1,398 research outputs found
David Walker collection, 1834-1879
No full textThis collection contains correspondence from prominent Arkansans to David Walker, and other miscellaneous documents.; Correspondents include: Benjamin F. Danley, C. C. Danley, Thomas Drew, Elbert English, W. B. Flippen, Absalom Fowler, Benjamin Johnson, Augustus Garland, William R. Miller, James Mitchell, Isaac Murphy, Henry Rector, Logan Roots, R. W. Trimble, George C. Watkins, A. J. Wilson, William Woodruff, and Archibald Yell. The correspondence concerns legal, financial, and political matters from the 1830s until the 1870s, including secession, the Civil War, and the Brooks-Baxter War.; This collection was originally numbered H-12 and is part of the J. N. Heiskell Historical Collection, courtesy Arkansas Gazette Foundation.David Walker collection, 1834-187
D-1481: 312 North 500 East, Logan, Utah, David R. and Janice Walker residence. Lot 2 Block 11 Plat C
No full textD-1481: 312 North 500 East, Logan, Utah, David R. and Janice Walker residence. Lot 2 Block 11 Plat
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Comparison of Hamiltonian structure-preserving and Floquét mode station-keeping for Libration-point orbits
No full textLibration-point orbit (LPO) missions are often selected for study the Sun and our Universe. The effect of perturbations such as the gravitational effect of other planets and Solar Radiation Pressure (SRP) makes the LPO's environment highly unstable. Thus, station-keeping manoeuvre are required to keep the spacecraft close to its nominal trajectory and a novel idea is to use perturbations such as SRP for a propellant-free control system. A Hamiltonian-Structure Preserving (HSP) control, which exploits the natural dynamics, is selected since it requires low acceleration. The focus of this paper is to investigate the robustness of HSP; thus, a comparison with a similar approach such as the Floquét Mode (FM) is analysed to identify their main features. A sensitivity analysis by changing the HSP and FM controllers' parameter is done for SOHO's mission; while, an extension of the HSP control enhanced with SRP is investigated for high amplitude planar-Lyapunov and distant prograde orbits
Methods for Modulation of Surface Calreticulin and Subsequent Phagocytosis
No full textAn increase in surface calreticulin (CRT) can promote recognition of cells by macrophages and the immunogenicity of cell death, and is a desirable response in current chemotherapeutics. Using standard hematopoietic cell lines, Jurkat, THP-1 and HL-60, a range of small molecules postulated to increase surface CRT were tested. Overall, raphin1, sephin1, nelfinavir and ritonavir showed promise for increasing surface expression of CRT, which led to an increase in both THP-1 and RAW 263.7 mediated phagocytosis with raphin1 and ritonavir. A second method of increasing surface CRT was also explored. THP-1 pre-treated with P-3Fax-Neu5Ac were found to have a reduction in terminal α2, 3 and α2, 6 N-acetylneuraminic acids, exposing an obscured Tri/ Mii glycan, which allowed for increased binding of exogenous CRT, and subsequent phagocytic uptake by differentiated THP-1 macrophage-like cells.M.Sc.2023-06-28 00:00:0
Nanopore Sequencing of RNA from Breast Cancer Genes
No full textAbnormal mRNA splicing can disrupt gene function and influence the course of disease. Analysis of abnormal splicing is an important part of determining whether a particular genetic variant found in the population is pathogenic or not. However, to correctly identify abnormal splicing, we must first understand what is normal. This project assessed the isoforms of the genes BRCA1 and BARD1, which are particularly relevant to the onset of breast cancer.
BRCA1 is a tumour suppressor gene implicated in breast cancer onset. BARD1 codes for a protein that interacts with BRCA1 and produces a smaller mRNA transcript. Normal exon skipping events have been identified for both BRCA1 and BARD1, however, current methods are unable to reliably identify full transcripts. This has resulted in knowledge of individual exon skipping events but often does not tell us whether multiple events occur in the same transcript. The MinION nanopore sequencer (Oxford Nanopore Technologies), uses a nanopore to produce long-read, single molecule sequences. This has great potential for identifying multiple long isoforms, which is not practical using current technologies. The aim of this project was to examine the ability of the MinION to identify mRNA splicing patterns of transcripts derived from BRCA1 and BARD1.
