1,458 research outputs found
Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba
No full textArchaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly to dsDNA (double-stranded DNA) than to either ssDNA (single-stranded DNA) or RNA. The Alba protein is dimeric in solution, and forms distinct ordered complexes with DNA that have been visualized by electron microscopy studies; these studies suggest that, on binding dsDNA, the protein forms extended helical protein fibres. An end-to-end association of consecutive Alba dimers is suggested by the presence of a dimer-dimer interface in crystal structures of Alba from several species, and by the strong conservation of the interface residues, centred on Are and Phe(60). In the present study we map perturbation of the polypeptide backbone of Alba upon binding to DNA and RNA by NMR, and demonstrate the central role of Phe(60) in forming the dimer dimer interface. Site-directed spin labelling and pulsed ESR are used to confirm that an end-to-end, dimer dimer interaction forms in the presence of dsDNA.Peer reviewe
First person – Alba Delrio-Lorenzo
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Alba Delrio-Lorenzo is first author on ‘Sarcoplasmic reticulum Ca2+ decreases with age and correlates with the decline in muscle function in Drosophila’, published in JCS. Alba is a PhD student in the lab of Javier García-Sancho and María Teresa Alonso at the Instituto de Biología y Genética Molecular (IBGM), University of Valladolid, Spain, investigating the molecular mechanisms implicated in aging, particularly muscle aging.Peer reviewe
The Author/Translator Interactional Process. A Case Study
No full textSee Naples and Kill (1988) is a lively and colourful novel by the con-temporary English writer, Gregory Dowling, translated into Italian in 2015.
Following the tradition of translation studies (Venuti 2000, Bass-nett 2002, Cronin 2006), this paper analyses the rewriting process of literary translation, considering in particular the fruitful but sometimes tense and even conflictual relationship between writer and translator.
The translation of the novel See Naples and Kill was an ongoing rewriting process entailing a constant dialogue between the writer and the translator. Therefore, the study aims at answering two main ques-tions: what happens if the rewriting process of translation is constant-ly questioned by the author? What happens if the author has a good mastery of the target language and s/he is her/himself a translator?
By exploring the relationship between translation and re-creation, the research focuses on the differences and similarities between the primary creation (source text) and the secondary creation (target text), and aims to verify in which way the dialogic encounter of two different personalities and cultures does not make them merge but, by retaining their own uniqueness, leads eventually to their mutually en-riching each other. A comparative analysis of the source text and the different drafts of the translated version accompanied by the author’s comments will shed light on the tense author-translator relationship in the specific case under investigation and how both actors handle this tension in order to create a new work resulting from the (dis)agreement of the two parties
A ALBA e a luta pela hegemonia no continente americano: uma topologia do discurso bolivariano
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas. Programa de Pós-graduação em Sociologia PolíticaO objetivo da dissertação é analisar o movimento bolivariano, liderado pela Venezuela, enquanto projeto de construção de uma nova hegemonia na América Latina e o Caribe. Tal projeto se constrói discursivamente em oposição à histórica hegemonia pan-americana liderada pelos Estados Unidos (EUA). Para isso é estudada a ALBA (Aliança Bolivariana para os Povos de nossa América) como organização internacional que facilita a alineação de países e sociedades latino-americanos em relação ao movimento bolivariano, gerando novas modalidades de construção de sentido, assim como novas vias e regras de relacionamento internacional. A pesquisa analisa os discursos de mandatários nas reuniões de Cúpula da ALBA, e, apelando à teoria do discurso de Ernesto Laclau, identifica nesse conjunto os três passos previstos pelo autor na configuração de um desafio hegemônico: (1) o estabelecimento de um antagonismo face ao imperialismo dos EUA que é identificado como o poder hegemônico institucionalizado, e a dicotomização do espaço social americano através da construção de uma fronteira entre esse poder e o resto da comunidade (América Latina e o Caribe); (2) a articulação contingente entre diversas demandas sociais, características da América Latina e do Caribe, cuja insatisfação é atribuída ao imperialismo dos EUA; e (3) a formação de um campo popular, através da expressão positiva dessas demandas numa nova identidade, no caso a identidade bolivariana. A pesquisa conclui que o movimento bolivariano consegue gerar elementos para permitir uma nova lógica de construção de sentido, configurando assim estruturalmente um campo de luta pela hegemonia. Porém, também são destacados vários problemas nessa tarefa, especialmente ligados à identificação de diversas unidades com o bolivarianismo.The dissertation research the Bolivarian Movement, headed by Venezuela, as a new hegemonic project, still under construction, at Latin America and the Caribbean. This hegemonic project is discursively constructed in opposition to the historic Pan American hegemony, led by the U.S. In this way, the Bolivarian Alliance for the Peoples of Our America (ALBA) is approached as an international organization that seeks to align the Latin American countries and societies behind the Bolivarian Movment. The ALBA generates new ways of sense-making, as well as new norms of international relations. The research analyzes the speeches at the ALBA's presidential summits, using the Ernesto Laclau's Discourse Theory, and identifying the three steps proposed by this author to the hegemonic construction: Firstly, the establishment of an antagonism against U.S. imperialism and the dicotomization of the hemispheric social space, through the definition of a boundary between the powerful country and the rest of continental community (Latina America and the Caribe). Secondly, the contingent articulation of a variety of social demands, which dissatisfaction are ascribed to the U.S. imperialism. Thirdly, the formation of a new popular field, through the positive expression on a new identity of this unified set of demands, in this case the Bolivarian identity. The dissertation concludes that the Bolivarian movement manages a new logic of sense-making, and therefore places the hegemonic struggle in a new structural field. However, it also identified some problems on this task, particularly in the difficulty to construct a Bolivarian identity shared by various social unities (countries, societies and social sectors)
