215 research outputs found
A new contrast enhancement protocol for subtraction coronary computed tomography requiring a short breath-holding time
No full text金沢大学博士（保健学）博士論文本文Full 以下に掲載：Academic Radiology 24 pp.38-44 2017. Elsevier. 共著者：Takayoshi Yamaguchi, Katsuhiro Ichikawa, Daichi Takahashi, Teppei Sugaya, Jungo Furuya, Keiichi Igarashidoctoral thesi
Making it Just in Time: Author-Creator Matsumoto Taiyō
No full textTranslated by Jon Holt and Teppei Fukuda
The first time I can remember encountering Matsumoto Taiyō’s work was probably when he released his short story collection, Blue Spring (Aoi haru - Matsumoto Taiyō tanpenshū [stories published from 1990 to 1993; Shōgakukan, 1993]). All of the stories concern a bunch of young dudes -- full of desires, frustrations, and violent tendencies -- and no chance they can ever get past those things. I thought to myself at that time, “Ah, I bet this stuff means a lot to readers in their teens, but they don’t really do anything for me.” After all, I was a man in my forties, so this stuff wasn’t on my radar as I was busy becoming a grown-up. Keep in mind that Taiyō himself was just in his early twenties. So, it really wasn’t that unnatural for him to write about life like that
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No full textEmerging evidence points to an unexpected diversification of core promoter recognition complexes that serve as important regulators of cell-type specific gene transcription. Here, we report that the orphan TBP-associated factor TAF9B is selectively up-regulated upon in vitro motor neuron differentiation, and is required for the transcriptional induction of specific neuronal genes, while dispensable for global gene expression in murine ES cells. TAF9B binds to both promoters and distal enhancers of neuronal genes, partially co-localizing at binding sites of OLIG2, a key activator of motor neuron differentiation. Surprisingly, in this neuronal context TAF9B becomes preferentially associated with PCAF rather than the canonical TFIID complex. Analysis of dissected spinal column from Taf9b KO mice confirmed that TAF9B also regulates neuronal gene transcription in vivo. Our findings suggest that alternative core promoter complexes may provide a key mechanism to lock in and maintain specific transcriptional programs in terminally differentiated cell types.DOI: http://dx.doi.org/10.7554/eLife.02559.001
On the Shafarevich conjecture (Algebraic Number Theory and Related Topics 2018)
No full textAlgebraic Number Theory and Related Topics 2018. November 26-30, 2018. edited by Takao Yamazaki and Shuji Yamamoto. The papers presented in this volume of RIMS Kôkyûroku Bessatsu are in final form and refereed.The Shafarevich conjecture, known as a geometric analogue of the Hermite-Minkowski theorem, states the finiteness of certain varieties over a fixed number field admitting good reduction away from a fixed finite set of finite places. In the abelian varieties of a fixed dimension case, this conjecture was proved by Faltings-Zarhin and applied to the Mordell conjecture. Moreover, the author proved a certain generalization of this conjecture in the K3 surfaces case. In the first part of this survey, we will sketch the proof of Faltings-Zarhin and its relation to the Mordell conjecture, and the second part we will present the result of the author
Dissecting the roles of histone deacetylase 1 and 2 in the hematopoietic system
No full textReversible histone acetylation on lysine residues has been intensively studied as an epigenetic mark for gene activation in a variety of eukaryotes. Histone deacetylases (HDACs) act to repress gene expression by removing acetyl groups from histones and possibly other proteins. Based on their expression pattern, distribution and biochemical activity, HDAC1 andhave been considered to be the major HDACs to regulate gene expression in a wide range of tissues in mammals. Experiments using transformed cells and HDAC inhibitors suggest that HDACs are important for cell cycle progression, apoptosis regulation and induction of differentiation; however their in vivo roles in a higher organism as well as their molecular mechanism of actions remain largely unexplored. I have generated mice conditionally targeted at the HDAC1 or HDAC2 locus and crossed these to different deleter mice in order to dissect the function of these enzymes in vivo; I focused on B cell specific inactivation in the first part of the thesis and on hematopoieticlineage specific inactivation in the second part. Mice completely lacking HDAC1 die during embryogenesis while animals lacking HDAC2 show partial perinatal mortality. In contrast, ablation of HDAC1 orin the B cell lineage led to no obvious developmental defect. Simultaneous deletion of both HDAC1 andin the B cell lineage causes a very severe blockade in the early B cell development accompanied by severe defects in cell cycle progression, apoptosis regulation and recombination of immunoglobulin heavy chain (IgH) variable (VH) segments. Using three-dimensional DNA fluorescence in situ hybridization, we show that pre-BI cells lacking HDAC1 andare severely impaired in the contraction of the IgH locus, a mechanism whereby distantly located variable genes are brought in close proximity to the rest of the locus. Analysis of histone modifications revealed that these pre-BI cells lshow global histone hyperacetylation as well as increased histone lysinemethylation. Taken together, we conclude that HDAC1 andare crucial factors for B cell development, survival and IgH recombination by modulating histone modifications which may affect gene expression and the higher order of chromatin structure. In the second part I have analyzed the roles of HDAC1 in the hematopoietic lineage. Loss of HDAC1 in the hematopoietic lineage has only minor impacts on the myeloid lineage development, but impaired IgG secretion and reduced germinal center formation were observed upon induction of a T cell dependent immune response. Using transcriptome analysis, we found that these germinal center B cells lacking HDAC1 exhibit higher expression of genes involved in cell cycle regulation as well as apoptosis. These results suggest that HDAC1 has important roles to regulate the adaptive immune response by controlling the expansion of B cells upon antigen stimulation
Circulating salmon 28- and 22-kDa insulin-like growth factor binding proteins (IGFBPs) are co-orthologs of IGFBP-1.
