1,721,023 research outputs found
Hodgkin's disease mimicking suppurative lymphadenitis: a possible pitfall in fine-needle aspiration biopsy cytology.
No full textWe report one case of nodular sclerosing Hodgkin's disease with uncommon pseudosuppurative presentations in fine-needle aspiration biopsy (FNAB) samples and on histopathologic material. The criteria for cytologic diagnosis include atypical mononuclear cells and diagnostic SR cells in the purulent exudate. This lesion is considered a possible pitfall in FNAB cytology of Hodgkin's disease
IMPACT OF ENDOSCOPIC ULTRASOUND-GUIDED FINE NEEDLE ASPIRATION IN MEDIASTINAL AND LUNG LESIONS: A COMBINED EXPERIENCE OF DIFFERENT INSTITUTIONS
No full textBACKGROUND: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is an established procedure in lung cancer (LC) staging and in the diagnosis of mediastinal masses. Most of the experiences reported refer to single specialized centers where dedicated teams of endoscopists and pathologists perform the procedure. We report the EUS-FNA experience of a cooperation group involving clinicians and cytopathologists from three hospitals. METHODS: Fifthy-seven consecutive EUS-FNA of mediastinal nodes in LC patients, 8 mediastinal and 2 sub-diaphragmatic masses were collected in three years. EUS-FNA was performed by two endoscopists and three experienced pathologists; on-site evaluation was performed in all cases by the three cytopathologists. Lymph node negative cases underwent surgery, which confirmed the cytological diagnoses but also detected two false negatives. Four of the 10 EUS diagnoses of mediastinal masses were histologically confirmed. All EUS diagnoses were blindly reviewed by three pathologists to assess intra and interpersonal reproducibility. RESULTS: FNA-EUS diagnoses were: 10 inadequate (17%), 10 negative (17%), 4 suspicious ( 7% ) and 33 positive (59%). Diagnoses of mediastinal and sub-diaphragmatic masses were: relapse of LC (3), mesenchimal tumour NOS (3), gastrointestinal stromal tumor (GIST) (1), esophageal carcinoma (2) and paraganglioma (1). Attained sensitivity and specificity were 85% and 100% with an high interpersonal diagnostic reproducibility (P<0.5). CONCLUSION: The sensitivity and specificity attained were similar to those reported in the literature suggesting that experienced cytopathologists and endoscopists from different Institutions can employ the same procedure reaching comparable results
EUROCYTOLOGY.EU: Lymph node cytology
No full textLymph nodes were probably the first organs to be investigated by fine needle cytology (FNC) and the last in which this technique has been accepted by clinicians as a useful diagnostic procedure. Risk of seeding, false negatives, failure to discriminate between reactive hyperplasia and lymphoma, have been put forward as a limitation or useless of FNC. Nonetheless, as this technique is fast, inexpensive and effective, it is often required by general practitioners, specialists and surgeons as first diagnostic approach to lymph nodes; moreover, ancillary techniques such as immuocytochemistry, flow cytometry, fluorescence in situ hybridization and molecular techniques are successfully employed on cytological samples, conferring to FNC of lymph nodes credibility and a definite role in the diagnosis of lymphadenopaties. There are exhaustive books and excellent articles providing lymph nodal cytopathologists with the necessary knowledge of the different pathologies encountered, and the limitations of the technique; however, a small dose of audacity is also a useful complement when practicing this field of cytology
Lymph node and lymphoid organs fine needle aspiration cytology: historical background.
No full textLymph node has probably been the first target of Fine Needle Cytology (FNC) and among the latest to be accepted as an affordable diagnostic procedure. In 1912, dr. Hirschfeld performed FNC to diagnose cutaneous lymphomas and other tumours.
Subsequently FNC was used to diagnose lymphoblastoma and splenic FNC to diagnose leishmaniasis on Romanowsky-stained smears. One of the first systematic study on lymph node FNC was then performed at John Hopkins Hospital, in Baltimore (USA)
using FNC and Romanowsky stain on air-dried smears. In the twenties, two independent groups from Memorial Hospital (New York, USA), worked on FNC of a large scale of different human pathologies. One of this study reported 1,405 diagnoses of cancer and other diseases by means of FNC, mainly performed on lymph
nodes (662 cases). In the sixties, at the Karolinska Hospital (Stockholm, Sweden) a group of cytopathologists started a Cytopathology Service available to the whole Institution, which exploited all fields of FNC. Since then, the procedure spread all over the word and nowadays it is routinely used for the diagnosis of different organs and pathologies including lymph node. Distinguished cytopathologists have worked on lymph nodal FNC producing significant advances and highlighting advantages and inevitable limitations of the technique. Despite some persistent criticism, FNC is a generally accepted procedure in the first
diagnosis of lymph nodes enlargement. Moreover, numerous studies have demonstrated that vital cells obtained by FNC are excellent samples suitable for molecular evaluation, offering new challenging application to lymph node FNC
The role of morphometry in the cytology of pleomorphic adenomas of the salivary glands
No full textA morphometric analysis was performed on cytologic smears (FNAB) of 5 cases of pleomorphic adenoma that recurred after the first diagnosis and on 5 ordinary pleomorphic adenoma. The considered parameters were as follows: nuclear area,
perimeter, maximum diameter, feret x, feret y, F shaped and D circle. A statistical analysis was performed between the two groups for all the considered parameters using a two-sided Student's t-test for independent samples. The test showed significant differences of the parameter nuclear area. The authors
conclude that a morphometric assessment may bring a significant contribution in the knowledge and prediction of the outcome of pleomorphic adenoma
Fine needle aspiration biopsy of hepatic focal fatty change. A report of two cases.
No full textBACKGROUND: In rare instances, hepatic steatosis produces a circumscribed, nodular lesion described as focal fatty liver change (FFLC). The ultrasonographic and computed tomographic patterns are those of an isointense or hyperechoic nodule, sometimes simultating metastasis.
CASES: Fine needle aspiration biopsy was performed under ultrasonographic control in two men aged 65 and 67 years who had previously undergone emicolectomy and
gastrectomy for adenocarcinoma. Routine hepatic ultrasound showed solitary nodules, of 3 and 4 cm in diameter. The microscopic patterns were similar and highly cellular in both cases. Cells were isolated or organized in sheets and
characterized by large, intracytoplasmic, clear vacuoles that displaced nuclei to the periphery of the cells, flattening them against the cytoplasmic membrane and giving these cells a signet-ring appearance. Nuclei were generally round and
nucleolated or dense and hyperchromatic when flattened onto the cytoplasmic membrane. Normal hepatocytes were interspersed in the background, and in some areas of the slides hepatocytes with one or more small intracytoplasmic vacuoles with cytologic features intermediate between those of vacuolated cells and normal hepatocytes were present. Digested periodic acid-Schiff staining, performed on
destained, fixed smears, gave negative results. The cytologic diagnosis was FFLC. Clinical and echographic follow-up confirmed the cytologic diagnosis. CONCLUSION: The ultrasonographic and microscopic features of FFLC may mimic those of metastasis. A proper cytologic diagnosis may contribute to the diagnostic workup of these rare lesions
- …
