183 research outputs found
Phytochemical and Pharmacological Activities of Curcuma purpurascens Blume, A Review
Get PDFCurcuma sp. is generally used for medicine, starch sources, preservatives, dyes and cosmetics. The use of Curcuma spp. for medical has increased because there have been many studies related to its active ingredients, such as flavonoids, essential oils, tannins, quinones, and terpenoids, as well as pharmacological activities, including wound healing, antioxidants, antifungal, anticancer, gastroprotective, and hepatoprotective. Curcuma purpurascens Blume is a species of Curcuma from family Zingiberaceae and used for traditional medicine. This article focuses on reviewing the literatures on C. purpurascens and discussing its morphology, phytochemical content, and pharmacological aspects. The method used to review this article was by exploring several databases such as Scopus, Pub Med, and Google Scholar to identify and download original articles and research journals related to the morphology, phytochemical content, and biological activity of Curcuma purpurascens Blume. The result of this review will later provide information about the uses and presence of Curcuma purpurascens Blume which is still rarely studied so further study related to its pharmacological activity tests and active compound as natural medicines can be explored
Sitotoksisitas rimpang temu mangga (Curcuma Mangga Val. & V. Zijp.) dan kunir putih (Curcuma Zedoaria L) terhadap beberapa sel kanker manusia (in vitro) dengan metoda SRB
Get PDFABSTRACT
Mae Sri Hartati W, Sofia Mubarika, R. L. H. Bolhuis, K. Nooter, R. G. Oostrum, A. W. M. Boersma, Subagus Wahyuono - Cytotoxic effect on human cancer cell using SRB method (in vitro) rimpang temu mangga (Curcuma Mangga Val. & V. Zijp.) dan kunir putih (Curcuma Zedoaria I.).
Background: Temu mangga (Curcuma mangga) and kunir putih (Curcuma zedoaria) rhizomes have been utilized by people to treat cancer lately although clinically their activity has not been tested. Objectives: This study is aimed to prove their activity as anticancer on 7 major human cancers cell lines (in vitro), and to evaluate whether these two rhizomes are potential for new anticancer drug. Methods: The two rhizomes were separately extracted with chloroform followed by methanol to give chloroform extracts of C. mangga (CmCh) and C. zedoaria (CzCh)and methanol extracts of C. mangga (CmMe) and C. zedoaria (CzMe) respectively. These extracts were tested their cytotoxic effect on 7 major human cancer cell lines using SRB method (in vitro). Doxorubicin and cisplatin were used as positive controls, and their cytotoxic effects were measured by comparing their ID50 with that of the positive controls.
Results: Those four extracts (CmCh, CzCh, CmMe and CzMe) practically did not perform cytotoxic effect on those human cancer cell lines, because the extract\u27s ID50 was far higher than the positive controls on those human, cancers cell lines
Conclusion: The C. mangga and C. zedoaria rhizomes did not active as anticancer and they were not pOtential as the source of a new anticancer drug.
Key words: anticancer - Curcuma mangga - Curcuma zedoaria - cytotoxic - drug discover
Marine Sponge Jaspis Sp, A Potential Bioactive Natural Source Against Infectious Diseases
Get PDFPuji Astuti, Sylvia Utami Tunjung Pratiwi, Triana Hertiani, Gemini Alam, Akbar Tahir, Subagus Wahyuono - Marine Sponge Jaspis sp, A Potential Bioactive Natural Source against Infectious diseases
Background: The high incidence of microbial infection and the emergence of drug resistant and multidrugresistant microbes as well as the lack of any current chemotherapy augmented the necessity to search for new and better antimicrobial drug. Marine invertebrates are known as rich sources of compounds with unique chemical structures and pronounced chemical and biological activities, which suggests potential value as lead structures for the development of new pharmaceuticals.
Objective: This study aims to screen potential antiinfective of sponges extracts collected from Barrang Lompo island and report on their antibacterial and antifungal properties.
Methods: Testing for anti-infective agents was conducted using dilution method. Nutrient Agar was used as the testing media and nutrient broth for the inoculation of microorganisms. Staphylococcus aureus, Escherichia coil and Salmonella thypi were used as the testing bacteria and Candida albicans for the testing fungi. Chloramphenicol was used as positive control for antibacterial testing and ketocozaole for antifungal testing.
