103 research outputs found
Do passeio na avenida à balada no prolonga: sociabilidade no espaço público: o caso da Avenida Getúlio Vargas, Chapecó (SC)
Get PDFDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas, Programa de Pós-Graduação em Antropologia Social, Florianópolis, 2009Esta pesquisa busca compreender as sociabilidades no espaço público da Avenida Getúlio Vargas no município de Chapecó. É resultado de uma pesquisa etnográfica na rua que teve como foco de análise as práticas que delimitam, fragmentam e criam lugares no espaço público chapecoense. São apresentados diferentes contextos de sociabilidade, diferentes cenários que abrigam desde o passeio como uma forma costumeira de uso do espaço público que aparece na parte central da avenida às baladas na rua criadas a partir de práticas de jovens que se reúnem em espaços determinados, criam cenários e pontos de encontro. Estes lugares são constituídos por dinâmicas específicas de sociabilidade, analisadas, nesta dissertação, em torno das relações do ver e ser visto. As diferentes sociabilidades urbanas identificadas na Avenida Getúlio Vargas ressaltam a importância desta enquanto ícone do espaço público da cidade de Chapecó, sendo o envolvente físico e simbólico dessas diferentes sociabilidades. Dos cenários analisados, o prolonga aparece como espaço paradoxal, envolvido simultaneamente por conotações de transgressividade e permissividade. Relações ambíguas que se organizam em termos de coexistência, de simultaneidade e complementaridade, permitindo analisar o espaço público em termos de permanências e descontinuidades.This research focuses on sociability in the public space of Avenida Getúlio Vargas in the municipality of Chapecó. It is a result of ethnographic research in the street that had the focus of analysis practices that define, fragment and create posts in the public space chapecoense. Are presented different social contexts, different scenarios that harbor from the tour - as a form of customary use of public space that appears in central part of the avenue - the ballads in the street - established practices of young people who gather in certain spaces, create scenarios and points of meeting. These places are made of specific dynamics of social, analyzed in this work, around the relations of the "see and be seen". The different urban sociability identified on Avenida Getúlio Vargas emphasized the importance of this as an icon of public space in the city of Chapecó and the surrounding physical and symbolic of different sociability. Of the scenarios examined, the prolonga appears as paradoxical space, surrounded both by connotations of transgressive and permissiveness. Ambiguous relationships that are organized in terms of coexistence, of simultaneity and complementarity, consider allowing the public space in terms of permanence and discontinuities
Entre arte(s) e poder(es): narrativas sobre a nação no contexto dos 500 anos
Get PDFDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas. Programa de Pós-Graduação em Antropologia Social.Esta dissertação investiga eventos ligados às comemorações dos 500 anos do Brasil, ocorridos no ano de 2.000: a festa de Porto Seguro, a Mostra do Redescobrimento e um dos módulos que a compôs, a exposição #Negro de Corpo e Alma#. A análise gira em torno da interface entre os campos da arte, da política e do poder, especialmente articulados no contexto comemorativo. Reflete-se, a partir da observação da (re) construção de narrativas, que contou com a inclusão ou exclusão de gêneros discursivos diversos (visuais, textuais e sonoros), sobre a atuação e a interação entre as diferentes instâncias presentes nos eventos: Estado, Imprensa, Intelectuais e Público.This dissertation investigates events related to Brazil#s 500 years celebrations, which happened in 2000: Porto Seguro party, Brazil Rediscovery Exhibition and one of its modules, #Black in body and Soul# Exposition. The analysis involves the interface among art, politics and power, specifically articulated in the celebration context. It is based on narrative re-construction, considering the inclusion and exclusion of several discursive genres (visual, textual and sonorous), about the acting and interaction between different instances in the events: State, Press, Intellectuals and Public
Where metaphors
