12,579 research outputs found

    Differential stability of 2'F-ANA*RNA and ANA*RNA hybrid duplexes: roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility

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    14 pags., 7 figs., 3 tabs.Hybrids of RNA with arabinonucleic acids 2′F-ANA and ANA have very similar structures but strikingly different thermal stabilities. We now present a thorough study combining NMR and other biophysical methods together with state-of-the-art theoretical calculations on a fully modified 10-mer hybrid duplex. Comparison between the solution structure of 2′F-ANA•RNA and ANA•RNA hybrids indicates that the increased binding affinity of 2′F-ANA is related to several subtle differences, most importantly a favorable pseudohydrogen bond (2′F-purine H8) which contrasts with unfavorable 2′-OH-nucleobase steric interactions in the case of ANA. While both 2′F-ANA and ANA strands maintained conformations in the southern/eastern sugar pucker range, the 2′F-ANA strand's structure was more compatible with the A-like structure of a hybrid duplex. No dramatic differences are found in terms of relative hydration for the two hybrids, but the ANA•RNA duplex showed lower uptake of counterions than its 2′F-ANA•RNA counterpart. Finally, while the two hybrid duplexes are of similar rigidities, 2′F-ANA single strands may be more suitably preorganized for duplex formation. Thus the dramatically increased stability of 2′F-ANA•RNA and ANA•RNA duplexes is caused by differencesin at least four areas, of which structure and pseudohydrogen bonding are the most important. © The Author(s) 2010. Published by Oxford University Press.Spanish Ministerio de Ciencia e Innovacion (grants CTQ2007-68014-C02-02 to CG and BIO2009-10964 to MO); Fundacion Marcelino Botin (grant to MO); Canadian Institutes for Health Research (grant to M.J.D.); Natural Sciences and Engineering Research Council of Canada (postgraduate scholarship to J.K.W.). Funding for open access charge: Canadian Institutes for Health Research

    Representações da sexualidade na obra de Ana Saldanha

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    Mestrado em Línguas, Literaturas e CulturasA presente dissertação analisa as diferentes representações da sexualidade na obra literária para jovens de Ana Saldanha. A leitura analítica incide sobre as narrativas da autora que abordam a temática da sexualidade juvenil e as suas problemáticas, com o objetivo de estudar a forma como as mesmas contribuem para a construção da identidade pessoal e sexual do potencial recetor juvenil. Desta forma, pretende-se detetar os conflitos psicológicos dos protagonistas, os seus valores e problemas, assim como os novos valores, problemas e preocupações sociais que emergem das narrativas; compreender de que forma a autora exprime a sua capacidade em observar o quotidiano e as relações humanas; identificar os pilares em que se apoia o universo social em estudo (família, escola, grupo de amigos); enquadrar as personagens numa sociedade pós-industrial e de informação que exige ao adolescente uma superação de desafios e de novos problemas.This dissertation analyzes the different representations of sexuality in Ana Saldanha’s literary work for youth. The analytical reading focuses on the author's narratives that address the issue of teenage sexuality and its problems, aiming to study their contribution in the construction of personal and sexual identity of the potential juvenile receiver. Thus, it is intended to detect the psychological conflicts of the protagonists, their values and problems, as well as the new values, social concerns and problems that emerge from the narratives; understand how the author expresses her ability to observe daily life and human relations; identify the pillars on which relies the social universe under study (family, school, friends); framing the characters in a post-industrial and information society which requires the teenager to relentlessly surmount challenges and new problems

    PRIKAZ ZBORNIKA „ŽIVOT MORA BITI DJELO DUHA“ U ČAST PROFESORICI DUBRAVKI SESAR

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    Zrinka Kovačević i Ivana Vidović Bolt (ur.), 2017, Život mora biti djelo duha, Zbornik posvećen prof. dr. sc. Dubravki Sesar; Zagreb, Disput, 386. str

