146 research outputs found
Flow cytometry data summary of clinical research study AFFECT
No full textSummary of all flow cytometry scatter plots and gates applied in the
clinical research study "Ticagrelor attenuates the increase of
extracellular vesicles concentrations in plasma after acute myocardial
infarction compared to clopidogrel" by Aleksandra Gasecka, Rienk Nieuwland, Monika
Budnik, Françoise Dignat-George,
Ceren Eyileten, Paul Harrison, Romaric Lacroix, Aurelie Leroyer, Grzegorz Opolski, Kinga Pluta, Edwin van der Pol, Marek Postuła, Pia R.M. Siljander, Jolanta M. Siller-Matula, Krzysztof Filipiak, which was accepted on November 27th
2019 for publication in the Journal of Thrombosis and Haemostasis. The Minimum Information
on Flow Cytometry experiments on Extracellular Vesicles (MIFlowCyt-EV) report
can be found as supplementary information of the manuscript. Raw data and data with
standard units (40 GB) will become available via SURFfilesender upon request ([email protected]).</p
Detection of extracellular vesicles: size does matter
No full textCells release small sacks filled with fluid, which are called "extracellular vesicles". The diameter of extracellular vesicles (EV) typically ranges from 30 nm to 1 µm. Because cells release EV into their environment, our body fluids contain numerous EV. Cells release EV to remove waste and to transport cargo to other cells. Since the size, concentration, cellular origin, and composition of EV in body fluids change during disease, EV have promising clinical applications, such as diagnosis of cancer. However, clinical applications of EV are not realized yet, because currently used detection techniques lack the sensitivity to detect the majority of EV. The aim of this thesis is to improve the detection of EV by (1) obtaining insights into physical properties of EV, and (2) gaining a profound understanding of techniques to detect EV. This thesis explains why the reported concentrations of EV in human blood plasma differ 100,000,000-fold. In addition, this thesis shows that flow cytometry detects large vesicles and swarms of smaller vesicles, which both are counted as a single event signal. The relationship between light scattering and the diameter of EV is modelled using Mie theory to demonstrate that a currently widely applied standardization procedure for EV detection selects EV and cells with a diameter of 800-2,400 nm instead of the envisioned 500-900 nm. Furthermore, a method based on nanoparticle tracking analysis is developed to determine the size and refractive index of single EV and other nanoparticles
Estudio de las oscilaciones electromagneticas en los circuitos RLC y análisis teórico del circuito de Van Der Pol.
No full textEn este trabajo se analizaron los distintos tipos de oscilaciones electromagnéticas en los circuitos RLC serie y paralelo. Estos tipos de circuitos se pueden describir con una ecuación diferencial de segundo orden cuya solución general incluye varios modos de oscilación y las respuestas de estos circuitos varían según sus componentes R, L y C.
Se tomaron varios valores de L, C y R experimentalmente, se realizaron diagramas de Bode para mostrar el desfasaje entre tensión y corriente en la bobina y en el capacitor. Se realizó un análisis teórico y computacional del circuito de Van der Pol el cual constituye una opción electrónica de mucho interés pues sus oscilaciones se estabilizan cuando se logra introducir un impulso no lineal. Investigaciones futuras en este campo deben proponerse el logro de circuitos experimentales correspondientes a este oscilador
Production Functions for Climate Policy Modeling: An Empirical Analysis
No full textQuantitative models for climate policy modeling differ in the production structure used and in the sizes of the elasticities of substitution. The empirical foundation for both is generally lacking. This paper estimates the parameters of two-level CES production functions with capital, labour and energy as inputs, and is the first to systematically compare all nesting structures. Using industry-level data from 12 OECD countries, we find that the nesting structure where capital and labour are combined first, fits the data best, but for most countries and industries we cannot reject that all three inputs can be put into one single nest. These two nesting structures are used by most climate models. However, while several climate policy models use a Cobb-Douglas function for (part of the) production function, we reject elasticities equal to one, in favour of considerably smaller values. Finally we find evidence for factor-specific technological change. With lower elasticities and with factor-specific technological change, some climate policy models may find a bigger effect of endogenous technological change on mitigating the costs of climate policy.Climate Policy, Input Substitution, Technological Change
MIFlowCyt-EV_EDTA_vs_citrate_def.pdf
