1,818 research outputs found
Interregno estadunidense. Entrevista de Nancy Fraser a Alessandra Spano
No full textO texto original “American Interregnum” foi publicado na New Left Review - Sidecar no dia 09 Abr. 2021, no sítio oficial da revista: .
Tradução de Nicole Herscovici
Effects of hydrogen peroxide on slow- and fast-growing NIH/3T3-derived cultures: nuclear and cytoplasmic aspects related to senescence and transformation
No full textCellular senescence can occur with similar phenotypes in normal cells, during aging, and in tumor cells, spontaneously or after cytostasis. The fall or increase in proliferative activity are key aspects of the respective conditions, in which the levels of reactive oxygen species can vary, affecting the cellular redox homeostasis. This work aimed to study the relationships between senescence and transformation by comparing cells with different proliferative activities and phenotypes attributable to transformation (NIHs cultures) or senescence (NIHv cultures), before and after incubation with hydrogen peroxide. Both cultures were derived from the NIH/3T3 cell line, which was used here as a reference (NIHb), after the serum starvation. Our experimental model can be representative of the heterogeneity of cell subpopulations, with different degrees of transformation and senescence, found in some tumors. The characterization of the functional properties of NIHb, NIHs, and NIHv cells was performed by a morphocytometric analysis of the cell cycle progression, mitochondrial and lysosomal content/activity, and superoxide anion production. The efficiency of the lysosomal compartment was also assessed by estimating the autophagic activity and measuring lipofuscin autofluorescence. Comparisons of nuclear and cytoplasmic parameters before and after the incubation with hydrogen peroxide revealed differences in the expression and modulation of cellular senescence patterns. The treatment effects were very limited in the NIHb culture; the senescence condition was essentially maintained in the NIHv cells, while the most relevant changes were found in the NIHs cells. In the latter, the acquisition of the senescent phenotype, also demonstrated by the positivity of SA-β-galactosidase, was correlated with a decrease in proliferative activity and a change in the content/activity of the mitochondria and lysosomes, which showed similarities with the basal senescence conditions of NIHv cells. In NIHs cells, increased autophagy events and lipofuscin accumulation also indicate the establishment of cytoplasmic dynamics typical of senescence. The variable responses to hydrogen peroxide, besides depending on the different basal cytokinetic activity of the cultures examined, appeared to be related to the specific cell redox state resulting from the balance between endogenous ROS and those produced after treatment. Especially in NIHs cells, the slowing down of the cell cycle was linked to dynamic interconnections between the mitochondrial and lysosomal compartments. This would indicate that transformed cells, such as NIHs, may express morpho-functional aspects and markers typical of cellular senescence, as a consequence of the modulation of their redox state
Polyploid cell dynamics and death before and after PEG-treatment of a NIH/3T3 derived culture: vinblastine effects on the regulation of cell subpopulations heterogeneity
No full textBackground: Neoplastic subpopulations can include polyploid cells that can be involved in tumor evolution and recurrence. Their origin can be traced back to the tumor microenvironment or chemotherapeutic treatment, which can alter cell division or favor cell fusion, generating multinucleated cells. Their progeny, frequently genetically unstable, can result in new aggressive and more resistant to chemotherapy subpopulations. In our work, we used NIHs cells, previously derived from the NIH/3T3 line after serum deprivation, that induced a polyploidization increase with the appearance of cells with DNA content ranging from 4 to 24c. This study aimed to analyze the cellular dynamics of NIHs culture subpopulations before and after treatment with the fusogenic agent polyethylene glycol (PEG), which allowed us to obtain new giant polyploid cells. Successively, PEG-untreated and PEG-treated cultures were incubated with the antimicrotubular poison vinblastine. The dynamics of appearance, decrease and loss of cell subpopulations were evaluated by correlating cell DNA content to mono-multinuclearity resulting from cell fusion and division process alteration and to the peculiarities of cell death events.
