850 research outputs found
Genetic bases for the metabolism of the DMS precursor S-methylmethionine by Saccharomyces cerevisiae
No full textDimethyl sulfide (DMS) is a sulfur containing volatile that enhances general fruity aroma and imparts aromatic notes in wine. The most important precursor of DMS is S-methylmethionine (SMM), which is synthesized by grapes and can be metabolized by the yeast S. cerevisiae during wine fermentation. Precursor molecules left after fermentation are chemically converted to DMS during wine maturation, meaning that wine DMS levels are determined by the amount of remaining precursors at bottling. To elucidate SMM metabolism in yeast we performed quantitative trait locus (QTL) mapping using a population of 130 F2-segregants obtained from a cross between two wine yeast strains, and we detected one major QTL explaining almost 30% of trait variation. Within the QTL, gene YLL058W and SMM transporter gene MMP1 were found to influence SMM metabolism, from which MMP1 has the bigger impact. We identified and characterized a variant coding for a truncated transporter with superior SMM preserving attributes. A population analysis with 85 yeast strains from different origins revealed a significant association of the variant to flor strains and minor occurrence in cheese and wine strains. These results will help selecting and improving S. cerevisiae strains for the production of wine and other fermented foods containing DMS such as cheese or beer.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.BT/Industriele Microbiologi
Use of DMS for paperless office
No full textTématem této bakalářské práce je využití DMS pro bezpapírovou kancelář. V první části se autor zabývá tím, co je potřeba brát v úvahu při realizaci bezpapírové kanceláře. Jaké legislativní aspekty jsou spojené s provozem bezpapírové kanceláře v České republice a jaké zázemí je k tomu potřeba. Autor při psaní této bakalářské práce spolupracoval se společností AiP Safe s.r.o., která mu poskytla analýzy projektů nasazení DMS. Z těchto analýz je poté zpracována druhá část, kde je popsáno, na které agendy je systém SAFE společnosti AiP Safe s.r.o. nejčastěji nasazován. Postupně je u každé agendy popsán výchozí stav, řešení v systému SAFE a shrnutí přínosů bezpapírové kanceláře. Závěr práce je věnován vyhodnocení, jestli nasazením vhodného DMS roste rychlost a efektivita procesů a činností, a zda dochází k dodržování legislativy a interních pravidel.The theme of this bachelor thesis is the use of DMS for paperless office. In the first part the author deals with what must be taken into account when realization a paperless office. What are the legal aspects associated with operating a paperless office in the Czech Republic and what facility is needed to do. When author writing this bachelor thesis, he has worked with AiP Safe s.r.o., which gave him the analysis of projects. From these analysis is then processed the second part, where is described which agenda is the most often deployed by system SAFE. Then author describes what the initial situation was and what benefits from the use of paperless office for various companies meant. Conclusion of the thesis is devoted to evaluation, if appropriate deployment of DMS increases speed and efficiency of processes and activities, whether there is compliance with legislation and internal rules
Use of DMS for paperless office
No full textThe theme of this bachelor thesis is the use of DMS for paperless office. In the first part the author deals with what must be taken into account when realization a paperless office. What are the legal aspects associated with operating a paperless office in the Czech Republic and what facility is needed to do. When author writing this bachelor thesis, he has worked with AiP Safe s.r.o., which gave him the analysis of projects. From these analysis is then processed the second part, where is described which agenda is the most often deployed by system SAFE. Then author describes what the initial situation was and what benefits from the use of paperless office for various companies meant. Conclusion of the thesis is devoted to evaluation, if appropriate deployment of DMS increases speed and efficiency of processes and activities, whether there is compliance with legislation and internal rules
DMS pilot implementation in banking environment
