30 research outputs found
EKSTRAK ETANOL TERIPANG PASIR (Holothuria scabra) DALAM MEMPERBAIKI KERUSAKAN USUS HALUS MENCIT (Mus Musculus) YANG TERPAPAR KALIUM BROMAT MELALUI PENGAMATAN GAMBARAN HISTOPATOLOGI MENCIT
No full textBackground: In Indonesia, traditionally sea cucumber (Holothuria scabra) is often used as a drug for uric acid. A study has been conducted on the effect of giving sandy cucumber extract (Holothuria scabra) to microscopic images of the small intestine of mice (Mus muculus) induced by potassium bromate.
Method: This study used a small intestine sample of 2-3 months old male mice weighing + 20-30gr for 15 mice used in the study, divided by 5 groups, ie group I was given allopurinol as a positive control, group II was given aquades as control group negative, group III, IV and V were given extract of sea cucumber sand and potassium bromate as treatment group with concentration of 5%, 10%, and 15%.
Result: The result of the research was analyzed quantitatively, showed that the extract of sea cucumber with 10% concentration was not significantly different with the extract of sea cucumber with concentration of 15%, when compared with the giving of sea cucumber extract with 5% concentration gave significantly different result with the giving of extract sea ​​cucumbers with concentrations of 10% and 15%. This shows the provision of sea cucumber extract with a concentration of 5% can give microscopic changes in the small intestine of mice due to exposure to potassium bromate.
Conclusion: Sand cucumber extract (Holothuria scabra) with 5% concentration can give a change of histology picture of small intestine of mice (Mus muculus)
PAPARAN EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP GAMBARAN HISTOPATOLOGI HATI MENCIT (Mus musculus)
No full textBackground: In Indonesia, traditionally sea cucumber (Holothuria scabra) is often used as a cure gout. The purpose of the research is to determine the effects of sea cucumber (Holothuria scabra) extracts against liver histopathology picture mice (Mus musculus).
Method: Mice used were male mice as much as 15 tails and were divided into 5 groups. Each group consisted of three mice. Group I as a positive control given allopurino l0.1%. The second group was given distilled water as a negative control. Group III, IV and V as the treatment group was given sea cucumber extract with each concentration of 5%, a concentration of 10% and 15% concentration.
Result: The results were analyzed quantitatively, showed that the administration of potassium bromate showed the presence of fatty and necrosis. But after being given a sea cucumber extract with a concentration of 10% and a concentration of 15% still showed the presence of inflammatory cells and remnants of fatty liver (same as the picture of liver histopathology in the positive control). At a concentration of 5% normal liver histopathology same as the picture of liver histopathology in the negative control.
Conclution: Sea cucumbers with a concentration of 5% can be used as a gout medicine without any side effects for conditions onal liver function
STUDI PEMANFAATAN TUMBUHAN OBAT TRADISIONAL OLEH MASYARAKAT SUKU OIRATA PULAU KISAR KECAMATAN PULAU-PULAU TERSELATAN KABUPATEN MALUKU BARAT DAYA
No full textBackground: Medicinal plants are medicinal plants that can relieve pain, increase endurance and treat various diseases. The Oirata tribe is an indigenous tribe of Kisar Island. The community traditionally uses various types of plants as alternative treatments to cure diseases because they are easier to obtain and there are no side effects.
Methods: This study uses descriptive methods. Data collection techniques by conducting interviews, observation, questionnaires, and collecting and documenting medicinal plants.
Results: Medicinal plants found in the Oirata Tribe of Kisar Island, the Sub district Islands, Southwest Maluku Regency are 31 types of medicinal plants. Plant parts used are roots, bark, leaves, flowers, fruits and seeds. How to process traditional medicinal plants is done by boiling, pounding, chopping, squeezing, pasting, wrapping, heating, and some even eaten directly to swallow the water.
Conclusion: The way to use it for the people of the Oirata tribe is that it is single because they do not use plants mixed with plants or other raw materials only mixed with water
EFEKTIVITAS EKSTRAK BIJI PANGI (Pangium edule Reinw) TERHADAP MORTALITAS LARVA NYAMUK Anopheles
No full textBackground: Eradication of mosquito nests in Maluku is still done by fogging and with other chemicals. But on the other hand can cause disease in the respiratory tract and environmental damage. Pangi seeds contain alkaloids and potentially saponin compounds as larvacide to kill mosquito larvae.
Method: The study design was using RAL (Completely Randomized Design) with 5 treatments ie K1 (control), K2 0,04%, K3 0,08%, K4 0,16% and K5 0,23% with each 25 larvae with 3 repetitions observed for 24 hours. One Way ANOVA Test Result and Probit LC50 Analysis were used to find out the extract of pangi seed which is effective in killing mosquitoes.
Result: One Way ANOVA test result obtained Fcount value is 467.063 with Sig value. 0.000 and probit analysis obtained 24 hours LC50 value of 0.053% means that the extract of pangi seed (Pangium edule Reinw.) Is effective against mortality of Anopheles mosquito larvae.
Conclusion: Seed extract (Pangium edule Reinw) effect on mortality of Anopheles mosquito larvae with LC50 extract value of Pangi (Pangium edule Reinw) in 24 hours was 0.053%. Each concentration of pangi seed extract (Pangium edule Reinw) had an effect on mortality of Anopheles mosquito larvae within 24 hours of observation time. There was a correlation between various concentrations of Pangi (Pangium edule Reinw) with the mortality of Anopheles mosquito larvae with value (p) = 0.000 and the relationship level was very strong with Pearson correlation value 0.891
ANALISIS SENYAWA FLAVONOID DAUN LAMUN Enhalus acoroides DI PERAIRAN PANTAI DESA WAAI KABUPATEN MALUKU TENGAH
No full textBackground: The coastal waters of Waai Village are the waters located in Kecamatan Kecamatan, Central Maluku District. The potential of coastal resources in this area is quite a lot of seagrass (Seagrass). Although quite a lot but because of the lack of information related to the existing seagrass so that until now the utilization of seagrass in the area has not been maximized. This study aims to determine the total flavonoid compounds contained in the seagrass of Enhalus acoroides.
