1,720,969 research outputs found
ABID
No full textIn order to automate the spectral comparison for larger libraries of antibodies, we developed the online software ABID 2.0. This open-source software determines the number of matching peptides in the fingerprint spectra. We propose that publications and other documents critically relying on monoclonal antibodies with unknown amino acid sequences should include at least one antibody fingerprint. By fingerprinting an antibody in question, its identity can be confirmed by comparison with a library spectrum at any time and context
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Pflanzenviren als chemische Plattform für die Nanotechnologie
Get PDFDie Proteinhülle von Pflanzenviren besteht aus Protein-Untereinheiten, die das Genom des Virus verkapseln und transportieren. Der zugrunde liegende self-assembly-Mechanismus kann für nanotechnologische Anwendungen genutzt werden, indem Fremdmoleküle in den Viren verkapselt werden. Diese sogenannten Virus-ähnlichen Partikel (VLP) können zum Wirkstofftransport in der Krebstherapie, für Gentherapie oder als Kontrastmittel bei bildgebenden Verfahren verwendet werden. Auch die Oberfläche der Proteinhülle kann chemisch oder molekularbiologisch funktionalisiert werden. Ein großer Vorteil von Pflanzenviren ist, dass sie keine Gefahr für den Menschen darstellen und einfach in natürlichen Wirtspflanzen hergestellt werden können.
In dieser Arbeit wird am Beispiel des Cowpea Chlorotic Mottle Virus (CCMV) gezeigt, wie diese Nanomaterialien effizient hergestellt und nutzbar gemacht werden können.
Im ersten Kapitel wurde eine Affinitätsextraktion basierend auf einem neuen Peptid-Aptamer entwickelt, um das CCMV selektiv aus dem Extrakt der infizierten Pflanze zu isolieren. Das Reinigungsprotokoll wurde validiert und eine finale Reinheit von 98,4% mittels Umkehrphasen-HPLC bestimmt.
Im zweiten Kapitel wurden CCMV-VLPs durch rekombinante Expression und anschließende in vitro Assemblierung der Kapsidproteine hergestellt. Die Monodispersität der VLPs wurde durch Transmissionselektronenmikroskopie (TEM) bestätigt. Zusätzlich wurde eine Mutante der CCMV-VLPs hergestellt, die physiologischen Bedingungen standhält und für den Einsatz in vivo geeignet ist.
Im dritten Kapitel wurden CCMV und dessen VLPs für nanotechnologische Anwendungen genutzt. Gold-Nanopartikel wurden in CCMV-VLPs verkapselt und im TEM sichtbar gemacht. Das CCMV-Kapsid wurde mit einem Modellpeptid funktionalisiert, um das Potenzial dieses Nanomaterials als chemische Plattform zu demonstrieren.The protein shell of plant viruses consists of protein subunits that encapsulate and transport the viral genome. The underlying self-assembly mechanism can be utilized for nanotechnological applications by encapsulating foreign molecules within the viruses. These so-called virus-like particles (VLPs) can be used for drug delivery in cancer therapy, gene therapy, or as contrast agents in imaging techniques. Additionally, the surface of the protein shell can be functionalized chemically or via molecular biology techniques. A significant advantage of plant viruses is that they pose no threat to humans and can be easily produced in natural host plants.
This work demonstrates how these nanomaterials can be efficiently produced and utilized using the example of Cowpea Chlorotic Mottle Virus (CCMV).
In the first chapter, an affinity extraction based on a novel peptide aptamer was developed to selectively isolate CCMV from the extract of the infected plant. The purification protocol was validated, achieving a final purity of 98.4% using reverse-phase HPLC.
In the second chapter, CCMV-VLPs were produced through recombinant expression and subsequent in vitro assembly of the capsid proteins. The monodispersity of the VLPs was confirmed by transmission electron microscopy (TEM). Additionally, a mutant of the CCMV-VLPs was produced that withstands physiological conditions and is suitable for in vivo applications.
In the third chapter, CCMV and its VLPs were used for nanotechnological applications. Gold nanoparticles were encapsulated within CCMV-VLPs and visualized using TEM. The CCMV capsid was also functionalized with a model peptide to demonstrate the potential of this nanomaterial as a chemical platform
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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