No full textEste proyecto de grado, titulado "Metáforas del Dónde", fue realizado por María Camila Vejarano Fuentes para el programa de Artes Plásticas de la Universidad El Bosque en Bogotá, Colombia, en 2024. El trabajo explora el concepto de pertenencia y lugar a través de una práctica curatorial no convencional, cuestionando las nociones tradicionales de exposición artística. La autora colabora con cuatro artistas para investigar la pregunta "¿A dónde perteneces?", utilizando "no-diálogos" como método de reflexión. El proyecto se desarrolla en "Visaje Grafiti", una galería de grafiti en Bogotá, donde se invierte la presentación tradicional para dar más énfasis al proceso creativo que a las obras finales. Basándose en conceptos teóricos de Marc Augé y otros, el texto examina ideas de lugar, no lugar, identidad y alteridad, reflexionando sobre la condición de "foráneo" y la migración en el contexto colombiano. En última instancia, el proyecto propone una visión del arte como forma de exploración etnográfica y busca generar nuevas perspectivas sobre la pertenencia y la identidad en el ámbito artístico y social.Maestro en Artes PlásticasPregradoThis degree project, entitled "Metáforas del Dónde", was carried out by María Camila Vejarano Fuentes for the Fine Arts program at Universidad El Bosque in Bogotá, Colombia, in 2024. The work explores the concept of belonging and place through an unconventional curatorial practice, questioning traditional notions of artistic exhibition. The author collaborates with four artists to investigate the question "Where do you belong?", using "non-dialogues" as a method of reflection. The project takes place in "Visaje Grafiti", a graffiti gallery in Bogota, where the traditional presentation is inverted to give more emphasis to the creative process than to the final works. Drawing on theoretical concepts of Marc Augé and others, the text examines ideas of place, non-place, identity and otherness, reflecting on the condition of "outsider" and migration in the Colombian context. Ultimately, the project proposes a vision of art as a form of ethnographic exploration and seeks to generate new perspectives on belonging and identity in the artistic and social realm.
Translated with DeepL.com (free version
Prevalência de comprometimento cognitivo sem demência e demência em Tremembé, Brasil
No full textMade available in DSpace on 2019-09-12T16:53:51Z (GMT). No. of bitstreams: 0
Previous issue date: 2016Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Contexto: A prevalência de comprometimento cognitivo é insuficientemente determinada em países em desenvolvimento. O objetivo deste estudo foi verificar a prevalência de comprometimento cognitivo sem demência e demência em idosos residentes na comunidade no Brasil. Métodos: Trata-se de um inquérito transversal, monofásico, com idosos (60 anos ou mais) residentes no município de Tremembé, Brasil. Vinte por cento dos domicílios com idosos foram selecionados aleatoriamente em áreas urbanas e rurais, para obter uma representação homogênea de todos os níveis socioeconômicos e culturais. Resultados: Avaliamos 630 indivíduos [idade média, 71,3 anos (± 7,99); média de anos de educação, 4,9 (± 4,54)] e encontraram taxas de prevalência de 17,5% (intervalo de confiança de 95%, 14,6-20,6) para demência e 19,5% (intervalo de confiança de 95%, 16,6-22,8) para comprometimento cognitivo sem demência. Essas taxas de prevalência foram influenciadas pela idade (P <0,001) e pelo nível de escolaridade (P <0,001). Não houve diferença significativa entre os sexos entre os grupos de diagnóstico (P = 0,166). A prevalência de demência foi maior em indivíduos relativamente mais jovens (abaixo de 70 anos) quando comparada com outros estudos. Além disso, a demência foi associada a baixo nível socioeconômico, acidente vascular cerebral, transtorno psiquiátrico prévio, alcoolismo e epilepsia. Conclusões: A prevalência de demência neste estudo foi maior do que em outros estudos, principalmente em idosos mais jovens.Background:The prevalence of cognitive impairment is insufficiently determined in developing countries. The aim of this study was to ascertain the prevalence of cognitive impairment without dementia and dementia in community-dwelling elderly in Brazil.Methods:This was a single-phase cross-sectional survey of the elderly (aged 60 years and above) living in the municipality of Tremembe, Brazil. Twenty percent of the households with elderly persons were randomly selected from urban and rural areas, to obtain a homogenous representation of all socioeconomic and cultural levels.Results:We assessed 630 individuals [mean age, 71.3 y (7.99); mean years of education, 4.9 (+/- 4.54)] and found prevalence rates of 17.5% (95% confidence interval, 14.6-20.6) for dementia and 19.5% (95% confidence interval, 16.6-22.8) for cognitive impairment without dementia. These prevalence rates were influenced by age (P<0.001) and by educational level (P<0.001). There was no significant sex difference among diagnostic groups (P=0.166). The prevalence of dementia was higher in relatively younger individuals (below 70 y) when compared with other studies. Besides, dementia was associated with low socioeconomic status, stroke, previous psychiatric disorder, alcoholism, and epilepsy.Conclusions:The prevalence of dementia in this study was higher than in other studies, particularly among younger elderly.