996 research outputs found
Hx-amides: DNA Sequence Recognition by the Fluorescent Hx (p-anisylbenzimidazole)•Pyrrole and Hx•Imidazole Pairings
No full textHx-amides are fluorescent hybrids of imidazole (1)- and pyrrole (P)-containing polyamides and Hoechst 33258, and they bind in the minor groove of specific DNA sequences. Synthesis and DNA binding studies of HxII (5) complete our studies on the first set of Hx-amides: Hx-I/P-I/P. HxPP (2), HxIP (3) and HxPI (4) were reported earlier. Results from DNase I footprinting, biosensor-SPR, CD and Delta T-M studies showed that Hx-amides interacted with DNA via the anti-parallel and stacked, side-by-side motif. Hx was found to mimic the DNA recognition properties of two consecutive pyrrole units (PP) in polyamides. Accordingly, the stacked Hx/PP pairing binds preferentially to two consecutive AT base pairs, A/T-A/T; Hx/IP prefers C-A/T; Hx/PI prefers A/T-C; and Hx/II prefers C-C. The results also showed that Hx-amides bound their cognate sequence at a higher affinity than their formamido-triamide counterparts. (C) 2013 Elsevier Ltd. All rights reserved
Fe powder catalyzed highly efficient synthesis of alkenyl halides via direct coupling of alcohols and alkynes with aqueous HX as exogenous halide sources
No full textA simple and efficient catalytic method for the synthesis of alkenyl halides via direct coupling of alcohols and alkynes using aqueous HX (X=Cl, Br) as halide sources has been developed under mild conditions in the presence of Fe powder (1 mol %). In comparison with the high loading of FeX3 in previously reported protocols, the present approach provides a remarkable attractive methodology to a diverse range of alkenyl halides due to the advantages of simple operation and low-level metal contamination. (C) 2015 Elsevier Ltd. All rights reserved
Improving the performance of outbreak detection algorithms by classifying the levels of disease incidence
No full textWe evaluated a novel strategy to improve the performance of outbreak detection algorithms, namely setting the alerting threshold separately in each region according to the disease incidence in that region. By using data on hand, foot and mouth disease in Shandong province, China, we evaluated the impact of disease incidence on the performance of outbreak detection algorithms (EARS-C1, C2 and C3). Compared to applying the same algorithm and threshold to the whole region, setting the optimal threshold in each region according to the level of disease incidence (i.e., high, middle, and low) enhanced sensitivity (C1: from 94.4% to 99.1%, C2: from 93.5% to 95.4%, C3: from 91.7% to 95.4%) and reduced the number of alert signals (the percentage of reduction is C1?4.3%, C2?11.9%, C3?10.3%). Our findings illustrate a general method for improving the accuracy of detection algorithms that is potentially applicable broadly to other diseases and regions
Diasporas and democratization in the post-communist world
No full textIf diaspora communities are socialized with democratic values in Western societies, they could be expected to be sympathetic to the democratization of their home countries. However, there is a high degree of variation in their behavior. Contrary to the predominant understanding in the literature that diasporas act in exclusively nationalist ways, this article argues that they do engage with the democratization of their home countries. Various challenges to the sovereignty of their homelands explain whether diasporas involve with procedural or liberal aspects of democratization. Drawing evidence from the activities of the Ukrainian, Serbian, Albanian and Armenian diasporas after the end of communism, I argue that unless diasporas are linked to home countries that enjoy both international legal and domestic sovereignty, they will involve only with procedural aspects of democratization. Diasporas filter international pressure to democratize post-communist societies by utilizing democratic procedures to advance unresolved nationalist goals
Absolute parameters of young stars: HX Velorum