No full textThe diploma thesis focuses on the analysis of a document management system (DMS) pilot implementation in the environment of a Czech bank. The thesis is divided into three parts; the first part is theoretical and remaining two parts are practical (analytical). The theoretical part is devoted to summarization of basic theoretical knowledge in the field of DMS and in a pilot deployment of information systems. The second part describes the EMC xCP 2.1 platform and evaluates its advantages and disadvantages compared to competing products. The third part describes the technological implementation and deployment of a new DMS pilot solution in a bank. Further it analyzes and describes the follow-up activities and related projects that increase the DMS usability in a real business applications. The diploma thesis concurrently focuses on the business requirements analysis including constrains of a new solution deployment, as well as the DMS technological aspects description and the solution design. The author of this thesis has been working as an analyst in the IT company and has been in a long-term cooperation with the DMS team in the bank. The motivation for choosing this topic is to make a summary and clarification of knowledge gained on projects in the the bank and to take an advantage of it in the future cooperation
DMS pilot implementation in banking environment
No full textDiplomová práce je zaměřena na analýzu procesu pilotního zavádění systému na správu dokumentů (DMS) v prostředí české banky. Práce je rozdělena na tři části, přičemž první část je teoretická a zbývající dvě části jsou praktické (analytické). První část shrnuje základní teoretické poznatky v oblasti systémů pro správu dokumentů a pilotního zavádění informačních systémů. V druhé části je popsána platforma EMC Documentum xCP 2.1 a jsou zhodnoceny její výhody a nevýhody oproti konkurenčním produktům. Ve třetí části je popsán proces technologického nasazení a implementace nového DMS řešení v rámci pilotu v bance. Dále jsou v práci analyzovány a popsány navazující činnosti a související projekty, rozšiřující využitelnost DMS v reálných business agendách. Práce je zaměřena jak na popis technologických aspektů DMS, tak i na analýzu a popis hlavních business požadavků a problémů souvisejících se zaváděním nového řešení. Autor práce je analytikem v dodavatelské IT společnosti a dlouhodobě spolupracuje s DMS týmem v dané bance. Motivem k výběru tématu je shrnutí a ujasnění poznatků získaných při práci na projektech v bance a jejich využití pro budoucí spolupráci.The diploma thesis focuses on the analysis of a document management system (DMS) pilot implementation in the environment of a Czech bank. The thesis is divided into three parts; the first part is theoretical and remaining two parts are practical (analytical). The theoretical part is devoted to summarization of basic theoretical knowledge in the field of DMS and in a pilot deployment of information systems. The second part describes the EMC xCP 2.1 platform and evaluates its advantages and disadvantages compared to competing products. The third part describes the technological implementation and deployment of a new DMS pilot solution in a bank. Further it analyzes and describes the follow-up activities and related projects that increase the DMS usability in a real business applications. The diploma thesis concurrently focuses on the business requirements analysis including constrains of a new solution deployment, as well as the DMS technological aspects description and the solution design. The author of this thesis has been working as an analyst in the IT company and has been in a long-term cooperation with the DMS team in the bank. The motivation for choosing this topic is to make a summary and clarification of knowledge gained on projects in the the bank and to take an advantage of it in the future cooperation
DMSP and DMS in coastal fast ice and under-ice water of Lützow-Holm Bay, eastern Antarctica
No full textThe combined concentration of total dimethylsulfoniopropionate and dimethylsulfide (DMSP+DMS) were measured in Antarctic fast ice on the coast of Lützow-Holm Bay, eastern Antarctica. High bulk-ice DMSP+DMS and chlorophyll a concentrations were found at the bottom of the sea ice, and these concentrations were higher than those in the under-ice water. The bulk-ice DMSP+DMS and chlorophyll a concentrations were highly correlated (r2=0.68, P<0.001), suggesting that the high bulk-ice DMSP+DMS concentrations were caused mainly by the presence of algae assemblages in the ice. The calculated brine DMSP+DMS concentrations were as high as 1100 nM in the bottom ice layer, and the vertical profile patterns of brine DMSP+DMS concentrations were almost the same as for the bulk ice, mainly because of the small amount of variability in the vertical brine volume fraction. DMSP+DMS and chlorophyll a concentrations in the under-ice water increased, whereas the salinity of the under-ice water decreased, during the sampling period. These results reflect the supply of freshwater containing high levels of DMSP+DMS to the water just under the ice as the ice melted. These results suggest that sea-ice melting could be important to sulfur cycling in coastal ice-covered regions of the polar oceans
