8 research outputs found
UJI AKTIVITAS EKSTRAK ETANOL BATANG DAN DAUN SIRIH CINA (Peperomia pellucida (L.) Kunth) TERHADAP Trichophyton rubrum: Antifungal Activity of Ethanolic Stem and Leaf Extracts of Peperomia pellucida (L.) Kunth against Trichophyton rubrum
No full textDermatofitosis adalah jenis infeksi kulit yang disebabkan oleh fungi dermatofita, salah satu penyebab yang paling umum adalah spesies Trichophyton rubrum. Tanaman sirih cina (Peperomia pellucida (L.) Kunth) merupakan bahan alam yang mengandung senyawa antijamur seperti alkaloid, flavonoid, saponin, tanin, dan terpenoid. Keefektifan penggunaan kombinasi batang dan daun sirih cina oleh masyarakat perlu dikaji secara saintifik. Oleh karena itu, penelitian ini ditujukan untuk mengetahui aktivitas antifungi dari ekstrak etanol batang dan daun Peperomia pellucida (L.) Kunth terhadap Trichophyton rubrum. Penelitian ini terdiri atas 14 kelompok perlakuan dengan 5 kali pengulangan. Aktivitas antifungi diuji menggunakan metode cup-plate dengan variasi konsentrasi 10%, 15%, 25%, 40%, 60%, 85% dari setiap ekstrak batang dan daun sirih cina. Uji ANOVA Dua Arah menunjukkan terdapat perbedaan diameter zona hambat yang nyata akibat pengaruh dari jenis ekstrak dan kelompok perlakuan. Uji lanjut Duncan menunjukkan bahwa ekstrak batang memberikan aktivitas antifungi yang lebih baik dari pada ekstrak daun dengan urutan daya hambat tertinggi hingga terendah yakni konsentrasi 85%, 60%, 40%, 25%, 15%, dan 10%. Dengan demikian dapat dinyatakan bahwa semakin tinggi konsentrasi maka semakin besar pula daya atau zona hambat yang terbentuk.Dermatofitosis adalah jenis infeksi kulit yang disebabkan oleh fungi dermatofita, salah satu penyebab yang paling umum adalah spesies Trichophyton rubrum. Tanaman sirih cina (Peperomia pellucida (L.) Kunth) merupakan bahan alam yang mengandung senyawa antijamur seperti alkaloid, flavonoid, saponin, tanin, dan terpenoid. Keefektifan penggunaan kombinasi batang dan daun sirih cina oleh masyarakat perlu dikaji secara saintifik. Oleh karena itu, penelitian ini ditujukan untuk mengetahui aktivitas antifungi dari ekstrak etanol batang dan daun Peperomia pellucida (L.) Kunth terhadap Trichophyton rubrum. Penelitian ini terdiri atas 14 kelompok perlakuan dengan 5 kali pengulangan. Aktivitas antifungi diuji menggunakan metode cup-plate dengan variasi konsentrasi 10%, 15%, 25%, 40%, 60%, 85% dari setiap ekstrak batang dan daun sirih cina. Uji ANOVA Dua Arah menunjukkan terdapat perbedaan diameter zona hambat yang nyata akibat pengaruh dari jenis ekstrak dan kelompok perlakuan. Uji lanjut Duncan menunjukkan bahwa ekstrak batang memberikan aktivitas antifungi yang lebih baik dari pada ekstrak daun dengan urutan daya hambat tertinggi hingga terendah yakni konsentrasi 85%, 60%, 40%, 25%, 15%, dan 10%. Dengan demikian dapat dinyatakan bahwa semakin tinggi konsentrasi maka semakin besar pula daya atau zona hambat yang terbentuk
PEMBERDAYAAN MASYARAKAT DALAM SWAMEDIKASI PENYAKIT RADANG SENDI DI DESA MENDALO INDAH KECAMATAN JAMBI LUAR KOTA
No full textPenyakit radang sendi atau sering dikenal dengan istilah rematik merupakan penyakit yang banyak menyerang masyarakat pada usia 25-74 tahun dengan prevalensi dan keparahan yang meningkat dengan usia. Terapi pada penyakit ini bukan hanya dengan pemberian obat tapi juga ditunjang oleh terapi non obat. Pemanfaatan tanaman yang bekhasiat sebagai obat tradisional juga dapat menunjang terapi ini, mengingat pengobatan rematik membutuhkan waktu yang sangat lama, bahkan selama hidup. Hal ini dapat menimbulkan resiko efek samping obat yang dikonsumsi. Dengan demikian diperlukan berbagai terapi non obat serta pengobatan alternatif untuk menunjang penatalaksanaan dari penyakit ini. Program ini akan dilaksanakan di Desa Mendalo Indah, RT 02 Kec. Jambi Luar Kota dengan tujuan memberikan pengetahuan dan keterampilan kepada masyarakat dalam mengatasi penyakit radang sendi (rematik) baik dengan terapi menggunakan obat, obat alternatif maupun dengan terapi non obat seperti diet makanan dan melakukan latihan fisik tertentu agar ketidakberdayaan (disability) yang diakibatkan penyakit ini dapat diatasi sehingga kualitas hidup masyarakat menjadi meningkat dengan mengetahui cara pengobatan yang efektif. Hasil evaluasi pelaksanaan pengabdian yang diikuti oleh warga RT 02, desa Mendalo Indah adalah warga memahami tentang penyakit radang sendi dan bagaimana penatalaksanaannya baik secara farmakogis maupun non farmakologis sehingga mampu melakukan swamedikasi terhadap penyakit itu sendiri untuk menciptakan masyarakat yang peduli dengan kesehatan guna mewujudkan Indonesia sehat. Disamping itu juga diperoleh gambaran insidensi penyakit radang sendi khususnya asam urat pada masyarakat di desa Mendalo Indah
