323,671 research outputs found
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
No full textReal-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
Citation:
Pulikkot, S., Zhao, M., Fan, Z. Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils. J. Vis. Exp. (196), e64971, doi:10.3791/64971 (2023)
Intraoral Non-hodgkin′s Lymphoma In a HIV Patient
No full textNon-Hodgkin′s lymphoma (NHL) is one of the most common malignancies associated with HIV infection. AIDS related NHL are primarily of the B cell phenotype with histological evidence of intermediate or high grade malignancy. Treatment of AIDS related NHL remains more complicated than lymphoma in non-HIV infected population. A rare case of intra oral non-hodgkin′s lymphoma with its successful resolution is discussed in this article
A Study of Thermally Radiant Williamson Nanofluid Over an Exponentially Elongating Sheet with Chemical Reaction Via Homotopy Analysis Method
No full textDiffusive author(s), cohesive author: Analysis of S/N (1994)
No full textThis study indicates the ways in which various aspects of the author(s) are brought forth in Dumb type’s performance art, the S/N production. Previous research has suggested a non-hierarchical organization of Dumb type and the absence of a “privileged author” in Dumb type’s collaborative work, S/N. However, the results that I have investigated from member’s interviews on the creative process of S/N along with my analysis of the recorded images of S/N, indicate a different aspect of the author(s). First, S/N was created through, so to speak, the collective ideas of the members of Dumb type. Further, S/N has at least nine quotations from previous performances, installations, and printed writings, besides the work-in-progress technique. Explicating one of the “author functions” as given by Michel Foucault, each text has plural subjects of the author. However, it has been revealed from members’ interviews that Teiji Furuhashi had a decision-making role in selecting the members’ ideas within the performance. Since then, S/N has had plural subjects of creation; however, Furuhashi is one of the subjects of creation along with the “privileged author.” S/N has plural authors (diffusive authors) yet at the same time, it has a “privileged author,” Teiji Furuhashi (cohesive author)
Antioxidative properties of fermented poultry green bones
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Receptor localization and dynamics of murine natural killer cells at single cell level : using advanced fluorescence microscopy
No full textNatural Killer (NK) cells are immune cells and important for the defense against virally infected and malignant cells. NK cells are regulated by germline encoded activating and inhibitory receptors. Activating receptors specifically recognize ligands which are either encoded by infectious agents, or induced upon infection or cellular stress. Inhibitory receptors interact with self-ligands expressed on healthy cells, among them MHC class I. NK cells inspect the host cells by screening for alterations in activating and inhibitory ligand expression. The balance between input from activating and inhibitory receptors determines the NK cell response. NK cells undergo a process of functional maturation and acquisition of self-tolerance via sensing of the steady-state input through their receptors. This process is known as education. The cytotoxic activity of NK cells can be further increased by cytokines produced by other immune cells.The aim of this thesis was to characterize the differences in receptor dynamics and localization between NK cells based on either cytokine activation or educational status. Fluorescence based advanced microscopy techniques were used to quantitate receptor dynamics and spatial organization.In paper I, we investigated the influence of cytokine stimulation on the lateral diffusion of the inhibitory receptor Ly49A and its ligand MHC class I on NK cells within the cell membrane. The response to cytokine stimulation was heterogeneous among the NK cells. We characterized a subpopulation of NK cells with faster diffusion of both MHC class I and Ly49A. The receptor diffusion was established on primary NK cells using Fluorescence Correlation Spectroscopy. In paper II, a practical protocol for utilizing FCS on primary lymphocytes was presented. In Paper III, we showed that NKp46 and Ly49A were confined within microdomains on NK cells. The actin cytoskeleton and cholesterol composition of NK cells played important roles in initiating activating cell signaling. In Paper IV, we investigated the organization and clustering of activating and inhibitory receptors on educated and uneducated NK cells. We found that clusters of NKp46 and Ly49A were larger on uneducated NK cells. The nearest neighbour distances from activating to inhibitory receptors were not significantly different between educated and uneducated NK cells, thus the organization of inhibitory receptors in relation to the activating receptors do not seem to be of importance for the educational process.In summary, the findings in this thesis enlightens the importance of altered receptor dynamics and organization on NK cells depending on the state of activation and education. Furthermore, receptor dynamics could be an important aspect for understanding NK cell function.List of scientific papersI. Cytokines Induce Faster Membrane Diffusion of MHC Class I and the Ly49A Receptor in a Subpopulation of Natural Killer Cells. Sunitha Bagawath-Singh, Elina Staaf, Arie Jan Stoppelenburg, Thiemo Spielmann, Taku Kambayashi, Jerker Widengren and Sofia Johansson. Front Immunol. Feb 4;7:16. 2016. https://doi.org/10.3389/fimmu.2016.00016 II. Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy. Elina Staaf, Sunitha Bagawath-Singh, Sofia Johansson. J Vis Exp. 1;(120) 2017. https://doi.org/10.3791/54756 III. Educated Natural Killer Cells Exhibit a More Dynamic Movement of NKp46 and a Higher Confinement of the Ly49A Receptor. E. Staaf, P. N. Hedde, Sunitha-Bagawath Singh, J. Piguet, E. Gratton, S. Johansson. [Submitted]IV. Nano-structural Organization of Activating and Inhibitory Receptors in Murine Natural Killer cell Education. Sunitha Bagawath Singh, Jan Bergstrand, Daniel Rönnlund, Hans Blom, Jerker Widengren and Sofia Johansson. [Manuscript]</p
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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