3,177 research outputs found
Assessment, dissemination and measurement of the impact of the fifth research programme on technical control of nuisances and pollution at the place of work and in the environment of iron and steelworks. Final report. EUR 17584 EN
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Making use of historical case material – the problems of looking back and the implications for service development in relation to research and evaluation activities
No full textThis methodological paper details the process of embarking on a follow-up study of young people with sexual behaviour problems who were known to services in the 1990s and who are now young adults in their twenties or early thirties. In the context of the importance of such follow-up work, the overall aim and objectives of the funded research project are specified and the challenges presented in setting up research partnerships with service sites, including the negotiation of access and ethical approval, are the subject of overview and reflection. The practicalities of beginning the fieldwork which comprised an initial analysis of historical case material held in the research sites are then detailed and the solutions to the problems encountered are explained. The article concludes by identifying the kinds of questions services and researchers need to consider when wanting to engender or enhance a research culture which is facilitative of this kind of outcome research. These relate to the resources necessary to support a research culture, the requirements of data protection and ethical approval processes, obtaining service user consent to participate in future research, secure but accessible storage of records, staff development and researchers’ obligations to minimise disruption to already hard pressed services
Mechanisms of sphingolipid functions during heat stress in "Saccharomyces cerevisiae"
No full textSphingolipids are important membrane constituents in all eukaryotic cells. Ongoing sphingolipid synthesis is required for a variety of cellular processes including response to heat shock. During heat stress, de novo sphingolipid synthesis is upregulated and mutants defective in the biosynthesis of sphingolipids show a significantly greater loss of viability. To dissect the mechanisms of sphingolipid functions during heat stress we made use of the Saccharomyces cerevisiae lcb1-100 mutant. This mutant is conditionally impaired in serine palmitoyltransferase activity, the first committed step in sphingolipid biosynthesis. lcb1-100 cells are highly sensitive to heat shock and die at elevated temperatures. In this study we could show that the increased sensitivity to heat stress in lcb1-100 cells correlated with a lack of induction of the major heat shock proteins at high temperature. This defect could be restored by the overexpression of ubiquitin, a situation that increased turnover of proteins and prevented accumulation of protein aggregates in lcb1-100 cells. These data showed that the essential function of heat shock protein induction is the removal of misfolded or aggregated proteins. Furthermore it suggested that heat stressed cells do not die because of the loss of protein activity due to their denaturation, but because of the inherent toxicity of the denatured and/or aggregated proteins. In addition we tried to explain the lack of induction of heat shock proteins in lcb1-100 cells. We could show that transcription and nuclear export of heat shock protein mRNA was not affected in these cells. Under heat stress conditions, lcb1-100 cells exhibit a strong decrease in protein synthesis and polysome analyses demonstrated a defect in translation initiation. Furthermore we demonstrate that efficient translation under these conditions relies upon the synthesis of sphingoid base. Deletion of the eIF4E binding protein Eap1p partially restores translation initiation and the synthesis of heat shock proteins in lcb-100 cells. Thus, sphingoid bases signal to the cap-dependent translation initiation apparatus to enhance heat shock protein synthesis. In addition, ubiquitin overexpression in the lcb1-100 mutant allowed recovery of translation, but not at the initial phase where heat shock proteins are made. Therefore the recovery process seems to be dependent upon the function of the heat shock proteins that are made during the initial phase of heat stress. Altogether we have uncovered a new function for sphingoid bases and provide an explanation for the sphingoid base synthesis requirement for survival during heat stress
Influence of Carbon in Iron on Characteristics of Surface Modification by EDM in Liquid Nitrogen
No full textMany surface modification technologies have been proposed and carried out practically by CVD, PVD et.al. Carbonized layer has been made using EDM method. In this paper, to make the nitride layer by EDM some new trials were carried out using a titanium electrode in liquid nitrogen. Experiments were carried out on carbon steel (S45C), pure iron and cast iron. TiN can be obtained on EDMed surface. Moreover, TiCN can be found on cast iron and steel (S45C) by XRD investigation. To confirm the fabrication mechanisms of modified layer on the steel, the following experimental factors were investigated by EDS
EXPERIENCIA EDUCATIVA : TALLER DE RECURSOS NATURALES II
No full textEl Taller de Recursos Naturales II (TRN II) se desarrolla durante el 2do cuatrimestre del segundo año de Agronomía. La información obtenida por los alumnos es utilizada en el Taller de Producción Vegetal en el 4to Año. De acuerdo a la estructura y lineamientos del Plan de Estudios, el TRN II integra actividades de las asignaturas dictadas en ese cuatrimestre como: Propiedades Edáficas y Fertilidad, Fisiología Vegetal, Genética Básica y Aplicada y Agrometeorología, además de Ecología y Zoología Agrícola, asignaturas de tercero y cuarto año respectivamente. Tiene como objetivo conducir al alumno hacia una compresión global de los factores que afectan el crecimiento de las plantas con el fin de mantener o aumentar la producción, dentro del marco de una agricultura sustentable. Finaliza con una puesta en común donde se interpretan los resultados en términos ecofisiológicos y de interacción genotipo-ambiente, utilizando información obtenida de las demás asignaturas. Así, otorga a los futuros profesionales la capacidad de analizar los conocimientos adquiridos a campo y en el laboratorio, a la luz de los factores investigados durante el desarrollo del Taller
Industry report for P.T. Eztu Adimore Ltd. to export art-crafted glass to the United States
No full textPlan BThis paper analyzes the potential for P.T. Eztu Adimore Ltd. to export art-crafted glass to the United States. The analysis explored different factors, such as assessing the selected market by analyzing that country for market potential, pricing, distribution, and competition. This paper also provides managers with a list of industry contacts, trade shows, and trade associations for future reference, should a firm decide to visit that countr
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