655 research outputs found
Interview with Ruba Salih, by Nadeem Karkabi and Sonia Boulos
No full textOn the occasion of publishing a special issue of Palestine/
Israel Review (PIR) on the decolonization of the city of Haifa, PIR interviewed Ruba Salih, a professor of anthropology at the Department of
Arts, University of Bologna. Salih’s research focuses on transnational
migration and diasporas across Europe, the Middle East, and North
Africa; Islam and gender; the Palestine question and refugees; and
trauma and conflict in the Middle East. She is the author of numerous academic works, including the book Gender in Transnationalism:
Home, Longing and Belonging among Moroccan (Routledge, 2003), and
the article “Bodies That Walk, Bodies That Talk, Bodies That Love: Palestinian Women Refugees, Affectivity, and the Politics of the Ordinary” (Antipode, 2017). Currently, she is working on a book on waiting and the politics of return among Palestinian refugees (Cambridge University Press). Ruba herself is the daughter of a Palestinian refugee from
Haifa, and her mother was born in Yafa/Jaffa. PIR held an interview
with her to talk about her work and her own experience of refuge as a
descendant of Palestinian refugees from Haifa. Nadeem Karkabi and
Sonia Boulos conducted the interview for PIR
Nerve sheath tumor with degenerative atypia and multivacuolated lipoblasts
No full textLipoblastic nerve sheath tumor is a recently described benign soft tissue tumor consisting of lipoblasts in a neural-schwannian background. The 6 reported cases have exclusively comprised signet ring lipoblasts and showed no cellular atypia. The authors describe the first lipoblastic nerve sheath tumor to harbor multivacuolated lipoblasts and degenerative atypia, underscoring its important differential diagnosis with well-differentiated liposarcoma. The purpose of this report is to expand the morphologic spectrum of this unusual neoplasm, and reemphasize the potential of benign nonadipocytic tumors to harbor multivacuolated lipoblasts and mimic liposarcoma. © 2012 The Author(s).Ayad T, 2007, EUR ARCH OTO-RHINO-L, V264, P93, DOI 10.1007-s00405-006-0119-6; Ceballos KM, 1999, ARCH PATHOL LAB MED, V123, P941; Chen E, 2010, AM J SURG PATHOL, V34, P707, DOI 10.1097-PAS.0b013e3181d74bdb; Coffin CM, 2009, AM J SURG PATHOL, V33, P1705, DOI 10.1097-PAS.0b013e3181b76462; Dei Tos Angelo P., 1994, American Journal of Surgical Pathology, V18, P913, DOI 10.1097-00000478-199409000-00006; Fukunaga M, 1998, PATHOL INT, V48, P562, DOI 10.1111-j.1440-1827.1998.tb03950.x; Hornick JL, 2004, AM J SURG PATHOL, V28, P1257, DOI 10.1097-01.pas.0000135524.73447.4a; Khalifa MA, 2000, AM J CLIN PATHOL, V114, P123; Lae ME, 2002, HISTOPATHOLOGY, V40, P505, DOI 10.1046-j.1365-2559.2002.01409.x; Mentzel T, 2010, MODERN PATHOL, V23, P729, DOI 10.1038-modpathol.2010.66; Plaza JA, 2006, AM J SURG PATHOL, V30, P337; Vecchio G M, 2010, Pathologica, V102, P108; Weiss SW, 2008, ENZINGER WEISSS SOFT; Weiss S W, 1996, Monogr Pathol, V38, P2070
Valutazione delle modificazioni biochimiche determinate dalla valinomicina nell’induzione sperimentale di edema corneale nel coniglio.