No full textCirculating insulin-like growth factor binding proteins (IGFBPs) play pivotal roles in stabilizing IGFs and regulating their availability to target tissues. In the teleost circulation, three major IGFBPs are typically detected by ligand blotting with molecular masses around 20-25, 28-32 and 40-45kDa. However, their identity is poorly established and often confused. We previously identified salmon 22- and 41-kDa forms as IGFBP-1 and -2b, respectively. In the present study, we cloned the cDNA of 28-kDa IGFBP from Chinook salmon (Oncorhynchus tshawytscha) as well as rainbow trout (Oncorhynchus mykiss) based on the partial N-terminal amino acid sequence of purified protein and identified it as an ortholog of IGFBP-1. Structural and phylogenetic analyses revealed that the 28-kDa IGFBP is more closely related to human IGFBP-1 and zebrafish IGFBP-1a than the previously identified salmon IGFBP-1 (i.e. 22-kDa IGFBP). We thus named salmon 28- and 22-kDa forms as IGFBP-1a and -1b, respectively. Salmon IGFBP-1a contains a potential PEST region involved in rapid protein turnover and phosphorylation sites typically found in mammalian IGFBP-1, although the PEST and phosphorylation scores are not as high as those of human IGFBP-1. There was a striking difference in tissue distribution patterns between subtypes; Salmon igfbp-1a was expressed in a variety of tissues while igfbp-1b was almost exclusively expressed in the liver, suggesting that IGFBP-1a has more local actions. Direct seawater exposure (osmotic stress) of Chinook salmon parr caused increases in both IGFBP-1s in plasma, while IGFBP-1b appeared to be more sensitive. The presence of two co-orthologs of IGFBP-1 in the circulation in salmon, and most likely in other teleosts, provides a good opportunity to investigate subfunction partitioning of duplicated IGFBP-1 during postnatal growth
Time to Re-Evaluate Taniguchi Jiro\u27s Pace in Manga
No full textNatsume Fusanosuke is Emeritus Professor of the Graduate Program of Cultural Studies in Corporeal and Visual Representation, Gakushūin University. Despite his recent retirement from Gakushūin in March, he is still very active in manga criticism and scholarship. Originally a manga artist himself in the 1980s, by the 1990s he began doing more writing about manga, although he often still employs his cartooning skills to assist in his analysis and explanation of his subjects, much like his American contemporary Scott McCloud. It is not a stretch to compare the latter’s Understanding Comics to Natsume\u27s work in the classic How to Read Manga (Manga no yomikata, 1995; co-authored with Takekuma Kentarō and others) and his subsequent Why Is Manga So Interesting? Its Expression and Grammar (Manga wa naze omoshiroi no ka: sono hyōgen to bunpō), which aired originally as a NHK [Japanese public broadcasting] ten-week lecture mini-series. Like McCloud, Natsume pioneered techniques to see and analyze comics that are still in use today by scholars. Although Natsume’s publications are too numerous to list here, he is author and co-author of approximately twenty books on manga and manga scholarship, including monographs like Where Is Tezuka Osamu? (Tezuka Osamu wa doko ni iru, 1992), the first full-length study on the manga giant. After the 1990s, Natsume went on to revise his early approaches to manga study, analysis, and scholarship, as seen in his New Challenges for the Field of Manga (Mangagaku e no chosen, 2004). He also co-edited with Takeuchi Osamu a new reader for Manga Studies, Mangagaku nyūmon (2009). In addition to these achievements, in his career he has been a television host for NHK’s public television show on comics (Broadcast Satellite Manga Night Talks [BS Manga yawa]), and author of other books on Japanese culture, including Grandson of Sōseki (Sōseki no mago, 2003), which tells the story of his family and his connection to Japan’s great modern novelist Natsume Sōseki. In 1999, he was the recipient of the prestigious Tezuka Osamu Culture Award.
