262,294 research outputs found
Data on heat tolerance for D latus from: Does plasticity in thermal tolerance trade off with inherent tolerance? The influence of setal tracheal gills on thermal tolerance and its plasticity in a group of European diving beetles
No full text<h3>Dataset on heat tolerance used in the study by Verberk et al., (2018).</h3><h3>Method description</h3><p>We assessed the impact of mode of respiration on heat tolerance under different oxygen conditions in one of the 15 species: <i>D. latus</i>, the most tolerant species in our comparison, using previously described methods (<a href="https://www.sciencedirect.com/science/article/pii/S0022191017302044#b0170">Verberk and Calosi, 2012</a>, <a href="https://www.sciencedirect.com/science/article/pii/S0022191017302044#b0185">Verberk and Bilton, 2015</a>). Briefly, individuals were placed in flow-through chambers, whose water supply could be heated. For one group of animals, we used chambers where the animals were completely submerged and had no access to air, while for a second group of animals chambers were used with a small head space holding a layer of air, meaning that these animals could obtain oxygen either from the air compartment by surfacing or from the water with oxygen diffusing directly into their tracheal system <i>via</i> the setae or oxygen diffusing into their subelytral air reservoir <i>via</i> their physical gill. Individuals were left to settle for 1 h at the equilibration temperature of 10 °C, after which the temperature was ramped up at 0.25 °C min−1. The CTmax was defined as the point at which animals lost coordinated swimming, hence losing their ability to escape from the conditions that will lead to their death (<a href="https://www.sciencedirect.com/science/article/pii/S0022191017302044#b0095">Lutterschmidt and Hutchison, 1997</a>). The heating rate, endpoint and starting temperature all therefore differed from the methodology described above, meaning that the critical thermal temperatures from both methods cannot be compared directly. CTmax was assessed under <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/normoxia">normoxia</a>, <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/hypoxemia">hypoxia</a> and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/hyperoxia">hyperoxia</a> conditions (5, 20, 60 kPa O2 respectively) and adults were assessed with and without access to air. Oxygen tension of both the water and the air in the headspace was altered to produce hypoxia and hyperoxia, as described by <a href="https://www.sciencedirect.com/science/article/pii/S0022191017302044#b0185">Verberk and Bilton (2015)</a>.</p>
Altered penetration of polyethylene glycols into uninvolved skin of atopic dermatitis patients.
No full textSupplement 1. Data and formulas used in the analyses on body size and richness.
No full textFile List
Verberk body size.xls
Verberk body size.csv
Verberk richness.xls
Verberk richness.csv
Description
[Please be aware that ESA cannot guarantee the forward migration (availability) of the Excel (.xls) files]
Verberk body size.xls gives all the data and formulae used to analyze the relationship between amphipod body size and clines in environmental conditions purportedly linked with oxygen (altitude, temperature, salinity).
Verberk body size.csv is a comma-delimited version of the body size worksheet, giving all the data and the calculated values for the oxygen supply index, but without the formulae.
Verberk richness.xls gives all the data and formulae used to analyze the relationship between species richness and clines in environmental conditions purportedly linked with oxygen (altitude, organic pollution, temperature).
Verberk richness.csv is a comma-delimited version of the richness worksheet, giving all the data and the calculated values for the oxygen supply index, but without the formulae.
Check sum values for:
Verberk body size.xls and .csv:
column 1 (Site descriptors): checksum is 136, no missing values
Verberk richness.xls:
column 1 (Site descriptors): checksum is 655, 3 missing values
Verberk richness.csv:
column 2 (Site descriptors): checksum is 655, 3 missing values
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Differences in percutaneous absorption in normal and atopic dermatitis skin in relation to molecular weigh.
No full textDraft version of paper data and code of manuscript: Body mass and cell size shape the tolerance of fishes to low oxygen in a temperature-dependent manner
No full textCite the repository as:
Verberk WCEP, Sandkler JF, van de Pol I, Urbina M, Wilson R, McKenzie DJ & Leiva FP (2022). Draft version of paper data and code of manuscript: Body mass and cell size shape the tolerance of fishes to low oxygen in a temperature-dependent manner. Zenodo. https://doi.org/10.5281/zenodo.6123770
Cell size may affect heat tolerance in Drosophila
No full textThe dataset contains raw data of cell size, cell number, wing size and survival time of Drosophila melanogaster used for the analyses and to generate figures of the Insect Science paper (https://doi.org/10.1111/1744-7917.12742) by Verspagen, Leiva, Janssen and Verberk. The article investigated how different larval densities and rearing temperatures affected cell size and heat tolerance in the fruit fly Drosophila melanogaster. A detailed description of the experiments is given in the Materials and Methods of the paper. Data are organized in two Excel files. The first one contains cell size, cell number and wing size for each of the treatments used and the second one contain survival time data for four stressful temperatures. A description of each of the column is also given
Draft version of paper data and code of manuscript: Body mass and cell size shape the tolerance of fishes to low oxygen in a temperature-dependent manner
No full textThis repository contains code and data needed to reproduce figures and tables of the manuscript:
Verberk WCEP, Sandkler JF, van del Pol I, Urbina M, Wilson R, McKenzie DJ & Leiva FP (2022). Body mass and cell size shape the tolerance of fishes to low oxygen in a temperature-dependent manner. Global Change Biology. https://doi.org/10.1111/gcb.16319
