1,354,655 research outputs found
Precs Participant: Isako Di Tomassi
No full textIsako Di Tomassi, a participant in the Phenotypic Plasticity Research Experience for Community College Students, discusses his experience and assigned project
Il programma di ricerca di ricerca e sviluppo CEE sulle applicazioni dell’informatica e delle telecomunicazioni all’assistenza sanitaria
No full text
The central proline rich region of POB1/REPS2 plays a regulatory role in epidermal growth factor receptor endocytosis by binding to 14-3-3 and SH3 domain-containing proteins
No full textBackground. The human POB1/REPS2 (Partner of RalBP1) protein is highly conserved in mammals where it has been suggested to function as a molecular scaffold recruiting proteins involved in vesicular traffic and linking them to the actin cytoskeleton remodeling machinery. More recently POB1/REPS2 was found highly expressed in androgen-dependent prostate cancer cell lines, while one of its isoforms (isoform 2) is down regulated during prostate cancer progression. Results. In this report we characterize the central proline rich domain of POB1/REPS2 and we describe for the first time its functional role in receptor endocytosis. We show that the ectopic expression of this domain has a dominant negative effect on the endocytosis of activated epidermal growth factor receptor (EGFR) while leaving transferrin receptor endocytosis unaffected. By a combination of different approaches (phage display, bioinformatics predictions, peptide arrays, mutagenic analysis, in vivo co-immunoprecipitation), we have identified two closely spaced binding motifs for 14-3-3 and for the SH3 of the proteins Amphiphysin II and Grb2. Differently from wild type, proline rich domains that are altered in these motifs do not inhibit EGFR endocytosis, suggesting that these binding motifs play a functional role in this process. Conclusion. Our findings are relevant to the characterization of the molecular mechanism underlying the involvement of POB1/REPS2, SH3 and 14-3-3 proteins in receptor endocytosis, suggesting that 14-3-3 could work by bridging the EGF receptor and the scaffold protein POB1/REPS2. © 2008 Tomassi et al; licensee BioMed Central Ltd
Entrevista a Horacio González
No full textFil: Manfredi, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). UNER; Argentina.Fil: De Tomassi, Facundo. Universidad Nacional de Rosario; Argentina
ANCHE L’ARTE PARLA DI SOSTENIBILITÀ
No full textL’umanità è su un sentiero di non sostenibilità. È un concetto che viene ribadito quotidianamente da media, esperti del settore e da svariate campagne di sensibilizzazione, più o meno efficaci. Si tratta di un argomento delicato che interessa trasversalmente tutta la società. In questo contesto, nemmeno l’arte può esimersi dall’interrogarsi su questo problema contemporaneo, e così alcuni artisti, sensibili alle tematiche ambientali, indirizzano la loro sensibilità a questa materia. La manifestazione del rapporto di affinità tra arte e sostenibilità si è concretizzata con l’esposizione di 17 dipinti, ciascuno dei quali collegato ad ognuno dei 17 SDGs (Sustainable Development Goals – Obiettivi per lo Sviluppo Sostenibile dell’ONU), rivisitati in chiave artistica mantenendone la forma, il colore e i simboli. Il presente contributo si pone l’obiettivo di descrivere tali opere con il fine di sensibilizzare la comunità alla comprensione dell’Agenda 2030
Psychiatric disorders
No full textThis chapter summarizes the most significant gender influences on mental health in terms of illness incidence and prevalence, clinical presentation, course, and response to treatment. Several mental disorders including major depression, bipolar disorder, anxiety disorders, schizophrenia, and eating disorders are considered in different sections. Depression is twice more frequent in women than in men. Moreover, men and women show differences regarding presentation, course, treatment response, and outcome. Women affected by depression show higher recurrence and atypical features; they have generally an earlier onset, more severe, longer, and recurrent depressive episodes, and a lower quality of life than men do. Women are also more likely to have a comorbid anxiety, eating or somatoform disorder, and more frequently than men, they attempt suicide (although lethal suicide is more probable to happen in men). Psychopharmacological treatment of depression also might present significant gender dissimilarities; still, there is no clear consensus on whether there are gender-related differences in antidepressant efficacy. There is a significant gender difference in terms of lifetime prevalence of bipolar disorder type II, with more affected women, while both genders show a similar prevalence of bipolar disorder type I. Women usually have an older age of onset and they typically manifest a depressive polarity at the onset and a predominance of depression phases during lifetime. Women are also more likely to undergo mixed and seasonal episodes and have an increased risk of developing rapid cycling mood disturbances. Bipolar disorder in men is characterized by manic onset, recurrence of manic phases, and by lower treatment adherence. Comorbidity of psychiatric (eating and anxiety disorders) and medical (thyroid disease, migraine, obesity) conditions are more common in women, while substance use disorder is more common in men. There is no evidence that women and men suffering from bipolar disorder