Results: From the 11 acetone extracts tested, BL-02, BL-09, BL-10 and BL-12 was found to inhibit the growth of microorganisms and the extract of BL-10 was found to be the most active. Bioautography results suggest that the polar fractions were responsible for the growth inhibition.
Conclusion: the polar fraction of acetone extract of BL-10 was considered to be potential compounds for further characterization as anti infective agents.
Key words: screening, sponge, antibacteria, antifung
Recommended from our members
PHYTOCHEMICAL INVESTIGATION OF AMSONIA GRANDIFLORA FAMILY APOCYNACEAE.
No full textIdentifikasi lupeol, asetil lupeol dan lupeil t3-hidroksioktadekanoat yang terdapat di dalam fraksi non-polar amsonia grandiflora(fam. Apocynaceae)
Get PDFABSTRAK
Sebagai kelanjutan penelitian mengenai kandungan kimia dari tanatnan Arnsonta grandiflora, telah diisolasi tiga senyawa dari sari non polar dari sari diklormetana tanaman tersebut. Ketiga senyawa tersebut merupakan senyawa triterpena yang mempunyai kerangka lupan dan diidentifikasi sebagai Lupeol, asetil lupeol dan lupeil /1-hidrolcsioktadekanoat. Identifikasi senyawa didasarkan atas data spektra ms, nmr), don perbandingan langsung dengan lupeol baku.
Kata kunci : Amsonia grandiflora, Apocynaceae, lupeol, asetil lupeol dan lupeil Ahidroksioktadekanoate
ABSTRACT
A phytochemical investigation on the non-polar fraction of Amsonia grandiflora has been done. A triterpene compound of lupane skeleton and its derivatives were isolated. These compounds were identified as lupeol, acetyl lupeol and lupeol /3-hydroxyoctadecanoate based on their chemical and physical data (ir, ms, nmr), and direct comparison with authentic lupeol.
Key words: Amsonia grandiflora, Apocynaceae, lupeol, acetyl lupeol and lupeol ft-hydroxyoctadecanoat
Recommended from our members
Potential anti-infective agents isolated from Artemisia pacifica Nutt and Guardiola platyphylla Gray (fam. Asteraceae).
No full textThe dichloromethane extracts (1 mg/ml) of Artemisia pacifica Nutt and Guardiola platyphylla Gray (fam. Asteraceae) separately demonstrated in vitro growth inhibition of Staphylococcus aureus (UA 9-29), Bacillus subtilis (UA 2-27), Klebsiella pneumoniae (UA 3-9) and Candida albicans (UA 97). Each of these extracts were subjected to bioassay-directed solvent extraction and partition in order to obtain concentrated active fractions. Subsequently, the active compounds were isolated and identified from these fractions. Artemisia pacifica Nutt. The active compound was the major component isolated from A. pacifica. By comparing the physical and chemical data with previously reported data, this compound was identified as dehydrofalcarindiol. Dehydrofalcarindiol demonstrated growth inhibition against S. aureus (50 μg/ml), B. subtilis (25 μg/ml), K. pneumoniae (100 μg/ml) and C. albicans (25 μg/ml). Its diacetyl derivative was devoid of activity at 100 μg/ml. Guardiola platyphylla Gray. The active fraction obtained from G. platyphylla contained unstable compounds that decomposed in the presence of air. Size exclusion chromatography (Sephadex LH-20) was used to fractionate the active fraction. Two new sesquiterpenes, the o-catechol derivatives (1S,4S) and (1S,4R)-7,8-dihydroxy-11,12-dehydrocalamenene, were eluted from the column as a mixture. The mixture of their diacetyl derivatives was oxidized with CrO₃ in AcOH. The major oxidation product was identified as (1S)-7,8-diacetyl-4-oxodeisopropylcalamenene, thereby verifying the sole difference to be the configuration at C-4. This sesquiterpene mixture completely inhibited the growth of S. aureus (100 μg/ml), B. subtilis (50 μg/ml), K. pneumoniae (100 μg/ml) and C. albicans (100 μg/ml). After removal of the sesquiterpenes from the active fraction, the remaining compounds displayed the same level of activity. Another six compounds were also isolated from this mixture as acetylated derivatives due to their instability. Their dimeric structures were identified by 2D-NMR techniques (COSY, HETCOR and NOESY). These dimers may be artifacts since they were formed from their o-quinone monomers when kept at room temperature for a week or when heated at 60°C for 4 hours.This item was digitized from a paper original and/or a microfilm copy. If you need
