33 research outputs found
Enhancement of Cholinesterase Inhibition of Alpinia galanga (L.) Willd. Essential Oil by Microemulsions
No full textThis study aimed to investigate the chemical composition and reveal the selective inhibitory activity of Alpinia galanga (L.) Willd. essential oil (AGO) on acetylcholinesterase (AChE) compared to butyrylcholinesterase (BChE). The chemical composition of AGO was investigated by means of gas chromatography–mass spectrometry. Ellman’s method was used to determine the inhibitory activities against AChE and BChE. Microemulsion systems with desirable anticholinesterase effects were developed. Methyl cinnamate and 1,8-cineole were reported as the major component of AGO. The IC(50) values of A. galanga oil against AChE and BChE were 24.6 ± 9.6 and 825.4 ± 340.1 µg/mL, respectively. The superior selectivity of AGO on AChE (34.8 ± 8.9) compared to galantamine hydrobromide (6.4 ± 1.5) suggested AGO to be an effective ingredient with fewer side effects for Alzheimer’s treatment. Interestingly, the microemulsion of AGO possessed significantly higher anticholinesterase activity than that of native oil alone. Therefore, microemulsion of AGO is a promising alternative approach for the treatment of Alzheimer’s disease
Herbal Extracts as Potential Antioxidant, Anti‐Aging, Anti‐Inflammatory, and Whitening Cosmeceutical Ingredients
No full textOptimisation of stability and charge transferability of ferrocene-encapsulated carbon nanotubes
No full textTransforming Industrial Engineering Education: Introducing the CWILE Model for Work-Integrated Learning in the Digital Age
Get PDFThailand\u27s industrial sector has made significant progress in adopting and implementing technology associated with Industry 4.0. As technology advances and industries become more digitally driven, a skilled and adaptable workforce becomes paramount. The responsibility for cultivating such human resources lies with educational institutions. They are the foundation for developing a workforce that can thrive in the digital age by staying responsive to industry trends, fostering business collaboration, and prioritising technical and soft skills. Work-integrated learning (WIL) is one of the educational programs that can help improve student skills by integrating the theoretical exploration of a particular subject with its practical implementation within a professional environment. The WIL program enables individuals to demonstrate competence, expertise, and the capacity to keep up with contemporary technologies. This paper proposes the new WIL model for the WIL program in the industrial engineering profession, known as the CWILE model. The study aims to identify and establish the benefits of the CWILE model. These benefits include enhanced skill development, improved readiness for industry demands, and a seamless transition from education to the workforce. The initial step in the research procedure involved collecting stakeholders\u27 perspectives on the existing WIL program in the industrial engineering department of the engineering faculty at Rajamangala University of Technology Lanna (RMUTL), Thailand. Following stakeholder feedback, the CWILE model was developed and implemented with the participation of eighteen students in the WIL program. The effectiveness of the CWILE model was assessed through a comprehensive evaluation of student competencies: core competency, functional competency, and professional competency. The evaluations were conducted at three distinct intervals: before, during, and after instruction. This longitudinal assessment allows a nuanced understanding of how the WIL program influences student competencies. The results of this study show that the duration of attending the WIL program is positively correlated with an increase in all competencies
Study of iron and aluminum binding to Suwannee River fulvic acid using absorbance and fluorescence spectroscopy: Comparison of data interpretation based on NICA-Donnan and Stockholm humic models
No full textThis study examined the evolution of absorbance and fluorescence spectra of standard Suwannee River fulvic acid (SRFA) induced by its interactions with iron and aluminum. The results show that changes of SRFA absorbance are associated with a consistent response of the carboxylic and phenolic functional groups to iron and aluminum forming bonds with these groups, and their deprotonation induced by such binding. The observed changes of SRFA absorbance were quantified via the use of DSlope(325-375) parameter that determines the behavior of the slope of logarithms of SRFA absorbance in the range of wavelengths 325 -375 nm in the presence of varying concentrations of iron or aluminum. DSlope(325-375) values were correlated linearly with the concentration of SRFA-bound iron and aluminum determined using either NICA-Donnan or Stockholm Humic Model (SHM) but the correlation was stronger for the former model (R-2 > 0.98). The slopes of these correlations were similar for both iron and aluminum concentrations <10.0 mu M and at a wide pH range. Fluorescence of SRFA was responsive to metal binding but it changed less consistently in the presence of the examined metals, especially in the case of aluminum. The combination of these techniques can help explore in more detail manifestations of DOM site specificity at realistically low concentrations of DOM and metal ions. (C) 2013 Elsevier Ltd. All rights reserved.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000324566400059&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Engineering, EnvironmentalEnvironmental SciencesWater ResourcesSCI(E)EIPubMed16ARTICLE145439-54464
The Use of Vaccinium elliottii Chapmn. in Breeding Highbush Blueberry
