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SUPERANDO O MUNDO: ENTREVISTA COM A DEFENSORA DE DIREITOS HUMANOS SUNITHA KRISHNAN
Get PDFSUPERANDO O MUNDO: ENTREVISTA COM A DEFENSORA DE DIREITOS HUMANOS SUNITHA KRISHNA
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
No full textReal-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
Citation:
Pulikkot, S., Zhao, M., Fan, Z. Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils. J. Vis. Exp. (196), e64971, doi:10.3791/64971 (2023)
Impact of type of social media and type of review on consumer perception of online reviews
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Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
The influence of international clinical placement experiences on careers of healthcare students: graduate and employer perspectives
Get PDFThe internationalisation agenda continues to be one of the key influences on the
patterns of work of UK higher education institutions. In order to enhance cultural
competencies for healthcare students, provisions are made by several UK
universities for students to complete a clinical placement abroad, referred to as
‘international clinical placement’ (ICP) in this thesis. Previous studies on ICP have
examined the experiences of students but research on the long-term influence of
these experiences on students’ careers is still scant. The research reported here
represents an initial attempt to capture accounts of these influences.
This study was designed to gain a holistic understanding of the long-term influences
of ICP on students’ careers drawing on the multiple perspectives of healthcare
graduates and employers. To the best of my knowledge, this is the first study that has
gained employers’ views as well as the views of graduates, when exploring the impact
of ICP on employability.
Qualitative in-depth semi-structured interviews were conducted with both graduates
and healthcare employers. A thematic analysis of the data identified four key themes:
practice culture, communication in clinical practice, approaches to employability and
career management. The attitude of participants towards the value of ICP for
developing cultural competencies was largely positive but it was difficult to determine
the extent to which an isolated experience such as ICP influenced their employability. The findings from this study did not suggest that ICP experiences had any greater
influence than UK placements in gaining employment.
The discourse around standards and scope of clinical practices abroad was found to
be the dominant feature in shaping students’ careers. Whilst appreciating the
differences between the clinical practice in the UK and other countries in Europe, both
the employers and graduates in this study held strongly to the ethnocentric views that
the standard and scope of professional practice is limited in most European countries
when compared to the UK. The outcomes of this research benefit multiple stake
holders including students, academics, clinical educators, professional bodies,
employers, and researchers
Receptor localization and dynamics of murine natural killer cells at single cell level : using advanced fluorescence microscopy
No full textNatural Killer (NK) cells are immune cells and important for the defense against virally infected and malignant cells. NK cells are regulated by germline encoded activating and inhibitory receptors. Activating receptors specifically recognize ligands which are either encoded by infectious agents, or induced upon infection or cellular stress. Inhibitory receptors interact with self-ligands expressed on healthy cells, among them MHC class I. NK cells inspect the host cells by screening for alterations in activating and inhibitory ligand expression. The balance between input from activating and inhibitory receptors determines the NK cell response. NK cells undergo a process of functional maturation and acquisition of self-tolerance via sensing of the steady-state input through their receptors. This process is known as education. The cytotoxic activity of NK cells can be further increased by cytokines produced by other immune cells.The aim of this thesis was to characterize the differences in receptor dynamics and localization between NK cells based on either cytokine activation or educational status. Fluorescence based advanced microscopy techniques were used to quantitate receptor dynamics and spatial organization.In paper I, we investigated the influence of cytokine stimulation on the lateral diffusion of the inhibitory receptor Ly49A and its ligand MHC class I on NK cells within the cell membrane. The response to cytokine stimulation was heterogeneous among the NK cells. We characterized a subpopulation of NK cells with faster diffusion of both MHC class I and Ly49A. The receptor diffusion was established on primary NK cells using Fluorescence Correlation Spectroscopy. In paper II, a practical protocol for utilizing FCS on primary lymphocytes was presented. In Paper III, we showed that NKp46 and Ly49A were confined within microdomains on NK cells. The actin cytoskeleton and cholesterol composition of NK cells played important roles in initiating activating cell signaling. In Paper IV, we investigated the organization and clustering of activating and inhibitory receptors on educated and uneducated NK cells. We found that clusters of NKp46 and Ly49A were larger on uneducated NK cells. The nearest neighbour distances from activating to inhibitory receptors were not significantly different between educated and uneducated NK cells, thus the organization of inhibitory receptors in relation to the activating receptors do not seem to be of importance for the educational process.In summary, the findings in this thesis enlightens the importance of altered receptor dynamics and organization on NK cells depending on the state of activation and education. Furthermore, receptor dynamics could be an important aspect for understanding NK cell function.List of scientific papersI. Cytokines Induce Faster Membrane Diffusion of MHC Class I and the Ly49A Receptor in a Subpopulation of Natural Killer Cells. Sunitha Bagawath-Singh, Elina Staaf, Arie Jan Stoppelenburg, Thiemo Spielmann, Taku Kambayashi, Jerker Widengren and Sofia Johansson. Front Immunol. Feb 4;7:16. 2016. https://doi.org/10.3389/fimmu.2016.00016 II. Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy. Elina Staaf, Sunitha Bagawath-Singh, Sofia Johansson. J Vis Exp. 1;(120) 2017. https://doi.org/10.3791/54756 III. Educated Natural Killer Cells Exhibit a More Dynamic Movement of NKp46 and a Higher Confinement of the Ly49A Receptor. E. Staaf, P. N. Hedde, Sunitha-Bagawath Singh, J. Piguet, E. Gratton, S. Johansson. [Submitted]IV. Nano-structural Organization of Activating and Inhibitory Receptors in Murine Natural Killer cell Education. Sunitha Bagawath Singh, Jan Bergstrand, Daniel Rönnlund, Hans Blom, Jerker Widengren and Sofia Johansson. [Manuscript]</p
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