[Cesar, Karolina G.; Brucki, Sonia M. D.; Takada, Leonel T.; Oliveira, Maira O.; Porto, Fabio H. G.; Senaha, Mirna L. H.; Bahia, Valeria S.; Silva, Thais B. L.; Ianof, Jessica N.; Spindola, Livia; Schmidt, Magali T.; Jorge, Mario S.; Vale, Patricia H. F.; Cecchini, Mario A.; Cassimiro, Luciana; Soares, Roger T.; Goncalves, Marcia R.; Martins, Ana C. S.; Dare, Patricia; Smid, Jerusa; Porto, Claudia S.; Carthery-Goulart, Maria T.; Yassuda, Monica S.; Mansur, Leticia L.; Nitrini, Ricardo] Univ Sao Paulo, Dept Neurol, Cognit & Behav Neurol Unit, Sao Paulo, Brazil[Cesar, Karolina G.; Nascimento, Luiz F. C.; Gomes, Camila M. S.; Almeida, Milena C. S.] Universidade de Taubaté (Unitau
Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons.
Get PDF© The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Cutaneous secretions of amphibians have bioactive compounds, such as peptides, with potential for biotechnological applications. Therefore, this study aimed to determine the primary structure and investigate peptides obtained from the cutaneous secretions of the amphibian, Leptodactylus vastus, as a source of bioactive molecules. The peptides obtained possessed the amino acid sequences, GVVDILKGAAKDLAGH and GVVDILKGAAKDLAGHLASKV, with monoisotopic masses of [M + H]± = 1563.8 Da and [M + H]± = 2062.4 Da, respectively. The molecules were characterized as peptides of the class of ocellatins and were named as Ocellatin-K1(1-16) and Ocellatin-K1(1-21). Functional analysis revealed that Ocellatin-K1(1-16) and Ocellatin-K1(1-21) showed weak antibacterial activity. However, treatment of mice with these ocellatins reduced the nitrite and malondialdehyde content. Moreover, superoxide dismutase enzymatic activity and glutathione concentration were increased in the hippocampus of mice. In addition, Ocellatin-K1(1-16) and Ocellatin-K1(1-21) were effective in impairing lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) formation and NF-kB activation in living microglia. We incubated hippocampal neurons with microglial conditioned media treated with LPS and LPS in the presence of Ocellatin-K1(1-16) and Ocellatin-K1(1-21) and observed that both peptides reduced the oxidative stress in hippocampal neurons. Furthermore, these ocellatins demonstrated low cytotoxicity towards erythrocytes. These functional properties suggest possible to neuromodulatory therapeutic applications.Alexandra Plácido is a recipient of a post-doctoral grant from the project FCT (PTDC/BII-BIO/31158/2017). Renato Socodato and Camila Cabral Portugal hold postdoctoral fellowships from FCT (Refs: SFRH/BPD/91833/2012 and FRH/BPD/91962/2012, respectively). This work was funded through project UID/QUI/50006/2013-POCI/01/0145/FEDER/007265 (LAQV/REQUIMTE) with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT 2020info:eu-repo/semantics/publishedVersio
Recobrimento radicular: enxerto autógeno ou alógeno? Revisão de literatura e casos clínicos comparativos
Get PDFTCC (graduação) - Universidade Federal de Santa Catarina. Centro de Ciências da Saúde. Odontologia.Recessão gengival é uma característica comum tanto entre populações com higiene bucal precária, bem como nas de alto padrão de higiene bucal. A escovação dental traumática é um dos fatores etiológicos mais prevalentes. Tais alterações são tratadas, principalmente, por questão estética e de hipersensibilidade. Opções de recobrimento radicular como, por exemplo, as que utilizam ETCS (autógena) ou MDA (alógena – Alloderm®) são técnicas constantemente estudadas. Baseado nisso, o presente estudo teve o objetivo principal de discutir possíveis diferenças entre os resultados obtidos através das técnicas de enxerto (autógena x alógena) para recobrimento radicular, a partir de uma revisão de literatura e a apresentação de casos clínicos comparativos. Para iniciar a revisão foram definidos os descritores de Ciência da Saúde (DECS) para serem