higher-resolution images for any content in this item, please contact us at
[email protected] file replaced with corrected file August 2023
Identifikasi dua asam triterpen yang terdapat di dalam amsonia grandiflora, Fam. Apocynaceae
Get PDFABSTRAK
Amsonia grandiflora adalah salah satu tanaman yang mudah tumbuh didaerah kering. Tanaman ini mengandung karbohidrat dalam jumlah besar disamping mempunyai nilai nutrisi yang sama dengan rumput alfalfa secara in-vitro. Tetapi tanaman ini tidak dimakan oleh ternak yang hidup didaerah tersebut. Dua senyawa asam triterpen dalam tanaman dapat diisolasi dart sari diklormetana A. grandiflora yang tidak larut dalam n-heksana. Berdasarkan alas spektrum inframerah, spektrum massa, spektrum resonansi magnetik inti, titik lebur dan rotasi oplik, identitikasi kedua senyawa tersebut mengarah kepada asam betulinat dan asam oleanolat. Perbandingan secara langsung kedua senyawa tersebut dengan senyawa asam betulinat dan asam oleanolat baku, maka kedua senyawa tersebut disimpulkan sebagai asam betulinat dan asam oleanolat. Keberadaan dua triterpene asam dalam jumlah relatif tinggi memungkinkan mempengaruhi rasa tanaman, yang memungkinkan ternak tidak mau memakannya.
Kata kuncl: Amsonia grandiflora, makanan ternak, asam betulinat, asam oleanolat.
ABSTRACT
An indigenous desert plant, Amsonia grandiflora, has a high carbohydrate content and an animal feed nutritive value in-vitro similar to alfalfahowever, this plant is not grazed in the wild. Two triterpene acids were isolated from the dichloromethane extract of A. grandiflora that was insoluble in n-hexane. Based on their infrared, mass and nuclear magnetic resonance spectra as well as their melting points and optical rotations, the two acids were suspected to be betulinic acid and oleanolic acid. Direct comparison of these two acids with authentic betulinic and oleanolic acid, these acids were confirmed as betulinic acid and oleanolic acid. The high concentrations of oleanolic acid and betulinic acid might impart a sour taste to the plant. This could possibly be the reason that animals in the wild do not grazed this plant.
Key words : Amsonia grandiflora, animal feedstocks, betulinic acid, oleanolic acid
Penetapan kadar kandungan utama simplisia kayu angin [Usnea misaminensis (Vain.) Not., lam. Usneaceae] dart pabrik jamu dan pasar secara densitometri = Quantitative assay of the main substance present in the kayu angin [
Get PDFABSTRACT:
Kayu angin (Usnea misaminensis (Vain.) Not., fain. Usneaceael adalah salah sate bahan obat tradisional di Indonesia yang banyak terdapat sebagai campuran dalam Jamu. Lokasi pengumpulan serta asal kayu angin untuk industry jamu sering kurang mendapat perhatian, sehingga menturtgldnkan latalitas bahan baku akan bervariasi.
Dalam penelitian ini telah dilakukan penetapan kadar senyawa utama yang terdapat dalam kayu angin lujau (KAH),Luning (KAK) dan kayu angin dari pabrik jam (KAJ) dan A\u27 (KAA) secara densitometri. Dalam penelitian ini, terlebih dahulu diisolasi senyawa utama dari kayu angin, kemudian dibuat kurwi baku (Y = 0,4224 X - 0,1108) antara saw deret kadar
senyawa (X) dengan data area/unit (Y) yang diperoleh dari densitometer. Kayu angin yang akan ditentukan kadarnya disari dengan aseton, dilakukan kromatografi lapis tipis dan ditentukan data area/unit-nya (Y) secara densitometri. Dengan memasuldcan harga Y pada persamaan diatas, maka kadar senyawa utama (X) dapat dihitung, yang kemudian dapat dikonversikan dalam prosentase senyawa utama datum kayu angin.
Kadar senyawa utama terbanyak terdapat pada kayu angin dari pabrik jamu (KAJZ14 ± 0,51 %) diikuti oleh kayu angin laming (KAK4,09 ± 0,27 %) dan kayu angin htfau (Ke1112,99 ± 0,41 %). Sedangkan kadar senyawa terkecil terdapat dalam kayu angin dart pabrik jamu \u27A\u27 (KAA1,57 + 0,33 %).
Kata kunci : Kayu angin (Usnea misaminensis (Pain.) Not.J, kandungan utama, densitometr
- …
