596 research outputs found
Dicionário de escritoras catarinenses
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão, Programa de Pós-Graduação em Literatura, Florianópolis, 2011O ato de escrever foi, por muitos anos, considerado de domínio masculino. Aos poucos, as mulheres foram transgredindo normas e conquistando também esse espaço; contudo, a escrita de autoria feminina não esteve muito presente na formação da literatura brasileira e, consequentemente, no cânone literário. Em Santa Catarina (e na maior parte do país), o aparecimento efetivo da literatura escrita por mulheres se dá no século XIX. Diversas foram as escritoras que publicaram seus textos (basicamente poemas) em jornais; no entanto, suas publicações em livro eram muito limitadas. A partir do século XX, principalmente após os anos 1970, motivadas pelo feminismo e seus consequentes reflexos políticos e sociais, as mulheres intensificam sua vida intelectual e começam a ter maior acesso ao mundo das publicações. A fim de demonstrar essa tese, esta pesquisa teve como objetivo organizar um dicionário literário de mulheres catarinenses, contemplando os mais diversos gêneros, como: poesia, contos, crônicas, romances, memórias e literatura infanto-juvenil. Para isso, foi realizada uma pesquisa qualitativa de caráter exploratório envolvendo pesquisa de campo em diversas cidades catarinenses. Ao todo, foram organizados 401 verbetes que incluem a biografia e bibliografia das escritoras. Os resultados comprovaram que o gênero predominante na escrita das mulheres em Santa Catarina é a poesia e que as cidades catarinenses que mais publicam são Florianópolis, Blumenau, Joinville, Itajaí e Lages.L´acte d´écrire a été, pendant longtemps, consideré comme un acte masculin. Peu à peu, les femmes ont essayé de changer les normes et ont commencé a conquérir le droit de participer à la littérature; par contre, l´écriture féminine n´a pas été trop présente à la formation de la littérature brésilienne et, par conséquent, au canon littéraire. À Santa Catarina (ainsi que dans la plus grande partie du pays), les premières manifestations de la littérature féminine sont du XIX siècle. Les femmes ont été nombreuses à publier leurs textes (surtout des poèmes) dans les journaux mais en livres cela a été bien limité. A partir du XXème siècle, surtout aprés les années 1970, motiveés par le mouvement féministe et ses reflets politiques et sociaux, les femmes ont intensifié leur participation à la vie intellectuelle et ont commencé à publier beaucoup plus. Pour valider cette thèse, la recherche a eu como objectif celui d´organizer un dictionnaire litéraire des écrivains "catarinenses", qui a accepté plusieures genres, comme, par exemple: poèmes, nouvelles, contes, romans, mémoires. Pour cela, une recherche qualitative exploratoire a eté accomplie dans plusieurs villes de Santa Catarina. Nous avons organisé 401 entrées dans le dictionnaire qui comprennent la biographie et la bibliographie de l´oeuvre de chaque auteur. Les résultats montrent que le genre dominant dans l´écriture féminine à Santa Catarina est la poésie et que les villes avec le plus grand nombres d´auteurs sont: Florianópolis, Blumenau, Joinville, Itajaí e Lages
Incidence and predictors of sudden death in patients with cardiac amyloidosis
No full textIntroductionAlthough sudden death (SD) is a recognized complication of cardiac amyloidosis, there is scarce data about its incidence, mechanisms, and predictors. The aim of this study was to describe incidence of SD and to analyze possible risk factors.MethodsConsecutive patients with ATTR or AL cardiac amyloidosis evaluated at two European centers were identified. SD was defined as unexpected death in clinically stable patients. Cox proportional hazard regression was performed to assess risk factors in univariate analysis. Those statistically significant were then assessed through age-adjusted multivariate analysis.ResultsAnalysis included 784 patients, 569 with ATTR amyloidosis (mean age 74.1 +/- 12.1 years) and 215 with AL amyloidosis (mean age 64.5 +/- 10.8 years). After a median follow-up of 1.9 years, SD rate at 2 years was 1.8% in ATTR. Previous pacemaker implantation (PPM) was associated with increased risk after age-adjusted analysis (HR 4.97; 95%CI: 1.39-17.7; p = 0.01). SD rate in AL amyloidosis patients at 2 years was 8.0% after a median follow-up of 1.2 years. Betablockers and NYHA III-IV were independently associated with an increased risk after age-adjusted multivariate analysis (HR 7.06 95%CI (2.31-21.5) p = 0.001) and (HR 4.56 95%CI (1.51-13.8) p = 0.007) respectively.ConclusionsSD is more frequent in AL than in ATTR cardiac amyloidosis. SD is associated with different risk factors in both entities
