244 research outputs found
Mycobacterium ulcerans mycolactone interferes with adhesion, migration and proliferation of primary human keratinocytes and HaCaT cell line
No full textThe pathogenicity of Mycobacterium ulcerans (Buruli ulcer) is closely associated with the secretion of exotoxin mycolactone. The cytotoxicity of mycolactone has been linked to its apoptogenic activity. We explored if low mycolactone concentrations, which are not able to induce apoptosis, can influence other essential activities on two primary human keratinocyte populations, keratinocyte stem cells (KSC) and transit amplifying cells (TAC), and on a human keratinocyte line, HaCaT. We demonstrated that 0.01 and 0.1 ng/ml mycolactone A/B are not able to induce apoptosis in primary human keratinocytes, but interfere with KSC wound repair. Moreover, the same toxin concentrations reduce cell proliferation of KSC and TAC and their ability to adhere to type IV collagen. HaCaT cells are more resistant to the toxin; nevertheless, they show a delayed woud repair when treated with 1 and 10 ng/ml mycolactone A/B. Moreover, these sub-apoptotic concentrations affect their ability to proliferate and adhere to collagen IV. Wound healing is a complex mechanism, which occurs "in vivo" as the outcome of many co-ordinated events. Sub-apoptotic mycolactone concentrations can affect essential mechanisms, which are required to achieve wound repair, such as adhesion, migration and proliferation of human keratinocytes
"Suspensa semper et obscura verba": le parole di Tiberio negli "Annales" di Tacito
No full textIl lavoro prende in considerazione alcuni aspetti e temi trasversali riscontrabili nelle occorrenze di "oratio recta" attribuite da Tacito all'imperatore Tiberio nella prima esade degli "Annales". Segnatamente, dopo un'introduzione relativa ai discorsi nell'opera annalistica di Tacito e a cenni sulle questioni principali relative alle fonti, si prendono in esame aspetti relativi alla resa del carattere ambiguo di Tiberio nelle scelte stilistico-sintattiche e si cerca di capire quanto i discorsi diretti di questo imperatore possano arricchire il dibattito sull'interpretazione complessiva che ne fa lo storico
Lichen planus as paradoxical side effect of TNF-α inhibitors treatment in one patient with many psoriasis comorbidities
No full textFulminant ulcerative colitis in a healthy pregnant woman
No full textThis case report concerns a 25-year-old patient with 6-7 bloody stools/d, abdominal pain, tachycardia, and weight loss occurring during the third trimester of pregnancy. Severe ulcerative colitis complicated by toxic megacolon and gravidic sepsis was diagnosed by clinical evaluation, colonoscopy, and rectal biopsy that were performed safely without risk for the mother or baby. The patient underwent a cesarean section at 28+6 wk gestation. The baby was transferred to the neonatal intensive care unit of our hospital and survived without complications. Fulminant colitis was managed conservatively by combined colonoscopic decompression and medical treatment. Although current European guidelines describe toxic megacolon as an indication for emergency surgery for both pregnant and non-pregnant women, thanks to careful monitoring, endoscopic decompression, and intensive medical therapy with nutritional support, we prevented the woman from having to undergo emergency pancolectomy. Our report seems to suggest that conservative management may be a helpful tool in preventing pancolectomy if the patient's condition improves quickly. Otherwise, surgery is mandatory
Data protection, scientific research, and the role of information
No full textRossana Ducato is supported by the Innoviris research grant 2016-BB2B-9. The Author wishes to thank Fabrizio Esposito for the useful comments on an earlier version of this paper. The usual disclaimer about the maternity of the mistakes applies.Peer reviewe
Pigmented basal cell carcinomas in Gorlin syndrome: two cases with different dermatoscopic patterns
No full text
A Mycobacterium ulcerans toxin, mycolactone, induces apoptosis in primary human keratinocytes and in HaCaT cells
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