No full textÉtude des fonctions immunomodulatrices des lymphocytes T « Doubles-Négatifs »
No full textLa réaction du greffon contre l’hôte (GvH) est responsable d’un grand taux de morbidité et de mortalité chez les patients recevant des greffes de cellules souches (GCSH) allogéniques. Dans ce contexte, les cellules T régulatrices sont largement étudiées et semblent avoir un grand potentiel d’utilisation dans le domaine de la thérapie cellulaire de la GvH. Parmi les populations cellulaires T régulatrices, les lymphocytes T CD4-CD8- TCRαβ+ « Doubles-Négatifs » (DN), qui ne représentent que 1-3% des lymphocytes T, ont été décrits. Ces cellules ont des propriétés inhibitrices de la réponse immunitaire qui s’avèrent spécifiques aux antigènes auxquels elles ont préalablement été exposées. La répression de la réponse immunitaire par les cellules T DN régulatrices semble être un mécanisme important impliqué dans l’induction de la tolérance aux allo-antigènes. De plus, ces cellules confèrent une tolérance immunitaire dans des modèles de greffes allogéniques et xénogéniques. En effet, ces cellules ont la capacité d’inhiber la réaction contre un allo-antigène auquel elles ont été exposées, sans inhiber la réaction contre un allo-antigène inconnu. Les cellules T DN ont été isolées et caractérisées chez l’homme où elles ont la capacité d’interagir avec des cellules présentatrices d’antigènes (APCs) par un contact cellulaire, comme chez la souris. Cependant, leur capacité immunomodulatrice reste inconnue chez l’humain. Notre objectif consistait donc principalement à étudier le rôle et le mécanisme d’action des cellules T DN régulatrices humaines in vitro, en étudiant leur capacité à inhiber une réaction lymphocytaire mixte (MLR).
Nous avons montré que les cellules T DN stimulées par un allo-antigène donné inhibent des cellules syngéniques effectrices dirigées contre ce même alloantigène mais n’inhibent pas des cellules syngéniques effectrices dirigées contre un autre alloantigène, démontrant ainsi la spécificité aux antigènes de ces cellules. De plus, les T DN non stimulées par un allo-antigène n’ont pas de rôle inhibiteur. Cependant, durant cette inhibition, nous n’observons pas de modulation de l’expression des marqueurs d’activation et d’induction de l’apoptose. Afin d’étudier le mécanisme d’action des cellules T DN, nous avons mesuré l’expression intracellulaire de la granzyme B. Les résultats démontrent que les cellules T DN stimulées expriment un niveau significativement plus élevé de granzyme B que les cellules T DN non-stimulées par l’allo-antigène. Ceci suggère que l’immunosuppression induite par les cellules T DN stimulées pourrait passer par la voie granzyme B. Le mécanisme utilisé par ces cellules reste à être confirmé par nos futures expériences.Graft-versus-Host Disease (GvHD) is a major cause of morbidity and mortality in patients receiving an allogeneic Hematopoietic Stem Cell Transplantation (HSCT). Many regulatory T cell populations have been studied and shown to have immunosuppressive properties in GvHD. Among these populations, Double Negative CD4-CD8-TCRαβ+ regulatory T cells (DN T) have been described. These cells represent 1-3% of all T cell lymphocytes and are known to have antigen-specific inhibitory functions of the immune response. The suppression of an immune response by DN T cells seems to be an important mechanism involved in the induction of tolerance to allo-antigens. Moreover, these cells also confer immune tolerance in models of allogeneic and xenogenic grafts. DN T cells have the ability to suppress syngeneic T CD4+ and T CD8+ cells in an antigen-specific manner. Therefore, these DN T cells can inhibit the reaction caused by syngeneic effector cells against a specific alloantigen to which they have been previously exposed. However, they cannot inhibit a reaction directed against an unknown alloantigen. Human DN T cells have been isolated and characterized as cells that have the capacity to interact with APCs by cell-to-cell contact, just like in mice. However, their immunomodulatory properties are still unknown in humans. The goal of our project was to study the role and immunomodulatory functions of human DN T cells in Mixed Lymphocyte Reactions (MLR).
