14 research outputs found
O papel do ensino de geografia no fortalecimento da educação do campo e na (re)construção do território camponês local
No full textTese (doutorado)—Universidade de Brasília, Instituto de Ciências Humanas, Departamento de Geografia, Programa de Pós-Graduação em Geografia, 2018.Esta tese se propõe a analisar o papel do Ensino de Geografia desenvolvido na Escola Família Agrícola de Natalândia – EFAN, Minas Gerais, no fortalecimento da Educação do Campo e na (re)construção do território camponês local. Nossa suposição é a de que o Ensino de Geografia contribui para a formação dos sujeitos e auxilia no fortalecimento da sua identidade e do seu território. Assim, esta investigação, de base qualitativa, foi realizada por meio de: análise documental; entrevista semiestruturada com o monitor da disciplina de Geografia, o coordenador pedagógico, a supervisora do Ensino Médio e duas lideranças da comunidade; roda de conversa com os alunos do 3º ano do Ensino Médio, e construção do diário de campo. Além disso, participamos, em outros momentos, de eventos e espaços de formação durante dois anos de vivências com os sujeitos locais. Na investigação, abordamos os seguintes temas/autores: questão agrária brasileira, em Alentejano (2014) e Oliveira (2001, 2012); o campesinato e o conceito de Camponês, em Fernandes (2009, 2013); Território, em Haesbaert (2016); o movimento da Educação do Campo, a partir de Caldart e Molina (2011), Caldart et al (2012), Caldart (2015), Freitas (2010) e Molina (2012); e a construção de um arcabouço teórico sobre o Ensino de Geografia em Escolas do Campo, a partir de Callai (2001, 2005), Cavalcanti (2010, 2012), Castellar (2015) e Leite (2002, 2012). Os resultados evidenciaram como se deu a apropriação e construção do território camponês local e a materialização da EFAN enquanto um território educativo. Além disso, ficou evidente, também, a importância do Ensino de Geografia desenvolvido na escola para a formação dos sujeitos, o resgate e valorização da sua identidade e o fortalecimento do território camponês local, embora pudesse ser potencializada caso não houvesse negligência em relação a alguns dos conceitos e conteúdos fundamentais da ciência geográfica. Por fim, em conjunto com a equipe pedagógica, foram definidos os conteúdos fundamentais para cada ano de formação do Ensino Médio, considerados necessários para uma aproximação da Geografia com a Educação do Campo, especificamente para o contexto da EFAN. Concluímos que o Ensino de Geografia, quando entendido enquanto uma construção realizada em conjunto com os sujeitos, através de processos que prezem pela autonomia, empoderamento e valorização das especificidades e dos sujeitos locais, tem papel fundamental no fortalecimento e na construção da Educação do Campo, e contribui, principalmente, para a materialização de processos que valorizem os tempos e espaços do campo e dos sujeitos camponeses. Na Geografia, em especial, essa significação potencializa o trabalho com seus temas e conteúdos; por um lado, auxilia no processo de fortalecimento da cultura e da identidade do camponês, e, por outro, na reconstrução e/ou ressignificação dos conhecimentos geográficos a partir da realidade dos sujeitos, algo tão caro à ciência geográfica.This thesis proposes to analyze the role of Geography Teaching as it is developed at the Escola Família Agrícola de Natalândia - EFAN, Minas Gerais, in the strengthening of the Educação do Campo and in the (re)construction of the local peasant territory. We believe that Geography Teaching contributes to the subjects‟ formation and enhances the strengthening of their identity and territory. This qualitative research was carried out through documentary analysis; semi-structured interviews with the Geography Monitor, the pedagogical coordinator, the High School supervisor and two community leaders; conversation with students of EFAN‟s High School and field diary. In addition, the author has participated in events and formation spaces during the two years of experiences with local subjects. The following themes and authors were approached: the Brazilian agrarian question, in Alentejano (2014) and Oliveira (2001; 2012); the peasantry and the Peasant concept, in Fernandes (2009, 2013); Territory in Haesbaert (2016); the Educação do Campo movement, in Caldart and Molina (2011), Caldart et al (2012), Caldart (2015), Freitas (2010) and Molina (2012); the construction of a theoretical framework on the Teaching of Geography in Escolas do Campo, from Callai (2001, 2005), Cavalcanti (2010, 2012), Castellar (2015) and Leite (2002, 2012). The results showed how the appropriation and construction of the local peasant territory and the materialization of the EFAN as an educational territory took place. In addition, the importance of Geography Teaching for the subjects‟ formation, the rescue and valorization of their identity and the strengthening of the local peasant territory was made evident, although it could be enhanced if there were no negligence of some fundamental concepts and contents of geographic science. Finally, we propose basic contents for each year of High School education specifically for the EFAN context, which were developed with the school‟s