DMS pilot implementation in banking environment
No full textDiplomová práce je zaměřena na analýzu procesu pilotního zavádění systému na správu dokumentů (DMS) v prostředí české banky. Práce je rozdělena na tři části, přičemž první část je teoretická a zbývající dvě části jsou praktické (analytické). První část shrnuje základní teoretické poznatky v oblasti systémů pro správu dokumentů a pilotního zavádění informačních systémů. V druhé části je popsána platforma EMC Documentum xCP 2.1 a jsou zhodnoceny její výhody a nevýhody oproti konkurenčním produktům. Ve třetí části je popsán proces technologického nasazení a implementace nového DMS řešení v rámci pilotu v bance. Dále jsou v práci analyzovány a popsány navazující činnosti a související projekty, rozšiřující využitelnost DMS v reálných business agendách. Práce je zaměřena jak na popis technologických aspektů DMS, tak i na analýzu a popis hlavních business požadavků a problémů souvisejících se zaváděním nového řešení. Autor práce je analytikem v dodavatelské IT společnosti a dlouhodobě spolupracuje s DMS týmem v dané bance. Motivem k výběru tématu je shrnutí a ujasnění poznatků získaných při práci na projektech v bance a jejich využití pro budoucí spolupráci.The diploma thesis focuses on the analysis of a document management system (DMS) pilot implementation in the environment of a Czech bank. The thesis is divided into three parts; the first part is theoretical and remaining two parts are practical (analytical). The theoretical part is devoted to summarization of basic theoretical knowledge in the field of DMS and in a pilot deployment of information systems. The second part describes the EMC xCP 2.1 platform and evaluates its advantages and disadvantages compared to competing products. The third part describes the technological implementation and deployment of a new DMS pilot solution in a bank. Further it analyzes and describes the follow-up activities and related projects that increase the DMS usability in a real business applications. The diploma thesis concurrently focuses on the business requirements analysis including constrains of a new solution deployment, as well as the DMS technological aspects description and the solution design. The author of this thesis has been working as an analyst in the IT company and has been in a long-term cooperation with the DMS team in the bank. The motivation for choosing this topic is to make a summary and clarification of knowledge gained on projects in the the bank and to take an advantage of it in the future cooperation
Development, implementation and applications of CaptiveSpray differential ion mobility spectrometry (DMS) mass spectrometry for mixture analysis
No full textPh.D.Differential ion mobility spectrometry (DMS) spatially separates ions in the gas phase using the mobility differences of the ion between low and high electric field. The use of DMS as an ion filter (or ion selector) prior to mass spectrometry (MS) analysis has been compromised by the limited ion transmission efficiency. This thesis reports enhancement of the DMS-MS sensitivity and signal stability using a modified CaptiveSpray™ source. In terms of the ion sampling and transmission efficiency, the modified CaptiveSpray source swept ~ 89% of the ions generated by the tapered capillary through the DMS device (compared to ~ 10% with a conventional microspray source). The signal fluctuation improved from 11.7% (relative standard deviation, RSD) with microspray DMS-MS to 3.6% using CaptiveSpray-DMS-MS. A computational fluid dynamics (CFD) analysis was conducted to investigate the effect of carrier gases on signal intensity and peak width. Coupling of LC to DMS-MS via the modified CaptiveSpray source was simple and robust. Using DMS as a noise-filtering device, LC-DMS-MS performed better than conventional LC-MS for analyzing a BSA digest standard. Although LC-DMS-MS had a lower sequence coverage (55%), a higher Mascot score (283) was obtained compared to those of LC-MS (sequence coverage: 65%; Mascot score: 192) under the same elution conditions. The improvement in the confidence of the search result was attributed to the preferential elimination of noise ions.Using DMS as an ion separation device, an effective separation strategy for mixture analysis was introduced, involving the use of stepwise changes in the gas modifier concentration. Groups of bioactive ginsenoside/pseudo-ginsenoside