The MLRs have allowed us to demonstrate that DN T cells, after having been stimulated by an allo-antigen, have an antigen-specific inhibitory function towards the syngeneic effector cells reacting against the same alloantigen that they have been exposed to. Interestingly, they do not inhibit the reaction of these effector cells against an unknown alloantigen. However, stimulated DN T cells did not modulate the expression of the activation markers expressed by the effector cells and did not give a death signal to these cells either. Moreover, we also wanted to study how DN T cells have an immunosuppressive activity. Therefore, we compared the expression of Granzyme B in stimulated versus non-stimulated cells. Our results suggest that DN T cells may use the Granzyme B pathway to immunosuppress the effector cells. In conclusion, our results demonstrate that DN T cells have an antigen-specific inhibitory function. The mechanism used by these DN T cells remains to be confirmed with our future experiments
Cynaropicrin disrupts tubulin and c-Myc-related signaling and induces parthanatos-type cell death in multiple myeloma
No full textThe majority of blood malignancies is incurable and has unforeseeable remitting-relapsing paths in response to different treatments. Cynaropicrin, a natural sesquiterpene lactone from the edible parts of the artichoke plant, has gained increased attention as a chemotherapeutic agent. In this study, we investigated the effects of cynaropicrin against multiple myeloma (MM) cells in vitro and assessed its in vivo effectiveness in a xenograft tumor zebrafish model. We showed that cynaropicrin exerted potent cytotoxicity against a panel of nine MM cell lines and two leukemia cell lines with AMO1 being the most sensitive cell line (IC50= 1.8 ± 0.3 μM). Cynaropicrin (0.8, 1.9, 3.6 μM) dose-dependently reduced c-Myc expression and transcriptional activity in AMO1 cells that was associated with significant downregulation of STAT3, AKT, and ERK1/2. Cell cycle analysis showed that cynaropicrin treatment arrested AMO1 cells in the G2M phase along with an increase in the sub-G0G1 phase after 24 h. With prolonged treatment times, cells accumulated more in the sub-G0G1 phase, implying cell death. Using confocal microscopy, we revealed that cynaropicrin disrupted the microtubule network in U2OS cells stably expressing α-tubulin-GFP. Furthermore, we revealed that cynaropicrin promoted DNA damage in AMO1 cells leading to PAR polymer production by PARP1 hyperactivation, resulting in AIF translocation from the mitochondria to the nucleus and subsequently to a novel form of cell death, parthanatos. Finally, we demonstrated that cynaropicrin (5, 10 μM) significantly reduced tumor growth in a T-cell acute lymphoblastic leukemia (T-ALL) xenograft zebrafish model. Taken together, these results demonstrate that cynaropicrin causes potent inhibition of hematopoietic tumor cells in vitro and in vivo
Étude des fonctions immunomodulatrices des lymphocytes T « Doubles-Négatifs »
No full textLa réaction du greffon contre l’hôte (GvH) est responsable d’un grand taux de morbidité et de mortalité chez les patients recevant des greffes de cellules souches (GCSH) allogéniques. Dans ce contexte, les cellules T régulatrices sont largement étudiées et semblent avoir un grand potentiel d’utilisation dans le domaine de la thérapie cellulaire de la GvH. Parmi les populations cellulaires T régulatrices, les lymphocytes T CD4-CD8- TCRαβ+ « Doubles-Négatifs » (DN), qui ne représentent que 1-3% des lymphocytes T, ont été décrits. Ces cellules ont des propriétés inhibitrices de la réponse immunitaire qui s’avèrent spécifiques aux antigènes auxquels elles ont préalablement été exposées. La répression de la réponse immunitaire par les cellules T DN régulatrices semble être un mécanisme important impliqué dans l’induction de la tolérance aux allo-antigènes. De plus, ces cellules confèrent une tolérance immunitaire dans des modèles de greffes allogéniques et xénogéniques. En effet, ces cellules ont la capacité d’inhiber la réaction contre un allo-antigène auquel elles ont été exposées, sans inhiber la réaction contre un allo-antigène inconnu. Les cellules T DN ont été isolées et caractérisées chez l’homme où elles ont la capacité d’interagir avec des cellules présentatrices d’antigènes (APCs) par un contact cellulaire, comme chez la souris. Cependant, leur capacité immunomodulatrice reste inconnue chez l’humain. Notre objectif consistait donc principalement à étudier le rôle et le mécanisme d’action des cellules T DN régulatrices humaines in vitro, en étudiant leur capacité à inhiber une réaction lymphocytaire mixte (MLR).