Results: DNA microfluorimetry and morphological techniques (phase contrast, fluorescence and TEM microscopies) indicated that PEG treatment induced a 4-24c cell increase and the appearance of new giant elements (64-140c DNA content). Ultrastructural analysis and autophagosomal-lysosomal compartment fluorochromization, which allowed us to correlate cytoplasmic changes to death events, indicated that cell depletion occurred through distinct mechanisms: apoptotic death involved 2c, 4c and 8c cells, while autophagic-like death involved intermediate 12-24c cells, showing nuclear (lobulation/micronucleation) and autophagic cytoplasm alterations. Death, spontaneously occurring, especially in intermediate-sized cells, was increased after vinblastine treatment. No evident cell loss by death events was detected in the 64-140c range.
Conclusions: PEG-treated NIHs cultures can represent a model of heterogeneous subpopulations originating from cell fusion and division process anomalies. Altogether, our results suggest that the different cell dynamics of NIHs subpopulations can affect the variability of responses to stimuli able to induce cell degeneration and death. Apoptptic, autophagic or hybrid forms of cell death can also depend on the DNA content and ability to progress through the cell cycle, which may influence the persistence and fate of polyploid cell descendants, also concerning chemotherapeutic agent action
La disclosure nei Piani della Performance delle Università italiane. Intenti simbolici vs approcci integrati alla gestione
No full textThe disclosure in the Plans of the Performance of the Italian University. Sym-bolic intent vs substantial legitimacy approaches
This research focuses on the Performance Plans drawn up by Italian universities, to un-derstand whether the information conveyed in this documents are able to achieve in-creasing transparency and greater accountability - fulfilling the willingness expressed by recent regulatory attempts - or if, conversely, they constitute a mere symbolic com-pliance effort. The study embraces the legitimacy framework and relies upon a mean-ing oriented content analysis of 66 Performance Plans. The main findings show little strategic value underlying Performance Plans, as well as the persistence of certain in-formation gaps on key areas of strategic importance, offering a noteworthy contribution not restricted to the academic debate, but valuable for policy makers and practitioners
Selective expression of the mRNA encoding the short isoform of the gamma 2 GABA-A receptor subunit in rat pituitary cells.
No full text“Il bilancio sociale nelle Università tra compliance formale e disclosure sostanziale. Un’analisi empirica nel contesto italiano”.
No full textQuesto studio si concentra sulla rendicontazione sociale sviluppata dalle Università italiane, per comprendere se quelle virtuose (che hanno preparato continuativamente un bilancio sociale) abbiano fornito solo informazioni di carattere formale, per scopi di legittimità, o abbiano divulgato una comunicazione in grado di soddisfare i bisogni informativi. La ricerca si avvale di una meaning oriented content analysis per un campione di bilanci sociali redatti da 7 Università per il periodo 2010-2012. I risultati evidenziano la persistenza di problemi d’incoerenza nell’informativa, ancora orientata verso la legittimità formale piuttosto che mossa dal genuino desiderio di stabilire un flusso di dialogo con gli stakeholders
Lettera di Alessandra
No full textUn ritratto critico dell'opera di Alessandra Carnaroli, autrice fra le più apprezzate delle ultime generazioni della poesia di ricerca. La sezione a lei dedicata, nel numero della rivista, contiene inoltre saggi di Cecilia Bello Minciacchi, Andrea Cortellessa, e Ivan Schiavone; e vari inediti dell'autrice. Il saggio è pubblicato con lo pseudonimo di Tommaso Ottonieri.A critical portrait of the work of Alessandra Carnaroli, author of the most appreciated in the latest generations of italian research poetry. Published under the pseudonym Tommaso Ottonieri
Selected letters of Alessandra Strozzi
No full textThe letters of Alessandra Strozzi provide a vivid and spirited portrayal of life in fifteenth-century Florence. Among the richest autobiographical materials to survive from the Italian Renaissance, the letters reveal a woman who fought stubbornly to preserve her family's property and position in adverse circumstances, and who was an acute observer of Medicean society. Her letters speak of political and social status, of the concept of honor, and of the harshness of life, including the plague and the loss of children. They are also a guide to Alessandra's inner life over a period of twenty-three years, revealing the pain and sorrow, and, more rarely, the joy and triumph, with which she responded to the events unfolding around her.This edition includes translations, in full or in part, of 35 of the 73 extant letters. The selections carry forward the story of Alessandra's life and illustrate the range of attitudes, concerns, and activities which were characteristic of their author
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