    Viajantes ex-cêntricas nas histórias de Ana Miranda

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    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-Graduação em Literatura.No conjunto das narrativas ficcionais da escritora brasileira Ana Miranda, a temática da viagem # considerada uma das mais férteis da literatura ocidental de todos os tempos # ocupa um espaço de centralidade, podendo até mesmo ser vista como o mais importante eixo de estruturação de suas obras. O estudo realizado pela presente tese tem por objetivo analisar o tratamento dado ao tema da viagem nos romances Desmundo, O retrato do rei, Dias & Dias e Amrik, evidenciando que por intermédio das narradora-viajantes # Oribela, Mariana, Feliciana e Amina # a autora promove um diálogo entre diferentes culturas, gêneros, etnias e gerações, ao mesmo tempo em que estabelece um profícuo diálogo com o passado em sua invariante problematização concernente aos limites e cruzamentos entre o discurso ficcional e os discursos narrativos extraliterários que o cercam, sobretudo o histórico e o biográfico. In the whole of Brazilian writer Ana Miranda#s fictional narrative, the travel thematic # considered one of the most fertile themes of the western literature of all times # occupies a central space, and can even been seen as the most important structuring axis of Miranda#s works. The aim of the present thesis is to analyze the treatment given to the travel subject in the novels Desmundo, O retrato do rei, Dias & Dias and Amrik, emphasizing that, through the traveling narrators, Oribela, Mariana, Feliciana and Amina, the author promotes a connection between different cultures, genders, ethnics and generations, at the same time that establishes a profitable dialogue with the past in her invariant problematization concerning the limits and crossings between the fictional and the extra-literary narrative discourses that surrounds it, mainly the historical and the biographic ones

    Séquence 4, Ana et la télévision, raconté par Ana

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    Cette séquence fait partie du corpus vidéo de Langue des Signes Emergentes utilisé lors de la thèse d'Ivani Fusellier-Souza : la locutrice Ana raconte qu'elle aime regarder la télé, bien que ne peut l'écouter. Alors que sa mère ne la regarde pas.00:00:00>00:00:03 La sœur demande à Ana si elle aime bien regarder la télévision. 00:00:04>00:00:07 Ana dit qu'ils ont une télé là-bas. 00:00:07>00:00:15 La sœur demande à nouveau à Ana si elle aime ou non la télé. 00:00:15>00:00:19 La mère dit qu'il faut faire attention, car si on allume la télé, Dieu nous punira. 00:00:19>00:00:48 Ana et sa sœur discute sur le fait qu'Ana ne peut pas écouter la télé et peut seulement la regarder, alors que ses sœurs peuvent l'écouter. 00:00:48>00:01:09 La sœur lui demande ce qu'elle pense des filles à la télé en mini-jupe avec des débardeurs et du rouge à lèvres. 00:01:09>00:01:35 Ana dit qu'elle n'aime pas comme sa sœur les filles qui s'habillent comme cela. 00:01:35>00:01:39 La mère dit que de toute façon, Dieu les punira. 00:01:40>00:01:53 Ana explique qu'elle regarde la télé avec ses sœurs mais que sa mère ne la regarde pas