No full textEighty vigorous V. elliottii seedlings were selected from an 8-km stretch of Perone Creek, in southwest Alabama near Silverhill. Four ramets of each plant were obtained by rooting softwood cuttings taken in August 2013. These were grown in pots of peat in a greenhouse until March 2014. They began to flower in early February, and on March 1 they were placed outside the greenhouse for open pollination. Nineteen tetraploid southern highbush selections, growing in 20-liter pots, which had been chilled in a cooler, were placed in a bee-proof greenhouse in February 2014. Flowers on these plants were emasculated before they opened, and approximately 200 flowers per plant were pollinated with pollen from one or more of the potted V. elliottii selections. From 4301 pollinated flowers, only 78 seeds judged to be germinable were obtained. By contrast, crosses between tetraploid highbush cultvars gave 6720 seeds from 349 pollinated flowers and crosses between two V. elliottii clones gave 8000 seeds from 380 flowers. The 80 V. elliottii clones placed outside for pollination received few or no bee visits during flowering, which continued during March and April, and fruit set was less than 1%. Mean weight of berries from several hundred V. elliottii plants sampled at five forested locations in northwest Florida and southwest Alabama ranged from 184 mg to 341 mg depending on the location. Two berry sample sites in Alabama were along Perone Creek, and were sites from which plants had been propagated for the crossing experiments. Two V. elliottii plants with fastigiate growth habit were found, one at Perone Creek and one along the Suwannee River in Hamilton County, Florida.Paper presented at NABREW Conference, Paper Session I:Blueberry Greeding, on June 24, 2014, Atlantic City, N.J
Assessment of Contamination Risk from Fecal Matter Presence on Fruit and Mulch in the tomato fields based on generic Escherichia coli population
No full textThe purpose of this study was to evaluate the fate of generic Escherichia coli in fecal pats under different field conditions and to predict Salmonella and Shiga toxin-producing E. coli (STEC) survival dynamics based on developed models. Eight trials were conducted during spring and fall in both North and Central Florida. Fresh cattle feces (1g) was placed on mature green round tomatoes located inside (TIP) and outside (TOP) of the plant canopy. Fecal pats (10 g) were placed under (MUP) and distant (MDP) to tomato plants on plastic mulch. Pathogen populations were predicted based on developed models. Declines in generic E. coli populations over 7 days (Log CFU/g) in fecal pats were between 0.9 and 2.7 on TIP, 1.2 and 3.0 on TOP, 0.2 and 1.2 on MUP, and 0.4 and 1.5 on MDP in the Central Florida fall and spring trials, respectively. E. coli populations remained stable at ?4.2 and ? 6.5 Log CFU/g during all North Florida trials. The concentration changes in predicted Salmonella and STEC population were less than 2.1 Log CFU/g in fecal pats for all conditions. Developed models predicted similar pathogen survival trends to generic E. coli with no dramatical impact under the field conditions. © 2021 Elsevier LtdU.S. Department of Agriculture, USDA: SCRI-2011-51181-30767; National Institute of Food and Agriculture, NIFA; Institute of Food and Agricultural Sciences, University of Florida, IFASThis material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Specialty Crops Research Initiative (SCRI), under awards SCRI-2011-51181-30767. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. The authors thank to the staff at the two different field sites at the University of Florida's Institute of Food and Agricultural Sciences (UF/IFAS) Gulf Coast Research and Education Center located in Wimauma, Florida (Central Florida) and the North Florida Suwannee Research and Education Center ? Suwannee Valley in Live Oak, Florida. The authors also thank Travis Chapin, Loretta Friedrich, Gwen Lundy, Luis Edgardo Martinez, and Gonca Buyruko?lu for technical support.This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture , Specialty Crops Research Initiative (SCRI), under awards SCRI-2011-51181-30767 . Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. The authors thank to the staff at the two different field sites at the University of Florida's Institute of Food and Agricultural Sciences (UF/IFAS) Gulf Coast Research and Education Center located in Wimauma, Florida (Central Florida) and the North Florida Suwannee Research and Education Center – Suwannee Valley in Live Oak, Florida. The authors also thank Travis Chapin, Loretta Friedrich, Gwen Lundy, Luis Edgardo Martinez, and Gonca Buyrukoğlu for technical support
Impact of instrument and experiment parameters on reproducibility of ultrahigh resolution ESI FT-ICR mass spectra of natural organic matter
No full textAuthor Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Organic Geochemistry 41 (2010): 725-733, doi:10.1016/j.orggeochem.2010.05.017.Natural dissolved organic matter (OM) is a complex heterogeneous mixture of compounds that
have defied traditional characterization using standard analytical methods. Electrospray
ionization mass spectrometry, particularly ultrahigh resolution mass spectrometry, has
provided a new platform for compositional assessment of this important pool of the Earth’s
reduced carbon. Here, we propose a framework for optimization of instrument and experiment
parameters for high quality data acquisition using Fourier‐transform ion cyclotron resonance
mass spectrometry (FT‐ICR‐MS). We report the impact of these parameters on reproducibility of
peak detection and repeatability of peak height in replicate injections of Suwannee River fulvic
acid (FA), a common terrestrial OM standard. In addition, we examine the variability in peak
detection and peak height among different types of experimental replicates of dissolved OM
derived from laboratory cultures of Candidatus Pelagibacter ubique, a ubiquitous marine α‐
proteobacterium.The National Science
Foundation (Major Research Instrumentation program: OCE‐0619608 (E.B.K.), the Chemical
Oceanography program: CAREER‐OCE‐0529101 (E.B.K.) and OCE‐0751897 (E.B.K. and S.J.G.)),
the Marine Microbiology Initiative at the Gordon and Betty Moore Foundation (S.J.G.) and
supporting funds from the WHOI Director of Research (E.B.K.)
