4,141 research outputs found
Emotional availability (EA) teleintervention for adoptive families
No full text2012 Fall.Includes bibliographical references.This study evaluated the new online Emotional Availability (EA) Intervention for use with adoptive families in enhancing parent-child EA, parental perceptions of EA, child attachment behaviors, parent-child emotional attachment, and reducing parent-reported child behavioral problems and parenting-related stress. Participants in this study were adoptive parents and their adopted children ages 1.5 - 5 years old (N = 15 dyads). Participants were placed in an immediate intervention group (IG) or a delayed intervention group (DG) that would receive the 6-week EA Intervention after the IG. Results revealed significant differences in the IG in child behavioral problems, parent-child EA, parental perceptions of EA, and parent-child emotional attachment, improvements not seen in the DG. Analysis of effects of the DG after receiving the EA Intervention revealed significant differences over time also in child behavioral problems, parent-child EA, parental perceptions of EA, and parent-child emotional attachment. Implications, limitations, and future directions are discussed
Functional mapping of a trypanosome centromere by chromosome fragmentation identifies a 16-kb GC-rich transcriptional "strand-switch" domain as a major feature.
No full textTrypanosomatids are an ancient family that diverged from the main eukaryotic lineage early in evolution, which display several unique features of gene organization and expression. Although genome sequencing is now complete, the nature of centromeres in these and other parasitic protozoa has not been resolved. Here, we report the functional mapping of a centromere in the American trypanosome, Trypanosoma cruzi, a parasite with an unusual mechanism of genetic exchange that involves the generation of aneuploidy by nuclear hybridization. Using a telomere-associated chromosome fragmentation approach, we show that the region required for the mitotic stability of chromosome 3 encompasses a transcriptional "strand-switch" domain constituted by a 16-kb GC-rich island. The domain contains several degenerate retrotransposon-like insertions, but atypically, lacks the arrays of satellite repeats normally associated with centromeric regions. This unusual type of organization may represent a paradigm for centromeres in T. cruzi and other primitive eukaryotes
Environmental (waste) compliance control systems for UK SMEs
No full textWhile the ‘environment’ is often perceived as a heavily regulated area of business, in reality, directly-regulated businesses represent a small proportion of the business community. This study aimed to evaluate and outline potential improvements to compliance controls for small and medium-sized enterprises (SMEs), particularly those involved in the waste sector. Forty-four SMEs from England were interviewed/audited between April-September 2008. Using a UK-based system as a case-in-point, the Environment Agency’s (EA) Operational Risk Appraisal (‘Opra’)/Compliance Assessment Report (CAR) system was analysed. Environmental compliance performance indicators and an initial assessment methodology for SMEs were developed. The study showed:• Compliance with permitting legislation was poor in many areas.• Regulatory authorities are either unable/failing to implement their enforcement policies or unable/failing to identify non-compliances due to the infrequency or limited nature of their inspections.• Improvements are needed to the EA Opra/CAR system – control measures are not fully taken into account when calculating risk.Recommendations to improve SME compliance controls include using internationally applicable general and specific compliance and non-compliance performance indicators, re-designing the Opra system and using an initial assessment methodology based on understanding the hazardousness of SME categories, compliance levels and operator competency.<br/
Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in <it>Clostridium acetobutylicum </it>EA 2018
No full textAbstract Background Clostridium acetobutylicum, a gram-positive and spore-forming anaerobe, is a major strain for the fermentative production of acetone, butanol and ethanol. But a previously isolated hyper-butanol producing strain C. acetobutylicum EA 2018 does not produce spores and has greater capability of solvent production, especially for butanol, than the type strain C. acetobutylicum ATCC 824. Results Complete genome of C. acetobutylicum EA 2018 was sequenced using Roche 454 pyrosequencing. Genomic comparison with ATCC 824 identified many variations which may contribute to the hyper-butanol producing characteristics in the EA 2018 strain, including a total of 46 deletion sites and 26 insertion sites. In addition, transcriptomic profiling of gene expression in EA 2018 relative to that of ATCC824 revealed expression-level changes of several key genes related to solvent formation. For example, spo0A and adhEII have higher expression level, and most of the acid formation related genes have lower expression level in EA 2018. Interestingly, the results also showed that the variation in CEA_G2622 (CAC2613 in ATCC 824), a putative transcriptional regulator involved in xylose utilization, might accelerate utilization of substrate xylose. Conclusions Comparative analysis of C. acetobutylicum hyper-butanol producing strain EA 2018 and type strain ATCC 824 at both genomic and transcriptomic levels, for the first time, provides molecular-level understanding of non-sporulation, higher solvent production and enhanced xylose utilization in the mutant EA 2018. The information could be valuable for further genetic modification of C. acetobutylicum for more effective butanol production.</p
Navigating Enterprise Architecture (EA) Institutionalization: The Interplay of EA and Agile
No full textIn the rapidly evolving context of digital transformation, the effective adoption, management, and institutionalization of Enterprise Architecture (EA) have emerged as significant efforts for organizations looking for ways to navigate the complex requirements of modern business environments. This Ph.D. thesis embarks on a comprehensive exploration of different aspects of EA, ranging from its adoption challenges to its interaction with agile transformations. This study's main objective is to shed light on different aspects of EA adoption, management, challenges, and integration within large-scale organizations.
