9,263,555 research outputs found
Indicadores estratégicos de gestión y de resultados para la Universidad Nacional de Villa María
No full textFil: Luque, Gustavo A.. Universidad Nacional de Villa María; Argentina.Fil: Riveros, Lucía Graciela. Universidad Nacional de Villa María; Argentina.Fil: Cantelli, Sandra Carina. Universidad Nacional de Villa María; Argentina.Fil: Conrero, Cristina Laura. Universidad Nacional de Villa María; Argentina.Fil: Vinanti, Mauricio, . Universidad Nacional de Villa María; Argentina.Fil: Jornet, María Guadalupe. Universidad Nacional de Villa María; Argentina.Fil: Jornet, Estefanía. Universidad Nacional de Villa María; Argentina.Fil: Obeide, Sergio F., . Universidad Nacional de Villa María; Argentina
Development of a novel approach to overcome biofilm formation by natural product derivatives
No full textWith the aim to prevent biofilm formation, and based on our previous studies, 1, 2 we explored new strategies
involving inorganic nanoparticles for surface covering. In detail, we investigated the functionalization of silica
nanoparticles with natural derivatives showing anti-biofilm properties, using linkers of different nature and
length. After their analytical characterization, the new derivatives were used to obtain glass coverslips by spin-coating technology for the biological testing (Figure 1). The promising results towards E. coli and P.
aeruginosa will be presented.
1 C. Cattò; S. Dell'Orto; F. Villa; S. Villa; A. Gelain; A. Vitali; V. Marzano; S. Baroni S; F. Forlani; F. Cappitelli. PLoS ONE 2015, 10(7), e0131519.
2 C. Cattò; G. Grazioso; S. Dell’Orto; A. Gelain; S. Villa, V. Marzano; A. Vitali; F. Villa; F. Cappitelli; F. Forlani. Biofouling 2017, 33, 235-25
Tiepolo a Villa Berg
No full textCatalogo edito in occasione dell’esposizione all’Ambasciata d’Italia a Villa Berg del dipinto "Enea, Anchise e Ascanio" (1773) di Giandomenico Tiepolo, avvenuta in data 20 luglio 2018 e curata da Giovanni Carlo Federico Villa. Il volume si compone di una serie di introduzioni istituzionali, saggi e una scheda catalografica. Contiene: " 1718-1797 Venezia e il dominio delle arti, in un secolo breve" / Giovanni Carlo Federico Villa (pp. 14-22); " Giandomenico Tiepolo: figlio, allievo, maestro" / Giovanni Carlo Federico Villa (pp. 23-29); Scheda / Giovanni Carlo Federico Villa (pp. 36-40)
New strategies to overcome biofilm formation by natural product derivatives
No full textBiofilm is a microbial community in which microorganisms are tightly bound to each other and
enclosed in a self-produced matrix (EPS). Bacterial biofilm is frequently present on moisty surfaces,
food, teeth, medical devices, water systems etc. In humans, biofilms can be damaging for the health,
causing and sustaining chronic infections due to their high antibiotic resistance, but also beneficial
(e.g., microbiome).
Among the different approaches to prevent biofilm formation, the new trend is to consider
antibiofilm agents acting at sublethal concentrations. In this way, the compounds inhibit biofilm
formation without killing the bacteria, thus avoiding resistance selection. Based on our previous
experience in the use of LDPE, covalently functionalized with natural product derivatives (paminosalicylic and p-aminocinnamic acids), for the development of antifouling materials for medical
devices1,2, we decided to improve and adapt our validated strategy to silica nanoparticles. Therefore,
we functionalized the starting material (Ludox HS-40) with the above-mentioned natural derivatives,
using linkers differing in nature and length, to obtain nanoparticles suitable for the coating
of various surfaces. The obtainment of the new systems, their characterization, the preparation of
the coating, and the biological evaluation of their antibiofilm activities will be presented.
References
1. Cattò C.; Dell'Orto S.; Villa F.; Villa S.; Gelain A.; Vitali A.; Marzano V.; Baroni S.; Forlani F.; Cappitelli F.; Plos One, 2015,10, 1-24.
2. Cattò C.; Grazioso G.; Dell’Orto S.; Gelain A.; Villa S., Marzano V.; Vitali A.; Villa F.; Cappitelli F.; Forlani F.; Biofouling, 2017, 33, 235-
Natural compounds targeting E. Coli WRBA as antibiofilm agents: Virtual screening, design and synthesis of analogs, biological evaluation
No full textResistance to antibiotics has become one of the main concerns of modern medicine. This
phenomenon is worsened by biofilm formation, which frequently leads to treatment failures.1 Hence,
anti-biofilm agents represent useful tools in the treatment of microbial infections, increasing the
effectiveness of antibiotics and preventing resistance mechanisms.
Different studies have demonstrated that the protein WrbA plays a key role in the formation of
biofilm in E. coli. Nonetheless, its specific function is still poorly understood, and only a few inhibitors
are reported in the literature.2
For this reason, our research group has worked towards the
identification of new WrbA inhibitors as antibiofilm agents.