All mRNA from a normal lymphoblastoid cell line was converted to cDNA and targeted genes of interest were amplified by polymerase chain reaction (PCR). All potential isoforms generated from BRCA1 and BARD1 were then pooled and analysed using the MinION sequencer. After trialling many different analysis methods, the read data was analysed using the BLAST-like Alignment Tool (BLAT) with two outputs, a tabular and a graphical format. The tabular format grouped reads into potential isoforms, while the graphical format allowed visualisation of these isoforms and identified the exon/intron boundaries. Using both these formats 34 BRCA1 isoforms and 39 BARD1 isoforms were identified, 24 and 17 of which were potential novel isoforms respectively. Two of these novel isoforms from the BRCA1 dataset (Δ10-17 and Δ11q21) were further verified using Sanger sequencing.
This was a proof of principle research project that demonstrated the potential use of the MinION nanopore sequencer for successful characterisation of multiple mRNA isoforms. This research has successfully identified a number of novel isoforms from the BRCA1 and BARD1 genes using the MinION sequencing device
Evaluating molecular markers of breast tumour progression
No full textBackground: Tumour cell proliferation has emerged as a major prognostic factor in breast cancer. The immunohistochemical (IHC) proliferation marker Ki67 has been extensively investigated, but has not gained widespread clinical acceptance. Phosphohistone H3 (PHH3) is a new immunohistochemical marker for quantifying mitoses, however there is limited information on its prognostic value in breast cancer. This study performed a head-to-head comparison of Ki67 and phosphohistone H3. The aims were to establish the marker of greatest prognostic value, and to compare Ki67 protein expression and Ki67 mRNA levels in breast tumours.
Methods: Tumour samples from 108 women with breast cancer were constructed as tissue microarrays (TMAs) and evaluated using immunohistochemistry. Cox proportional hazards regression was used to evaluate the prognostic significance of Ki67 and phosphohistone H3. C-statistics, integrated discrimination improvement (IDI), and net reclassification improvement (NRI) were used to evaluate the discriminatory ability of various prediction models. Ki67 mRNA from 30 matched fresh/frozen tumour specimens was analysed using quantitative polymerase chain reaction (qPCR), and was compared with Ki67 protein expression.
Results: Phosphohistone H3 had greater prognostic value than Ki67 in a multivariable model that adjusted for traditional prognostic variables in breast cancer (hazard ratio: 3.32; 95\% confidence interval: 1.51--7.30). A risk prediction model that incorporated phosphohistone H3 efficiently separated patients at low risk and high risk of death at 5-years after diagnosis. The correlation between Ki67 protein expression and Ki67 mRNA levels was weak.
Conclusions: Phosphohistone H3 outperforms Ki67 as an independent predictor of survival in breast cancer. Phosphohistone H3 may provide important information for counselling patients about the likely outcome of their disease, and accurately classifying groups of patients for clinical trials
'The Wyandots: Being the History of an American Indian Nation Whose Warriors Never Surrendered'
No full textComplete history of the Wyandot Indians written by Don C. Seitz of Fairfield County, Ohio. This typed draft with corrections includes a glossary of Wyandot language (p. 265-270) compiled by N. O. Walker and Mary McKee, and a muster roll of all Wyandot Indians who emigrated west of the Mississippi River in July 1843 (p. 359-361). Chapters include "The Nation," "The Sandusky Country," "The Turmoil,"Dunmore, Logan, Cornstalk," "The Moravians," "The Massacre of the Christians," "Battle Island," "Tymochte," "Escape of Knight and Slover," "Unsettled Relationships," "The Defeats of Harmar and St. Clair," "Efforts at Peace," "Wayne's Victory," "Boone, Kenton, Poe," "Tecumseh and the Prophet," "Eighteen Hundred and Twelve," "The Missions," "The Pressure," "The Migration," "Life in the West," "Kansas-Nebraska," "The Canadian Remnant," "The White Indians," "Government and Society," "Traditions," and "Characteristics.
Effects of Environmental Water Transfers on Stream Temperatures
No full textLow streamflows and warm stream temperatures, caused mainly from agricultural diversions, currently limit available habitat and productivity of trout, including native Lahontan cutthroat trout in Nevada’s Walker River Basin. Environmental water purchases, which transfer water from willing sellers to instream uses (i.e for fish), are being evaluated to improve instream habitat. To determine which environmental water purchases to prioritize, this study was undertaken to build a computer model in order to simulate stream temperatures under differing environmental water transfer scenarios. Model runs simulate a range of environmental water transfers at major diversions and reservoirs throughout the Walker River Basin. Results indicate that low flows generally coincide with critically warm stream temperatures, cooler stream temperatures exist in the East Walker River, a tributary of the Walker River, during warm seasons which provide good habitat for fish, environmental transfers can improve stream temperatures for some highly impacted reaches by up to 3°C in dry years, and environmental water transfers have a greater effect in dry years than wet years
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