1,721,086 research outputs found

    Induction Kinetics of Photosystem I-Activated P700 Oxidation in Plant Leaves and Their Dependence on Pre-Energization

    No full text
    Absorbance changes ¿A 810 were measured in pea (Pisum sativum L., cv. Premium) leaves to track redox transients of chlorophyll P700 during and after irradiation with far red (FR) light under various preillumination conditions in the absence and presence of inhibitors and protonophorous uncoupler of photosynthetic electron transport. It was shown that cyclic electron transport (CET) in chloroplasts of pea leaves operates at its highest rate after preillumination of leaves with white light and is strongly suppressed after preillumination with FR light. The FR light-induced suppression was partly released during prolonged dark adaptation. Upon FR illumination of dark-adapted leaves, the induction of CET was observed, during which CET activity increased to the peak from the low level and then decreased gradually. The kinetics of P700 oxidation induced by FR light of various intensities in leaves preilluminated with white light were fit to empirical sigmoid curves containing two variables. In leaves treated with a protonophore FCCP, the amplitude of FR light-induced changes ¿A 810 was strongly suppressed, indicating that the rate of CET is controlled by the pH gradient across the thylakoid membran

    Analysis of initial chlorophyll fluorescence induction kinetics in chloroplasts in terms of rate constants of donor side quenching release and electron trapping in photosystem II

    No full text
    The fluorescence induction F(t) of dark-adapted chloroplasts has been studied in multi-turnover 1 s light flashes (MTFs). A theoretical expression for the initial fluorescence rise is derived from a set of rate equations that describes the sequence of transfer steps associated with the reduction of the primary quinone acceptor Q A and the release of photochemical fluorescence quenching of photosystem II (PSII). The initial F(t) rise in the hundreds of ¿s time range is shown to follow the theoretical function dictated by the rate constants of light excitation (k L) and release of donor side quenching (k si ). The bi-exponential function shows sigmoidicity when one of the two rate constants differs by less than one order of magnitude from the other. It is shown, in agreement with the theory, that the sigmoidicity of the fluorescence rise is variable with light intensity and mainly, if not exclusively, determined by the ratio between rate of light excitation and the rate constant of donor side quenching release

    Going Beyond Counting First Authors in Author Co-citation Analysis

    Get PDF
    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Licht en leven

    No full text
    Rede 29-11-79 Wageninge
    corecore