pedagogical team. The conclusion is that Geography Teaching, when understood as a construction carried out in conjunction with the subjects, through processes that cherish autonomy, empowerment and valorization of local specificities and subjects, plays a fundamental role in the strengthening and construction of the Educação do Campo, and contributes to the materialization of processes that value the peasant subjects. For the geographic science, on one hand, this understanding helps in the process of strengthening the culture and the identity of the peasant, and, on the other hand, it allows the reconstruction and re-signification of the geographical knowledge from the reality of the subjects.Instituto de Ciências Humanas (ICH)Departamento de Geografia (ICH GEA)Programa de Pós-Graduação em Geografi
ANALYSIS OF PRODUCT PLACEMENT AS A GLOBAL MARKETING STRATEGY OF SUBWAY SANDWICH IN DRAMA MY ROOMMATE IS GUMIHO
No full textSubway is a fast food restaurant from America that uses a franchise concept. This study aims to determine the Product Placement used by Subway Sandwich as a global marketing strategy in the Korean drama My Roommate is Gumiho. In this study, the author uses a qualitative method in social phenomena by collecting data from news, journals, articles, dramas, and books to find evidence that follows product placement and global marketing strategies. However, this study focuses on analyzing Korean dramas and news articles. The findings of this study will produce a division of product placement dimensions contained in the drama My Roommate is Gumiho. The result of this study is product placement carried out by Subway in the parts contained in the drama My Roommate, which means international audiences can increasingly recognize Subway products
Implementación de la metodología del plan de negocios en mipyme “Zona Green”
No full textThrough the present analysis in the Micro-enterprise called "Green Zone" the methods for the implementation of the business plan were analyzed as: the mission and the vision giving a greater purpose of the business and its administration. Likewise, other analyzes are applied to detect which are the current clients to improve the organization and to measure the result in the business. A financial analysis was also established to discover how this business works and offers an improvement in a way that facilitates the business process. Applying a SWOT analysis were discovered the several of which one of the most prominent were the strengths of the companies stands out the location of the business. All previously confirmed information has been cited in order to grant credit to the author and support the credibility of the document, whose purpose is to improve the business.A través del presente análisis en la Micro empresa llamada “Zona Green” se analizaron diferentes aspectos para la implementación del plan de negocios tales como: misión y visión, dando a conocer el principal propósito del negocio y su administración. Asimismo, se aplicaron otros análisis para detectar cuales son los actuales consumidores para mejorar la organización y medir los resultado en el negocio. También se estableció un análisis financiero para descubrir la manera en que trabaja este negocio y ofrecer una mejora de manera que facilite el proceso del negocio. Aplicando un análisis FODA se descubrieron varios aspectos de los cuales uno de los más destacados fueron las Fortalezas de las cuales sobresale la ubicación del negocio. Se ha citado toda la información previamente confirmada con el fin de otorgar crédito al autor y apoyando la credibilidad del documento, cuyo fin es la mejora del negocio
Alleviation of cold damage to photosystem II and metabolisms by melatonin in Bermudagrass
No full textAs a typical warm-season grass, Bermudagrass [Cynodon dactylon (L).Pers.] is widely applied in turf systems and animal husbandry. However, cold temperature is a key factor limiting resource utilization for Bermudagrass. Therefore, it is relevant to study the mechanisms by which Burmudagrass responds to cold. Melatonin is a crucial animal and plant hormone that is responsible for plant abiotic stress responses. The objective of this study was to investigate the role of melatonin in cold stress response of Bermudagrass. Wild Bermudagrass pre-treated with 100 μM melatonin was subjected to different cold stress treatments (-5 °C for 8 h with or without cold acclimation). The results showed lower malondialdehyde (MDA) and electrolyte leakage (EL) values, higher levels of chlorophyll, and greater superoxide dismutase and peroxidase activities after melatonin treatment than those in non-melatonin treatment under cold stress. Analysis of chlorophyll a revealed that the chlorophyll fluorescence transient (OJIP) curves were higher after treatment with melatonin than that of non-melatonin treated plants under cold stress. The values of photosynthetic fluorescence parameters increased after treatment with melatonin under cold stress. The analysis of metabolism showed alterations in 46 metabolites in cold-stressed plants after melatonin treatment. Among the measured metabolites, five sugars (arabinose, mannose, glucopyranose, maltose, turanose) and one organic acid (propanoic acid) were significantly increased. However, valine and threonic acid contents were reduced in melatonin-treated plants. In summary, melatonin maintained cell membrane stability, increased antioxidant enzymes activities, improved the process of photosystem II, and induced alterations in Bermudagrass metabolism under cold stress