Genetic analysis, in silico prediction, and family segregation in long QT syndrome
No full textThe heritable cardiovascular disorder long QT syndrome (LQTS), characterized by prolongation of the QT interval on electrocardiogram, carries a high risk of sudden cardiac death. We sought to add new data to the existing knowledge of genetic mutations contributing to LQTS to both expand our understanding of its genetic basis and assess the value of genetic testing in clinical decision-making. Direct sequencing of the five major contributing genes, KCNQ1, KCNH2, SCN5A, KCNE1, and KCNE2, was performed in a cohort of 115 non-related LQTS patients. Pathogenicity of the variants was analyzed using family segregation, allele frequency from public databases, conservation analysis, and Condel and Provean in silico predictors. Phenotype-genotype correlations were analyzed statistically. Sequencing identified 36 previously described and 18 novel mutations. In 51.3% of the index cases, mutations were found, mostly in KCNQ1, KCNH2, and SCN5A; 5.2% of cases had multiple mutations. Pathogenicity analysis revealed 39 mutations as likely pathogenic, 12 as VUS, and 3 as non-pathogenic. Clinical analysis revealed that 75.6% of patients with QTc≥500 ms were genetically confirmed. Our results support the use of genetic testing of KCNQ1, KCNH2, and SCN5A as part of the diagnosis of LQTS and to help identify relatives at risk of SCD. Further, the genetic tools appear more valuable as disease severity increases. However, the identification of genetic variations in the clinical investigation of single patients using bioinformatic tools can produce erroneous conclusions regarding pathogenicity. Therefore segregation studies are key to determining causality.European Journal of Human Genetics advance online publication, 26 March 2014; doi:10.1038/ejhg.2014.54
Feature extraction based on timesingularity multifractal spectrum distribution in intracardiac atrial fibrillation signals
No full textNon-linear analysis of electrograms (EGM) has been proposed as a tool to detect critical conduction sites (e.g., rotors vortex, multiple wavefronts) in atrial fibrillation (AF). Likewise, studies have shown that multifractal analysis is useful to detect critical activity in EGM signals. However, the multifractal spectrum does not consider the temporal information. There is a new mathematical formalism to overcome this limitation: the timesingularity multifractal spectrum distribution (TS-MFSD), which involves the time variation of the spectrum. In this manuscript, we describe the methodology to compute the TS-MFSD from EGM signals. Moreover, we propose a methodology to extract features from time-singularity spectrum and from singularity energy spectrum (SES). We tested the features in an EGM database labeled by experts as: non-fragmented, discrete fragmented potentials, disorganized activity, and continuous activity. We tested the area under the receiver operating characteristic (ROC) curve. The proposed features achieve an area under the ROC curve of 95.17% when detecting signals with continuous activity. These results outperform those reported using multifractal analysis. To our knowledge, this is the first work that report the use of TS-MFSD in biomedical signals and our findings suggest that time-singularity has the potential to be used in the study of non-stationary behavior of EGM signals in AF
Membrane expression of Na<sub>v</sub>1.5 channel is not affected by I890T.
No full text<p>Western blot detection of Na<sub>v</sub>1.5 and Na<sup>+</sup>/K<sup>+</sup> ATPase proteins performed after cell surface biotinylation from WT and I890T cells. (A) Image shows the bands obtained for Na<sub>v</sub>1.5 and Na<sup>+</sup>/K<sup>+</sup> ATPase, in 6 independent experiments from cells expressing either WT or I890T. Position of the markers is shown on the left side. Numbers correspond to each experiment. (B) Bar graph depicts the Na<sub>v</sub>1.5 intensity values normalized by the Na<sub>v</sub>1.5 WT intensity values (n = 6). Na<sub>v</sub>1.5 I890T intensity values were previously corrected by multiplying the raw integrated density values by the ratio between the WT and I890T Na<sup>+</sup>/K<sup>+</sup> ATPase integrated density values. Note that for experiment number 5, the ratio between I890T and WT was calculated from the average of the intensity values obtained for the two samples of each condition. (C) Bar graph shows the average intensity values (expressed as mean ± SE) obtained in (B).</p
Fluye Emoción: Aporte al bienestar emocional de los jóvenes en Bogotá que fue deteriorado debido al aislamiento por la pandemia de Covid-19
No full textEste proyecto aborda el deterioro del bienestar emocional de los
