706 research outputs found
Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties
No full textAdditional file 4. Position and distribution of methylated and unmethylated cytosines for the cry1A.105 gene sequence in all analysed hybrids. Each line corresponds to one sequenced bacteria colony (clone). Clones from the same hybrid contain the same nomenclature, ranging from 1â24. Class I: CG residue; Class II: CHG residue; Class III: CHH residue. Filled icons: methylated cytosine; Empty icons: non-methylated cytosines
Investigations of immunogenic, allergenic and adjuvant properties of Cry1Ab protein after intragastric exposure in a food allergy model in mice
No full textBackground
In genetically modified (GM) crops there is a risk that the inserted genes may introduce new allergens and/or adjuvants into the food and feed chain. The MON810 maize, expressing the insecticidal Cry1Ab toxin, is grown in many countries worldwide. In animal models, intranasal and intraperitoneal immunisations with the purified Cry1Ab proteins have induced immune responses, and feeding trials with Cry1Ab-containing feed have revealed some altered immune responses. Previous investigations have primarily measured antibody responses to the protein, while investigations of clinical food allergy symptoms, or allergy promotion (adjuvant effect) associated with the Cry1Ab protein are largely missing. We aimed to investigate immunogenic, allergenic and adjuvant properties of purified Cry1Ab toxin (trypCry1Ab, i.e., trypsin activated Cry1Ab) in a mouse model of food allergy.
Method
Female C3H/HeJ mice were immunized by intragastric gavage of 10 μg purified, trypsin activated Cry1Ab toxin (trypCry1Ab) alone or together with the food allergen lupin. Cholera toxin was added as a positive control for adjuvant effect to break oral tolerance. Clinical symptoms (anaphylaxis) as well as humoral and cellular responses were assessed.
Results
In contrast to results from previous airway investigations, we observed no indication of immunogenic properties of trypCry1Ab protein after repeated intragastric exposures to one dose, with or without CT as adjuvant. Moreover, the results indicated that trypCry1Ab given by the intragastric route was not able to promote allergic responses or anaphylactic reactions against the co-administered allergen lupin at the given dose.
Conclusion
The study suggests no immunogenic, allergenic or adjuvant capacity of the given dose of trypCry1Ab protein after intragastric exposure of prime aged mic
Revisiting Risk Governance of GM Plants: The Need to Consider New and Emerging Gene-Editing Techniques
No full textNew and emerging gene-editing techniques make it possible to target specific genes in species with greater speed and specificity than previously possible. Of major relevance for plant breeding, regulators and scientists are discussing how to regulate products developed using these gene-editing techniques. Such discussions include whether to adopt or adapt the current framework for GMO risk governance in evaluating the impacts of gene-edited plants, and derived products, on the environment, human and animal health and society. Product classification or definition is one of several aspects of the current framework being criticized. Further, knowledge gaps related to risk assessments of gene-edited organisms—for example of target and off-target effects of intervention in plant genomes—are also of concern. Resolving these and related aspects of the current framework will involve addressing many subjective, value-laden positions, for example how to specify protection goals through ecosystem service approaches. A process informed by responsible research and innovation practices, involving a broader community of people, organizations, experts, and interest groups, could help scientists, regulators, and other stakeholders address these complex, value-laden concerns related to gene-editing of plants with and for society
Molecular responses of genetically modified maize to abiotic stresses as determined through proteomic and metabolomic analyses.
No full textSome genetically modified (GM) plants have transgenes that confer tolerance to abiotic stressors. Meanwhile, other transgenes may interact with abiotic stressors, causing pleiotropic effects that will affect the plant physiology. Thus, physiological alteration might have an impact on the product safety. However, routine risk assessment (RA) analyses do not evaluate the response of GM plants exposed to different environmental conditions. Therefore, we here present a proteome profile of herbicide-tolerant maize, including the levels of phytohormones and related compounds, compared to its near-isogenic non-GM variety under drought and herbicide stresses. Twenty differentially abundant proteins were detected between GM and non-GM hybrids under different water deficiency conditions and herbicide sprays. Pathway enrichment analysis showed that most of these proteins are assigned to energetic/carbohydrate metabolic processes. Among phytohormones and related compounds, different levels of ABA, CA, JA, MeJA and SA were detected in the maize varieties and stress conditions analysed. In pathway and proteome analyses, environment was found to be the major source of variation followed by the genetic transformation factor. Nonetheless, differences were detected in the levels of JA, MeJA and CA and in the abundance of 11 proteins when comparing the GM plant and its non-GM near-isogenic variety under the same environmental conditions. Thus, these findings do support molecular studies in GM plants Risk Assessment analyses