Venezuela tra Alba e Mercosur. Un ruolo ambivalente
No full textGiacomo Pellini, "Venezuela tra Alba e Mercosur", in: Visioni LatinoAmericane, 11 (2014), pp.48-62L’autore esamina l’attivismo di Hugo Châvez nei processi di integrazione latinoamericana soffermandosi in particolare sull’Alba e il Mercosur. Mette a confronto, da una parte, la leadership esercitata dal Venezuela all’interno dell’Alba e dall’altra il ruolo di secondo piano del Venezuela all’interno del Mercosur. Evidenzia come per il Paese sia più vantaggiosa la permanenza, seppur con un ruolo meno incisivo, nel Mercosur che non la leadership nell’Alba. El autor examina el activismo de Hugo Châvez en los procesos de integración en América Latina, con especial énfasis en el Alba y en el Mercosur. Compara, por un lado, el liderazgo ejercido por Venezuela en el Alba y, por el otro, su rol secundario en el Mercosur. Muestra cómo para el Pais sea mâs ventajosa la retención, aunque con un rol de menor importancia, en el Mercosur, respecto al liderazgo en el Alba. The author examines the activism of Hugo Chàvez in the main integration processes in Latin America, Alba and Mercosur. He examines, on one hand, the leadership that Venezuela practices within the Alba and, on the other hand, the secondary role of Venezuela within Mercosur. He explains that for the country is more advantageous to remain inside the Mercosur, despite the secondary role, than to exercise the leadership within the Alba
Policy analysis and public policy in the private sector
No full textThis chapter provides a description of policy analysis in the private sector. The author introduces the topic with an informative description of the organizations that have been historically created to represent businessmen and businesswomen. Thereafter, Alba presents the main research centres that have been established by private actors with the aim of getting accurate information about political and economic trends. Alba then shows how business has developed some capacity to do policy analysis in the areas of its interest and explains the various mechanisms that businesses employ in their attempts to negotiate with state actors, and thus to influence policymaking processes.</p
Parfums de Philippe Claudel, un abécédaire des odeurs.
No full textAfter having investigated in many of his novels the human soul and his weaknesses, Philippe Claudel gives readers, in his latest book entitled Parfums, the possibility to follow him in a journey backwards, which recalls his olfactory memories. We will try to show how, starting with those reminiscences, the author brings us to discover his world. Philippe Claudel materialize those scents, whether pleasant or unpleasant into images, turning them into snapshots. It will be impossible in this paper to detail all the scents that inhabit the sensory world of Claudel, however, we will attempt to trace in this kaleidoscope of perfumes the aspects and issues with which the man and the writer must face
Femme du ciel et des tempêtes de Wilfries N'Sondé: récit initiatique et préhistorique pour dire l'urgence écologique
No full textThe interest of contemporary French literature in ecology and environment is now well established. It is undeniable that we are witnessing a generation of writers increasingly attuned to ecological and environmental issues. Consequently, literary criticism aims to read these works through the lens of ecocriticism, and more often, ecopoetics, which differs from the former by displaying a greater sensitivity toward the form and aesthetics of the text. Criticism focusing on environmental issues has often overlooked much of Francophone literature, and only in recent years has attention shifted toward this body of work. The study presented here examines the novel Femme du ciel et des tempêtes (2021) by Franco-Congolese writer Wilfried N’Sondé. It will highlight not only the major challenges posed by environmental writing but also the stylistic choices adopted by the author to craft a narrative structure that effectively conveys ecological concerns
Regionalism and South American orientation: UNASUR and ALBA
No full textThis study examines whether or not integration in South America is shaping a new type of regionalism. The author takes the cases of UNASUR and ALBA and shows – on the basis of the characteristics of institutionality, leaderships and identities – what the incentives for cooperation are. One of the essential elements for all integration is the defence agreements that are articulated in different ways in each of the organisations. The article attempts to show that both UNASUR and ALBA were structured using a new logic, by prioritising political agreements over commercial ones. The author describes a new South American notion that is still under construction, but which is more narrative- and discourse-oriented than involving formalised actions. As a result, we cannot conclude that this represents a new type of regionalism
Antagonistic effects of frugivores on seeds of Cryptocarya alba (Mol.) Looser (Lauraceae): consequences on seedling recruitment
No full textFrugivores affect Cryptocarya alba seeds both positively and negatively by the simple act of removing the pericarp of the fruits. Germination is both increased and advanced but simultaneously seed mortality by rodent predation is increased as well. The net effect of frugivores on germination is zero while in terms of seedling recruitment, it is positive. The ecological conditions for a mutualistic interaction between frugivores and C. alba are fulfilled. -Author
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