EFFECTIVENESS OF ANTIOXIDANTS AS BROWNING INHIBITORS AGAINST SHOOT Cyrtostachys renda AS CALLUS CULTURE EXPLANT
No full textCyrtostachys renda has various pharmacological activities. C. renda roots contain alkaloids and flavonoids as secondary metabolites' main components responsible for anti-inflammatory and anticancer activities. To produce secondary metabolites from C. renda, tissue culture can be used through callus and cell suspension culture techniques. Browning is one of the main challenges in plant tissue culture, which inhibits the success of callus formation, especially in plants with a high content of phenolic compounds such as C. renda. This study tested the effect of soaking in polyvinylpyrrolidone (PVP) and ascorbic acid (AA) in inhibiting the browning process in C. renda explants. Explant colour change testing was done in vitro by soaking the explants in five concentrations of PVP and AA (50, 100, 150, 200 and 250 ppm). Changes in explant colour were then analyzed using the Royal Horticultural Society (RHS) colour chart. The results showed that the PVP antioxidant with a concentration of 200 ppm could suppress explant browning until the fourth day. In comparison, explants treated with AA antioxidants with a concentration of 200 ppm showed browning symptoms on the second day. It can be concluded that antioxidants can reduce the browning level in C. renda shoot explants, and the antioxidant PVP can reduce the browning level better than AA. The mechanism of PVP as an anti-browning agent through inhibition of phenolic oxidation. This study's novelty is obtaining the type of anti-browning PVP with an optimum concentration of 200 ppm to inhibit the browning of C. renda shoot explants
DIVERSIFIKASI WORTEL MENJADI PERMENJELLY SEBAGAI UPAYA MENGATASI ANAK SULIT MENGKONSUMSI SAYUR
No full textLatar Belakang: Wortel merupakan salah satu sayuran yang terkenal dengan kandungan Beta Karoten (Vitamin A) yang sangat tinggi, didalamnya juga terdapat vitamin B dan E. Beta Karoten yang terdapat pada wortel memiliki 2 fungsi yaitu untuk menjaga kesehatan mata dan sebagai antioksidan berdasarkan hasil penelitian jumlah beta karoten yang terdapat pada wortel mentah sebesar 34,94%. Tanaman Wortel merupakan tanaman yang sudah tidak asing lagi dan sangat sering dikonsumsi oleh masyarakat. Masyarakat sering memanfaatkan dalam bentuk sayuran, bahan masakan, dan jus. Tujuan: Untuk meningkatkan minat anak-anak untuk mengkonsumsi sayur. Selain itu produk inovasi ini juga diharapkan dapat menjadi ilmu bagi masyarakat untuk memanfaatkan berbagai macam sayuran menjadi produk yang memiliki nilai jual. Sehingga nilai jual wortel dipasaran menjadi meningkat serta dapat membantu meningkatkan kesejahteraan para petani wortel. Metode Pelaksanaan: kegiatan dilakukan melalui kegiatan penyuluhan dan pelatihan kepada masyarakat. Hasil : Kegiatan yang telah dicapai dari kegiatan pengabdian masyarakat ini adalah bertambahnya pengetahuan masyarakat tentang diversifikasi wartel menjadi produk permen jelly yang disukai seluruh kalangan baik anak-anak maupun orang dewasa
Green Synthesis nanopartikel Tembaga Menggunakan Reduktor Alami Ekstrak Daun Gambir (Uncaria Roxb Extract) dan aktivitas antibakterinya
No full textSUMMARY
Nanotechnology and nanomaterials have become a focus of increasing interest to a number of researchers in recent years, because nanometer-sized materials have a number of chemical and physical properties that are superior to bulk materials. The main thing that makes nanoparticles different from similar materials in their large size is the surface properties that can be modified in terms of optical, magnetic, electronic and mechanical properties as well as their small size with a greater ratio between surface area and volume.