Experimental studies of Hg(II)-Hg(0) transformations and their effects on Hg isotope fractionation
No full textUnderstanding the redox transformations of inorganic forms of mercury (Hg) is necessary for understanding the fate of mercury in environmental systems. In this study, the interactions of Hg(0) and Hg(II) with organic and inorganic substances were characterized using mercury stable isotopes. Interactions of a mixed Hg(0)-Hg(II) solution with thiol and humic substances were observed, with no net changes to redox speciation. In the presence of mercaptoacetic acid (MCA), an equilibrium isotope enrichment factor (ε202Hg = δ202HgHg(II) - δ202HgHg(0)) of 1.34‰ between the Hg(II) and Hg(0) fractions was observed, similar with previously published values for Hg(0) oxidation by thiols. The equilibrium ε202Hg values similarly determined for 2-mercaptopropionic acid (MPA) and Suwannee River humic acids were 2.03‰ and 1.50‰, respectively. Reduction of mercury by siderite (FeCO3) was also characterized with respect to isotope fractionation over the course of the reaction. This reaction resulted in an 87% reduction of Hg(II) over 30 minutes, with change in mercury isotope ratios of the reactant. Kinetic ε202Hg values for the reduction of Hg(II) by siderite (ε202Hg = δ202HgHg(0) - δ202HgHg(II)) were determined by closed-system model (-1.59‰) and Rayleigh distillation model (-1.07‰; product enrichment in light isotopes). The final equilibrium state exhibited an equilibrium ε202Hg of -0.67‰. The results from the experiments conducted suggest that there is equilibrium isotope exchange between thiol-bound Hg(II) and dissolved Hg(0), and that the reduction by siderite is rapid and may involve multiple processes through the reaction.M.S.Includes bibliographical reference
Molecular Insights into the Transformation of Dissolved Organic Matter in Landfill Leachate Concentrate during Biodegradation and Coagulation Processes Using ESI FT-ICR MS
No full textLandfill leachate concentrate is a type of refractory organic wastewater with high environmental risk. Identification of refractory components and insights into the molecular transformations of the organics are essential for the development of efficient treatment process. In this report, molecular compositions of dissolved organic matter (DOM) in leachate concentrate, as well as changes after anaerobic/aerobic biodegradation and coagulation with salts, were characterized using electrospray ionization (ESI) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). DOM in leachate concentrate were more saturated and less oxidized with more nitrogen and sulfur-containing substances (accounting for 50.0%), comparing with natural organic matter in Suwannee River. Selectivity for different classes of organics during biodegradation and coagulation processes was observed. Substances with low oxidation degree (O/C < 0.3) were more reactive during biodegradation process, leading to the formation of highly oxidized molecules (O/C > 0.5). Unsaturated (H/C < 1.0) and oxidized (O/C > 0.4) substances containing carboxyl groups were preferentially removed after coagulation with Al or Fe sulfate. The complementary functions of biodegradation and coagulation in the treatment of DOM in leachate concentrate were verified at the molecular level. Lignin-derived compounds and sulfur-containing substances in leachate concentrate were resistant to biodegradation and coagulation treatments. To treat leachate concentrate more effectively, processes aimed at removal of such DOM should be developed.National Natural Science Foundation of China [91434132, 51578006, 51378020]; Collaborative Innovation Center for Regional Environmental QualitySCI(E)ARTICLE148110-81185
