1,721,183 research outputs found
Performances of cytology and flow cytometry to predict diagnosis of canine lymphoma and subtypes
Full text linkCytology plays a central role in the diagnostic algorythm for canine lymphoma. Canine lymphoma is a great
candidate to be sampled via fine needle aspiratebiopsy since it is mainly a multicentric disease, involvement of
superficial nodes is frequent and the architectural histological pattern is diffuse in most cases. Accuracy may be
implemented by ancillary techniques such as flow cytometry (FC) and immunocytochemistry (ICC). However, the
diagnostic performances of cytology alone with or without some ancillary techniques is not completely elucidated
in veterinary medicine. In a recent metanalysis in humans, cytology alone showed a sensitivity of 87% and a
specificity of 97% in the identification of lymphoma vs non-lymphoma. This values increased by adding FC or ICC.
However, no data on the performances in identifying specific lymphoma subtypes via cytology were described.
In dogs the current classifications used for canine lymphoma are the updated Kiel and the WHO classifications. The
updated Kiel classification is mainly a cytological scheme based on morphological aspects and it demonstrated a
fair agreement among readers in a previous study. In contrast, WHO is mainly based on histological criteria but
previous papers suggest a possible correlation between these two diagnostic schemes. We recently evaluated
the diagnostic performances and repeatibility of cytology alone for 1) predicting lymphoma vs non-lymphoma; 2)
predicting grade and subtype and 3) predicting WHO subtype.
Cytology showed good performances (sensitivity 92.6%, specificity 89.4%) and repeatibilities in predicting
lymphoma vs non-lymphoma. As expected this values decrease for the evaluation of grade and phenotype, and
for WHO subtype. The major diagnostic challenges were the identification of the grade of B cell lymphomas
and the identification of the immunophenotype for high grade lymphomas with many B cell lymphoma cases
minsinterpreted as T cell.
Flow cytometry may easily help to definitely diagnose the immunophenotype thus solving this issue. On the
other side it may also be useful to discriminate between high and low grade lymphomas by possibly evaluating
the percentage of KI67 positive cells. Further studies on the diagnostic performance of cytology + FC for canine
lymphoma are needed and currently in process
La visione del Territorio. Intervista a Silvia Lanzani (Assessore alla Provincia di Bergamo con delega alla Pianificazione Territoriale)
No full textDomande poste all'Assessore alla Provincia di Bergamo, Silvia Lanzani, sull'immagine del territorio che i governatori provinciali vogliono dare alle generazioni future.
Una riflessione sul disegno del territorio tra realtà (stato di fatto) e potenzialità (quadro desiderabile
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
CLINICAL PATHOLOGICAL FEATURES OF CANINE HEMATOPOIETIC NEOPLASMS ASSESSED VIA FLOW CYTOMETRY
Full text linkFlow cytometry (FC) is an advanced diagnostic technique widely used in human medicine to confirm, classify and stage hematopoietic neoplasms (HNs): it supplies an objective evaluation of the size and internal complexity of the cells and identifies the antigenic pattern expressed by each cell, which is specific for cellular lineage and maturative stage. FC is presently spreading also in veterinary medicine and is mostly used to confirm the diagnosis and determine the immunophenotype of canine HNs.
Aim of this study was to apply FC to the study of specific clinical pathological features of canine HNs. In particular, we focused on three points:
1) staging of diffuse large B-cell lymphoma. We validated a FC technique to assess peripheral blood and bone marrow infiltration by neoplastic cells and proved its prognostic value.
2) antigen aberrancies. We investigated the prognostic role of specific antigen aberrancies in canine lymphomas and found the dogs with CD4+CD8+ T-cell lymphomas had a poorer prognosis compared to other T-cell aberrant lymphomas. In addition, we highlighted an important diagnostic role for antigen aberrancies in canine small clear cell lymphoma.
3) CD44 expression. We proved that CD44, which has a role in the pathogenesis and dissemination of many human and canine neoplasms, is expressed at different degrees on neoplastic cells from different canine HNs, with acute leukemias showing the highest degree of expression.
Overall, our results suggest that FC can be a useful tool not only to confirm the diagnosis and assess the immunophenotype of canine HNs, but also to assess clinical pathological aspects and additional prognostic parameters specific for the neoplastic subtype identified
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Linfoma stadio V: relazione tra infiltrazione midollare e citopenie periferiche nel cane
No full textLa stadiazione clinica WHO del linfoma canino prevede la divisione in 5 stadi in base al coinvolgimento di linfonodi (I-III), fegato/milza (IV), sangue periferico, midollo o altri organi (V). Le linee guida del VCOG (Veterinary Cooperative Oncology Group) consigliano di valutare il midollo solo nei cani con citopenia, mancando linee guida per la tecnica di valutazione e per le scelte terapeutiche in base all’infiltrazione. Alcuni autori hanno però segnalato che stadi inferiori al V possono avere tempi di sopravvivenza migliori. Scopo di questo studio retrospettivo è valutare la correlazione tra alterazioni ematologiche e infiltrazione midollare valutata tramite citofluorimetria in cani con linfoma B a grandi cellule. Sono stati inclusi 60 cani per cui fossero disponibili esame emocromocitometrico, striscio ematico e midollare e citofluorimetria di linfonodo, sangue e midollo osseo. Il livello di infiltrazione è stato definito come percentuale di cellule CD21+ ad alto forward scatter. Alterazioni periferiche erano presenti in 30 cani (17 anemici, 15 trombocitopenici, 11 con linfocitosi, 5 con cellule atipiche e 11 con infiltrazione >15%). 14 casi avevano alterazioni ematologiche multiple.
L’infiltrazione midollare era 10,09% ± 16,14 (mediana 3,05); in 43 casi era 20%. L’infiltrazione midollare risulta significativamente associata a trombocitopenia, linfocitosi e infiltrazione ematica (p=0,00), ma non ad anemia o cellule atipiche (p=0,56; p=0,36). I nostri risultati suggeriscono l’opportunità di valutare sempre il midollo, anche se trombocitopenia o
linfocitosi possono indicare la presenza di infiltrazione
Between conservation and development: project indications
No full textIpotesi di trentadue indicazioni progettuali per la valorizzazione del quartiere Greco-Ortodosso dell'Annunciazione a Nazareth, basate sui principi di valorizzazione ispirati al Codice dei Beni Culturali Italiano (D.Lgs. 42/2004). Lo scopo delle indicazioni progettuali è di migliorare il paesaggio degradato del quartiere e, nel contempo, elevare la leggibilità dei suoi valori storico-culturali rimodulandone conoscenza, visibilità, accessibilità, in modo compatibile con le crescenti esigenze turistiche.
All'interno delle trentadue indicazioni progettuali, sono state sviluppate a cura di M. Merlo, L. Astegno, indicazioni per il restauro conservativo della settecentesca Chiesa Ortodossa dell’Annunciazione che contiene, secondo la tradizione, il Pozzo di Maria
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