The research questions that form the foundation of this thesis are designed to explore the complex nuances of an EA's journey within an organization. The thesis investigates the role of stakeholders in influencing the perception and institutionalization of EA in order to respond to the first research question. It highlights the complex interactions between different stakeholders and their different interests, showing how these interactions can have a big impact on how successful EA initiatives are. The study goes deeper into the organizational factors that contributed to the failure of EA management initiatives, explaining the significance of legitimacy and showing challenges to achieving normative and cultural-cognitive legitimacy.
The third research question focuses on the topic of delegitimization of established EA practices. The thesis shows the ways through which EA practices can lose momentum and relevance within organizations by examining the complex interplay of regulatory, pragmatic, normative, and cultural-cognitive legitimacy. Building on this foundation, the thesis navigates the cycles of EA's institutionalization, de-institutionalization, and re-institutionalization within the organizational context. This dynamic approach highlights the importance of regaining legitimacy and adapting to modern organizational approaches in order to achieve successful re-institutionalization.
In light of the growing popularity of agile approaches, the thesis investigates the challenges posed by such paradigm shifts to established EA practices. The study outlines the modifications that must be made to EA practices by identifying the main functions that EA must do to support organizational agility. The outcome of this study is a collection of five papers, each of which shows a different part of EA's journey. Together, these contributions enhance the understanding of EA's adoption, management, and integration with agile paradigms, significantly advancing the body of knowledge in this domain.
This Ph.D. thesis gives a comprehensive overview of EA's strategic, operational, and innovative capacity. It shows how stakeholders, organizational dynamics, and an evolving digital environment all work together. This thesis helps organizations looking to use the advantages of EA in their goals of sustainable growth and agility by addressing the research questions and highlighting different aspects of the EA's role.Fulltext not availabl
Mouse strains with an active H2-Ea meiotic recombination hot spot exhibit increased levels of H2-Ea-specific DNA breaks in testicular germ cells.
No full textWe devised a sensitive method for the site-specific detection of rare meiotic DNA strand breaks in germ cell-enriched testicular cell populations from mice that possess or lack an active recombination hot spot at the H2-Ea gene. Using germ cells from adult animals, we found an excellent correlation between the frequency of DNA breaks in the 418-bp H2-Ea hot spot and crossover activity. The temporal appearance of DNA breaks was also studied in 7- to 18-day-old mice with an active hot spot during the first waves of spermatogenesis. The number of DNA breaks detected rose as leptotene and zygotene spermatocytes populate the testis with a peak at day 14 postpartum, when leptotene, zygotene, and early pachytene spermatocytes are the most common meiotic prophase I cell types. The number of DNA breaks drops precipitously 1 day later, when middle to late pachytene spermatocytes become the dominant subtype. The recombination-related breaks in the hot spot likely reflect SPO11-induced double-strand breaks and/or recombination intermediates containing free 3\u27 hydroxyl groups
Genesis of ancestral haplotypes: RNA modifications and reverse transcription–mediated polymorphisms
No full textUnderstanding the genesis of the block haplotype structure of the genome is a major challenge. With the completion of the sequencing of the Human Genome and the initiation of the HapMap project the concept that the chromosomes of the mammalian genome are a mosaic, or patchwork, of conserved extended block haplotype sequences is now accepted by the mainstream genomics research community. Ancestral Haplotypes (AHs) can be viewed as a recombined string of smaller Polymorphic Frozen Blocks (PFBs). How have such variant extended DNA sequence tracts emerged in evolution? Here the relevant literature on the problem is reviewed from various fields of molecular and cell biology particularly molecular immunology and comparative and functional genomics. Based on our synthesis we then advance a testable molecular and cellular model. A critical part of the analysis concerns the origin of the strand biased mutation signatures in the transcribed regions