1,721,222 research outputs found
What is new in melanoma research: genetics and epidemiology of melanoma in 2003? Review of a workshop held in Milan in May 2003
No full textDermatoscopic and histopathological diagnosis of primary and metastatic melanoma: Report of a workshop at the Third Research Meeting on Melanoma, Milan, Italy, May 2003
No full textThe clinical and histopathological session of the Third Research Meeting on Melanoma, Milan, Italy, held in May 2003, highlighted several key topics, including clinical, dermatoscopic and computer-aided diagnosis and the use of immunohistochemical, immunological and biochemical approaches to diagnosis and monitoring. This report is a brief summary of the main themes covered. (C) 2004 Lippincott Williams Wilkins
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Heterologous expression in B.subtilis. II: in vitro removal of the attenuator sequence of the E.coli his operon allows expression in B.subtilis of the cloned hisG gene.
No full textThe promoter-proximal region of the Escherichia coli histidine (his) operon, including the promoter, the attenuator and the hisG gene, as well as the first of the nine structural genes of the his operon, have been cloned in Bacillus subtilis. In this host, the hisG gene could not be expressed because its transcription appeared to be irreversibly terminated at the attenuator (Ferretti et al., 1984). When the attenuator plus various lengths of the two bordering regions were removed, one of the attenuatorless sequences cloned in B. subtilis allowed the progression of transcription and complementation of the corresponding hisA mutation in this Gram-positive host. The deletion removed a 349-bp segment which contained the his attenuator and promoter. In B. subtilis, the productive transcription of the hisG gene started at a site in pAT153 and terminated in pC194. Sequence analysis of the deletion indicates that the E. coli ribosome-binding site of the his operon was used for the translation of the E. coli hisG gene mRNA in B. subtilis cells, which can thus grow in the absence of histidine
Diagnosis of melanoma in the elderly and surgical implications
No full textThe diagnosis of primary melanoma is mainly related to the precocity on which a patient is referred to the specialist, but in elderly patients this may present some peculiar characteristics, one is anatomical, a typical melanoma of the face, the lentigo maligna melanoma and the second is attitudinal, the fact that elderly patients often do not refer a changing cutaneous lesion to a doctor until becoming symptomatic. The therapeutic approach has to be discussed with an anaesthesiologist if the procedure has to be conducted under general anaesthesia or with a cardiologist if under local anaesthesia. Once there are no contraindications medically, a similar oncological approach should be proposed without any reduction in radicality due to the elderly age. © 2004 Elsevier Ltd. All rights reserved
Treatment of metastatic melanoma with electrochemotherapy.
No full textSuperficially metastatic melanoma still represents a challenging situation for oncologists and a distressing condition for patients. In this circumstance, local treatment by electrochemotherapy, that is, the combination of brief permeabilizing electric pulses with a low‐toxicity anticancer drug, can be a valuable treatment option in selected patients who are ineligible/refractory to systemic treatments or require rapid palliation of symptomatic cutaneous disease
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