Burst of reactive oxygen species (ROS) in pedicel mediated fruit abscission after carbohydrate supply was cut off in longan (Dimocarpus longan)
No full textCutting off carbohydrate supply to longan (Dimocarpus longan Lour.) fruit by girdling and defoliation or by detachment induced 100% abscission within a few days. We used these treatments to study the involvement of reactive oxygen species (ROS) in fruit abscission. Girdling plus defoliation decreased sugar concentrations in the fruit and pedicel and depleted starch grains in the chloroplasts in the cells of abscission zone. Prior to the occurrence of intensive fruit abscission, there was a burst in ROS in the pedicel, which peaked at 1 day after treatment (DAT), when H2O2 in the abscission zone was found to be chiefly located along the plasma membrane (PM). H2O2 was found exclusively in the cell walls 2 DAT, almost disappeared 3 DAT, and reappeared in the mitochondria and cell walls 4 DAT. Signs of cell death such as cytoplasm breakdown were apparent from 3 DAT. The burst of ROS coincided with a sharp increase in the activity of PM-bound NADPH oxidase in the pedicel. At the same time, activities of antioxidant enzymes including superoxide dismutase (SOD), catalase and peroxidase (POD) were all increased by the treatment and maintained higher than those in the control. Accompanying the reduction in H2O2 abundance, there was a sharp decrease in PM-bound NADPH oxidase activity after 1 DAT in the treated fruit. H2O2 scavenger dimethylthiourea (DMTU, 1 g L-1) significantly inhibited fruit abscission in detached fruit clusters and suppressed the increase in cellulase activity in the abscission zone. These results suggest that fruit abscission induced by carbohydrate stress is mediated by ROS. Roles of ROS in regulating fruit abscission were discussed in relation to its subcellular distribution
Effect of heavy metals pollution on soil microbial diversity and bermudagrass genetic variation
No full textHeavy metal pollution is a serious global environmental problem as it adversely affects plant growth and genetic variation. It also alters the composition and activity of soil microbial communities. The objectives of this study were to determine the soil microbial diversity, bermudagrass genetic variation in Cd contaminated or uncontaminated soils from Hunan province of China, and to evaluate Cd tolerance of bermudagrass at different soils. The Biolog method, hydroponic experiments and simple sequence repeat markers were used to assess the functional diversity of microorganisms, Cd tolerance and the genetic diversity of bermudagrass, respectively. Four of the sampling sites were heavily contaminated with heavy metal. The total bioactivity, richness, and microbial diversity decreased with increasing concentration of heavy metal. The hydroponic experiment revealed that bermudagrass populations collected from polluted sites have evolved, encompassing the feature of a higher resistance to Cd toxicity. Higher genetic diversity was observed to be more in contaminated populations than in uncontaminated populations. Heavy metal pollution can result in adverse effects on plant growth, soil microbial diversity and activity, and apparently has a stronger impact on the genetic structure. The results of this study provide new insights and a background to produce a genetic description of populations in a species that is suitable for use in phytoremediation practices
A novel pentapeptide targeting integrin β3-subunit inhibits platelet aggregation and its application in rat for thrombosis prevention
No full textBackground: Antiplatelet therapy plays a pivotal role in the prevention and treatment of thrombotic diseases. We reported the screening of P1C as a novel integrin-binding peptide from the C-terminal of connective tissue growth factor. Primary study indicated that P1C has potential against platelet aggregation. Objectives: In this study, we aimed to find the shortest active unit from the P1C fragments and explore its in vivo and in vitro activities. Methods: A series of truncated P1C fragments was prepared and screened for antiplatelet activity. The most active fragment was evaluated using coagulation assays. Flow cytometry and confocal microscopy were used to determine the interaction between the peptide and the integrin. The in vivo potential was further explored using two types of rat models. Results: From a series of truncated P1C forms, a so-called P1Cm peptide of 5-amino acids, namely, IRTPK, was screened out as the shortest active unit with superior activity. Coagulation experiments and an in vivo toxicity assay demonstrated that P1Cm is safe in vivo and inhibits ADP- and TH-induced human platelet aggregation in vitro in a concentration-dependent manner. Furthermore, it has limited effect on the coagulation parameters. Flow cytometry and confocal microscopy experiments consistently indicated that the peptide specifically binds the β3-subunit of integrin on platelets. Further experiments using rat models of artery-vein shunt and carotid arterial thrombosis illustrated that P1Cm can effectively prevent thrombosis formation. Conclusion: All the results suggested that P1Cm may be a new, promising antithrombotic alternative to currently available antiplatelet treatments
