4,081 research outputs found

    A Discussion About Writing Fiction and Creative Prose with Isabel Huggan

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    Award-winning Canadian author Isabel Huggan talks to students about writing, with a focus on fiction and creative non-fiction.Presentation for English 2905 (Introduction to Creative Writing), taught by Dr. Stepanie McKenzie

    Humanismo y Reforma en la corte renacentista de Isabel de Vilamarí : Escipión Capece y sus lectoras

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    Durante la primera mitad del siglo XVI y en la corte salernitana del último príncipe de la casa Sanseverino y de su esposa, Isabel de Vilamarí (noble señora de origen catalán) se desarrolló un intenso clima intelectual. Allí se congregaron artistas y humanistas italianos y españoles. En este ambiente de intercambio cultural, atento en participar en las ideas de la Reforma que se difundió en Nápoles gracias a B. Ochino y a Valdés, nace el poema De principiis rerum del último académico pontaniano: Escipión Capece. En esta obra no sólo se rastrean motivos lucrecianos y virgilianos sino también el influjo de los tratados cosmológicos de Pontano. En este estudio, la autora propone el análisis de la figura y de la obra de Capece a través de sus lectoras: Isabel de Vilamarí y las mujeres cultas de su corte.During the first half of sixteenth century and in the Salernitan court of the last prince Sanseverino and his wife Isabel de Vilamarí (a lady coming from a noble Catalan family) an intense intellectual climate developed. Italian and Spanish artists and humanists met there. In this environment of cultural exchange, that shared in the Reform ideas divulged in Naples by B. Ochino and Valdés, Scipione Capece (the last member of the Pontanian Academy) writes his poem De principiis rerum. In his book Capece uses Latin literature (Vergil and Lucretius mainly) and Pontano's treatises on cosmology. The author of this paper studies Scipione Capece through his female readership: Isabel de Vilamarí and the learned women from her court

    Humanismo y Reforma en la corte renacentista de Isabel de Vilamarí : Escipión Capece y sus lectoras

    No full text
    Durante la primera mitad del siglo XVI y en la corte salernitana del último príncipe de la casa Sanseverino y de su esposa, Isabel de Vilamarí (noble señora de origen catalán) se desarrolló un intenso clima intelectual. Allí se congregaron artistas y humanistas italianos y españoles. En este ambiente de intercambio cultural, atento en participar en las ideas de la Reforma que se difundió en Nápoles gracias a B. Ochino y a Valdés, nace el poema De principiis rerum del último académico pontaniano: Escipión Capece. En esta obra no sólo se rastrean motivos lucrecianos y virgilianos sino también el influjo de los tratados cosmológicos de Pontano. En este estudio, la autora propone el análisis de la figura y de la obra de Capece a través de sus lectoras: Isabel de Vilamarí y las mujeres cultas de su corte.During the first half of sixteenth century and in the Salernitan court of the last prince Sanseverino and his wife Isabel de Vilamarí (a lady coming from a noble Catalan family) an intense intellectual climate developed. Italian and Spanish artists and humanists met there. In this environment of cultural exchange, that shared in the Reform ideas divulged in Naples by B. Ochino and Valdés, Scipione Capece (the last member of the Pontanian Academy) writes his poem De principiis rerum. In his book Capece uses Latin literature (Vergil and Lucretius mainly) and Pontano's treatises on cosmology. The author of this paper studies Scipione Capece through his female readership: Isabel de Vilamarí and the learned women from her court

    The discovery of SycO reveals a new function for type three secretion effector chaperones

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    The Type Three Secretion (T3S) system is a device used by many Gram-negative pathogens that allows bacteria to deliver effector proteins straight into the eukaryotic cell cytosol. These effectors interfere with various signaling pathways to subvert the host cell functions. The secretion machinery of the T3S system consist of a basal body spanning the bacterial inner and outer membrane followed by a stiff hollow needle outside the bacterium. The fully assembled secretion apparatus constitute a continuous hollow conduit that connects the bacteria to the eukaryotic target cell. After cell contact, virulence proteins -called effectors- are injected directly into the cytosol of the host cell via the T3S apparatus. Several effectors of the T3S system require the assistance of specific cytosolic chaperones to be efficiently exported. There are three classes of T3S chaperones. Effector proteins are assisted by Class I chaperones. Although Class I chaperones are well characterized, their main function is still a matter of controversy. In this thesis, we demonstrate that orf155 encodes a specific chaperone for the effector YopO that we called SycO. We showed that SycO enhances YopO secretion in vitro and is required for translocation of YopO into infected cells. By pulldown assay we demonstrated that residues 20 to 77 of YopO are required and sufficient for SycO binding. Using crosslinking experiments and size exclusion chromatography analysis, we determined the stoichiometry of purified SycO and YopO-SycO complexes. SycO alone forms dimers in solution and the YopO-SycO complex has a 1:2 stoichiometry. These results suggested that SycO is a typical chaperone of the Class I. YopO is a serine/theronine kinase that interacts with Rho and Rac and disrupts the cytoskeleton of the target cells. YopO has been shown to localize at the cell plasma-membrane. By transfection of YopO-EGFP hybrid proteins into HEK293T cells, we demonstrated that the chaperone-binding domain (CBD) coincides with the membrane localization domain of YopO. Nevertheless, the CBD was not needed for the kinase activity of YopO. By ultracentrifugation, we also showed that the CBD causes YopO aggregation in the bacteria, when SycO does not cover it. Further, we show that the CBD of YopE and YopT also caused aggregation in the bacteria in the absence of SycE and SycT respectively. YopE, YopT and T3S effectors in other systems also act at the membrane of the eukaryotic host cell. We propose a new hypothesis concerning the role of T3S chaperones. The sub-cellular localization domain of effectors is aggregation-prone and creates the need for a chaperone inside bacteria. We propose that masking such aggregation-prone localization domains may be a general function for type III effector chaperones

    El Tlacuache Núm. 144 (2004). 144 Año 4 (2004) octubre. El Tlacuache

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    -De cantares, refranes, cementerios y epitafios por Isabel Garza Gómez. - El Yauhtli por Margarita Avilés y Macrina Fuentes. - De ofrendas y cosechas por Isabel Garza Gómez

    Biochemical investigation of Orai1 mutants affecting Ca2+ selectivity of Orai1

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    Author: Isabel Simmer, BScMasterarbeit Johannes Kepler Universität Linz 2025Arbeit gesperr
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