jóvenes en Bogotá generado debido al aislamiento por la pandemia
de COVID-19, por lo tanto, el objetivo general es favorecer el
bienestar emocional de los jóvenes por medio de un producto
multimedial, que permita el mejoramiento de la calidad de vida. La
metodología tiene seis etapas: mapear contexto, empatizar, definir
necesidades, explorar ideas, prototipar y testear, en las cuales se
desarrollan herramientas como entrevistas, esquemas y prototipos. A
partir de lo anterior se plantea Fluye Emoción, que consiste en videos
informativos sobre el tema objeto de estudio dentro de dos enfoques:
inteligencia emocional y hábitos saludables de autocuidado. Los
hallazgos más importantes son que el usuario identifica en sí mismo
afectaciones sobre su bienestar emocional, por tal motivo quiere
saber del tema, presenta interés por consumir el producto y opina que
este cumple con el objetivo general y con los requerimientos de ser
claro, útil y dinámico. Se concluye que se logra favorecer el bienestar
emocional de los jóvenes en Bogotá con Fluye Emoción.This project addresses the deterioration of the emotional well-being of
young people in Bogotá generated due to the isolation due to the
COVID-19 pandemic, therefore, the general objective is to favor the
emotional well-being of young people through a multimedia product,
which allows the improvement of the quality of life. The methodology
has six stages: mapping context, empathizing, defining needs,
exploring ideas, prototyping and testing, in which tools such as
interviews, schemes and prototypes are developed. Based on the
above, Fluye Emoción is proposed, which consists of informative
videos on the subject under study within two approaches: emotional
intelligence and healthy habits of self-care. The most important
findings are that the user identifies in himself affectations on his
emotional well-being, for this reason he wants to know the subject,
presents interest in consuming the product and believes that it meets
the general objective and with the requirements of being clear, useful
and dynamic. It is concluded that it is possible to favor the emotional
well-being of young people in Bogotá with Fluye Emoción.Aval del Proyecto 5
Dedicatoria 9
Agradecimientos 11
Resumen 13
Abstract 14
Tabla de contenido 16
Listado de figuras 19
Listado de tablas 22
Listado de anexos 23
1. Formulación del proyecto 24
1.1 Introducción 24
1.2 Justificación 26
1.3 Definición del problema 30
1.4 Hipótesis de la investigación 35
1.4.1 Hipótesis explicativa 35
1.4.1 Hipótesis propositiva 35
1.5 Objetivos 35
1.5.1 Objetivo general 37
1.5.2 Objetivos específicos 37
1.6 Planteamiento metodológico 38
1.7 Alcances y limitaciones 41
2. Base teórica del proyecto 44
2.1 Marco referencial 44
2.1.1 Antecedentes 44
2.1.2 Marco teórico contextual 49
2.1.3 Marco teórico disciplinar 59
2.1.4 Marco conceptual 70
2.1.5 Marco legal 75
2.2 Estado del arte 78
2.2.1 Aplicaciones móviles 79
2.2.2 Videos informativos en YouTube 89
2.2.3 Estrategias de contenidos en Instagram 97
2.2.4 Estrategias de contenidos en TikTok 105
2.3 Caracterización de usuario 116
3. Desarrollo de la metodología, análisis y presentación de
resultados 124
3.1 Criterios de diseño 124
3.1.1 Árbol de objetivos de diseño 127
3.1.2 Requerimientos y determinantes de diseño 130
3.2 Hipótesis de producto 135
3.3 Desarrollo y análisis Etapa 1: Mapear contexto 138
3.4 Desarrollo y análisis Etapa 2: Empatizar 141
3.5 Desarrollo y análisis Etapa 3: Definir necesidades 147
3.6 Desarrollo y análisis Etapa 4: Explorar ideas 151
3.7 Desarrollo y análisis Etapa 5: Prototipar 158
3.8 Desarrollo y análisis Etapa 6: Testear 163
3.9 Resultados de los testeos 164
3.9.1 Primer testeo 164
3.9.2 Segundo testeo 171
3.9.3 Tercer testeo 175
3.10 Prestaciones del producto 179
3.10.1 Aspectos morfológicos 179
3.10.2 Aspectos técnico-funcionales 185
3.10.3 Aspectos de usabilidad 186
4. Conclusiones 187
4.1 Conclusiones 187
4.2 Estrategia de mercado 190
4.2.1 Segmentos de cliente 191
4.2.2 Propuesta de valor 192
4.2.3 Canales 194
4.2.4 Relaciones con los clientes 195
4.2.5 Fuentes de ingresos 196
4.2.6 Actividades clave 197
4.2.7 Recursos clave 199
4.2.8 Socios clave 201
4.2.9 Estructura de costes 202
4.3 Consideraciones 204
Referencias 206
Anexos 219PregradoDiseñador(a) Digital y MultimediaProductos audiovisuale
Author Correction: A portrait of the Higgs boson by the CMS experiment ten years after the discovery
No full textIn the version of this article initially published, CMS Collaboration author names, affiliations and acknowledgements were omitted and have now been included in the HTML and PDF versions of the articl
I890 is a conserved aminoacid, located in the intramembrane pore region of Na<sub>v</sub>1.5 DII.