utilizados na estratégia de busca nos bancos de dados online (MedLine, PubMed). Alguns critérios foram definidos para refinar os resultados da busca, assim como a busca manual por meio de autores ou referências consideradas clássicas na literatura. Para cumprir o objetivo geral do presente trabalho, foram utilizados, para fim de comparação, casos clínicos da literatura e um caso clínico realizado na UFSC. Os resultados de recobrimento radicular alcançados neste caso foram: 86,4% de recobrimento nas áreas onde foram utilizados ETCS e de 49,8% com MDA, este último contrastando com os achados na literatura. Com isso, foi possível concluir que a técnica utilizando enxerto autógeno possui alta previsibilidade ao comparar-se com o alógeno, mas os resultados clínicos e estéticos são satisfatórios em ambas as técnicas, apresentando, além de recobrimento radicular, um aumento (em altura e espessura) de mucosa ceratinizada que fornece estabilidade à progressão das recessões e a possibilidade de uma segunda cirurgia plástica periodontal para atingir o recobrimento radicular.Gingival recession is a common feature both among populations with poor oral hygiene, as well as the high standard of oral hygiene. Traumatic tooth brushing is one of the most prevalent etiologic factors. Such changes are handled primarily for aesthetics and hypersensitivity. Options as root coverage, for example, using ETCS (autogenous) or MDA (allogeneic - Alloderm®) techniques are constantly studied. Based on this, the present study has the main objective to discuss possible differences between the results obtained through the techniques of graft (autogenous x allogeneic) for root coverage, from a literature review and presentation of comparative clinical cases. To begin to review the descriptors of Health Science (DECS) to be used in the search strategy in online databases (Medline, PubMed) were defined. Some criteria were defined to refine the search results, as well as a manual search by author or references considered classic literature. To meet the overall objective of this study were used for comparison purpose, clinical cases from the literature and a case study conducted at UFSC. The results of root coverage achieved in this case were: 86.4% of coverage in areas where ETCS were used and 49.8% with MDA, the latter contrasting with the findings in the literature. Thus, it was concluded that the technique using autogenous graft has high predictability when compared with the allograft, but the clinical and cosmetic results are satisfactory in both techniques, presenting, and root coverage, an increase (in height and thickness) of keratinized mucosa that provides stability to the progression of recessions and the possibility of a second plastic periodontal surgery to achieve root coverage
Between objects and art: the fears and processes of subjectivation - one reading of bojunguianas works
No full textThe present study, which focuses on the narratives A bolsa amarela, Corda bamba and
Seis vezes Lucas, by the author Lygia Bojunga, aims to analyze how objects and art are
means for overcoming fear and for the development of own subjectivity of the
children’s characters in these works. In this way, we will investigate the following
themes: fear and the unusual, art and memory, object/space and practices of
subjectivation. Therefore, the theoretical basis will be based on the theories of Michel
Foucault, Tzvetan Todorov, Remo Ceserani, Filipe Furtado, Sigmund Freud, Wolfgang
Iser, Gaston Bachelard, Marilena Chauí, Jean Delumeau, Jeanne Marie Gagnebin,
Marisa Martins Gama-Khalil, Maurice Halbwachs, Jacques Le Goff, J. R. R. Tolkien,
C. S. Lewis, Yi-Fu Tuan, among other theorists who work with such concepts.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorDissertação (Mestrado)No presente estudo, que tem como foco as narrativas A bolsa amarela, Corda bamba e
Seis vezes Lucas, da autora Lygia Bojunga, pretende-se analisar de que forma os objetos
e a arte são meios para a superação do medo e para o desenvolvimento da própria
subjetividade dos personagens infantis dessas obras. Desse modo, investigaremos os
seguintes temas: o medo e o insólito, a arte e a memória, o objeto/espaço e as práticas
de subjetivação. Para tanto, o embasamento teórico será realizado a partir das teorias de
Michel Foucault, Tzvetan Todorov, Remo Ceserani, Filipe Furtado, Sigmund Freud,
Wolfgang Iser, Gaston Bachelard, Marilena Chauí, Jean Delumeau, Jeanne Marie
Gagnebin, Marisa Martins Gama-Khalil, Maurice Halbwachs, Jacques Le Goff, J. R. R.