    Gender stereotypes in Bulgarian and Croatian animalistic phraseology

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    Ovaj diplomski rad bavi se rodnim stereotipima u bugarskoj i hrvatskoj animalističkoj frazeologiji, tj. bavi se frazemima koji se u upotrebi potpuno ili barem djelomično odnose na referente muškog ili ženskog spola, a za sastavnicu imaju naziv za životinju (Hrnjak, 2017). U prvom, teorijskom dijelu rada definiraju se pojmovi frazeologija i frazem i opisuje se razvoj discipline, obilježja frazema i različiti aspekti u proučavanju frazema. U poglavlju o animalističkoj frazeologiji iznose se temeljne postavke kulturne zoologije i objašnjavaju se pojmovi zoomorfizma i antropomorfizma (Barčot, 2017). Osim toga, objašnjava se uloga spola i roda u jeziku općenito te u frazeologiji. U istraživačkom dijelu diplomskog rada provodi se kontrastivna analiza frazeološkog korpusa, prikupljenog iz frazeoloških i općih rječnika, relevantnih radova i mrežnih korpusa. Cilj istraživanja ovoga diplomskoga rada je utvrditi u kojoj se mjeri podudaraju bugarski i hrvatski frazemi, koje su životinje sastavnica frazema i koja je njihova motivacija. Rezultat je kreiranje slike muškarca i žene kroz prizmu bugarske i hrvatske animalističke frazeologije.This master’s thesis deals with gender stereotypes in Bulgarian and Croatian animalistic phraseology, i.e., it deals with idioms that in use fully or at least partially refer to male or female referents and for their component have an animal name (Hrnjak, 2017). In the first, theoretical part of the thesis, the terms phraseology and idiom are defined and the development of the discipline, characteristics of idioms and various aspects in the study of idioms are described. In the chapter of animalistic phraseology the basic postulations of cultural zoology and the concepts of zoomorphism and anthropomorphism are presented (Barčot, 2017). In addition to this, the role of sex and gender in language in general and in phraseology are explained. In the research part of the thesis is conducted contrastive analysis of the phraseological corpus, collected from phraseological and general dictionaries, relevant papers and online corpora. The aim of the research of this master’s thesis is to determine the extent to which Bulgarian and Croatian phrases coincide, which animals are components of phrases and what is their motivation. The result is the creation of an image of a man and a woman through the prism of Bulgarian and Croatian phraseology

    Poredbene konstrukcije i njihova rodna obilježenost u hrvatskom, slovenskom i srpskom jeziku: kontrastivna korpusna analiza

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    Tema diplomskog rada je analiza i usporedba poredbenih konstrukcija s varijabilnom imenskom sastavnicom u trima južnoslavenskim jezicima – hrvatskom, slovenskom i srpskom [lijep/a kao/ko C] [ružan/na kao/ko C]; [pametan/na kao/ko C] [glup/a kao/ko C]. Istraživanje je provedeno na temelju građe dobivene mrežnim korpusima hrvatskog, slovenskog i srpskog jezika hrWaC, slWaC i srWaC te su prikupljeni dodatni podaci za hrvatski jezik na temelju ankete. Poredbene konstrukcije su odabrane u oblicima za oba roda kako bi se što bolje prikazale razlike i sličnosti između oblika za muški i ženski rod odnosno kako bi se ispitala i prikazala rodna obilježenost konstrukcija. Metode korištene u svrhu analiziranja podataka su (1) leksikografska analiza koja obuhvaća podatke dobivene iz rječnika, (2) korpusna analiza, (3) analiza rodne obilježenosti poredbenih konstrukcija i (4) anketno ispitivanje izvornih govornika hrvatskog jezika. Podudarna korpusna analiza uključuje tri jezika, dok je za hrvatski jezik dodatno provedena i anketa.The topic of the thesis is the analysis and comparison of idiomatic constructions with a variable noun component in three South Slavic languages – Croatian, Slovenian and Serbian [lijep/a kao/ko C] [ružan/na kao/ko C]; [pametan/na kao/ko C] [glup/a kao/ko C]. The research was conducted on the basis of material obtained from online corpora of the Croatian, Slovenian and Serbian languages hrWaC, slWaC and srWaC, and additional data for the Croatian language was collected based on a survey. Phrasemic constructions were selected in the forms for both genders in order to better show the differences and similarities between the forms for the male and female genders, that is, in order to examine and show the gendered nature of the constructions. The methods used to analyze the data are (1) lexicographic analysis, which includes data obtained from dictionaries, (2) corpus analysis, (3) analysis of the gender nature of comparative constructions and (4) a survey of native speakers of the Croatian language. The corresponding corpus analysis includes three languages, while a survey was additionally conducted for the Croatian language

    Poredbene konstrukcije i njihova rodna obilježenost u hrvatskom, slovenskom i srpskom jeziku: kontrastivna korpusna analiza

