1,034 research outputs found
Kosciusko [music] /
No full textFor voice and piano.; Cover title.; "Introduced & sung by Miss Nella Webb."; Cover carries portraits of Nella Webb (by Rudolph Buchner), Charles Vaude and Moritz Lutzen.; Words printed as text on p. [4].; "During Moritz Lutzen's visit to Australia he offered a prize for the best lyric, by an Australian author to be set to music by himself. The prize was awarded to Charles Vaude, for his lyric 'Kosciusko,' and Miss Nella Webb produced this song with instantaneous success."--P. [4].; Also available online http://nla.gov.au/nla.mus-an8393500; 1913, by Victor J. Draper, Sydney.; NLA's NL copy from the collection of Keith Watson. ANL
Letter containing inquiry regarding the ethnic identity of the descendents of Georg Moritz Oppenheim.
No full textLetter from Wilhelm Gehlig to Rabbi Dr. Freudenthal in Nuremberg with a genealogical question regarding Georg Moritz Oppenheim. Of particular interest to the author is to determine whether Oppenheim's descendents are "rein jüdischen Blutes (=of pure Jewish blood)."Robert Singermandigitize
Conventional and circular economy compliant modification strategies for recycled polypropylene
No full textAuthor Moritz MagerMasterarbeit Universität Linz 2021Arbeit gesperr
Conventional and circular economy compliant modification strategies for recycled polypropylene
No full textAuthor Moritz MagerMasterarbeit Universität Linz 2021Arbeit gesperr
Identification of Toxoplasma gondii effector proteins by pooled CRISPR-Cas9 knockout screening
No full textToxoplasma gondii is an intracellular eukaryotic pathogen of global clinical significance. Secretion of effector proteins by T. gondii into a host cell is critical for infection. At least two hundred putative effector proteins are secreted into the host cell, of which the vast majority are uncharacterised. In this work, I used CRISPR-Cas9 knockout screening in T. gondii to identify effector proteins involved in parasite evasion of host immune responses.
From a screen of T. gondii mutants in a mouse model of infection, I identified genes that confer parasite fitness in vivo. I found that the first-identified rhoptry protein of T. gondii, ROP1, is necessary for parasite virulence and is important for evasion of the cell-autonomous, interferon-gamma-stimulated immune response in both murine and human macrophages.
Then, I adapted CRISPR-Cas9 tools in T. gondii to enable pooled knockout screening with a single-cell, dual host-parasite transcriptome readout. This method, which I term dual perturb-seq, enables identification of parasite effectors that modulate host cell transcription in a high-throughput and unbiased manner. Using dual perturb-seq and additional cellular assays, I show that the dense granule protein GRA59 contributes to export of other dense granule effectors from the parasitophorous vacuole to the host cell. I also identify a rhoptry protein, newly named TgSOS1, that is required for sustained parasite-induced STAT6 signalling in the host cell and M2 polarisation of infected macrophages.
Together, these genetic screens and the characterisation of newly identified effector proteins advance the understanding of how T. gondii manipulates the host cell and evades host immune responses.Open Acces
Functional characterisation of the FIKK kinase family of Plasmodium falciparum
Get PDFKey to P. falciparum virulence is its capacity to remodel the host erythrocyte. Infected erythrocytes become rigid and cytoadhere to the vascular endothelium leading to the disease symptoms and preventing their filtration by the spleen. Unlike other human- infecting Plasmodium species, P. falciparum exports a family of 18 FIKK kinases into the host cell.
Here, a conditional knockout strategy based on the DiCre/LoxPint technology was used to study 4 FIKK kinases (FIKK4.1, FIKK7.1, FIKK10.1 and FIKK11) and identify their potential targets by quantitative phosphoproteome analysis. The deletion of FIKK4.1 led to a significant reduction in the phosphorylation of host cytoskeletal proteins and parasite proteins involved in remodelling. The characterisation of FIKK4.1 KO parasites confirmed its role both in the rigidification of the infected erythrocytes and in the trafficking of the adherence-mediating virulence factor PfEMP1 to the host cell surface.
Additionally, recombinant versions of several FIKK kinase domains were used to identify potential pan-FIKK inhibitors. When tested in vitro, these compounds showed activity on both P. falciparum and P. knowlesi, raising concerns regarding their specificity. A whole genome sequencing on drug-resistant parasites did not allow to identify additional targets. Moreover, it was shown that the compounds were not active on the FIKK kinases in culture due to the high intra-erythrocytic ATP concentration.