isomers, including Rf/Rg₁/F₁₁, Rb₂/Rb₃/Rc, and Rd/Re, in the ginseng extracts were sequentially separated. The identities of the spatially separated ginsenoside/pseudo-ginsenoside isomers were confirmed by their characteristic compensation voltages(CoV) at specific modifier loading and MS/MS product ions. The origin of the whole and sliced ginseng could be confirmed either based on diagnostic chemical components or using quantitative ratios of three sets of ginsenoside markers, namely, Rg₁/Re, Rb₁/Rg₁, and Rb₂/Rc. This separation strategy was also used to separate two pairs of epimeric glycopeptides (EIDYIT(Man)PAR/EIDYIT(Glc)PAR and EIDYIT(Gal)PAR/EIDYIT(Glc)PAR) prior to MS analysis.Finally, this thesis also reports a CaptiveSpray tandem differential ion mobility spectrometry (DMS/DMS) method coupled with mass spectrometry for analysis of isomeric compounds, protein mixtures and tryptic digest of protein samples. The effect of asymmetric field strength with identical or different dispersion voltage in two sequential channel of DMS/DMS was experimentally evaluated by isomeric ginsenosides separation. Modulation of chemical composition of carrier gas result in differential ion molecule interaction in DMS/DMS. In a mixture of ethyl acetate and 1-propanol as gas modifier, a competition between two gas modifier molecules and ions was experimentally observed and ethyl acetate showed higher binding affinity compared with 1-propanol. In a mixture of cytochrome c, ubiquitin, insulin and melittin, a DMS/DMS separation was presented. Smaller proteins were found to have higher compensation voltage (CoV). In the last experiment, a grounded channel was connected before the DMS/DMS channel and showed significant reduction of the FWHM of the ionograms in both DMS-1 and DMS-2 dimensions (i.e. sharping the contour plot), meanwhile sacrificing 20%-50% of signal intensity.差分離子遷移譜（DMS）根據高、低電場中離子遷移率有差異的性質，分離氣相中的離子。作為質譜分析方法前的離子過濾（或離子選擇）方法，差分離子遷移譜的應用仍局限於其較低的離子傳輸效率。本論文展示了一種將改進捕獲噴霧（CaptiveSpray™）離子源與DMS串聯使用、以提高差分離子遷移譜-質譜（DMS-MS）技術信號強度和穩定性的方法。以離子採樣和傳輸效率為例，錐形毛細管產生的離子在通過差分離子遷移譜裝置之後，改進後的CaptiveSpray™離子傳輸效率為89% （傳統電噴霧離子源，microspray的傳導效率約為10%）。利用CaptiveSpray DMS-MS，信號波動從microspray DMS-MS的11.7% (RSD) 提高到3.6% (RSD)。利用計算流體動力學（CFD）方法分析了載氣對信號強度和信號峰峰寬的影響。改進後的CaptiveSpray離子源與液相色譜（LC）、DMS-MS聯用是便捷且可靠的。利用DMS過濾干擾信號後，LC-DMS-MS在分析牛血清蛋白消解樣品時的表現優於傳統的LC-MS方法。在相同洗脫條件下，與LC-MS相比（測序覆蓋度65%； Mascot score：192），LC-DMS-MS的測序覆蓋度（55%）儘管較低，但能取得更高的Mascot score（283）。搜索可信度結果的提高是歸功於DMS對雜質離子的選擇性去除。利用DMS作為一種氣相離子的分離設備，本論文介紹了一種漸進地改變氣體修飾劑（gas modifier）濃度高效分離混合物質的方法。幾組人參萃取物中具有生物活性的人參皂苷/擬人參皂苷同分異構體被依次分離，包括Rf/Rg₁/F₁₁，Rb₂/Rb₃/Rc和Rd/Re。分離後的人參皂苷/擬人參皂苷同分異構體根據特定的補償電壓（CoV）和MS/MS碎片離子進行確認。人參和人參片的種類根據診斷性的化學成分或三組人參皂苷標記物的定量比值，包括Rg₁/Re，Rb₁/Rg₁和Rb₂/Rc。另外，這一分離方法亦用於質譜方法前分離兩對差向異構糖肽（EIDYIT(Man)PAR/EIDYIT(Glc)PAR和 EIDYIT(Gal)PAR/EIDYIT(Glc)PAR）。在本論文的最後章節展示了一種二級差分離子遷移譜與質譜聯用方法（DMS/DMS-MS），並分析同分異構體、蛋白質混合物和胰蛋白酶消解蛋白質樣品。通過人參皂苷同分異構體的分離，說明了 DMS/DMS中，兩個順序連接的DMS使用相同或不同的不對稱電場強度對分析性能的影響。在DMS/DMS中，改變載氣的化學組分導致了有差異的離子-分子相互作用 。利用乙酸乙酯和正丙醇作為氣體修飾劑，發現兩者與離子的競爭性相互作用，乙酸乙酯與離子之間的結合親和力更強。利用DMS/DMS分離細胞色素c，泛素，胰島素和蜂毒蛋白質混合物，發現更小分子量的蛋白質具有更大補償電壓。最後，grounded channel被連接在DMS/DMS前，雖然損失20%-50%信號強度，但明顯的降低二維譜圖的半峰寬。Wu, Ri."October 2019."Thesis Ph.D. Chinese University of Hong Kong 2020.Includes bibliographical references (leaves 139-149).Abstracts also in Chinese.Title from PDF title page (viewed on 12, April 2021)
weileiw/ANN-DMS-code: Neural Network regression model to predict DMS monthly climatology
No full textThe ANN models are based on the TensorFlow Keras framework
There are five directories, Main, BinnedModel, CrossVali, PostProcessing, and linear-multilinear
The main directory contains functions that are used in the paper. parm_signif_tests.py is to test parameter combinations and rank the models according to their predictive ability. Results are used to produce Fig.2a.
ANN_DMS_fine_tune.py is to fine-tune the models. addAttribs.py is to convert coordinate and data/time parameters and to add MLD normalized PAR. my_dms_model.py is to build a sequential ANN model.
BinnedModel directory contains functions to test models using binned data.
CrossVali directory contains functions to test n-fold cross-validation models.
linear_multilear contains functions to test linear and multilinear models. credits go to Martí Galí at Earth Sciences Department, Barcelona Supercomputing Center, 08034 Barcelona, Spain for functions dms_sd02.m, dms_vs07.m, and dms_sat.m
PostProcessing contains functions to post-process the model results.
.csv and .mat data used to train the network can be found in the following repo.: https://zenodo.org/record/3833233#.XsM4cBP0nV4
author: Wei-Lei Wang on May 18, 2020
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