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Data on heat tolerance from: Field and laboratory studies reveal interacting effects of stream oxygenation and warming on aquatic ectotherms
No full text<p>Heat tolerance data on two widespread Eurasian mayflies, <i>Ephemera danica</i>, Müller 1764 and <i>Serratella ignita</i> (Poda 1761).</p><p>Methods thermal tolerance experiments</p><p>Mayfly nymphs for laboratory experiments were collected in spring (early May) from Torrington River, Devon, UK, ranging in fresh weight between 15 and 128 mg (<i>E. danica</i>) and between 2.0 and 11.6 mg (<i>S. ignita</i>). Nymphs were maintained in the laboratory at 10 ± 1 °C in a 12 L:12 D regime in aquaria containing artificial pond water, buffered and diluted to reflect the pH and conductivity of the field site. Before recording critical temperatures, all species were acclimated for at least 7 days to laboratory conditions.</p><p>To assess critical thermal maxima (<i>CT</i>max), we employed previously described methods (Verberk & Bilton, <a href="https://onlinelibrary.wiley.com/doi/full/10.1111/gcb.13240#gcb13240-bib-0051">2011</a>; Verberk & Calosi, <a href="https://onlinelibrary.wiley.com/doi/full/10.1111/gcb.13240#gcb13240-bib-0054">2012</a>). Individual nymphs (<i>n</i> = 18 for <i>E. danica</i> and <i>n</i> = 27 for <i>S. ignita</i>) were placed in flow-through chambers and water was supplied to these chambers from a header tank after having passed through a tubular counter-current heat exchanger. Water in the header tank was of the same composition as that used to maintain animals and was bubbled with a mixture of 20% oxygen and 80% nitrogen, obtained using a gas-mixing pump (Wösthoff, Bochum, Germany). Individuals were left resting for 1 h at the equilibration temperature of 10 °C, after which temperature in the experimental chambers was increased by 0.25 °C min−1, using a Grant R5 water bath with a GP200 pump unit (Grant Instrument Ltd, Cambridge, UK), connected to the heat exchanger. Temperatures were logged using a HH806AU digital thermometer (Omega Engineering Inc., Stamford, CT, USA). Different sized flow-through chambers were used for each species. <i>E. danica</i> was placed in larger chambers (70 × 70 × 30 mm) and provided with sand as burrowing substrate, which they readily used. <i>S. ignita</i> was placed in smaller cylindrical chambers (6 mm in diameter, 20 mm long) and their behaviour was observed under a magnifying glass. The amount of water passing through these flow-through chambers was matched to their size. For the larger chambers containing <i>E. danica</i>, water was supplied to five chambers (total volume of 0.735 l) at a flow rates of 0.031–0.033 l per second, resulting in a refresh rate of 22–24 s. For the smaller chambers with <i>S. ignita</i>, water was supplied to each chamber individually at 0.21–0.22 ml per second, resulting in a refresh rate of 10–11 s.</p><p><i>CT</i>max is defined as the point at which an animal loses its ability to escape from conditions that will lead to its death (Lutterschmidt & Hutchison, <a href="https://onlinelibrary.wiley.com/doi/full/10.1111/gcb.13240#gcb13240-bib-0027">1997</a>). During progressive warming, nymphs of <i>E. danica</i> first emerged from their burrowed position and began swimming (at about 6 °C below <i>CT</i>max). Loss of equilibrium occurred next as nymphs fell upon their backs, which was followed by the onset of spasms. After that, gill movement was no longer coordinated and faltered and this endpoint could be most reliably determined and is here taken as <i>CT</i>max. Similarly<i>, S. ignita</i> stopped ventilation and movement at <i>CT</i>max. Below <i>CT</i>max, larvae were inactive, until near the end of the trials, when they began to crawl, lose equilibrium and gill beating became intermittent shortly before stopping altogether at <i>CT</i>max.</p><p><i>CT</i>max was assessed at hypoxic (5 kPa), normoxic (20 kPa) and hyperoxic (60 kPa) conditions. Different levels of oxygenation were achieved by changing the oxygen–nitrogen gas mixture obtained using the gas-mixing pump (Wösthoff). The gas mixture was adjusted 10 min after placing the animals in the small flow-through chambers, to allow for gradual exposure to hypoxic and hyperoxic conditions during the 1 h resting period. To prevent equilibration with the atmosphere, the header tank was sealed using an 18 mm thick expanded polystyrene sheeting and other openings were closed off with plastic material. During the 1 h resting period, oxygen levels in the outflow water from the chambers were measured approximately every 15 min, to verify that the oxygen levels had stabilized to hypoxic, normoxic and hyperoxic conditions at the onset of warming. Because some equilibration with the atmosphere could not be prevented, nominal output values from the gas mixer were slightly more extreme (3 kPa for hypoxia and 65 kPa for hyperoxia) in order to achieve the desired oxygen conditions in the test chambers.</p>
Dataset belonging to the manuscript entitled: It's about time: Linkages between heat tolerance, thermal acclimation and metabolic rate at different temporal scales in the freshwater amphipod Gammarus fossarum Koch, 1836
No full textThis dataset contains raw data of respiration, survival and thermal sensitivity of respiration and survival of cold and warm acclimated amphipods used for analyses and figures in the research described in the Journal of Thermal Biology: It's about time: Linkages between heat tolerance, thermal acclimation and metabolic rate at different temporal scales in the freshwater amphipod Gammarus fossarum Koch, 1836. (2018) by Semsar-kazerouni and Verberk. In this paper, we examined the effect of temperature acclimation on heat tolerance and metabolism in the aquatic ectotherm Gammarus fossarum. Details about the experimental procedures are given in the Materials and Methods section of the paper. We have also included the R scripts for data analyses in this dataset. A brief description of the data files and column descriptions can be found below
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