differ significantly in treatment response to mood stabilizers. Schizophrenia also has significant gender differences: affected males, normally younger at the onset than females, present more severe negative symptoms, worse cognitive impairment, more frequent hospitalizations, and are more likely to commit acts of severe violence. In detail, incidence rate of early onset is higher in males than females, while at older onset women predominate. Until the mid-30s, rates are estimated to be approximately 1.5–2 times greater in males than females. Later, rates decrease for both sexes, with a narrowing sex ratio, until the mid-40s when there is a minor secondary peak for women. Male patients are likely to have more cognitive impairment and poorer premorbid functioning, more negative symptoms, and more severe deterioration over time. Female patients experienced more severe positive symptoms (hallucinations and persecutory delusions) and commit a greater number of suicide attempts. Women also show a considerably less severe course of the illness: they show a better social functioning and have fewer hospitalizations with shorter inpatient stays. Gender differences have also been well recognized in the response to antipsychotic treatment, with women being better responders than men are. Eating disorders in the past were considered as almost exclusively female disorders (F:M = 20:1), but that is changing rapidly. One million men have been shown to suffer from eating disorders in the USA. Males accounted for roughly 10.0–25.0% of eating disorder patients, with the number of men struggling from bulimia nervosa being more than those who struggle with anorexia. Research and knowledge on the topic are expanding rapidly, and recent literature elucidates gender-specific issues in terms of age of onset, weight history and compensatory exercise behavior, frequency of abuse record, and substance use rates. A later age of onset, premorbid obesity, and over-exercise are more likely in men. Around 30% of subjects suffering from an eating disorder were victims of sexual abuse (1:3 in women vs. 1:7 in men) and a substance use disorder is generally more frequent in subjects with eating disorder (particularly the use of steroids and growth hormones in affected men) in comparison to the general population
La probabilità, ancella e regina dell’analisi matematica. (Prendere sul serio Paul Lockhart, a ogni livello dell’insegnamento.)
No full textPolycomb Repressive Complex 2 Modulation through the Development of EZH2-EED Interaction Inhibitors and EED Binders
No full textEpigenetics is nowadays a well-accepted area of research. In the last years, tremendous progress was made regarding molecules targeting EZH2, directly or indirectly. Recently tazemetostat hit the market after FDA-approval for the treatment of lymphoma. However, the impairment of EZH2 activity by orthosteric intervention has proven to be effective only in a limited subset of cancers. Considering the multiproteic nature of the PRC2 complex and the marked dependence of EZH2 functions on the other core subunits such as EED, in recent years, a new targeting approach ascended to prominence. The possibility to cripple the function of the PRC2 complex by interfering with its multimeric integrity fueled the interest in developing EZH2-EED protein-protein interaction and EED inhibitors as indirect modulators of PRC2-dependent methyltransferase activity. In this Perspective, we aim to summarize the latest findings regarding the development and the biological activity of these emerging classes of PRC2 modulators from a medicinal chemist's viewpoint
Auxin, photoperiod and putrescine affect flower neoformation in normal and rolB-transformed tobacco thin cell layers
No full textAuxin, photoperiod and putrescine are factors involved in the control of the reproductive phase of the flowering process in planta. The aim of this study was to investigate whether auxin is able, in the absence of other exogenous hormones, to stimulate flower neoformation in Nicotiana tabacum L, thin cell layers excised from flowering plants, whether an interaction exists between the hormone and the length of the light treatment per day, and whether exogenous putrescine interferes with auxin and light in the realization of the flowering programme. Since tissues transformed with rolB gene show an increased binding capacity of auxin, the response of rolB-explants was compared with that of the wild type. Thin cell layers from floral branches were cultured in the presence of a wide range (0-10 mu M) of indoleacetic acid alone, under various phototreatments (continuous darkness, 8 h light/day, and 16 h light/day). Floral, vegetative and rooting responses were favoured in the transgenic explants. Flower neoformation was stimulated by long days and by exogenous auxin (1 mu M). In rolB-thin cell layers flowering also occurred in the most unfavourable culture conditions (i.e. continuous darkness combined with either hormone-free medium or 10 mu M auxin). Long days and 1 mu M auxin also favoured caulogenesis, though to a lesser extent than flower neoformation. Rhizogenesis occurred in rolB-explants only and was induced by exogenous auxin and favoured by continuous darkness, In both wild type and rolB-explants, exogenous putrescine (1 mM) reduced flower neoformation in the presence of 1 mu M auxin and long days. (C) Elsevier, Paris
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