8,564 research outputs found
Western Faculty Profile: Dr. Miguel Valvano
No full textDr. Miguel Valvano is a Professor Emeritus in the Department of Microbiology and Immunology at Western University. He also holds an academic Chair of Microbiology and Infectious Diseases at the Centre for Infection and Immunity, Queen’s University Belfast, United Kingdom. His laboratory has become an international leader in molecular research aimed at dissecting key bacterial components that directly interact with host cells to cause infections. His research team focuses on two areas: how lipopolylsaccharide (LPS) assembles on the bacterial cell surface and protects bacteria from host defenses, and characterizing the virulence properties of Burkholderia cepacia, an opportunistic pathogen that causes major health problems in patients suffering from cystic fibrosis. Suzy Kim, a member of WURJ, had the opportunity to interview Dr. Valvano to learn more about his research, his path to Western and his career
Coca cultivation in the town of San Miguel, Putumayo
No full textEl informe es el resultado de una pasantía desarrollada en el Centro de Investigación y Educación Popular Cinep, en el marco del proyecto “San Miguel mira hacia Colombia y el mundo” en el 2007. En las primeras páginas del trabajo, se hace una presentación general del proyecto, destacando sus objetivos, contenidos, desarrollos y resultados, para luego adelantar una reflexión en torno a su la experiencia de la autora en este proceso, cuidando de relievar sus vivencias y aportes como socióloga, tomando como herramienta de análisis el trabajo de campo adelantado en la región de estudio. En la segunda parte del informe se adelanta una caracterización de la zona del Bajo Putumayo, en el contexto de la política de lucha contra las drogas, impulsada por el “Plan Colombia”. En esta perspectiva se ilustra, con elementos históricos y sociológicos, los procesos asociados a la economía de la coca y se analizan las consecuencias del “Plan Colombia” para la región. El trabajo concluye con un análisis de las percepciones que tienen los pobladores y pobladoras del municipio de san Miguel sobre el impacto de la política antidrogas. / Abstract. This report is result of the internship in the proyect “San Miguel mira hacia Colombia y el mundo”, wich was developed in the “Centro de Investigación y Educación Popular” Cinep. The first pages portrays a general presentation of the proyect such as objectives, contents, improvements and results. Then the author makes a reflection about her own experiences in this process based on the fieldwork in the area, including sociologic contributions. In the second part of this report, it is presented a characterization about the Bajo Putumayo area in the context of war against drugs (or antinarcotics police), wich is stimulated by “Plan Colombia”. In this perspective, it shows the process related whit coca economy and analyzes the Plan Colombia consequences in the region. It concludes analyzing of San Miguel settler’s perception on antinarcotics politics and how it impacts in their lives and the area.Pregrad
Raízes integralistas da teoria tridimensional do direito: história das ideias de Miguel Reale (1933-1953)
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Jurídicas, Programa de Pós-Graduação em Direito, Florianópolis, 2014.Este trabalho narra a história das ideias jurídicas de Miguel Reale, jurista e filósofo brasileiro, desde sua adesão ao Integralismo (1933) até a publicação de ?Filosofia do Direito? (1953). A pesquisa centrou-se na interpretação das ?Memórias? do autor (1987), em cotejo com suas obras sobre Integralismo, Teoria do Direito, Teoria do Estado, Filosofia Política e Filosofia do Direito. Também foi realizada pesquisa empírica sobre o diário integralista ?Acção?, dirigido por Reale de 1936 a 1938, cujos exemplares encontram-se microfilmados no Arquivo Público do Estado de São Paulo. As trajetórias intelectual e política do autor foram analisadas conjuntamente, mediante métodos da historiografia e da sociologia dos intelectuais, apoiados nas categorias analíticas de Michel Foucault e Pierre Bourdieu.Abstract : This work is a historical narrative about juridical ideas of Professor Miguel Reale, a brasilian jurist and philosopher, from the time he became involved with the political movement known as ?Integralismo? (1933) until the publication of ?Filosofia do Direito? (1953). This research was based on my interpretation of Reale?s memories (?Memórias?, from 1987), in comparison with his works about ?Integralismo?, Theory of Law, Theory of State, Political Philosophy and Philosophy of Law. In adition to this, it contains an empirical research about ?Acção?, an integralist newspaper directed by Reale between 1936 and 1938. Nowadays, this material is microfilmed in the ?Arquivo Público do Estado de São Paulo?, the State of São Paulo?s Public Archive. The intellectual and political trajectories of the author were analyzed altogether, through the methods of historiography and sociology of intellectuals. Some analytical categories created by Michel Foucault and Pierre Bourdieu were used to achieve this purpose
El Tlacuache Núm. 505 (2012). 505 Año 13 (2012) febrero. El Tlacuache
No full textPagando una deuda de 36 años, Zitlala, 1976-2012 por Luis Miguel Morayta Mendoza. -Los otros en el nosotros, Gustavo y Noé Zapoteco Sideño. -Luis Miguel Morayta Mendoza