Many researches related to the synthesis of nanoparticles have been carried out. However, there are still some weaknesses that are found among them are the high production costs and the use of chemicals that are harmful to users and the environment. Therefore, it is necessary to develop a synthesis method that is more environmentally friendly by using natural reducing agents that are safe (non-toxic), cheap and easy to obtain. In addition, this method requires a relatively short contact time compared to other methods.
In this study, copper nanoparticles were synthesized using natural reducing agents from gambier leaf extract with variations in copper precursor anions and reaction times and antibacterial activity. Gambier leaf extract acts as a reducing agent and capping agent. The reason for choosing gambier leaves is that the availability is quite abundant and easy to obtain, considering that West Sumatra is the largest gambier producing area (80%) in Indonesia. The choice of copper as a precursor metal is due to its potential applications in various fields, including as a material for conductors, catalysts, detectors, optical sensors, and antimicrobial agents. In addition, the synthesis process is very easy, the costs used are relatively cheap and affordable and the availability is sufficient. The development of the copper metal synthesis method to produce nanoparticles is directed to produce NpCu in the size of 1-100 nm.
From previous literature studies, it has not been found that the use of gambier leaf extract as a copper metal reducer in the synthesis of NpCu and its characteristics and application as antibacterial. Starting from the literature search, the authors are interested in further researching the method of copper nanoparticle synthesis with gambier leaf extract and its antibacterial activity on gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria.
In this study, three types of precursors were used, CuSO4, Cu(NO3)2 and CuCl2. Based on the research results obtained information on the potential of gambier leaf extract in reducing copper to NpCu. The formation of NpCu colloids is visually indicated by a color change from light brown to dark brown. The results of measurements with a UV-Vis spectrophotometer formed an absorption peak at a wavelength of 405-427 nm which is a specific wavelength of the formation of NpCu based on the Surface Plasmon Resonance (SPR) phenomenon. The results of the X-Ray Diffraction (XRD) analysis showed the results of NpCu with a Face Centered Cubic (FCC) crystal structure through the formation of 3 peaks in the 43.28 ° (111), 50.40 ° (200) and 74.81 ° (220) areas. The results of FTIR analysis showed that there was a vibration of Cu-O bonds which assumed interaction between Cu ions and polyphenol compounds at wave numbers 623 cm-1 for CuSO4, 615 cm-1 for Cu(NO3) 2 and 610 cm-1 for CuCl2 precursors. Transmission Electron Microscope (TEM) shows the synthesized NpCu is spherical. The results obtained by using the CuSO4 NpCu precursor formed tended to be more evenly distributed than the Cu(NO3)2 and CuCl2 precursors with particle size diameters of 15 nm, 28 nm and 25 nm, respectively. The size and morphology of these copper nanoparticles are influenced by the type of precursor anions used. The higher the polarity, the solubility and the anion charge, the more stable and the smaller the NpCu particle size. Based on the characterization results, it can be concluded that with better NpCu produced from CuSO4 precursors compared to the other 2 precursors Cu (NO3)2 and CuCl2. The precursors for CuSO4, Cu(NO3)2 and CuCl2 were 13.8 mm, 12.6 mm and 11.9 mm respectively and gram negative Escherichia coli with inhibition zone areas of 11.8 mm, 10.8 mm and 10.5 mm. This shows that NpCu has the potential as a strong category antibacterial agent. From the research conducted, the author has succeeded in discovering the potential of gambier leaf extract as a reducing agent and capping agent in synthesis. Copper nanoparticles are powdered with a size of 15 - 28 nm, a round and stable shape of the three precursors (CuSO4, Cu(NO3)2 and CuCl2). The resulting NpCu has been shown to act as a strong antibacterial agent with the formation of an inhibition zone of 11-13 mm. Advantage