No full textWe present combined photometric and spectroscopic analyses of the southern multiple star HX Vel. High-resolution spectra of this system were taken at the University of Canterbury Mt. John Observatory in the years 2009-2015. Absolute parameters for HX Vel tend to confirm its young and near-main-sequence nature. We specify the main adopted parameters of the (non-eclipsing) close binary as follows: M-1 = 8.5 +/- 1.7, M-2 = 5.4 +/- 1.2, R-1 = 5.0 +/- 0.3, R-2 = 3.1 +/- 0.2, (circle dot); T-1 25 000 +/- 1300, T-2 20 000 +/- 2500 (K); systemic M-V -4.2 +/- 0.2. These parameters are sensitive to the low inclination, where we find a small disparity between the photometrically optimal 28 +/- 2 degrees and an adopted value of 26 degrees that gives better consistency with main-sequence stellar modelling. The relationship of HX Vel to the galactic cluster IC 2395 and OB association Vela OB1C is also considered, in the context of photometric parallaxes found for HX Vel and other cluster members.School of Chemical and Physical Sciences of the Victoria University of Wellington; Royal Astronomical Society of New Zealand; Variable Stars South sectionGenerous allocations of time on the 1m McLennnan Telescope and HERCULES spectrograph at the University of Canterbury Mt John Observatory in support of the Southern Binaries Programme have been made available through its TAC and supported by its Director, Dr. K. Pollard and previous Director, Prof. J. B. Hearnshaw. Useful help at the telescope were provided by the UCMJOmanagement (N. Frost and previously A. Gilmore and P. Kilmartin). Considerable assistance with the use and development of the HRSP software (leading up to the latest version 5) was given by its author Dr. J. Skuljan. Encouragement and support for this programme have been shown by the the School of Chemical and Physical Sciences of the Victoria University of Wellington, as well as the Royal Astronomical Society of New Zealand and its Variable Stars South section (http://www.variablestarssouth.org)
The moderating effect of corporate environmental responsibility on relational capital and green innovation: evidence from a knowledge-driven context
No full textPurposeGiven that environmental issues have become increasingly critical in business operations, from the lens of guanxi, this study explores the impact of relational capital on green innovation in a knowledge-driven context of new energy enterprises. Additionally, the moderating effect of corporate environmental responsibility (CER) on the above relationship is analyzed.Design/methodology/approachThis study takes 162 Chinese new energy enterprises from 2010 to 2020 as the research sample. For empirical analysis, factor analysis is adopted to comprehensively measure relational capital, while green innovation is embodied in two dimensions, namely radical green innovation (RGI) and incremental green innovation (IGI).FindingsRelational capital significantly promotes RGI and IGI. Moreover, it is found that implementing CER strengthens the positive relationship between relational capital and RGI but weakens the positive relationship between relational capital and IGI.Originality/valueIt is evident that existing literature on green innovation mainly focused on a single perspective rather than from different dimensions. In addition, few scholars have drawn from stakeholder theory to elucidate the interaction of relational capital with corporate responsibility practices. In this regard, this study examines the link between relational capital and green innovation while examining the moderating effect of CER, which provides valuable insights for future research on relational governance and innovation management. Furthermore, this study innovatively centers on new energy enterprises in China, which are pioneers and facilitators of green development, as the research subject. Considering relevant studies are still nascent in this domain, our empirical results are of extensive practical guidance for managers and practitioners to promote environmental sustainability
Role of dysbindin in dopamine receptor trafficking and cortical GABA function.