Tolkien, C. S. Lewis, Yi-Fu Tuan, entre outros teóricos que trabalham com tais
conceitos
Thiodina camilae Bustamante & Ruiz 2020, sp. nov.
No full textThiodina camilae sp. nov. Figs 9–10, 52–63 Etymology. The specific name honours Camila Cerón M.D., best female friend of the first author for more than 20 years, in recognition of her friendship, support, encouragement and patience. This also alludes to her short stature, like the representatives of this species. Diagnosis. Males and females of T. camilae sp. nov. resemble those of T. minuta (Galiano, 1977), T. perian Bustamante & Ruiz, 2017 and T. tyrioni sp. nov. by the general appearance (carapace yellow with black borders, a pair of brown stripes on the thoracic region, abdomen dorsally as in genus) and small body. Males of T. camilae sp. nov. differ from those of T. minuta and T. perian by the length of the embolus of the palp (360°), longer than the embolus of T. minuta (270°), but shorter than the embolus of T. perian (450°, 1T+90°) and T. tyrioni sp. nov. (480°, 1T+120°) [compare Figs 56 and 58 with Figs 68 and 70, Galiano (1977b: fig. 14) and Bustamante & Ruiz (2017: fig. 26A)]. The females resemble those of T. minuta, T. perian and T. tyrioni sp. nov. by the rounded shape of the spermathecae, but differ by the longer sclerotized portion of the copulatory ducts [compare Figs 62–63 with Figs 74–75, Galiano (1977b: fig. 17) and Bustamante & Ruiz (2017 fig. 26F)]. Description. Male (Holotype, UBC-SEM). Total length 1.84. Carapace length 0.88, width 0.59, height 0.36. AME diameter 0.19. Ocular quadrangle length 0.51. Anterior eye row width 0.56. Posterior eye row width 0.61. Abdomen length 0.96. Chelicera paturon: spine-like setae absent; promargin with one tooth, tricuspid, middle cusp larger than others, retromargin with two teeth, both curved. Excavation of chelicera absent. Leg I: femur 0.42x0.18, patella 0.24, tibia 0.29, metatarsus 0.23, tarsus 0.20; II: fe 0.34, pa 0.19, ti 0.21, mt 0.18, ta 0.17; III: fe 0.32, pa 0.17, ti 0.19, mt 0.19, ta 0.19; IV: fe 0.45, pa 0.20, ti 0.31, mt 0.26, ta 0.21. Leg formula 4123. Leg macrosetae: femur I–IV d1di, p0, r0; patella I–IV 0; tibia I p0, r0, v1r-0-2a-0; II p0, r0, v1r-0-0; III–IV 0; metatarsus I–II p0, r0, v2-2; III p2di, r2di (r0 right), v0; IV p2di, r2di, v0. Palp (Figs 56–59): tibia wider than long, RTA triangular, RvTA rounded (Figs 57, 59), PTA rounded (Fig. 58); embolus simple, fixed to tegulum, arising distally (12:00), with path of 360° before distal coil (Figs 56, 58). Color in alcohol: carapace yellow with black borders, two brown stripes on thoracic region, abdomen dorsum yellow, with three longitudinal dark stripes, sides with one longitudinal dark stripe (five stripes in total), venter yellow, spinnerets yellow. Femur I yellow, patella, tibia and metatarsus dark; other legs yellow (Figs 52–53). Female (Paratype, UBC-SEM). Total length 1.85. Carapace length 0.91, width 0.60, height 0.38. AME diameter 0.21. Ocular quadrangle length 0.53. Anterior eye row width 0.62. Posterior eye row width 0.65. Abdomen length 0.95. Chelicera paturon: promargin with two teeth, distal one bicuspid and curved, other unicuspid and curved, retromargin with one bicuspid and curved tooth. Leg I: femur 0.38x0.16, patella 0.24, tibia 0.26, metatarsus 0.19, tarsus 0.18; II: fe 0.34, pa 0.22, ti 0.20, mt 0.16, ta 0.16; III: fe 0.32, pa 0.18, ti 0.19, mt 0.17, ta 0.18; IV: fe 