as r-PICTBT1 and r-PICTBT2), this work investigated their feasibility for bulk heterojunction polymer solar cells (PSCs). These copolymers consisted of commercially available 3,9-dibromo-5,11-dioctyl-5,11-dihydroindole[3,2-b]carbazole, 2,5-bis(trimethylstannyl) thiophene and dibromobenzo[c][1,2,5]thiadiazole by varying the feed in ratios via Stille cross-coupling reactions. The photophysical and electrochemical properties of the resulting copolymers could be fine-modulated easily by adjusting the feed ratios of monomers. Both copolymers in the thin film state exhibited two obvious peaks and a vibronic shoulder in the absorption spectra. Electrochemical experiments indicated that the highest occupied molecular orbital energy levels were -4.95, -5.00 eV; meanwhile, the lowest unoccupied molecular orbital energy levels were -3.38, -3.54 eV for r-PICTBT1 and r-PICTBT2, respectively. Bulk heterojunction PSCs composed of anelectron-donor copolymer blended with an electron acceptor [6,6]-phenyl-C61-butyric acid methyl ester (PC61BM) or [6,6]-phenyl-C71-butyric acidmethyl ester (PC71BM) at a weight ratio of 1: 1 or 1: 3 were investigated. Moreover, the r-PICTBT2/PC71BM-based (w/w = 1: 1) PSC performed the best with an open-circuit voltage of 0.54 V, short-circuit current of 6.83 mA/cm(2), fill factor of 0.44, and power conversion efficiency of 1.63%. (C) 2013 Elsevier B. V. All rights reserved.[[note]]SC
Les diminutifs basques avec ch
No full textSe presentan formaciones similares a los diminutivos vascos con "ch" en España y América latina. Se dan ejemplosThe author introduces similar formations to the Basque diminutive "ch" in Spain and Latin America. Examples are provide
COUPLING OF THE C-H STRETCH TO LARGE-AMPLITUDE TORSION AND INVERSION MOTIONS: COMPARISON OF CHCH, CHOH AND CHNH
No full textAuthor Institution: Department of Polymer Science and Department of Chemistry, The University of Akron; Department of Chemistry, The University of Akron, OH 44325In each of the title molecules, torsional and inversion tunneling occurs between six equivalent minima. Coupling of these degrees of freedom to the CH stretch occurs via variation of the C-H stretching force constants as a function of the torsional () and inversion () angles. Maps of the couplings have been computed at the MP2/6-311++G(3df,2p) level. Both the single bond CH stretch force constants and the bilinear couplings between CH bonds are presented as a function of and . Although the torsional barriers differ by more than a factor of 20, the torsion-inversion-vibration coupling patterns are very similar for CHNH and CHCH. On the other hand, the torsion-inversion-vibration coupling in the charged species CHOH is much weaker
Reply to the Ch. Lagrange’s note
Get PDFThe author answers to a Ch. Lagrange’s note who refutes his theory on the diurnal nutation.L’auteur répond à une note de Ch. Lagrange qui réfute sa théorie sur la nutation diurne
NATURE OF TORSION-INVERSION COUPLING IN CHNH, CHOH AND CHCH
No full textAuthor Institution: Department of Chemistry, The University of Akron, OH; 44325-3601Two-dimensional torsion-inversion surfaces for methylamine, protonated methanol and ethyl radical were calculated and fit to a function containing a polynomial in the inversion angle() and trigonometric functions of the torsional angle(). Calculations were done at the B3LYP, MP2, and CCSD(T) levels with the 6-311++G(d,p) and 6-311++G(3df, 2p) basis sets and partial optimization. CHNH, CHOH and CHCH have G symmetry with 6-equivalent minima which are located by the various calculations at inversion angles 6.5 to 11; 42 to 45.5 and 52.5 to 55 degrees respectively on either side of planar. The three molecules have very different barriers to inversion ranging from no barrier for CHCH to 838 cm for CHOH to 1837 cm for CHNH. The dominant torsion-inversion coupling term in all cases has the form
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