Through a target-based virtual database screening, we identified natural products endowed with a
high affinity towards WrbA; the most promising compounds were purchased for biophysical and
biological evaluation.
Hence, we selected a natural scaffold as a starting point to create a new library of organic molecules
and perform a virtual screening. Finally, five compounds were selected to be synthesized and
subjected to biophysical evaluation.
Both the natural and synthetic compounds were tested on recombinant E. coli WrbA by MicroScale
Thermophoresis (MST), which allowed the determination of their dissociation constants (Kd). Among
the natural compounds, one of them displayed a Kd in the nanomolar range. This value was lower
than those measured for both caffeic and zosteric acid, which are known WrbA inhibitors.3 On the
other hand, the synthesized compounds bound WrbA with Kd values in the low micromolar range.
Based on our encouraging results, the inhibitors were assayed on E. coli and S. aureus to verify their
antibiofilm effect. The outcomes of our study will be presented.
References
1. Antimicrobial resistance https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance (accessed 2022-
05-10).
2. Rossi, F.; Cattò, C.; Mugnai, G.; Villa, F.; Forlani, F.; Pinto, A.; Nuzzo, D. Effects of the Quinone Oxidoreductase WrbA on Escherichia Coli Biofilm Formation and Oxidative Stress. Antioxidants 2021, 10 (6), 919.
3. Cattò, C.; Dell’Orto, S.; Villa, F.; Villa, S.; Gelain, A.; Vitali, A.; Marzano, V.; Baroni, S.; Forlani, F.; Cappitelli, F. Unravelling the Structural and Molecular Basis Responsible for the Anti-Biofilm Activity of Zosteric Acid. PLoS ONE 2015, 10 (7), E0131519
Autopercepción de riesgo ambiental y de salud en poblaciones expuestas a agrotóxicos
No full textFil: Bertone, Carola L., . Universidad Nacional de Villa María; Argentina.Fil: Delgado, María F., . Universidad Nacional de Villa María; Argentina.Fil: Miranda, Faustina D., . Universidad Nacional de Villa María; Argentina
Premessa
No full textpremessa al volume "Il postfotografico". Descrizione degli obiettivi del volume e delle sezioni in cui è suddiviso
Bitácora: construcción del territorio periurbano Villa María
No full textFil: Re, Virginia. Universidad Nacional Villa María; Argentina.Fil: Suarez, Valeria. Universidad Nacional Villa María; Argentina.Fil: Carriazo, Silvia R.. Universidad Nacional Villa María; Argentina.Fil: Moran, Ignacio, . Universidad Nacional de Villa María; Argentina.Fil: Furlan, M. Lucrecia. Universidad Nacional Villa María; Argentina.Fil: Mizdraje, Dafne. Universidad Nacional Villa María; Argentina.Fil: Re, Virginia. Universidad Nacional Villa María; Argentina.Fil: Caset, María Laura. Universidad Nacional Villa María; Argentina.Fil: Pierotti, Luciana. Universidad Nacional Villa María; Argentina.Fil: Riviera, Elisa. Universidad Nacional Villa María; Argentina.Fil: Castoldi, Leonardo, . Universidad Nacional de Villa María; Argentina.Fil: Becker, Analía, . Universidad Nacional de Villa María; Argentina.Fil: Guzmán L., Ana, . Universidad Nacional de Villa María; Argentina
Otro título: Música culta brasileña
No full textIntérpretes: Heitor Villa-Lobos. Orchestra Sinfonica BrasileiraCinta 1: Preludio n. 1. Min. 04.56: Palabras del propio Villa-Lobos sobre la música. Min. 23.10: Choros n. 1. Min. 27.12: Nhapopé. Min. 27.12: Guriatâ do coqueiro -- Cinta 2: Choros n. 5, Alma brasileira. Min. 04.37: Un canto que sai da senzala. Min. 07.25: Xango. Min. 08.48: Lenda do caboclo. Min. 12.41: Polichinel
Relevamiento del Sector de la Cerveza Artesanal Villa María-Villa Nueva
No full textEl presente informe sectorial surge desde la articulación entre la Cámara de Cerveceros de Villa María y la región (C.E.R.C.A.) y la Plataforma para el Desarrollo Productivo Territorial (PRODET), perteneciente al Instituto Académico Pedagógico de Ciencias Sociales de la Universidad Nacional de Villa María. La metodología utilizada en el presente estudio combina aspectos cuantitativos y cualitativos, a los fines de tener un acercamiento integral y complejo a la realidad sectorial. Para llevarlo a cabo se aplicó un cuestionario semi-estructurado a 10 cervecerías artesanales de las ciudades de Villa María y Villa Nueva, que forman parte de la Cámara de Cerveceros Villa María-Villa NuevaFil: Gasparrini, Guido. Universidad Nacional Villa María; Argentina.Fil: Peiretti, Josefina. Universidad Nacional Villa María; Argentina.Fil: Bergesio, Lilia. Universidad Nacional Villa María; Argentina
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