Method: This research was conducted at Basic Chemical Laboratory, Faculty of Mathematics and Natural Sciences (FMIPA) Pattimura University Ambon. The study was conducted from March 20-28, 2016, with sampling location in coastal waters of Waai Village, Central Maluku District. The method used refers to the Chang method by using AlCl3 reagent.
Result: Based on total analysis of flavonoid compound using laboratory experimental method obtained the average flavonoid level contained in Enhalus acoroides seagrass leaves of 3.569%.
Conclusion: Seaweed leaves Enhalus acoroides taken from coastal waters of Waai Village contain flavonoid compounds with an average value of 3.5697%
ANALISIS KANDUNGAN FLAVONOID PADA TEH BENALU (Dendropohtoe pentandra (L.) Miq.)
No full textBackground: Benalu is one of the plant species that lives attached to other plants. The parasite is hemiparasit or half parasitic because it has a green leaf substance (chlorophyll) used for the assimilation process and only sucks water and organic matter from its host plant. Flavonoids are phenol compounds commonly found in vascular plants including parasites. Flavonoids are useful for protecting cell structures, increasing the effectiveness of vitamin C, anti-inflammation, preventing bone loss and as an antibiotic.
Method: Total flavonoid content in clove parasite tea using UV-Vis Spectrophotometer method.
Results: Total flavonoid in tea clove parasite was 0.2819%. Flavonoids found in tea parasite cloves are low with the presence of heating and temperature factors.
Conclusion: Based on the quantitative test, leaf parasite is the highest flavonoid level of 13.702% and after being made tea, flavonoid levels changed to 0.281%
UJI DAYA HAMBAT EKSTRAK ETANOL SURUHAN (Piperumia pellucida L.H.B Kunth) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN-VITRO
No full textBackground: Staphyloccocus aureus represent normal mikroflora of human being. This bacterium usually there are at bronchi to the and husk. Existence of Staphyloccocus aurues at bronchi to the and husk at individual seldom cause disease, healthy individual usually only personating career. One of the original plant that is plant of suruhan (Piperumia pellucida L.H.B. Kunth) biggest and wide of in some area in Indonesia (Molluca) and also have potency to be developed as traditional drug, empirically have been used in medication of abscess (abses) by society.
Methods: This Research use eksperimental in Laboratory with research device is Complete Random Device (Factorial RAL) Pattern from two factor and 3 restating, which in doing/conducting in Elementary Laboratory of Biology Faculty Teachership and Science Education of Pattimura University.
Resulth: Result research of to extract etanol plant suruhan (Piperumia pelludica L.H.B Kunth) to growth of bacterium of Staphylococcus aureus with various concentration that is 25%, 50% and 75% and there is influence contact to growth of bacterium where at concentration 25% is equal to 5 mm donot give respon pursue, 50% is equal to 10 mm with weak resistance respon and 75% is equal to 16 with resistance respon.
Conclusion: Extract concentration excelsior of etanol plant of suruhan (Piperumia pelludica L.H.B Kunth) and old progressively of contact with bacterium of Staphylococcus aureus hence resistivity progressively goodnes
ANALISIS KADAR KARBOHIDRAT DAN LEMAK PADA TEMPE BERBAHAN DASAR BIJI LAMUN (Enhalus acoroides)
No full textBackground: Seagrass (Enhalus Acoroides) is one of the most widely spread seagrass species in Indonesia and the seeds can be used by the community to be semi-processed product like Tempe (Fermented soyben). Another advantage possessed by Seagrass (Enhalus Acoroides) is that it has quite high nutrition. This research aimed to find out the carbohydrate and fat level on Tempe made from the fermentation of the seagrass seeds (Enhalus acoroides).
Method: The analysis of the level of carbohydrate used by different method while the fat used Soxhlet method.
Result: Based on the result of the research if the seagrass seeds been processed to be tempe, thus the carbohydrate and fat level was increased with the comparison of appropriate yeast. Conclusion: raw seagrass seeds if being processed to be tempe can increase the level of nutrition with the ppropriate amount of the yeast compared to consuming directly
Inhibition of methanol extract snail gonggong (Strombus urturella) to pathogenic bacteria Vibrio alginolyticus
No full textThe purpose of this study was to determine the methanol extract of snail gonggong (Strombus turturella) and the inhibition of methanol extract of gonggong snail meat (Strombus turturella) against the pathogenic bacterium Vibrio alginolyticus. The study used a completely randomized design. The treatment was giving the extract of snail gonggong meat with concentration of 100%, 50%, 25%, positive control using amoxcillin and negative control using distilled water with three replications. The identification of bioactive compounds included alkaloids, saponins, flavonoids, steroids and triterpenoids. The antibacterial activity of the methanol extract of snail gonggong meat (Strombus turturella) was tested by the diffusion method at concentrations of 100%, 50% and 25%. The data collected is then analyzed descriptively qualitatively. The results showed that the antibacterial activity of the 100% concentration of the methanol extract of the gonggong snail meat had moderate inhibition against Vibrio alginolyticus (diameter 7,833 mm). while the concentrations of 50% and 25% did not have an inhibition zone. The identified bioactive compounds included alkaloids, saponins, flavonoids and steroids, while the terpenoid and phenolic compounds were not identified. Thus, the methanol extract of gonggong snail meat has antibacterial potential that can be used to overcome the attack of pathogenic bacteria