0.45, pa 0.20, ti 0.28, mt 0.23, ta 0.19. Leg formula 4123. Leg macrosetae: femur I–III d1di, p0, r0; IV d0-1-1, p0, r0; patella I–IV 0; tibia I p0, r0, v2-1r-0 (v2-2-0 right); II p0, r0, v1r-1r-0; III 0; IV p0, r0, v1pdi (v0 right); metatarsus I p0, r0, v2-2; II p0, r0, v1r-2; III p2di, r1di, v0; IV p1di, r2di (r1di right), v0. Epigyne (Figs 60–63): posterior border without lobes, copulatory openings U-shaped and posteriorly placed (Figs 60–61), copulatory ducts projected posteriorly to epigynal border (Figs 62–63). Color in alcohol: as in male, except for legs yellow and carapace lighter (Figs 54–55). Spine variation (one Ƌ, paratype). Femur I p1di; II d0-1-1, p1di; III: p1di; IV d1-1-1; Tibia I v1r-0-2a-1p; II v1r-1r-0; IV v2di; metatarsus II p1di. Type material. Holotype Ƌ: DOMINICAN REPUBLIC: Barahona: Parque Nacional Sierra Martín García, 18.424°N, 71.112°W, 170 m a.s.l., leg. W.P. Maddison, G.B. Edwards, J. Zhang & G. R. S. Ruiz, 21.VII.2009 (UBC-SEM). Paratypes: DOMINICAN REPUBLIC: Barahona: Parque Nacional Sierra Martín García, 18.424°N, 71.112°W, 170 m a.s.l., leg. W.P. Maddison, G.B. Edwards, J. Zhang & G. R. S. Ruiz, 21.VII.2009, 1♀ (UBC-SEM). Pedernales: Pedernales, 17.964°N, 71.652°W), 13 m a.s.l., leg. W. Maddison, G.B. Edwards, J. Zhang, G. R.S. Ruiz & N. Corona, 18.VII.2009, 3♀ (UBC-SEM); E of Pedernales, 17.965°N, 71.635°W, 30 m a.s.l., leg. W.P. Maddison, G.B. Edwards, J. Zhang, G. R. S. Ruiz & N. Corona, 1Ƌ 1♀ (UBC-SEM). Other material examined. DOMINICAN REPUBLIC: Pedernales: E of Pedernales, 17.965°N, 71.635°W, 30 m a.s.l., leg. W.P. Maddison, G.B. Edwards, J. Zhang, G. R. S. Ruiz & N. Corona, 17.VII.2009, 3 imm. (UBC-SEM); near Pedernales, 17.964°N, 71.652°W, 13 m a.s.l., leg. W.P. Maddison, G.B. Edwards, J. Zhang, G. R. S. Ruiz & N. Corona, 18.VII.2009, 3 imm. (UBC-SEM). Distribution. Known only from the type locality, Dominican Republic.Published as part of Bustamante, Abel A. & Ruiz, Gustavo R. S., 2020, New species and records of thiodinines from North and South America (Araneae Salticidae: Salticinae: Thiodinini), pp. 115-140 in Zootaxa 4899 (1) on pages 131-134, DOI: 10.11646/zootaxa.4899.1.6, http://zenodo.org/record/440075
Transcriptomic Analysis of Fludioxonil Resistance Mechanisms in Botrytis cinerea
No full textBotrytis cinerea is a destructive plant pathogen that infects a wide range of economically important crops. Limiting pathogen infection during production and after harvest is largely dependent on fungicide applications; fungicide-resistant isolates of B. cinerea have been recovered from various hosts. Resistance of B. cinerea to fludioxonil has been associated with overexpression of transporter genes (BcatrB and mfsM2) and mutations on histidine kinase proteins (Bos1, Bchhk2, Bchhk17). To identify possible mechanisms associated with fludioxonil resistance, the genomic expression of three sensitive and three low-resistant isolates was studied. Overexpression of BcatrB was observed when comparing low-resistant and sensitive isolates but was not specific to the fludioxonil treatment. Seven amino acid substitutions and one deletion were identified in the transcription factor Bcmrr1 in low-resistant isolates, associated with overexpression of BcatrB. The L497 deletion, previously associated with highly resistant isolates (MDR1h), was observed in two low-resistant isolates. Other differentially expressed genes associated with