No full text<p>(A) Sequence alignment of the pore modules of human Na<sub>v</sub>1.5 channel (DII) and Na<sub>v</sub><i>Ab</i>. Identical aminoacids are highlighted in grey. Isoleucine-890 is marked with a dark box. Similar aminoacids are included inside light boxes and dots identify insertions (lower panel). Sequence alignment of human voltage-gated sodium channel α-subunit family members and of Na<sub>v</sub>1.5 channels of different species, upper and middle panels, respectively. The position of the first amino acid of each sequence is indicated on the left side, and the reference for each protein according to Uniprot is shown at the right side. (B) Partial view of the CPHmodel showing the pore module of DII of Na<sub>v</sub>1.5 channel (in green), based on the coordinates of Na<sub>v</sub><i>Ab</i> channel (in red). I890<sub>Nav1.5</sub> and T169<sub>Nav<i>Ab</i></sub> are located in the middle of P1-helix and highlighted in blue and magenta, respectively. View from the interior side of the pore. (C) Na<sub>v</sub>1.5 channel scheme. The relative position of the I890T mutation in the S5–S6 loop of domain II (DII) is indicated with an arrow.</p
Distribución tiempo singularidad del espectro multifractal para el análisis de electrogramas intracardiaco en fibrilación atrial
No full textNon-linear analysis of electrograms (EGM) has been proposed as a tool to detect critical conduction sites (e.g., rotors vortex, multiple wavefronts) in atrial fibrillation (AF). Likewise, studies have shown that multifractal analysis is useful to detect critical activity in EGM signals. However, the multifractal spectrum does not consider the temporal information. There is a new mathematical formalism to overcome this limitation: the time-singularity multifractal spectrum distribution (TS-MFSD), which involves the time variation of the spectrum. In this manuscript, we describe the methodology to compute the TS-MFSD from EGM signals. Moreover, we propose a methodology to extract features from time-singularity spectrum and from singularity energy spectrum (SES). We tested the features in an EGM database labeled by experts as: non-fragmented, discrete fragmented potentials, disorganized activity, and continuous activity. We tested the area under the receiver operating characteristic (ROC) curve. The proposed features achieve an area under the ROC curve of 95.17% when detecting signals with continuous activity. These results outperform those reported using multifractal analysis. To our knowledge, this is the first work that report the use of TS-MFSD in biomedical signals and our findings suggest that time-singularity has the potential to be used in the study of non-stationary behavior of EGM signals in AF.El análisis de la dinámica no lineal de señales de Electrogramas Intracardiacos (EGM) ha sido propuesto como una herramienta para detectar sitios críticos de conducción eléctrica (ejm: rotores o múltiples frentes de onda) en fibrilación auricular (AF). Estudios previos han mostrado que el análisis multifractal puede ser de utilidad para detectar actividad crítica en la señal EGM. A pesar de esto, el análisis multifractal no considera la información temporal de la señal. Existe un nuevo formalismo matemático para superar esta limitación, el cual es llamado Distribución Tiempo-Singularidad del Espectro Multifractal (TS-MFSD), que involucra la variación en el tiempo del espectro. Este artículo describe una nueva metodología para calcular características a partir del TS-MFSD en señales EGM. Nosotros evaluamos los métodos descritos en una base de datos de EGM etiquetada por expertos en cuatro clases: no fragmentada, potenciales fragmentados discretos, actividad desorganizada y actividad continua. Para evaluar el rendimiento se calculó el área bajo la curva ROC. El mejor resultado de las características propuestas alcanzó un área bajo la curva ROC de 95.17% en la detección de señales con actividad continua. Este resultado supera los reportados mediante la utilización del análisis multifractal. Hasta donde sabemos, este es el primer trabajo que reporta la utilización de la TS-MFSD en señales biomédicas, y nuestros resultados sugieren que el análisis Tiempo-Singularidad tiene el potencial para estudiar el comportamiento no estacionario de las señales EGM en AF
Factores de riesgo de infecciones de dispositivos cardiacos implantables: estudio de casos y controles anidado
No full text- …
