169,777 research outputs found

    Purification and characterization of 3,4-dihydroxyphenylalanine decarboxyase from pig kidney

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    A procedure for 3,4-dihydroxyphenylalanine decarboxylase from pig kkdney purification is described in detail. The preparation has no detectable impurity on electrophoresis and on ultracentrifugation and authors. However two significant differences are observed: a different stimulation of activity by added pyridoxal 5'-phosphate and a nearly complete decarboxylation of L-3,4-dihydroxyphenylalanine in absence of added coenzyme. Absorption, fluorescence and circular dichroism properties of the coenzyme-apoenzyme interaction are also described. The results are consistent with the existence of at least four coenzyme-apoenzyme complexes, three of them active

    PROCESS FOR TREATING CHAR FROM RECYCLED PLASTICS

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    A process for recovering the carbon contained in a char (solid carbonaceous residue) from a pyrolysis of mixed plastic waste or secondary raw material obtained from the recycling and/or treatment of plastic wastes is described, said process comprising the following steps: (A) heating said char in a gasification reactor and in the presence of carbon dioxide to reach a given temperature comprised in a range lower than 950°C and higher than 600°C, preferably lower than or equal to 850°C; (B) continue heating said char in the aforementioned temperature range, preferably in the range from 750 °C to 850 °C, in the presence of CO2, for a given residence time of said char in said reactor producing an outflow gas stream (effluent) comprising CO in addition to unreacted CO2, and possibly H2, (C) sending at least a part of the gas stream (effluent) leaving the reactor comprising CO, unreacted CO2, and optionally H2, to at least one subsequent operating unit selected from a unit for separating CO from CO2, a direct use unit of said outflow stream or to a storage unit

    Calcium- and pH-linked oligomerization of sorcin causing translocation from cytosol to membranes

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    Sorcin, a cytosolic calcium-binding protein containing a pair of EF-hand motifs, undergoes a Ca2+-dependent translocation to the cell membrane, The underlying conformational change is similar at pH 6.0 and 7.5 and consists in an increase in overall hydrophobicity that involves the aromatic residues and in particular the two tryptophan residues which become less exposed to solvent, The concomitant association from dimers to tetramers indicates that the tryptophan residues, which are located between the EF-hand sites, become buried at the dimer-dimer interface, Ca2+-bound sorcin displays a striking difference in solubility as a function of pH that has been ascribed to the formation of calcium-stabilized aggregates. (C) 1997 Federation of European Biochemical Societies

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Neutron scattering and modeling of dipole-field-induced spin disorder in Nanoperm

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    peer reviewedWe present magnetic-field-dependent small-angle neutron scattering data for the ferromagnetic nanocomposite Nanoperm (Fe89Zr7B3Cu1). The spin-misalignment scattering in the approach-to-saturation regime unexpectedly reveals pronounced lobes of high intensity at angles ±30−40° relative to the magnetic-field axis. Based on numerical calculations, the four-fold angular symmetry of the scattering pattern can be explained in terms of local spin misalignment, which originates from dipolar stray fields due to the mismatch of the saturation-magnetization values between the bcc Fe particles and the amorphous magnetic matrix
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