In the following essay on Taniguchi Jirō, whom Natsume considers one of Japan’s most important manga creators, Natsume re-envisions the artist in the context of recent manga history. Taniguchi passed away in 2017, but his work suggests a path towards a new kind of “adult reading” of manga, which is a theme of Natsume’s recent writing on how Japanese people read their comic books and how perhaps they should read them. As his essay title suggests, he feels the time is now to reconsider Taniguchi’s artistic achievement. In fact, a recent exhibition of Taniguchi’s art ran again in his home prefecture, Tottori, from last January through February. His work continues to be popular with Japanese, such as The Solitary Gourmet (Kodoku no gurume, 1994-96; 2008-2015), which was adapted into a popular television and web series. (English-speaking audiences need only to wait until next year for the translated edition from Fanfare/Ponent Mon.)
- Jon Holt & Teppei Fukuda, translator
Aquatic animals caught by a small set net from Hibiki-nada Sea off Yamaguchi Prefecture, Japan
No full textAquatic animals fished by 297 operations of a set net at the Hibiki-nada Sea off Yamaguchi Prefecture, Japan, were investigated in all seasons from 2002 through 2010. A total of 410 species including 3 reptiles, 1 chordate, 294 fishes (FS) and 112 invertebrates (IV) were caught. Among them, 242 FS and 100 IV were judged as rare (caught in less than 60 times), 28 FS and 8 IV as occasional (60 to 118 times), 9 FS and 2 IV as often (119 to 178 times), 8 FS and 1 IV as frequent (179 to 237 times), and 7 FS and 1 IV as general (more than 237 times) species. One hundred and one (101) FS and 29 IV were caught in all seasons, 170 FS and 78 IV were in spring, 201 FS and 77 IV were in summer, 207 FS and 55 IV were in autumn, and 185 FS and 64 IV were in winter. From distributional point of view, 118 FS and 43 IV were subtropical species, 108 FS and 60 IV were temperate region species, and 68 FS and 9 IV were large areal (widely distributed) species.departmental bulletin pape
Aquatic animals caughy by a small beam trawl net from Hibiki-nada Sea off Yamaguchi Prefecture, Japan
No full textAquatic animals fished by 37 operations of a small beam trawl net at the Hibiki-nada Sea off Yamaguchi Prefecture, Japan, were surveyed in all seasons from 2002 through 2009. A total of 317 species including 1 chordate, 204 fishes(FS) and 112 invertebrates(IV) were caught. Among them, 126 FS and 75 IV were judged as rare(caught in less than 8 operations), 36 FS and 28 IV as occasional(8 to 14 operations), 21 FS and 8 IV as often(15 to 22 operations), 16 FS and 1 IV as frequent(23 to 29 operations), and 5 FS and O IV as general(more than 29 operations). Sixty-eight FS and 28 IV were caught in all seasons, 107 FS and 71 IV were in spring, 139 FS and 79 IV were in summer, 148 FS and 81 IV were in autumn, and 125 FS and 43 IV were in winter. From distributional point of view, 70 FS and 42 IV were subtropical species, 91 FS and 62 IV were temperate region species, and 43 FS and 7 IV were large areal species.departmental bulletin pape
Career decision-making as dynamic semiosis: Autoethnographic trajectory equifinality modeling
No full textThis study aims to describe and analyze the career decision-making of the first author in the context of life trajectory using autoethnographic trajectory equifinality modeling (Auto-TEM). The life story and analysis of the first author indicate that career decision-making is a constant pre-constructive process in the life trajectory of an individual. This process is a semiotic and trans-action one between the individual and society. In other words, career decision-making is an issue of the cultural psychology of dynamic semiosis, which is concerned with understanding the manner in which the life trajectory of a person promotes or inhibits socio-culturalism and not only a subject of matching and self-esteem. Alternatively, we emphasize that career decision-making for an individual is an act of meaning with a unique ontological aspect. We propose the value of Auto-TEM as a qualitative method for describing such a dynamic career decision-making process. The autoethnographic viewpoint renders possible the understandings of the dynamics of the personal–collective culture synthesis for the subject. © The Author(s) 2022.journal articl
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