No full textMIFlowCyt-EV file containing a description of the flow cytometry experiments conducted in the manuscript "EDTA stabilizes the concentration of platelet-derived extracellular vesicles during and after blood collection
Flow_cytometry_dataset_EDTA_vs_citrate.7z
No full textFlow cytometry dataset corresponding to manuscript "EDTA stabilizes the concentration of platelet-derived extracellular vesicles during and after blood collection"
From platelet dust to gold dust: physiological importance and detection of platelet microvesicles
No full textMisinterpretation of solid sphere equivalent refractive index measurements and smallest detectable diameters of extracellular vesicles by flow cytometry
No full textProduction of L(+)-lactic acid from acid pretreated sugarcane bagasse using Bacillus coagulans DSM2314 in a simultaneous saccharification and fermentation strategy
No full textBackground: Sugars derived from lignocellulose-rich sugarcane bagasse can be used as feedstock for production of L(+)-lactic acid, a precursor for renewable bioplastics. In our research, acid-pretreated bagasse was hydrolysed with the enzyme cocktail GC220 and fermented by the moderate thermophilic bacterium Bacillus coagulans DSM2314. Saccharification and fermentation were performed simultaneously (SSF), adding acid-pretreated bagasse either in one batch or in two stages. SSF was performed at low enzyme dosages of 10.5-15.8 FPU/g DW bagasse. Results: The first batch SSF resulted in an average productivity of 0.78 g/l/h, which is not sufficient to compete with lactic acid production processes using high-grade sugars. Addition of 1 g/l furfural to precultures can increase B. coagulans resistance towards by-products present in pretreated lignocellulose. Using furfural-containing precultures, productivity increased to 0.92 g/l/h, with a total lactic acid production of 91.7 g in a 1-l reactor containing 20% W/W DW bagasse. To increase sugar concentrations, bagasse was solubilized with a liquid fraction, obtained directly after acid pretreatment. Solubilizing the bagasse fibres with water increased the average productivity to 1.14 g/l/h, with a total lactic acid production of 84.2 g in a 1-l reactor. Addition of bagasse in two stages reduced viscosity during SSF, resulting in an average productivity in the first 23 h of 2.54 g/l/h, similar to productivities obtained in fermentations using high-grade sugars. Due to fast accumulation of lactic acid, enzyme activity was repressed during two-stage SSF, resulting in a decrease in productivity and a slightly lower total lactic acid production of 75.6 g. Conclusions: In this study, it is shown that an adequate production of lactic acid from lignocellulose was successfully accomplished by a two-stage SSF process, which combines acid-pretreated bagasse, B. coagulans precultivated in the presence of furfural as microorganism, and GC220 as enzyme cocktail. The process may be further improved by enhancing enzyme hydrolysis activities at high lactic acid concentrations
Extracellular vesicles in post-infarct ventricular remodelling
No full textPost-infarct left ventricle remodelling (LVR) is defined as the structural and functional changes of the myocardium occurring after acute myocardial infarction (AMI) and leading to the loss of contractile tissue and subsequently to post-infarct heart failure (HF). LVR affects 30% of patients after AMI, deteriorating the prognosis. At present, no clinical, biochemical and imaging parameters predict which patients will develop LVR and progress to HF. Despite recent progress in pharmacological treatment, mechanical circulatory support and multidisciplinary team management, none of the available treatments entirely prevent, inhibit or reverse LVR. Thus, post-infarct LVR lacks both reliable prediction and effective treatments. Extracellular vesicles (EVs) are biological nanoparticles with a phospholipid bilayer released by probably all eukaryotic and prokaryotic cells to the extracellular environment. EVs are capable of transferring proteins, nucleic acids and signalling ligands between cells, by which they affect the recipient cells. Evidence is accumulating that EVs released from cardiac cells mediate the interplay between cardiomyocytes, fibroblasts, endothelial cells, vascular smooth muscle cells and extracellular matrix underlying LVR. Depending on the cellular origin and concentration of EVs, EVs are either cardioprotective or promote adverse LVR. Hence, EVs from cardiac cells are candidate biomarkers to predict LVR, and are potential drug vehicles of LVR therapy. Here we present the historical background of EVs, introduce EVs derived from cardiac cells, summarize the current evidence on the role of EVs in cardioprotection and adverse LVR, and present the potential clinical applications of EV-based biomarkers and therapeutic
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