Using the recombinant FIKK kinase domains it was also shown that FIKK kinases possess distinct substrate specificity. Whereas most of them conserved the ancestral basophilicity, some evolved to phosphorylate preferentially acidic motifs. Strikingly, FIKK13 was found to be a tyrosine kinase, a feature supposed to be absent in Plasmodium. Finally, by studying the FIKK kinases from another Plasmodium species closely related to P. falciparum, it was shown that FIKK kinases substrate specificity is conserved across species of the Laverania clade.Open Acces
Hayo Haya Maaseh [= Once upon a time]
No full textAnthology of East European Jewish folklore, with introduction and sources. The anthology includes: songs, tales, traditions, customs, jokes, proverbs, riddles. Authored by Chaim Ben Zion Elon-Baranik (born 1901). Published immediately after the Second World War. Illustrations by Moritz Oppenheimer, L. Pilichowski, Yosef Budko, E.M. Lilien (his signature in print). Most of the illustrations are printed on separated chrome paper, on one side of the page. Folklore publishing, Tel Aviv. HaIvri press, Jerusalem. 22 em. [1],303, [3] pages. Excellent condition. Chipped top of spine. . '... Olb price140-160This is a hardbound book (hard cover)Language note: HebrewChaim Ben Zion Elon-Barani
Functional characterisation of cyclase-associated protein (CAP) in Toxoplasma gondii
Get PDFToxoplasma gondii progression through the lytic cycle depends on a divergent actin and an array of associated unconventional myosin motors. These acto-myosin systems power mechanical processes, such as active invasion and egress of host cells, cell division and organellar trafficking. How actin-associated proteins regulate the organisation and turnover of the actin filaments to support these diverse processes is poorly understood. Cyclase-associated protein (CAP) is an actin-binding protein conserved across eukaryotes. We show that Toxoplasma CAP has a unique extension that is not present in most other Apicomplexa, giving rise to two isoforms with distinct subcellular localisations: one is localised at the parasite apex while the other is cytosolic. Here we investigated the role of CAP in Toxoplasma biology using a new generation of RH ∆ku80∆hxgprt DiCre parasites where loss of DiCre activity is prevented. Conditional knockout of CAP led to significant defects in motility, invasion, active egress, dense granule trafficking, daughter cell orientation, juxtanuclear accumulation of actin and cell-cell communication but only modest defects in synchronicity of division and no defect in replication of the apicoplast. Despite displaying phenotypes closely resembling the actin knockout, CAP is dispensable for in vitro culture. Strikingly, CAP knockout in the type I RH parasite strain does not affect in vivo virulence but results in complete attenuation of the type II Pru strain. 3D electron microscopy reveals that loss of CAP results in a defect in formation of a normal central residual body, but parasites remain connected within the vacuole. This dissociates synchronicity of division and parasite rosetting and reveals that establishment and maintenance of the residual body may be more complex than previously thought. These results highlight the different spatial requirements for F-actin dynamics in Toxoplasma that depend, in part, on CAP function.Open Acces
Identification and characterisation of Toxoplasma gondii effectors mediating parasite virulence in human cells
No full textToxoplasma gondii is an intracellular eukaryotic parasite that chronically infects
approximately one third of the global human population. To establish an infection,
Toxoplasma must initially avoid clearance by disarming the human immune system.
To do this, Toxoplasma secretes over two hundred effector proteins, which
collectively reshape the intracellular environment and resist innate immune
responses. Previous efforts to characterise Toxoplasma secreted effectors largely
centered on their role in the murine host. However, innate immune control of
Toxoplasma has significantly diverged between humans and mice, therefore
effector functions are not always conserved between the two hosts. Relatively
little is known about which secreted effectors are required for Toxoplasma to
evade the human immune response.
In this work I used targeted CRISPR screening of Toxoplasma secreted effectors to
identify which are required for survival in interferon-gamma-activated human cells.
CRISPR screens were carried out using two Toxoplasma strains that differ in
virulence in mice. From these screens I found that a complex of secreted dense
granule proteins (GRA57, GRA70, and GRA71) resists interferon-gamma-induced
parasite clearance in human fibroblasts. This complex was conversely dispensable
for parasite survival in mouse fibroblasts, suggesting it may have species-specific
functions. Additionally, I found that multiple components of the Toxoplasma
effector export machinery are required for immune resistance in human and mouse
fibroblasts. I next investigated the function of the GRA57, GRA70, and GRA71
complex, and ruled out roles in effector export, transcriptional modulation,
tryptophan acquisition or formation of the parasite vacuole. I instead found that
GRA57, GRA70, and GRA71 knockout parasite vacuoles are less ubiquitinated by
the host cell, but this is uncoupled from their function in immune resistance.
Together these results advance our previously limited understanding of which
Toxoplasma effector proteins are required for the parasite to establish human
infections.Open Acces
Karl Philipp Moritz\'s essays: language, arts, philosophy (selection, introduction, translation and notes)
No full textApós mais de duzentos anos, o interesse pela obra de Karl Philipp Moritz (15 de setembro de 1756 26 de junho de 1793) só tem aumentado. Diferentes autores como Herman Hesse e Walter Benjamin, e, mais recentemente, Hans Joachin Schrimpf, Tzvetan Todorov, Peter Szondi, Arno Schmidt e Peter Handke têm escrito ressaltando a importância e a fecundidade desse autor. Moritz pode ser considerado um dos autores inaugurais do romantismo alemão. Este mestrado em filosofia, área de estética, pretende, por meio de seleção, tradução e introdução dos textos de Karl Philipp Moritz, contribuir para a valorização dessa importante obra em nossa cultura. Os textos selecionados são de teoria da linguagem, estética e filosofia.After over two hundred years, the concern for the works of Karl Philipp Moritz (September 15th 1756 June 26th 1793) has increased steadily. Different authors such as Herman Hesse and Walter Benjamin and more recently Hans Joachin Schrimpf, Tzvetan Todorov, Peter Szondi, Arno Schmidt and Peter Handke have written on the relevance and fecundity of this author. Moritz can be said to be one of the inaugural authors of German Romanticism. This Masters in Philosophy, in the Aesthetics field, intends, by means of selection, translation and introduction of Karl Philipp Moritz texts, to contribute to the appreciation of this important work in our culture. The selected texts belong to the fields of Language Theory, Aesthetics and Philosophy
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