1981:2
No full textARTIKLAR
Alf Härdelin: Liturgi och litteratur
Antoon Geels: Religionspsykologi i Holland. Religiös varseblivning och upplevelse
Gunnar Wikmark: Carl Jonas Love Almquists präst- och pastoralexamina. Kring ett biblioteksfynd
LITTERATUR
G. W. Andersson (red.): Tradition and Interpretation. Essays by Members of the Society for Old Testament Study. Anm. av Stig Norin
Brevard S. Childs: Introduction to the Old Testament as Scripture. Anm. av Fredrik Lindström
Frederick Neumann: Where Do We Stand? A Selective Homiletical Commentary on the Old Testament. Volume III. The Psalms and Wisdom Literature. Anm. av Sam. Nyström
Benkt-Erik Benktson: ADAM – VEM ÄR DU? Typologi och teologi. En probleminventering. Anm. av Tage Kurtén
Helmut Thielicke: Der evangelische Glaube. Grundzüge der Dogmatik. III. Band. Anm. av Agne Nordlander
Ulrich Nembach (red.): Begründungen des Rechts. Anm. av Jarl Hemberg
Bruno Schüller: Die Begründung sittlicher Urteile. Typen ethischer Argum entation in der Moraltheologie. Anm. av Jarl Hemberg
Uppsala stifts herdaminne. Stiftshistoriskt uppslagsverk från reformationen till nyaste tid. Anm. av Ingmar Brohed
Kungl. Humanistiska Vetenskapssamfundet i Lund. Scripta minora 1978–1979 in memoriam Gustavi Aulén. Anm. av Göran Bexell
Felix Klein-Franke: Die klassische Antike in der Tradition des Islam. Erträge der Forschung. Anm. av Jan Hjärp
IN MEMORIAM
Harald Riesenfeld: Olof Linton 1898–198
The Gunnar Ekelöf Room and the Poet’s Widow as Archivist and Author
No full textThe Gunnar Ekelöf Room is a reconstructed memorial museum of the late home of the Swedish modernist poet at the Sigtuna Foundation not far from Stockholm. While Gunnar Ekelöf’s original manuscripts are archived at Uppsala University Library, their copies are accessible in the duplicate so-called Home Archive, set up by his widow Ingrid Ekelöf and housed in the Gunnar Ekelöf Room, as is also the extensive correspondence between her and the literary critic Brita Wigforss. Guided by cultural memory studies and archival studies which regard archives and writers’ houses as texts and media, this chapter explores how the Home Archive through this correspondence recounts its own origin, thereby offering new aspects of the metonymic principles that generally guide archival work.</p
"Thou Shalt Make No Graven Maps!": An Interview with Gunnar Olsson
No full textThe author spoke with renowned Swedish geographer Gunnar Olsson about maps, GIS and the power of imagination in both history and geography
Re:Reading piece on author Gunnar Hansen of Northeast Harbor. Hansen wrote I
No full textRe:Reading piece on author Gunnar Hansen of Northeast Harbor. Hansen wrote Islands at the Edge of Time, and recently worked on the screenplay for a documentary produced by the Penobscot Nation
Landshövding Wilhelm Stråles promemoria om C. J. L. Almqvist. Till diskussionen om den Almqvistska gåtan [Elektronisk resurs]
No full textAcquisition of foreign DNA by natural transformation in Acinetobacter baylyi: quantitative estimates and molecular characterization of the introgression process
No full textVery little is known about introgression in bacteria. Introgression is the process where the genes of one species infiltrate the gene pool of another organism by subsequent backcross transformations of a hybrid with one of its parents. After the initial acquisition of foreign DNA, DNA from the newly made transformants is used as donor DNA in backcross transformations with the recipient. DNA is released to the environment after decomposing of dead cells, disrupting of cells or through excretion from living organisms. The extracellular DNA can be degraded after release, thus fragmented DNA can be taken up by bacteria.
One previous unpublished study investigated the effect of introgression of foreign unselected DNA. The donor DNA in this study was of high molecular weight (20 to 30 kilo bases (Kb)) and it was suggested that introgression in backcross transformation could be a mechanism by which unselected DNA was eliminated from the genome.
I wanted to study the effect of introgression when the foreign donor DNA was of low molecular weight (1000 to 4000 base pairs (bp)). We wanted to determine how fragmentation affects the speed at which unselected DNA from Acinetobacter sp. strain 16.4 was eliminated from the genome of Acinetobacter baylyi strain BD413 during the introgression process.
I have developed a method for optimal fragmentation of DNA to the desired size for this study. The DNA was fragmented by sonication, which gave an effective, gradual reduction in the fragment size of DNA. The size of the sonicated DNA was checked on an agarose gel and I found out that a gel fraction between 1000 to 4000 bp was the desired size for fragmented DNA. The DNA was extracted from the gel piece and used as low molecular weight donor DNA.
This method can be used to determine the effect of introgression when the foreign donor DNA is of low molecular weight (1000 to 4000 base pairs bp) and to get a better understanding for natural fragmentation of extracellular DNA in the environment
- …
