1,720,959 research outputs found
Localization of Phr1p in Candida albicans
No full textPHR1 is a pH-regulated gene belonging to a family of five genes of Candida albicans. PHR1 expression is triggered at pH values of the growth medium > 5.5. Loss of PHR1 induces morphological defects, inability to support hyphal growth and reduced virulence in animal mouse models of infection. Phr1p is endowed of a beta(1,3)-glucan elongase activity similarly to Gas1p of S.cerevisiae and Gel1-Gel2 proteins of A. fumigatus. In order to explore the function of Phr1p in Candida albicans morphogenesis we obtained a GFP fusion to determine its cellular localization. During growth in yeast form Phr1p-GFP was predominantly detected at discrete sites of the plasma membrane. Moreover Phr1p-GFP was recovered in the Detergent Resistant Membranes indicating its association with the lipid rafts as already described for wild type Phr1p.
Next we examined the localization of Phr1p during hyphal growth. Phr1p-GFP was detected at 30 min after a shift of stationary phase cells to condition of induction of hyphal growth. At this time point the fluorescence of Phr1-GFP was very bright and restricted at the tip of the germ tubes. Phr1p remained highly polarized at the apex of the hyphae as the hyphae grew. The protein also gradually distributed along the lateral cell wall as the hyphae elongated. A study of colocalization with chitin indicated that Phr1p is more abundant at the apex where chitin is less abundant. The results suggest that Phr1p is required at the site of maximal growth probably for the incorporation of new beta(1,3)-glucan in the expanding cell wall but is also retained along the hyphae where the cell wall becomes more cross-linked and resistant. Phr1p also showed an additional localization: it was detected along the septum region in vegetative growing cells and as a thin line in the septa of the hyphae indicating a potential role of the protein also at these sites.
Acknowledgement: this work was supported by RTN project Cantrain N. 51248 to LP
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
A genome-wide transcriptional analysis of the response to hyphal wall stress in Candida albicans
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Molecular approaches to identify novel targets for future development of antifungal agents
No full textDispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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