Nous avons montré que les cellules T DN stimulées par un allo-antigène donné inhibent des cellules syngéniques effectrices dirigées contre ce même alloantigène mais n’inhibent pas des cellules syngéniques effectrices dirigées contre un autre alloantigène, démontrant ainsi la spécificité aux antigènes de ces cellules. De plus, les T DN non stimulées par un allo-antigène n’ont pas de rôle inhibiteur. Cependant, durant cette inhibition, nous n’observons pas de modulation de l’expression des marqueurs d’activation et d’induction de l’apoptose. Afin d’étudier le mécanisme d’action des cellules T DN, nous avons mesuré l’expression intracellulaire de la granzyme B. Les résultats démontrent que les cellules T DN stimulées expriment un niveau significativement plus élevé de granzyme B que les cellules T DN non-stimulées par l’allo-antigène. Ceci suggère que l’immunosuppression induite par les cellules T DN stimulées pourrait passer par la voie granzyme B. Le mécanisme utilisé par ces cellules reste à être confirmé par nos futures expériences.Graft-versus-Host Disease (GvHD) is a major cause of morbidity and mortality in patients receiving an allogeneic Hematopoietic Stem Cell Transplantation (HSCT). Many regulatory T cell populations have been studied and shown to have immunosuppressive properties in GvHD. Among these populations, Double Negative CD4-CD8-TCRαβ+ regulatory T cells (DN T) have been described. These cells represent 1-3% of all T cell lymphocytes and are known to have antigen-specific inhibitory functions of the immune response. The suppression of an immune response by DN T cells seems to be an important mechanism involved in the induction of tolerance to allo-antigens. Moreover, these cells also confer immune tolerance in models of allogeneic and xenogenic grafts. DN T cells have the ability to suppress syngeneic T CD4+ and T CD8+ cells in an antigen-specific manner. Therefore, these DN T cells can inhibit the reaction caused by syngeneic effector cells against a specific alloantigen to which they have been previously exposed. However, they cannot inhibit a reaction directed against an unknown alloantigen. Human DN T cells have been isolated and characterized as cells that have the capacity to interact with APCs by cell-to-cell contact, just like in mice. However, their immunomodulatory properties are still unknown in humans. The goal of our project was to study the role and immunomodulatory functions of human DN T cells in Mixed Lymphocyte Reactions (MLR).
The MLRs have allowed us to demonstrate that DN T cells, after having been stimulated by an allo-antigen, have an antigen-specific inhibitory function towards the syngeneic effector cells reacting against the same alloantigen that they have been exposed to. Interestingly, they do not inhibit the reaction of these effector cells against an unknown alloantigen. However, stimulated DN T cells did not modulate the expression of the activation markers expressed by the effector cells and did not give a death signal to these cells either. Moreover, we also wanted to study how DN T cells have an immunosuppressive activity. Therefore, we compared the expression of Granzyme B in stimulated versus non-stimulated cells. Our results suggest that DN T cells may use the Granzyme B pathway to immunosuppress the effector cells. In conclusion, our results demonstrate that DN T cells have an antigen-specific inhibitory function. The mechanism used by these DN T cells remains to be confirmed with our future experiments
CaCu3Ti4O12 ceramics from co-precipitation method: Dielectric properties of pellets and thick films
No full textDielectric properties of CaCu3Ti4O12 (CCTO)-based ceramics and thick films (e ∼50m) prepared from powders synthesized by a soft chemistry method (co-precipitation) are presented and discussed. The characteristics of pellets and thick films are compared. The pellets exhibit high values of the dielectric permittivity (εr ∼1.4×105) and relatively small dielectric losses (tan δ ∼0.16) at 1 kHz and room temperature. These properties are independent of the nature of the metallization of the electrodes. In addition, the dielectric permittivity decreases when the diameter of the electrodes of the pellets increases, while the losses remain constant. This result, which is strongly related to the nature of the dielectric material in between the electrodes, constitutes a strong indication that the high dielectric permittivity values observed in this material are not related to an interfacial (electrode material) related mechanism but is an internal barrier layer capacitor (IBLC) type. Very high values of the dielectric permittivity of CCTO thick films are measured (εr ∼5×104). The differences in dielectric permittivity between thick films and dense pellets may be attributed to the difference in grain size due to different CuO contents, and to the different reactivity of the materials
A representação das mulheres na primeira república (1889-1930) no livro didático: história, sociedade e cidadania de Alfredo Boulos
No full textThis work presents an analysis of the representations of women in historical narratives
about the First Brazilian Republic published in the textbook History, Society and
Citizenship (9th year) authored by Alfredo Boulos (2018), approved for the PNLD 2020.
