7,527 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
No full textThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Stephens, Lois
No full textVester Stephens - husbandhttps://stars.library.ucf.edu/cfm-ch-memoranda-1927/1423/thumbnail.jp
Stephens, Berry M.
No full textZora Stephens - wifehttps://stars.library.ucf.edu/cfm-ch-memoranda-1927/1019/thumbnail.jp
THE IMPACT OF GENETIC HEARING IMPAIRMENT
No full textList of contributors -- Preface -- Acknowledgements -- Ch. 1. Future perfect: social aspects of genetics and deafness / Lesley Jones -- Ch. 2. Parents' attitudes towards genetic testing and the impact of deafness in the family / Anna Middleton -- Ch. 3. The International classification of functioning, disability and health as a conceptual framework for the impact of genetic hearing impairment / Dafydd Stephens and Berth Danermark -- Ch. 4. A common methodology for reviewing the impact of hearing impairment / Berth Danermark, Sophia Kramer and Dafydd Stephens -- Ch. 5. The impact of hearing impairment in children / Dafydd Stephens -- Ch. 6. A review of the psychosocial effects of hearing impairment in the working-age population / Berth Danermark -- Ch. 7. The psychosocial impact of hearing loss among elderly people: a review / Sophia Kramer -- Ch. 8. The impact of combined vision and hearing impairment and of deafblindness / Kerstin Möller -- Ch. 9. The effects of otosclerosis / Nele Lemkens -- Ch. 10. Psychosocial aspects of neurofibromatosis type 2 / Wanda Neary, Richard Ramsden, Gareth Evans and Michael Baser -- Ch. 11. Moving forward: a life of changes / Patricia Lago-Avery -- Ch. 12. My genetic deafness / Jill Jones -- Glossary -- Inde
Stephens, Martha A.
No full textA. C. Stephens - husbandhttps://stars.library.ucf.edu/cfm-ch-memoranda-1923/1131/thumbnail.jp
Stephens, Andrew Berry
No full textNora Lee Stephens - wifehttps://stars.library.ucf.edu/cfm-ch-memoranda-1940/1260/thumbnail.jp
Ongoing development of a semi-quantitative protocol for assessing the suitability of commercial materials used to store or exhibit cellulose-based artworks
No full textEmission of harmful volatile organic compounds (VOCs) from construction materials used to store or display artworks is a significant concern for cultural heritage stewards. In this study, a simple analytical protocol that evaluates the effect of off-gassed VOCs from construction materials on cellulose was developed. The study involved artificially aging Whatman (WT1) paper, a cellulose sensor which acted as a surrogate for cellulose-based artifacts in collections, in a sealed jar with nine commercially available construction materials at different aging conditions (60-80 degrees C for 14-28 days) to identify a viable aging protocol. High-pressure anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) measured the glucose produced during WT1 hydrolysis from water extracts of aged samples. Ultraviolet-visible (UV-Vis) diffuse reflectance spectroscopy non-invasively tracked changes in absorption in the 250-500 nm range. Tests showed 80 degrees C for 14 days to be the aging conditions to induce measurable degradation of the cellulose sensor when aged with construction materials. HPAEC-PAD and UV-Vis data were compared with two established paper degradation analytical methods, size exclusion chromatography (SEC) and carbonyl content measurements, as well as to a diagnostic VOCs protocol, the Oddy test. HPAEC-PAD identified glucose before changes in molecular weight were identified via SEC, and UV-absorbance only moderately correlated with increasing carbonyl content. While additional tests are necessary prior the adoption of this protocol, results to date indicate the potential for the approach as a more rapid and unbiased alternative to the Oddy test for evaluating construction materials to be used near cellulosic collections
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CACHD1: a new activity-modifying protein for voltage-gated calcium channels
No full textAutocommentary to: Cottrell GS, Soubrane CH, Hounshell JA, Lin H, Owenson V, Rigby M, Cox PJ, Barker BS, Ottolini M, Ince S, Bauer, CC, Perez-Reyes E, Patel MK, Stevens EB, Stephens GJ (2018) CACHD1 is an 2-like protein that modulates CaV3 voltage-gated calcium channel activity J Neurosci 38:9186-9201
Les diminutifs basques avec ch
No full textSe presentan formaciones similares a los diminutivos vascos con "ch" en España y América latina. Se dan ejemplosThe author introduces similar formations to the Basque diminutive "ch" in Spain and Latin America. Examples are provide
COUPLING OF THE C-H STRETCH TO LARGE-AMPLITUDE TORSION AND INVERSION MOTIONS: COMPARISON OF CHCH, CHOH AND CHNH
No full textAuthor Institution: Department of Polymer Science and Department of Chemistry, The University of Akron; Department of Chemistry, The University of Akron, OH 44325In each of the title molecules, torsional and inversion tunneling occurs between six equivalent minima. Coupling of these degrees of freedom to the CH stretch occurs via variation of the C-H stretching force constants as a function of the torsional () and inversion () angles. Maps of the couplings have been computed at the MP2/6-311++G(3df,2p) level. Both the single bond CH stretch force constants and the bilinear couplings between CH bonds are presented as a function of and . Although the torsional barriers differ by more than a factor of 20, the torsion-inversion-vibration coupling patterns are very similar for CHNH and CHCH. On the other hand, the torsion-inversion-vibration coupling in the charged species CHOH is much weaker
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