A hybrid peptide PTS that facilitates transmembrane delivery and its application for the rapid in vivo imaging via near-infrared fluorescence imaging
No full textBackground and purpose: Intravital imaging provides invaluable readouts for clinical diagnoses and therapies and shows great potential in the design of individualized drug dosage regimes. Ts is a mammalian free cell membrane-penetrating peptide. This study aimed to introduce a novel approach to the design of a cancer-selective peptide on the basis of a membrane-penetrating peptide and to explore its potential as a carrier of medical substances. Experimental approach: Ts was linked with a αvβ3-binding peptide P1c to create a hybrid referred to as PTS. The hybrid was labeled with an FITC or Cy5.5 as an imaging indicator to evaluate its in vitro and in vivo bioactivity. Key results: Hemolysis tests proved that in comparison with Ts, PTS caused similar or even less leakage of human erythrocytes at concentrations of up to 1 mmol/L. Flow cytometry assay and confocal microscopy demonstrated the following. 1) P1c alone could target and mostly halt at the cancer cell membrane. 2) Ts alone could not bind to the membrane sufficiently. 3) P1c greatly enhanced the binding affinity of PTS with MDA-MB-231 breast cancer cells that upregulated αvβ3. 4) Ts conferred PTS with the ability to traverse a cell membrane and thus facilitate the transmembrane delivery of imaging probes. In vivo near-infrared fluorescence (NIRF) imaging demonstrated that the imaging probes were rapidly concentrated in a MDA-MB-231 tumor tissue within 1 h after intravenous injection. Conclusions and implications: PTS exhibited the capability of targeting specific tumors and greatly facilitating the transmembrane delivery of imaging probes
Cultural influences on social feedback processing of character traits
No full textCultural differences are generally explained by how people see themselves in relation to social interaction partners. While Western culture emphasizes independence, East Asian culture emphasizes interdependence. Despite this focus on social interactions, it remains elusive how people from different cultures process feedback on their own (and on others') character traits. Here, participants of either German or Chinese origin engaged in a face-to-face interaction. Consequently, they updated their self- and other-ratings of 80 character traits (e.g., polite, pedantic) after receiving feedback from their interaction partners. To exclude potential confounds, we obtained data from German and Chinese participants in Berlin (functional magnetic resonance imaging) and in Beijing (behavior). We tested cultural influences on social conformity, positivity biases, and self-related neural activity. First, Chinese conformed more to social feedback than Germans (i.e., Chinese updated their trait ratings more). Second, regardless of culture, participants processed self- and other-related feedback in a positively biased way (i.e., they updated more toward desirable than toward undesirable feedback). Third, changes in self-related medial prefrontal cortex activity were greater in Germans than in Chinese during feedback processing. By investigating conformity, positivity biases, and self-related activity in relation to feedback obtained in a real-life interaction, we provide an essential step towards a unifying framework for understanding the diversity of human culture
The global reciprocal reprogramming between mycobacteriophage SWU1 and mycobacterium reveals the molecular strategy of subversion and promotion of phage infection
No full textBacteriophages are the viruses of bacteria, which have contributed extensively to our understanding of life and modern biology. The phage-mediated bacterial growth inhibition represents immense untapped source for novel antimicrobials. Insights into the interaction between mycobacteriophage and Mycobacterium host will inform better utilizing of mycobacteriophage. In this study, RNA sequencing technology (RNA-seq) was used to explore the global response of Mycobacterium smegmatis mc2 155 at an early phase of infection with mycobacteriophage SWU1, key host metabolic processes of M. smegmatis mc2 155 shut off by SWU1, and the responsible phage proteins. The results of RNA-seq were confirmed by Real-time PCR and functional assay. 1174 genes of M. smegmatis mc2 155 (16.9% of the entire encoding capacity) were differentially regulated by phage infection. These genes belong to six functional categories: (i) signal transduction, (ii) cell energetics, (iii) cell wall biosynthesis, (iv) DNA, RNA, and protein biosynthesis, (v) iron uptake, (vi) central metabolism. The transcription patterns of phage SWU1 were also characterized. This study provided the first global glimpse of the reciprocal reprogramming between the mycobacteriophage and Mycobacterium host