An improved bind-n-seq strategy to determine protein-DNA interactions validated using the bacterial transcriptional regulator YipR
No full textBACKGROUND: Interactions between transcription factors and DNA lie at the centre of many biological processes including DNA recombination, replication, repair and transcription. Most bacteria encode diverse proteins that act as transcription factors to regulate various traits. Several technologies for identifying protein-DNA interactions at the genomic level have been developed. Bind-n-seq is a high-throughput in vitro method first deployed to analyse DNA interactions associated with eukaryotic zinc-finger proteins. The method has three steps (i) binding protein to a randomised oligonucleotide DNA target library, (ii) deep sequencing of bound oligonucleotides, and (iii) a computational algorithm to define motifs among the sequences. The classical Bind-n-seq strategy suffers from several limitations including a lengthy wet laboratory protocol and a computational algorithm that is difficult to use. We introduce here an improved, rapid, and simplified Bind-n-seq protocol coupled with a user-friendly downstream data analysis and handling algorithm, which has been optimized for bacterial target proteins. We validate this new protocol by showing the successful characterisation of the DNA-binding specificities of YipR (YajQ interacting protein regulator), a well-known transcriptional regulator of virulence genes in the bacterial phytopathogen Xanthomonas campestris pv. campestris (Xcc).RESULTS: The improved Bind-n-seq approach identified several DNA binding motif sequences for YipR, in particular the CCCTCTC motif, which were located in the promoter regions of 1320 Xcc genes. Informatics analysis revealed that many of these genes regulate functions associated with virulence, motility, and biofilm formation and included genes previously found involved in virulence. Additionally, electromobility shift assays show that YipR binds to the promoter region of XC_2633 in a CCCTCTC motif-dependent manner.CONCLUSION: We present a new and rapid Bind-n-seq protocol that should be useful to investigate DNA-binding proteins in bacteria. The analysis of YipR DNA binding using this protocol identifies a novel DNA sequence motif in the promoter regions of target genes that define the YipR regulon.</p
Acercamiento al ideario estético de Miguel de Unamuno
No full text[ES] Recopilación de diversas consideraciones presentes en la obra de Miguel de Unamuno relacionadas con la percepción y la emoción estéticas, desde el paisaje a las distintas manifestaciones de la creación artística, que permiten al autor proponernos un breve ideario estético unamuniano.[EN] The author proffers an overview of Miguel de Unamuno's ideological approach to aestethics based on a brief compilation of the numerous reflections about aesthetic emotions and perceptions present in his work when confronted with landscape or with the various forms of artistic expression
Un homenaje a Miguel Reale (1910-2006)
No full textThis article pays homage to the intellectual journey of Miguel REALE and to the unquestionable relevance of his contributions to philosophy of law. Not only did REALE combine the duality between thought and action in his philosophy, but also their mutual implication, convinced as he was that the starting point for the practice of philosophy of law resides in the jurist’s experience. Among his most remarkable contributions the author points out REALE’S three-dimensionalism, in which the sociological perspective of the fact, the philosophical perspective of the value of the fair and the perspective of the norm in the scope of legal dogmatics are brought together in a continuous interrelationship and interdependence. In addition, the author shows some contributions in which REALE’S three-dimensionalism offers new means of understanding.Este artículo es un homenaje a la trayectoria intelectual de Miguel REALE y a la indiscutible trascendencia de sus contribuciones a la Filosofía del Derecho. En su filosofía, REALE combinó la dualidad, pero también la mutua implicación, entre pensamiento y acción, convencido de que el punto de partida para hacer Filosofía del Derecho era desde la experiencia del jurista. Entre sus aportaciones más destacadas, el autor destaca el tridimensionalismo de REALE en el que se conjugan la perspectiva sociológica del hecho, la perspectiva filosófica del valor de lo justo y la perspectiva de la norma en el ámbito de la dogmática jurídica, en una continua interrelación e interdependencia. Adicionalmente, el autor muestra algunas contribuciones en las que el tridimensionalismo de REALE ofrece nuevas vías de entendimiento
El Tlacuache Núm. 430 (2010). 430 Año 10 (2010) agosto. El Tlacuache
No full textLa Revolución en la Huasteca Hidalguense, un espejo para revisar la experiencia indígena zapatista morelense por Luis Miguel Morayta Mendoza. -¿Quiénes son chamanes en el estado de Morelos? por Alfredo Paulo Maya, Raúl Francisco González Quezad
El Tlacuache Núm. 605 (2014). 605 Año 13 (2014) enero. El Tlacuache
No full textPorfesora Amanda Verónica Barberi, una impulsora incomparable de las culturas locales, en el ámbito de la educación básica, nos dejó por Luis Miguel Morayta Mendoza. - Vida cotidiana de jornaleros migrantes en un campamento agrícola en Morelos por Adriana Saldaña Ramírez
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