1,721,039 research outputs found
KLIPPEL-FEIL SYNDROME IN AN ANCIENT SARDINIAN POPULATION (16TH CENTURY AD). A PALEOPATHOLOGICAL STUDY OF FOUR CASES FROM THE SAN MICHELE CEMETERY IN ALGHERO
No full textTransient Switch to myeloid lineage in Acute Lymphoblastic Leukemia in Induction: role of CD371 expression and implication for Minimal Residual Disease detection
Full text linkBackground: ImmunoPhenotyping (IP) by multi-colour flow cytometry (FCM) is a cornerstone of pediatric B-lineage acute lymphoblastic leukemia (BCP-ALL) diagnosis and is gaining ever greater prognostic role in minimal residual disease (MRD) monitoring. It allows also to detect lineage switch (SW), defined as any variation of blast IP during therapy. SWs to myeloid lineage were described in ALLs with KMT2A rearrangements and BCP-ALL expressing antigen CD2. We observed a transient SW to myelomocitic lineage during the first phase of AIEOP-BFM ALL 2009 protocol (Induction IA-steroid phase) in a subset of BCP-ALL. This behaviour was seen in association with the aberrant expression of myeloid antigen CD371 at diagnosis. We retrospectively compared CD371-positive (CD371+) to CD371-negative (CD371-) BCP-ALLs.
Aims of our study: to verify the association between CD371 expression at diagnosis and transient SW during Induction phase; to define SW blasts immunophenotypic changes and redefine a standardized approach to accurate FCM-MRD analysis on Day (D) 15.
Materials and Methods: Inclusion criteria: new diagnosis of BCP-ALL, except B-IV-ALL according to EGIL classification; treatment regimen: AIEOP BFM ALL 2009 protocol; enrolment period: 01/06/2014 – 31/01/2017. Eight-hundred-twenty-three paediatric patients (pts) were included in our study (age: 1-17 years; male/female 446/377 pts). Peripheral and bone marrow samples were centralized from all Italian AIEOP Centres to the Laboratory of Diagnosis and Research of Pediatric Hematology Oncology, University of Padua. Samples were processed and analysed according to standardized operating protocols designed by the AIEOP-BFM Flow Network. Nine combinations of 8 monoclonal antibodies were used for IP at diagnosis, 2 for FCM-MRD from June 2014 to May 2016; subsequently dry 10 colours pre-formulated DuraClone 10 Conj Custom Mix, Per Test, 2500 Test per Yr (Beckman Coulter) was adopted. BD FACS Canto II (Becton Dickinson) and Navios (Beckman Coulter) cytometers were used for samples acquisition and analysis.
Results: CD371+ was detected in 75/823 pts (9.1%) at diagnosis. CD371+ was associated with older age (>9 years: 34/75 vs 130/748 pts, p<.001); DNA index=1.0 (65/75 vs 437/748 pts, p<.001); immature immunophenotype according to EGIL classification (BI-ALL 10/75 vs 12/748 pts; BII-ALL 65/75 vs 555/748 pts, BIII-ALL 0/75 vs 181/478 pts, p<.001); CD2 positivity (38/75 vs 3/748 pts, p<.001); other 3 myeloid antigen positivity (42/75 vs 240/748 pts, p<.001); worse response to Induction therapy (high risk group: 27/75 vs 147/748 pts, p<.001). Samples of 72/75 pts (96%) were available for FCM-MRD analysis. SW was defined as the appearance of a «monocytoid» population characterized by the following immunophenotype: CD34 strong; CD58 strong; CD19 dim positive/negative; increased CD45 expression and Side Scatter characteristic. SW was observed in 50/72 CD371+ pts vs 4/748 CD371- pts (sensitivity 0.93, 95%IC ±0.06; specificity 0.98, 95%IC ±0.005; PPV 0.82; NPV 0.99; accuracy 0.98). CD371+ pts: SW was detected in 26/42 evaluated pts (61.9%) on D8 and 50/72 pts (69.4%) on D15. No more SW was detectable on D33 and D78, despite chemotherapy regimen according to AIEOP-BFM ALL 2009 protocol had been carried on.
Conclusions: CD371 is an accurate marker for the detection of transient SW in BCP-ALL. CD371+ is associated with worse response to Induction therapy. It should suggest peculiar attention in FCM-MRD analysis on Day 15 in these pts
Moving forward in target antigen discovery for immunotherapy in acute myeloid leukemia
Full text link: Not available
17th-century sculptural representation of leprosy in Perugia’s Cathedral
No full textRepresentation of leprosy in Perugia’s Cathedra
The Covid-19 pandemic as a communication responsibility and opportunity for paleopathology
No full textNarrating the famous Plague of Athens (430-426 BC) in its destructive manifestation, Thucydides was the first Western author to offer in his History of the Peloponnesian War (2.51) a compelling description of immunity acquired against a highly lethal infectious agent:[...] more often the sick and the dying were tended by the pitying care of those who had recovered, because they knew the course of the disease and were themselves free from apprehension. For no one was ever attacked a second time, or not with a fatal result [δὶς γὰρ τὸν αὐτόν, ὥστε καὶ κτείνειν, οὐκ ἐπελάμβανεν.] » (1
A depiction of poliomyelitis in a 17th -century Piedmontese fresco?
No full textIntroduction: A potential representation of poliomyelitis is investigated in an Italian artwork. Materials and methods: A 17th century Piedmontese fresco is analyzed by combining historico-medical, palaeopathological and clinical approaches. Alternative diagnoses are considered. Results, discussion and conclusions: The man appearing in the fresco holding a crutch is characterized by an atrophic left leg reminiscent of poliomyelitic atrophic. Other congenital anomalies or cerebrovascular causes appear less likely. A reflection on the difficulty of retrospectively diagnosis poliomyelitis is offered
Excavating and communicating human remains: the experience of the ArcheoTroina Project (Enna, Sicily)
No full textThis brief note aims to present to the readership of the PPA the interaction between the disciplines of archaeology and paleopathology by the summer-time activities of the “Archeotroina Project” that, since 2017 has been excavating the medieval site of Troina (Enna, Sicily) by combining field work with theoretical and practical lectures, research, and an involvement of the general public, and local institutions
Picasso’s Science and Charity and the evolution of the medical art
No full textThe present article reflects on the evolution of clinical medicine throughout time by commenting on Picasso's painting Science and Charity (1897) through a biomedical lens. The two souls of medicine, namely the cold scientific one and the compassionate one, are examined in their dichotomy and their relationship with today's concepts of cure and well-being
Correction to: Did Antonio Vallisneri (1661–1730) really describe frontal sinus osteoma? Unexpected insights for paleo-neuroparasitology (Neurological Sciences, (2018), 39, 7, (1275-1277), 10.1007/s10072-018-3312-7)
No full textThe presence of Homo in Sicily: evidence, hypotheses and uncorroborated ideas. An archaeo-anthropological perspective
Full text linkThis article summarises the main findings and data on the ancient peopling of the Mediterranean
island of Sicily through an archaeo-anthropological perspective. The hypothesis surrounding the presence
of the Lower Palaeolithic in Sicily with more ancestral species of Homo is also extensively reviewed and it
is explained why there are not sufficient elements to maintain it. Finally, future multidisciplinary proposals
are made to fill the gap on Sicilian cave archaeology
- …