No full textDysbindin has been implicated in the pathogenesis of schizophrenia, but little is known about how dysbindin affects neuronal function in the circuitry underlying psychosis and related behaviors. Using a dysbindin knockout line (dys(-/-)) derived from the natural dysbindin mutant Sandy mice, we have explored the role of dysbindin in dopamine signaling and neuronal function in the prefrontal cortex (PFC). Combined cell imaging and biochemical experiments revealed a robust increase in the dopamine receptor D2, but not D1, on cell surface of neurons from dys(-/-) cortex. This was due to an enhanced recycling and insertion, rather than reduced endocytosis, of D2. Disruption of dysbindin gene resulted in a marked decrease in the excitability of fast-spiking (FS) GABAergic interneurons in both PFC and striatum. Dys(-/-) mice also exhibited a decreased inhibitory input to pyramidal neurons in layer V of PFC. The increased D2 signaling in dys(-/-) FS interneurons was associated with a more pronounced increase in neuronal firing in response to D2 agonist, compared to that in wild-type interneurons. Taken together, these results suggest that dysbindin regulates PFC function by facilitating D2-mediated modulation of GABAergic function
SENGKETA PEMBEBANAN TARIF BEA MASUK ATAS KESALAHAN PENETAPAN HS CODE BARANG IMPOR: (Studi Kasus Impor HMI PT. HX)
No full textHarmonized System Code (HS Code) adalah komponen penting yang digunakan dalam mengidentifikasi barang impor maupun ekspor. Kenyataannya di PT. HX. kesalahan dalam penetapan HS Code mengakibatkan perbedaan tarif bea masuk dan pajak yang berbeda-beda setiap tahunnya untuk barang HMI, sehingga menimbulkan sengketa. Metode penelitian bertujuan untuk mendeskripsikan penetapan HS Code HMI di PT. HX serta meninjau keputusan Direktur Jenderal Bea dan Cukai atas pengajuan keberatan PT. HX. Penelitian ini menggunakan metode penelitian hukum normatif yang dilakukan dengan pendekatan Undang-Undang serta dilakukan dengan cara studi wawancara dan beberapa dokumen pendukung lainnya berdasarkan data yang tersedia. Adapun teknik analisis data dalam penelitian ini dilakukan secara kualitatif. Hasil penelitian ini menunjukan adanya perbedaan tarif bea masuk yang disebabkan kesalahan dalam menetapkan HS Code barang sehingga menambah tarif bea masuk sebesar 10%. Hal ini mengakibatkan PT. HX mengajukan keberatan ke Direktorat Jenderal Bea dan Cukai. Untuk menghindari terjadinya kesalahan dalam penetapan HS Code, importir perlu berkomunikasi dengan pihak eksportir dan Pejabat Bea Cukai mengenai pengklasifikasian kode barang yang benar
Exploring bio-remediation strategies by a novel bacteria Micrococcus sp. strain HX in Cr(VI)-contaminated groundwater from long-term industrial polluted
No full textHexavalent chromium (Cr(VI)) has emerged as a contaminant of heavy metal, owing to its wide use in industry. This study focuses on elucidating the interaction between microbial communities and environmental parameters in Cr(VI)-contaminated groundwater near a factory in Henan Province, and evaluating the bio-remediation potential of microorganisms toward Cr(VI) reduction. The highest concentration of Cr(VI) in the groundwater is 208.08 mg/L. The dominant microbes were Proteobacteria and Bacteroidota, closely positively related to Cr(VI) and SO42-. Many of these genus have been proven to be chromium tolerant or have the ability to reduce Cr(VI). Two strains, Micrococcus sp. HX and Bacillus sp. HX-2, were isolated from contaminated groundwater, and Micrococcus sp. HX was used for the first time to reduce Cr(VI) in groundwater. The reduced ability of HX reached 90.18 % at a Cr(VI) concentration of 100 mg/L, while HX-2 achieved a reduction capacity of 63.8 %. Micrococcus sp. HX shows the best reduction efficiency in alkaline environments (ph=8), which is close to the tannery industry wastewater. The reduction efficiency by Micrococcus sp. HX reached 67.26 % in groundwater samples (Cr(VI)= 26.08 mg/L). Transcriptome analyses revealed oxidoreductase activity, ATP binding and the NAD(P) binding region protein-related gene expression were up-regulated. Binding reduction experiments indicated that most of the Cr(III) was detected extracellular, which suggests that the reduction of Cr(VI) by HX was mainly extracellular enzyme-catalyzed
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