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    Tema diplomskog rada je analiza i usporedba poredbenih konstrukcija s varijabilnom imenskom sastavnicom u trima južnoslavenskim jezicima – hrvatskom, slovenskom i srpskom [lijep/a kao/ko C] [ružan/na kao/ko C]; [pametan/na kao/ko C] [glup/a kao/ko C]. Istraživanje je provedeno na temelju građe dobivene mrežnim korpusima hrvatskog, slovenskog i srpskog jezika hrWaC, slWaC i srWaC te su prikupljeni dodatni podaci za hrvatski jezik na temelju ankete. Poredbene konstrukcije su odabrane u oblicima za oba roda kako bi se što bolje prikazale razlike i sličnosti između oblika za muški i ženski rod odnosno kako bi se ispitala i prikazala rodna obilježenost konstrukcija. Metode korištene u svrhu analiziranja podataka su (1) leksikografska analiza koja obuhvaća podatke dobivene iz rječnika, (2) korpusna analiza, (3) analiza rodne obilježenosti poredbenih konstrukcija i (4) anketno ispitivanje izvornih govornika hrvatskog jezika. Podudarna korpusna analiza uključuje tri jezika, dok je za hrvatski jezik dodatno provedena i anketa.The topic of the thesis is the analysis and comparison of idiomatic constructions with a variable noun component in three South Slavic languages – Croatian, Slovenian and Serbian [lijep/a kao/ko C] [ružan/na kao/ko C]; [pametan/na kao/ko C] [glup/a kao/ko C]. The research was conducted on the basis of material obtained from online corpora of the Croatian, Slovenian and Serbian languages hrWaC, slWaC and srWaC, and additional data for the Croatian language was collected based on a survey. Phrasemic constructions were selected in the forms for both genders in order to better show the differences and similarities between the forms for the male and female genders, that is, in order to examine and show the gendered nature of the constructions. The methods used to analyze the data are (1) lexicographic analysis, which includes data obtained from dictionaries, (2) corpus analysis, (3) analysis of the gender nature of comparative constructions and (4) a survey of native speakers of the Croatian language. The corresponding corpus analysis includes three languages, while a survey was additionally conducted for the Croatian language

    Gender stereotypes in Bulgarian and Croatian idioms with a zoonymic component

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    Ovaj rad se bavi istraživanjem rodnih stereotipa u bugarskim i hrvatskim frazemima koji za sastavnicu imaju domaću životinju, a odnose se isključivo na referenta muškog ili ženskog spola. Na početku rada dan je kratak pregleda razvoja frazeologije kao znanstvene discipline u Hrvatskoj i Bugarskoj, zatim se govori o obilježjima frazema u obje frazeološke tradicije i objašnjavaju se glavne postavke rodne i zoonimske frazeologije. Metoda koja je korištena u radu je kontrastivna analiza, uspoređeni su frazemi prema podacima iz rječnika, ali je njihova upotreba provjerena pretragom korpusa hrvatskog i bugarskog jezika Uočeno je snažno poklapanje rodnih stereotipa u oba jezika u velikom broju domena. Muškarce se smatra snažnima, svojeglavima, oholima, ali i stoičnima. Uz žene se vezuje manjak intelektualnih sposobnosti, smatraju se brbljavima te se velika pozornost obraća na njihov fizički izgled.This thesis deals with gender-specific stereotypes in Bulgarian and Croatian idioms that have a domestic animal as a component and that refer to only one gender. At the very beginning, a short overview of the development of phraseology as a science in Bulgaria and Croatia is provided, then the characteristics of an idiom in both Bulgarian and Croatian phraseology are described and the main theoretical framework of gender and zoonymic phraseology is defined. The methodology used in this thesis is lexis-based contrastive analysis applied to a collection of Bulgarian and Croatian idioms. To note the gender-specific use of an idiom, a search was performed in the corpora of Bulgarian and Croatian languages. A significant overlap of gender stereotypes in both languages was observed in substantial language domains. Men are considered to be strong, stubborn, arrogant, but also stoic. On the other hand, women are considered to be intellectually deficient, chatty and considerable attention is given to their physical appearance
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