of the human and higher primate genome, A-to-G versus T-to-C (ratio ~1.5 fold) and C-to-T versus G-to-A (≥1.5 fold). A comparison and evaluation of the current state of the fields of immunoglobulin Somatic Hypermutation (SHM) and Transcription-Coupled DNA Repair focused on how mutations in newly synthesized RNA might be copied back to DNA thus accounting for some of the genome-wide strand biases (e.g., the A-to-G vs T-to-C component of the strand biased spectrum). We hypothesize that the genesis of PFBs and extended AHs occurs during mutagenic episodes in evolution (e.g., retroviral infections) and that many of the critical DNA sequence diversifying events occur first at the RNA level, e.g., recombination between RNA strings resulting in tandem and dispersed RNA duplications (retroduplications), RNA mutations via adenosine-to-inosine pre-mRNA editing events as well as error prone RNA synthesis. These are then copied back into DNA by a cellular reverse transcription process (also likely to be error-prone) that we have called "reverse transcription-mediated long DNA conversion." Finally we suggest that all these activities and others can be envisaged as being brought physically under the umbrella of special sites in the nucleus involved in transcription known as "transcription factories."
[Ea-hei-no-maue, North Island, New Zealand] [cartographic material].
No full textFrom David Collins's Account of the English colony in New South Wales. London : Printed for T. Cadell jun. & W. Davies, in the Strand, 1798. Vol. 1.; Map of various Maori peoples in North Island, New Zealand, with illustrations of ships Daedalus and Britannia.; Ferguson, J.A. Bibliography of Australia, 263; Rex Nan Kivell Collection Map NK 11077
Visual aptamer-based capillary assay for ethanolamine using magnetic particles and strand displacement
No full textArt. 690, 8 S.This work describes an aptamer-based capillary assay for ethanolamine (EA). It is making use of strand displacement format and magnetic particles. The capillary tubes are coated with three layers, viz. (a) first with short oligonucleotides complementary to the aptamer (EA-comp.); (b) then with magnetic particles (Dynabeads) coated with EA-binding aptamer (EA-aptamer), and (c) with short oligonucleotide-coated magnetic particles (EA-comp.). On exposure to a sample containing ethanolamine, the DNA-coated magnetic particles are released and subsequently collected and spatially separated using a permanent magnet. This results in the formation of a characteristic black/brown spots. The assay has a visual limit of detection of 5 nM and only requires 5 min of incubation. Quantification is possible through capture and analysis of digital (RGB) photos in the 5 to 75 nM EA concentration range. Furthermore, results from tap water and serum spiked with EA samples showed that the platform performs well in complex samples and can be applied to real sample analysis. The combined use of plastic capillaries, visual detection and passive flow make the method suited for implementation into a point-of-care device.18
Emotional availability of adult interpersonal relationships questionnaire (EA-AIRQ): validation and implications for research and practice
No full text2017 Summer.Includes bibliographical references.Emotional Availability (EA) is commonly known as a parent-child construct used to describe the level of healthy emotional connection in the dyadic relationships (Biringen et al., 2014). Stemming from John Bowlby's (Bowlby, 1969) work on attachment, EA provides a gauge to the level of parent's receptiveness to a child's emotional feedback, both positive and negative (Biringen et al., 2014). In addition to relationships between the parent and child, EA conceptually should be applicable to a wide array of relationships. This paper will define the construct of EA and its foundations in attachment theory. It will then focus on the development and validation of a brief EA Adult Interpersonal Relationships Questionnaire (EA-AIRQ). A total of 215 Amazon Mechanical Turk workers were administered this measure (with request for participants to complete the measure for 'friends' and then for 'romantic partners'). Participants also completed the measures of attachment and mental well being. The EA-AIRQ was composited in two ways: 1-unit-weighted, with each item equally weighted (by adding all items), and 2-regression-weighted, that is, from a factor analysis for friends and romantic partners separately with the aim of obtaining a one-factor solution. Correlations between these composites and the other administered measures revealed meaningful patterns. Implications for research and clinical practice are discussed
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