It is intended to this analysis, discuss and problematize the meanings, values,
interests, functions and conditions of insertion of these representations in didactic
narratives and in history teaching. The book analyzed presents innovations in bringing
women as protagonists of History, however it still maintains certain silences and
problems in the way it inserts women in History. This research will address elements
about women's history and how it is represented in the aforementioned teaching
manual, through content and illustrations. Thus, the aim is to highlight the importance
of expressing this theme in textbooks to promote the formation of identities in history
teaching, seeking to break with violence and gender prejudice in all social spheres, so
that there is an education without inequalities and equal to all social groups.Este trabalho apresenta uma análise das representações das mulheres nas narrativas
históricas sobre a Primeira República brasileira veiculadas no livro didático História,
Sociedade e Cidadania (9° ano) de autoria de Alfredo Boulos (2018), aprovado para
o PNLD 2020. Pretende-se com essa análise, discutir as formas de representação das
mulheres na Primeira República (1889-1930), no livro didático: História, sociedade e
cidadania, do autor Alfredo Boulos (2018), nos aspectos civis, sociais e político. O livro
analisado apresenta inovações ao trazer mulheres como protagonistas da História,
porém ainda mantém certos silenciamentos e problemáticas no modo como insere as
mulheres na História. Essa pesquisa abordará elementos acerca da história das
mulheres e em como ela estão sendo representadas no manual didático já
mencionado, através dos conteúdos e ilustrações. Assim, pretende-se nesse trabalho
evidenciar a importância da manifestação dessa temática nos livros didáticos para
promover ao ensino de história a formação das identidades, buscando romper com as
violências e preconceitos de gênero em todas as esferas sociais, para que haja uma
educação sem desigualdades e igualitária a todos os grupos sociais
Reproducibility of wax interocclusal records on different articulators
No full textContext: The construction of a dental prosthesis needs the duplication of intermaxillary relationships of the patient on the articulator. This procedure is normally fulfilled using interocclusal records to program the articulator according to the patient's condylar inclinations. Wax is a popular recording material used for this purpose but has proven to suffer from inaccuracies due to its dimensional variation over time. Aims: This study intends to test the reproducibility of eccentric interocclusal wax records over time. Setting and Design: Thirteen dental students aged between 18 and 30 years participated in this study. Maxillary and mandibular casts of the participants were mounted on two types of articulators; wax lateral and protrusive interocclusal records were used to program these articulators. Subjects and Methods: Horizontal and lateral condylar inclinations were obtained for each participant. The interocclusal records were stored for 10 days and the casts of each participant were remounted on the articulators. The interocclusal records were reused to obtain new condylar inclinations of each participant. The initial and the delayed condylar inclinations were compared to evaluate the impact of storage time on wax records. Statistical Analysis Used: Paired Student's t-test was used for this comparison. Results: No significant difference (0.38 0.600). Conclusions: The use of wax interocclusal records for articulator programing was shown to be acceptable even with delayed intervals, without concerns of possible variations of condylar settings due to storage time
Clinical and biological significance of E-cadherin protein expression in invasive lobular carcinoma of the breast
No full text[No abstract available]Esposito NN, 2007, MODERN PATHOL, V20, P130, DOI 10.1038-modpathol.3800721; Qureshi HS, 2006, AM J CLIN PATHOL, V125, P377, DOI 10.1309-WMX7DRWTFVQP2LOT; Rakha EA, 2005, HISTOPATHOLOGY, V46, P685, DOI 10.1111-j.1365-2559.2005.02156.x; Rakha EA, 2010, AM J SURG PATHOL, V34, P1472, DOI 10.1097-PAS.0b013e3181f01916; Tavassoli FA, 2003, WHO CLASS TUM PATH G, P23; Wheeler DT, 2004, AM J SURG PATHOL, V28, P1587, DOI 10.1097-00000478-200412000-0000611
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