Acute Toxicity Test of Ekor Naga (Rhaphidophora pinnata (L.f) Schott) Leaf Extract in Mice and Kidney Histological Examination
No full textEkor Naga (Rhaphidophora pinnata (L.f) Schott) is a traditional plant used by the community. Previous studies have shown that this plant has pharmacological effects, including as anthelmintics, antioxidant, and anti-inflammatory agent as well as promotes wound healing. Thus, it is necessary to do a toxicity test. The study aimed to determine the effect of the extract on histopathology of the kidney through a toxicity test. The research used an experimental design. The test animals were divided into 5 groups, each of which consisted of 5 test animals: negative control (0.5% Na. CMC), Treatment 1 (Extract of ekor naga leaves at a dose of 200 mg/Kg BW), Treatment 2 (Extract of ekor naga leaves at a dose of 600 mg/Kg BW), Treatment 3 (Extract of ekor naga leaves at a dose of 1800 mg/Kg BW), and Treatment 4 (Extract of Ekor Naga Leaves at a dose of 5400 mg/Kg BW). The parameters observed were the Letal Dose (LD50) and the histophatology of the kidney. Data were analyzed using One Way ANOVA test, followed with Duncan’s test. The results showed no significant difference in the weight of the kidney (p>0.05), and the histophatology of the kidney after 14 days of acute administration of ekor naga leaf extract in all the treatment was normal. The administration of ekor naga leaf extract at therapeutic doses and larger doses of single administration did not have a bad effect on the histology of the kidney.Keywords: Ekor Naga Leaves, Kidney, Histopathology, Acute Toxicity
Evaluation of Antibacterial Activity Test For Active Extract Of Red Palm Fruit (Cyrtostachys renda Blume) against Staphylococcus aureus and Escherichia coli
No full textThe two bacterial species most commonly associated with human illnesses are Staphylococcus aureus and Escherichia coli. The red palm (Cyrtostachys renda Blume) shows promise as a natural source of antibacterial agents due to its high concentration of metabolite compounds, which include phenolics, flavonoids, saponins, tannins, and terpenoids. Antibacterial activity was evaluated using the pitting diffusion method at concentrations of 92%, 46%, and 23%. The results indicated that the ethyl acetate extract exhibited the strongest antibacterial activity, with an inhibition zone measuring 26.93 mm against Eschericia coli and 20.50 mm against Staphylococcus aureus at the highest dose. Additionally, the aqueous extract demonstrated significant inhibition, while the n-hexane and dichloromethane extracts showed no antibacterial activity. The validity of the method was confirmed by using chloramphenicol as the positive control and 10% DMSO as the negative control. These findings suggest that red palm fruit has potential as a natural antibacterial agent derived from local Indonesian plants
Evaluation of Antibacterial Activity for Non-Polar and Polar Fractions of Red Palm Fruit (Cyrtostachys renda Blume) against the Staphylococcus aureus and Escherichia coli
No full textAn unfavorable climate may contribute to the spread of infections. Infectious diseases can be caused by bacteria, fungi, viruses, or parasites. Phytochemical screening of fractionates ethyl acetate extract from red palm fruit (Cyrtostachys renda B.) has identified several secondary metabolites with antibacterial properties. These components include phenolics, tannins, and saponins. Secondary metabolites were fractionated using Vacuum Liquid Chromatography (VLC) and Column Chromatography (CC) techniques, resulting in both polar and non-polar fractions. The polar fraction of red palm fruit, extracted with methanol, shows antibacterial activity exclusively against gram-negative bacteria. The most significant inhibition zone is observed at a concentration of 92%. In contrast, the non-polar fraction, obtained using ethyl acetate, demonstrates antibacterial activity against both gram-positive and gram-negative bacteria. The concentration used affects the size of the inhibitory zone formed