transmembrane transport, oxidoreductase activity, and lipid metabolic processes could be key in understanding the fungicidal mechanism(s) of fludioxonil. Expression profiles were isolate-specific. Following fludioxonil exposure, two sensitive isolates of B. cinerea sensu stricto showed a change in gene expression levels associated with cell membrane and peroxidase activity. In one low-resistant isolate of B. cinerea group S, fludioxonil exposure resulted in the overexpression of stress response genes and MFS transporter Bcstl1; one sensitive and two low-resistant isolates showed no significant changes in gene expression profiles. This work provides insight into the effect of fludioxonil on B. cinerea and potential fungicide resistance mechanisms. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license
Femoral Physeal-Sparing ACL Reconstruction With Iliotibial Band Autograft Over-the-Top With Associated Lateral Extra-Articular Tenodesis
No full textBackground: Injuries to the anterior cruciate ligament (ACL) in the pediatric population have been exponentially increasing over the years. However, surgical techniques typically employed for ACL reconstruction (ACL-R) in adults may injure the physes of skeletally immature patients, resulting in growth disturbances. Indications: Currently, ACL-R is recommended for most patients, aiming to return the patient to their previous activities as well as reduce the risk of further instability, meniscal and chondral injuries, and early osteoarthritis. Pediatric ACL-R techniques may vary widely. In this video, the technique chosen was over-the-top on the femur with a vertical tibial tunnel, in addition to a lateral extra-articular tenodesis, using the iliotibial (IT) band as the graft. Technique Description: The patient is positioned in standard supine arthroscopic position. An incision is made over the lateral epicondyle and the IT band is exposed. A 2-cm-wide graft is harvested. The proximal aspect of the graft is truncated as high as possible, and the distal aspect is left attached to Gerdy tubercle. Arthroscopic portals are established, and the remanent ACL is debrided, exposing the posterior aspect of the lateral femoral condyle. The capsule is penetrated using a Schnidt tonsil to establish the over-the-top position, which is subsequently exchanged for a cardiac clamp. The graft sutures are grasped using the clamp and pulled into the joint. The tibial tunnel is drilled at the anatomic footprint of the ACL. The graft is threaded over-the-top of the lateral femoral condyle and through the tibial tunnel. Finally, femoral graft fixation is performed on the lateral femoral condyle using interrupted sutures through the IT band and periosteum, and tibial graft fixation is performed with standard interference fixation. Results: Previous literature shows low re-rupture rates, excellent postoperative patient-reported outcomes, and high return to sport rates. Conclusion: In the pediatric population, there is still no ACL-R technique defined as the gold standard. The described technique is a valuable option for ACL-R in skeletally immature patients, with low revision rates and excellent postoperative outcomes. In addition, this technique minimizes the risk of growth disturbances and effectively stabilizes the knee, allowing patients to return to previous activities. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication
- …
